Click here to switch to the map view.

The map label for this gene is bhbA [H]

Identifier: 158316092

GI number: 158316092

Start: 5148085

End: 5149662

Strand: Reverse

Name: bhbA [H]

Synonym: Franean1_4310

Alternate gene names: 158316092

Gene position: 5149662-5148085 (Counterclockwise)

Preceding gene: 158316093

Following gene: 158316091

Centisome position: 57.33

GC content: 70.22

Gene sequence:

>1578_bases
ATGAGCGATTCTGTCCAGACCAACTCCGGCCTGCCGGTGGAGCCGGTGTACGGGCCGGCGGACCGCGGCACCGAACCGCC
CGCGCCGGGCAAGTTTCCGTTCACCCGCGGCAACTACGCCTCCGGGTACCGCGGGCGCACGTGGACGTTCCGCCAGTACT
CCGGCTTCGGCACCGCCGAGGAGTCCAACAACCGCTACCGCTACCTGCTCGAGCAGGGCGGCACGGGCCTGTCGGTGGCC
CTCGACCTGCCCACCCAGTGCGGGTACGACTCCGACGACCCGGAGGTGACCGAGGAGGTCGGCCGGGTCGGCGTCGCCGT
CGACACGCTCGCCGACGCCGAGGTGCTCTTCGAGGGCATCCCGCTGGACAAGATCAGCACCAGCTTCACGATCAACGGCA
CCGCCGCGATCCTGCTGGCCTTCTACGTGGCGGCCGCGGAGCGCTCCGGGGTGCCCCGGGCCAAGCTGACCGGGACGATC
CAGAACGACATCCTCAAGGAGTACGCCTCCCGCGGGACGTGGATCTGGCCGCCGGAGCCGTCGCTGCGCCTGATCGCCGA
CACCATCGAGTTCTGCGCCGAGGAGGTGCCGCGCTTCAACGCGATCTCCGTGGCCGGCGCGCACTTCCGGGACGCCGGCG
CGAACGCGGTACAGGAGATGGCGTTCACCCTCGCCGACGGCGTCACCTACTGCGACACGGTGATCGAGCGCGGTCGGCTG
TCGATCGAGAAGTTCGCGCCGCAGGTGTCGTTCTTCTTCTACACCCACGGCGACTTCTTCGAGGAGATCGCCAAGTACCG
GGCGGGCCGGCGGCGGTGGGCGACCATCGTCCGGGAGCGCTACGGCGCCACCGCCGACAAGGCGTCGATGTTCCGCTTCG
GCTGCGTGGCCGGCGGGGCGTCGCTGTACGCGCCGCAGGCGCAGAACAACATCGTCCGGGTCGCCTACGAGGCGATGGCC
TCGGTGCTCGGCGGCGTGCAGTCGATGTTCACCGCGGCCTGGGACGAGCCGTTCGCGCTGCCCAGCGAGGAGTCGGCGAC
GCTCGCGCTGCGCACCCAGCAGATCCTCGCCTACGAGACGGGGGTGACCCGCACCGCCGACCCGCTGGGCGGCTCGTACT
TCGTCGAGGCGCTCACCGACGCCACCGAGGCCCGCATCATCGAGATCATGGACGACCTCGAGCAGCACGGCGGCATGGTC
CGCGCGATCGAGGACGGCTACCTCCAGGGCATGATCGCCGACGAGGCGTACCAGCTGCACCAGGACATCGAGAGCGGAAA
GGTGCCGATCGTCGGCGTGAACCGGTTCGTCTCCGACGAGCCGGCGCCCGACCTCGCCACCTACGAACTGGACGCCGAAG
GCCGCGAGCGGCAGCTGAAGCGGCTGGCGAAGGTCAAGGGCGAGCGCGACGCCGCGGCCGTCCGGGCCAGCCTCGACGCG
CTGGCCCGCGCCGCGGAGGGGAACGGGAACCTGATGCACAACCTGATCGACTGCGCCAACGCCTACTGCACGGTGGGCGA
GATGGTCGCCACCCTCAAGAACGTCTGGGGCGAGTTCCAGCAGCCGGTGGTGTTCTGA

Upstream 100 bases:

>100_bases
GCCGCCGGCAGCCGGCGCGCGGCCGGCACCCGCTGAGCGCCGCGGTCCCGGGCCCGGCCCCGGTGTCGACGTTCCGTCTC
CAGAAAGTGTCGAAGCGACG

Downstream 100 bases:

>100_bases
TGCCGGCGCGGGTACTGGTCGCCAAGCCCGGCCTGGACGGGCACGACCGCGGGGCCAAGATCGTCGCGCGGGCGTTGCGC
GACGCCGGCTTCGAGGTCGT

Product: methylmalonyl-CoA mutase, large subunit

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSDSVQTNSGLPVEPVYGPADRGTEPPAPGKFPFTRGNYASGYRGRTWTFRQYSGFGTAEESNNRYRYLLEQGGTGLSVA
LDLPTQCGYDSDDPEVTEEVGRVGVAVDTLADAEVLFEGIPLDKISTSFTINGTAAILLAFYVAAAERSGVPRAKLTGTI
QNDILKEYASRGTWIWPPEPSLRLIADTIEFCAEEVPRFNAISVAGAHFRDAGANAVQEMAFTLADGVTYCDTVIERGRL
SIEKFAPQVSFFFYTHGDFFEEIAKYRAGRRRWATIVRERYGATADKASMFRFGCVAGGASLYAPQAQNNIVRVAYEAMA
SVLGGVQSMFTAAWDEPFALPSEESATLALRTQQILAYETGVTRTADPLGGSYFVEALTDATEARIIEIMDDLEQHGGMV
RAIEDGYLQGMIADEAYQLHQDIESGKVPIVGVNRFVSDEPAPDLATYELDAEGRERQLKRLAKVKGERDAAAVRASLDA
LARAAEGNGNLMHNLIDCANAYCTVGEMVATLKNVWGEFQQPVVF

Sequences:

>Translated_525_residues
MSDSVQTNSGLPVEPVYGPADRGTEPPAPGKFPFTRGNYASGYRGRTWTFRQYSGFGTAEESNNRYRYLLEQGGTGLSVA
LDLPTQCGYDSDDPEVTEEVGRVGVAVDTLADAEVLFEGIPLDKISTSFTINGTAAILLAFYVAAAERSGVPRAKLTGTI
QNDILKEYASRGTWIWPPEPSLRLIADTIEFCAEEVPRFNAISVAGAHFRDAGANAVQEMAFTLADGVTYCDTVIERGRL
SIEKFAPQVSFFFYTHGDFFEEIAKYRAGRRRWATIVRERYGATADKASMFRFGCVAGGASLYAPQAQNNIVRVAYEAMA
SVLGGVQSMFTAAWDEPFALPSEESATLALRTQQILAYETGVTRTADPLGGSYFVEALTDATEARIIEIMDDLEQHGGMV
RAIEDGYLQGMIADEAYQLHQDIESGKVPIVGVNRFVSDEPAPDLATYELDAEGRERQLKRLAKVKGERDAAAVRASLDA
LARAAEGNGNLMHNLIDCANAYCTVGEMVATLKNVWGEFQQPVVF
>Mature_524_residues
SDSVQTNSGLPVEPVYGPADRGTEPPAPGKFPFTRGNYASGYRGRTWTFRQYSGFGTAEESNNRYRYLLEQGGTGLSVAL
DLPTQCGYDSDDPEVTEEVGRVGVAVDTLADAEVLFEGIPLDKISTSFTINGTAAILLAFYVAAAERSGVPRAKLTGTIQ
NDILKEYASRGTWIWPPEPSLRLIADTIEFCAEEVPRFNAISVAGAHFRDAGANAVQEMAFTLADGVTYCDTVIERGRLS
IEKFAPQVSFFFYTHGDFFEEIAKYRAGRRRWATIVRERYGATADKASMFRFGCVAGGASLYAPQAQNNIVRVAYEAMAS
VLGGVQSMFTAAWDEPFALPSEESATLALRTQQILAYETGVTRTADPLGGSYFVEALTDATEARIIEIMDDLEQHGGMVR
AIEDGYLQGMIADEAYQLHQDIESGKVPIVGVNRFVSDEPAPDLATYELDAEGRERQLKRLAKVKGERDAAAVRASLDAL
ARAAEGNGNLMHNLIDCANAYCTVGEMVATLKNVWGEFQQPVVF

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=518, Percent_Identity=44.2084942084942, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1789284, Length=524, Percent_Identity=44.4656488549618, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25144500, Length=516, Percent_Identity=45.1550387596899, Blast_Score=457, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 57216; Mature: 57084

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSVQTNSGLPVEPVYGPADRGTEPPAPGKFPFTRGNYASGYRGRTWTFRQYSGFGTAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
ESNNRYRYLLEQGGTGLSVALDLPTQCGYDSDDPEVTEEVGRVGVAVDTLADAEVLFEGI
CCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCC
PLDKISTSFTINGTAAILLAFYVAAAERSGVPRAKLTGTIQNDILKEYASRGTWIWPPEP
CHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHCCCEECCCCC
SLRLIADTIEFCAEEVPRFNAISVAGAHFRDAGANAVQEMAFTLADGVTYCDTVIERGRL
CHHHHHHHHHHHHHHCCCCCEEEECCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCC
SIEKFAPQVSFFFYTHGDFFEEIAKYRAGRRRWATIVRERYGATADKASMFRFGCVAGGA
CHHHHCCCEEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
SLYAPQAQNNIVRVAYEAMASVLGGVQSMFTAAWDEPFALPSEESATLALRTQQILAYET
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHC
GVTRTADPLGGSYFVEALTDATEARIIEIMDDLEQHGGMVRAIEDGYLQGMIADEAYQLH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
QDIESGKVPIVGVNRFVSDEPAPDLATYELDAEGRERQLKRLAKVKGERDAAAVRASLDA
HHHHCCCCEEEECHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LARAAEGNGNLMHNLIDCANAYCTVGEMVATLKNVWGEFQQPVVF
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDSVQTNSGLPVEPVYGPADRGTEPPAPGKFPFTRGNYASGYRGRTWTFRQYSGFGTAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
ESNNRYRYLLEQGGTGLSVALDLPTQCGYDSDDPEVTEEVGRVGVAVDTLADAEVLFEGI
CCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCC
PLDKISTSFTINGTAAILLAFYVAAAERSGVPRAKLTGTIQNDILKEYASRGTWIWPPEP
CHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHCCCEECCCCC
SLRLIADTIEFCAEEVPRFNAISVAGAHFRDAGANAVQEMAFTLADGVTYCDTVIERGRL
CHHHHHHHHHHHHHHCCCCCEEEECCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCC
SIEKFAPQVSFFFYTHGDFFEEIAKYRAGRRRWATIVRERYGATADKASMFRFGCVAGGA
CHHHHCCCEEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
SLYAPQAQNNIVRVAYEAMASVLGGVQSMFTAAWDEPFALPSEESATLALRTQQILAYET
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHC
GVTRTADPLGGSYFVEALTDATEARIIEIMDDLEQHGGMVRAIEDGYLQGMIADEAYQLH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
QDIESGKVPIVGVNRFVSDEPAPDLATYELDAEGRERQLKRLAKVKGERDAAAVRASLDA
HHHHCCCCEEEECHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LARAAEGNGNLMHNLIDCANAYCTVGEMVATLKNVWGEFQQPVVF
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]