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The map label for this gene is IS1086 [H]

Identifier: 158316243

GI number: 158316243

Start: 5332965

End: 5334365

Strand: Reverse

Name: IS1086 [H]

Synonym: Franean1_4467

Alternate gene names: 158316243

Gene position: 5334365-5332965 (Counterclockwise)

Preceding gene: 158316244

Following gene: 158316242

Centisome position: 59.39

GC content: 72.66

Gene sequence:

>1401_bases
ATGGGACGGCCGGCGGATTGGACGCAGACGGTCACGGGGCGGGCGGGGATGTGCTCGCCGGGGCGGCCGCCGGTGGCGCG
GTGGGAGCATCAGCAGCGGTTCTGGGCCGCGGTCGCGCGTGGGCTGAGCAGCGAGGATGCCGGTGTCGCGGCCGGCGTGT
CGCCGGCGGTCGGGACCCGGTGGTTCCGCAACTGTGGCGGGATGCCCCCTTCGGACTTCCCCGCCCCGTCGGGCCGATAC
CTGTCGTTCGCGGAGCGGGAGGAGATCGCCCTGGGTCGTGCCCGCGGGGACAGCATCCGCCGGATCGCGCGGGGTTTGGG
CCGGCCTGCGTCGACGGTGTCACGGGAGCTGCGGCGGAACGCCGGTACCCGCGGCGGGACCCTGGTCTACCGGGCCACGC
TCGCGCAGTGGCATCGAGACCGTCGGGCTGCCCGGCCCAAGACTGCCAAGCTCGCAGGCAACGAGCGGCTGCGTGGCTAT
GTGCAGGACCGGCTCGCCGGGCCGGTCCTGCGCGGCGACGGCACGGTGGTGCCCGGCCCGTGGACGGCGCCGTTCACCGG
CAGGAACAAGCCGCACCGCCAGGACCGCCGTTGGGCCAGCGCGTGGAGCCCGGAGCAGATCTCGCGGCGGCTGCGGGTCG
ATTTTCCCGACGACCCGGCGATGCGGATCTCGCATGAGGCGATCTACCAGGCCCTGTACATCGAGAGGCGGGGGGCGTTG
CGCCGTGAGCTGGTCGCGTGCCTGCGTACGGGCCGCGCCCTGCGGGTGCCACGCGCGCGGGCCGGACGCCGCCCCGACGG
CATGGTCACCCCCGAGGTGCGGATCGGAGCCCGGCCTGTTGAGGCCACAGACCGGGCGGTCGCGGGGCACTGGGAAGGCG
ACCTGATCATCGGGTTGAACCGGTCCGCGATCGGCACGCTGGTCGAGCGCACCACCCGGCTCACGGTCCTGCTGCACCTG
CCCCGCATGGACGGCTACGGCCATGAACCACGGGTGAAGAACGGTCCGGCGTTGGCAGGCCGCGGCGCCGACGCTGTCCG
GGACGCGATCACACGAGCGTTCGCGGAGCTGCCCGAGCAGCTACGGCGGACCCTGACCTGGGACCGCGGCAAGGAGATGG
CCGGACACGCCGCGCTGACCGCCGACACGGGCCTGGGGGTCTACTTCGCCGACCCGCACAGCCCCTGGCAGCGCGGCACG
AACGAGAACACCAACGGGCTGCTACGCCAGTACTTCCCCAAGGGCACCGACCTGTCCCGCTGGACCCGCCACGAACTCGC
CACCATCGCCGCGACCCTCAACGACCGGCCCCGCAAGACCCTCGACTGGAAGACCCCCACCGAAGCGATGAACAACCAGC
TACTCTCACTTCAACAACCCGGTGTTGCGAGGACCGGTTGA

Upstream 100 bases:

>100_bases
GTAACCACCAGCTCACCGACGGGGCGATTTTTCATTCGGACCGCGGAAGCAACGGCGGATTCAATTGGTCGTCGCAACAC
CTCGATCCGGAGGTGTGGGA

Downstream 100 bases:

>100_bases
GTTCGGTAACTATACGTCTACCCAGTTCGCTGCTACGTTGAAGAACCTCGGGATCCGCCAGTCCGTCGGCCGGACCGGAA
TTTGCTATGACAATGCGATG

Product: integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGRPADWTQTVTGRAGMCSPGRPPVARWEHQQRFWAAVARGLSSEDAGVAAGVSPAVGTRWFRNCGGMPPSDFPAPSGRY
LSFAEREEIALGRARGDSIRRIARGLGRPASTVSRELRRNAGTRGGTLVYRATLAQWHRDRRAARPKTAKLAGNERLRGY
VQDRLAGPVLRGDGTVVPGPWTAPFTGRNKPHRQDRRWASAWSPEQISRRLRVDFPDDPAMRISHEAIYQALYIERRGAL
RRELVACLRTGRALRVPRARAGRRPDGMVTPEVRIGARPVEATDRAVAGHWEGDLIIGLNRSAIGTLVERTTRLTVLLHL
PRMDGYGHEPRVKNGPALAGRGADAVRDAITRAFAELPEQLRRTLTWDRGKEMAGHAALTADTGLGVYFADPHSPWQRGT
NENTNGLLRQYFPKGTDLSRWTRHELATIAATLNDRPRKTLDWKTPTEAMNNQLLSLQQPGVARTG

Sequences:

>Translated_466_residues
MGRPADWTQTVTGRAGMCSPGRPPVARWEHQQRFWAAVARGLSSEDAGVAAGVSPAVGTRWFRNCGGMPPSDFPAPSGRY
LSFAEREEIALGRARGDSIRRIARGLGRPASTVSRELRRNAGTRGGTLVYRATLAQWHRDRRAARPKTAKLAGNERLRGY
VQDRLAGPVLRGDGTVVPGPWTAPFTGRNKPHRQDRRWASAWSPEQISRRLRVDFPDDPAMRISHEAIYQALYIERRGAL
RRELVACLRTGRALRVPRARAGRRPDGMVTPEVRIGARPVEATDRAVAGHWEGDLIIGLNRSAIGTLVERTTRLTVLLHL
PRMDGYGHEPRVKNGPALAGRGADAVRDAITRAFAELPEQLRRTLTWDRGKEMAGHAALTADTGLGVYFADPHSPWQRGT
NENTNGLLRQYFPKGTDLSRWTRHELATIAATLNDRPRKTLDWKTPTEAMNNQLLSLQQPGVARTG
>Mature_465_residues
GRPADWTQTVTGRAGMCSPGRPPVARWEHQQRFWAAVARGLSSEDAGVAAGVSPAVGTRWFRNCGGMPPSDFPAPSGRYL
SFAEREEIALGRARGDSIRRIARGLGRPASTVSRELRRNAGTRGGTLVYRATLAQWHRDRRAARPKTAKLAGNERLRGYV
QDRLAGPVLRGDGTVVPGPWTAPFTGRNKPHRQDRRWASAWSPEQISRRLRVDFPDDPAMRISHEAIYQALYIERRGALR
RELVACLRTGRALRVPRARAGRRPDGMVTPEVRIGARPVEATDRAVAGHWEGDLIIGLNRSAIGTLVERTTRLTVLLHLP
RMDGYGHEPRVKNGPALAGRGADAVRDAITRAFAELPEQLRRTLTWDRGKEMAGHAALTADTGLGVYFADPHSPWQRGTN
ENTNGLLRQYFPKGTDLSRWTRHELATIAATLNDRPRKTLDWKTPTEAMNNQLLSLQQPGVARTG

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=409, Percent_Identity=39.6088019559902, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1787671, Length=409, Percent_Identity=39.6088019559902, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1786450, Length=409, Percent_Identity=39.3643031784841, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 51615; Mature: 51484

Theoretical pI: Translated: 11.86; Mature: 11.86

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRPADWTQTVTGRAGMCSPGRPPVARWEHQQRFWAAVARGLSSEDAGVAAGVSPAVGTR
CCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
WFRNCGGMPPSDFPAPSGRYLSFAEREEIALGRARGDSIRRIARGLGRPASTVSRELRRN
HHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHH
AGTRGGTLVYRATLAQWHRDRRAARPKTAKLAGNERLRGYVQDRLAGPVLRGDGTVVPGP
CCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCEECCCCEECCCC
WTAPFTGRNKPHRQDRRWASAWSPEQISRRLRVDFPDDPAMRISHEAIYQALYIERRGAL
CCCCCCCCCCCCHHHHHHHCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
RRELVACLRTGRALRVPRARAGRRPDGMVTPEVRIGARPVEATDRAVAGHWEGDLIIGLN
HHHHHHHHHCCCEEECCHHHCCCCCCCCCCCCCEECCCCCCCCCCCEECCCCCCEEEECC
RSAIGTLVERTTRLTVLLHLPRMDGYGHEPRVKNGPALAGRGADAVRDAITRAFAELPEQ
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LRRTLTWDRGKEMAGHAALTADTGLGVYFADPHSPWQRGTNENTNGLLRQYFPKGTDLSR
HHHHHCHHCCHHHCCCEEEEECCCCEEEECCCCCHHHCCCCCCCCHHHHHHCCCCCCHHH
WTRHELATIAATLNDRPRKTLDWKTPTEAMNNQLLSLQQPGVARTG
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHEECCCCCCCCCC
>Mature Secondary Structure 
GRPADWTQTVTGRAGMCSPGRPPVARWEHQQRFWAAVARGLSSEDAGVAAGVSPAVGTR
CCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
WFRNCGGMPPSDFPAPSGRYLSFAEREEIALGRARGDSIRRIARGLGRPASTVSRELRRN
HHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHH
AGTRGGTLVYRATLAQWHRDRRAARPKTAKLAGNERLRGYVQDRLAGPVLRGDGTVVPGP
CCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHCCCEECCCCEECCCC
WTAPFTGRNKPHRQDRRWASAWSPEQISRRLRVDFPDDPAMRISHEAIYQALYIERRGAL
CCCCCCCCCCCCHHHHHHHCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
RRELVACLRTGRALRVPRARAGRRPDGMVTPEVRIGARPVEATDRAVAGHWEGDLIIGLN
HHHHHHHHHCCCEEECCHHHCCCCCCCCCCCCCEECCCCCCCCCCCEECCCCCCEEEECC
RSAIGTLVERTTRLTVLLHLPRMDGYGHEPRVKNGPALAGRGADAVRDAITRAFAELPEQ
HHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LRRTLTWDRGKEMAGHAALTADTGLGVYFADPHSPWQRGTNENTNGLLRQYFPKGTDLSR
HHHHHCHHCCHHHCCCEEEEECCCCEEEECCCCCHHHCCCCCCCCHHHHHHCCCCCCHHH
WTRHELATIAATLNDRPRKTLDWKTPTEAMNNQLLSLQQPGVARTG
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1334071 [H]