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The map label for this gene is liaS [H]

Identifier: 158316027

GI number: 158316027

Start: 5053929

End: 5055716

Strand: Reverse

Name: liaS [H]

Synonym: Franean1_4245

Alternate gene names: 158316027

Gene position: 5055716-5053929 (Counterclockwise)

Preceding gene: 158316029

Following gene: 158316026

Centisome position: 56.29

GC content: 76.17

Gene sequence:

>1788_bases
GTGTCGACGATCGTGATCGGCCCGGCAACGGACCGCTGGGGCCGGTACCGGTTACCGTCGGCGGCCCTCGCGCTCGGCGC
GGTGGCGGGCGGTCTCGCCCTCGCCGCCGGATGGCCGTACTGGCGTGCCGAGCCCGCGGCGGCCGCGGTCAACGCCTTCG
TGACCGGCGGGTTCGTCACCTCCGGGATCCTGCTCGCCAGCCTCGCCGTGCTCCGCCGCACCGCTCTGCTCGCCGTCGCG
GCCGGGCTGTGCTGGGCCGCCACCTGGCTGCTGTGTCGGGAGGTCGGACCGCTGCCGGCGATCGGCCTGTTCGCGAACGC
GCTGTTCTTCGTCGTGCTCGGCTGGGGGGTGCTGCTCTACCCGGACGGGCGGCTCACCGGACGGGCGGACTGGTGGGCGG
CCATGGCCACCACCGTGGTGTTCGGCCCGCTGGCCGTGGTGGCGATCATCGTGGCCGAACCGGAGTGGAACGGGTTCGCG
CCGGACGTCCGCTGGCCGTCCCTGCTGCCCGACGAAGCCGTGTTCGGGTTCGTCAGAGACCTCTACACGGTCATCAACCC
GGTGCTCGCGGTGGTGTTCGTCGGCCTGCTCGTGCGGCGCTGCGCCCGGCTCTACGGGCTGGAGCGCTCGCTGATCGCGC
CCGCGCTCGCGGTGCTGGGCGTCGTCGTGCTCTGCCTGGCGGCGCTGTCACCCACGCTGGAGAACCACCGGCCTGGGCAC
GTCGCCGACGGTATCGCCGCGCAGGGCGCCGCGCTGGCGGTGTTCCCGCTCGCGCTGCTCGTCGCGATGGTGCGGCGCGC
CGTCGCCGCGGCGACGGCGGCCGATCGCATGCTGCGTCTCGGCGGCGGCCCGGCGACGGTCGGCGCCGTCCGGGACGCGC
TGCGCAGCGTGCTGCGCGACGGCACGGTGGACGTCTTCTTCTGGCTGCCCGAGGAGCATCGTTTCGTCGACGTCGACGGG
GAGGCGGCCGACCCGGACGGGCGGCCGCAGGCCTGGCGCCAGGAGGTGACGGCGACCTCGGGGGAGCGGCTCGCGGTGGT
GACCGGCGGGCCGCTGCTGCTTCGGCACGTGCCGTTCACCGAGGCCGCGCTGCGGGCCGCGCGGCTGGCGCTGCAGAACG
CGCAGCTCCAGGTCGCCATCCAGGCGGAGCTGGTGGAGACCCGGGCTGCCCAGCGGCGCGTCGAGGAGGCCGAGGCCGCC
GAGCGCAACCGCATCGCGCGTGACCTGCACGACGGTGTGCAGCAGCGCCTGCTCGCGCTGGCGATGTCGGCGAGCGCGGC
GGAGTGGTCCACCGACGACGACCCGGACAGCACGGCATCGGTGTTCCGGGACATCCGGGCGGGGCTGCTGGCGGCGAACG
CGGAGGTCCGCAACGTCGCCCGCGGCCTGGACCCGGAGATCCTGCGTACCGCCGGCCTGGCCGGGGCGGTCCGGGACGCG
GCCGGCCGCCTGGGGCTGCCCGTGGACCTGCGCGTCGAGCTCACCGGCACCGCCCCCTACGGCGACCTCGCCGAGCGCAC
GGTGTACTTCGCGGTCTGCGAGGCGCTGGCGAACGTGGCGAAGCACTCCGGGGCCGGCGCTGTGTGGGTGCGGCTGACCG
GCGGTGAGGCCCGGCTCGAGGCCGAGGTCCGCGACGACGGCCGCGGCGGCGCCGCGGCCGGCACCGCCGGGGGCCTGGCC
GGCCTGCGGGACCGGGCCGACGCCGCGGGTGGGAGCATGCTGCTGACCAGCCCGCCCGGTGCCGGGACGACGGTACTTGT
CAGCGTCCCCGTCGGCGGTGCGCGCTGA

Upstream 100 bases:

>100_bases
GTGCGACGATCGCCACGCAGAGCGGCGGCTTCGGTGACCGCGCTGTGAACGCGTAACTGCACAGCCGCCCACGCCACGTG
GCGGCCGGCGGAGGGGATCC

Downstream 100 bases:

>100_bases
CGCCGCGCCCGGGGTACGTGGTGTACCCGTTGGCACGGTTGGTCCGGCGGGGGTGGGCGGTGGCGCGTCCGCTGCTGCCG
GCGGTGGGCGCCGGTGCCGC

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MSTIVIGPATDRWGRYRLPSAALALGAVAGGLALAAGWPYWRAEPAAAAVNAFVTGGFVTSGILLASLAVLRRTALLAVA
AGLCWAATWLLCREVGPLPAIGLFANALFFVVLGWGVLLYPDGRLTGRADWWAAMATTVVFGPLAVVAIIVAEPEWNGFA
PDVRWPSLLPDEAVFGFVRDLYTVINPVLAVVFVGLLVRRCARLYGLERSLIAPALAVLGVVVLCLAALSPTLENHRPGH
VADGIAAQGAALAVFPLALLVAMVRRAVAAATAADRMLRLGGGPATVGAVRDALRSVLRDGTVDVFFWLPEEHRFVDVDG
EAADPDGRPQAWRQEVTATSGERLAVVTGGPLLLRHVPFTEAALRAARLALQNAQLQVAIQAELVETRAAQRRVEEAEAA
ERNRIARDLHDGVQQRLLALAMSASAAEWSTDDDPDSTASVFRDIRAGLLAANAEVRNVARGLDPEILRTAGLAGAVRDA
AGRLGLPVDLRVELTGTAPYGDLAERTVYFAVCEALANVAKHSGAGAVWVRLTGGEARLEAEVRDDGRGGAAAGTAGGLA
GLRDRADAAGGSMLLTSPPGAGTTVLVSVPVGGAR

Sequences:

>Translated_595_residues
MSTIVIGPATDRWGRYRLPSAALALGAVAGGLALAAGWPYWRAEPAAAAVNAFVTGGFVTSGILLASLAVLRRTALLAVA
AGLCWAATWLLCREVGPLPAIGLFANALFFVVLGWGVLLYPDGRLTGRADWWAAMATTVVFGPLAVVAIIVAEPEWNGFA
PDVRWPSLLPDEAVFGFVRDLYTVINPVLAVVFVGLLVRRCARLYGLERSLIAPALAVLGVVVLCLAALSPTLENHRPGH
VADGIAAQGAALAVFPLALLVAMVRRAVAAATAADRMLRLGGGPATVGAVRDALRSVLRDGTVDVFFWLPEEHRFVDVDG
EAADPDGRPQAWRQEVTATSGERLAVVTGGPLLLRHVPFTEAALRAARLALQNAQLQVAIQAELVETRAAQRRVEEAEAA
ERNRIARDLHDGVQQRLLALAMSASAAEWSTDDDPDSTASVFRDIRAGLLAANAEVRNVARGLDPEILRTAGLAGAVRDA
AGRLGLPVDLRVELTGTAPYGDLAERTVYFAVCEALANVAKHSGAGAVWVRLTGGEARLEAEVRDDGRGGAAAGTAGGLA
GLRDRADAAGGSMLLTSPPGAGTTVLVSVPVGGAR
>Mature_594_residues
STIVIGPATDRWGRYRLPSAALALGAVAGGLALAAGWPYWRAEPAAAAVNAFVTGGFVTSGILLASLAVLRRTALLAVAA
GLCWAATWLLCREVGPLPAIGLFANALFFVVLGWGVLLYPDGRLTGRADWWAAMATTVVFGPLAVVAIIVAEPEWNGFAP
DVRWPSLLPDEAVFGFVRDLYTVINPVLAVVFVGLLVRRCARLYGLERSLIAPALAVLGVVVLCLAALSPTLENHRPGHV
ADGIAAQGAALAVFPLALLVAMVRRAVAAATAADRMLRLGGGPATVGAVRDALRSVLRDGTVDVFFWLPEEHRFVDVDGE
AADPDGRPQAWRQEVTATSGERLAVVTGGPLLLRHVPFTEAALRAARLALQNAQLQVAIQAELVETRAAQRRVEEAEAAE
RNRIARDLHDGVQQRLLALAMSASAAEWSTDDDPDSTASVFRDIRAGLLAANAEVRNVARGLDPEILRTAGLAGAVRDAA
GRLGLPVDLRVELTGTAPYGDLAERTVYFAVCEALANVAKHSGAGAVWVRLTGGEARLEAEVRDDGRGGAAAGTAGGLAG
LRDRADAAGGSMLLTSPPGAGTTVLVSVPVGGAR

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=208, Percent_Identity=28.3653846153846, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62017; Mature: 61886

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIVIGPATDRWGRYRLPSAALALGAVAGGLALAAGWPYWRAEPAAAAVNAFVTGGFVT
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHH
SGILLASLAVLRRTALLAVAAGLCWAATWLLCREVGPLPAIGLFANALFFVVLGWGVLLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEE
PDGRLTGRADWWAAMATTVVFGPLAVVAIIVAEPEWNGFAPDVRWPSLLPDEAVFGFVRD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
LYTVINPVLAVVFVGLLVRRCARLYGLERSLIAPALAVLGVVVLCLAALSPTLENHRPGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
VADGIAAQGAALAVFPLALLVAMVRRAVAAATAADRMLRLGGGPATVGAVRDALRSVLRD
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
GTVDVFFWLPEEHRFVDVDGEAADPDGRPQAWRQEVTATSGERLAVVTGGPLLLRHVPFT
CCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECCHH
EAALRAARLALQNAQLQVAIQAELVETRAAQRRVEEAEAAERNRIARDLHDGVQQRLLAL
HHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMSASAAEWSTDDDPDSTASVFRDIRAGLLAANAEVRNVARGLDPEILRTAGLAGAVRDA
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCHHHHHHHH
AGRLGLPVDLRVELTGTAPYGDLAERTVYFAVCEALANVAKHSGAGAVWVRLTGGEARLE
HCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
AEVRDDGRGGAAAGTAGGLAGLRDRADAAGGSMLLTSPPGAGTTVLVSVPVGGAR
EEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
STIVIGPATDRWGRYRLPSAALALGAVAGGLALAAGWPYWRAEPAAAAVNAFVTGGFVT
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHH
SGILLASLAVLRRTALLAVAAGLCWAATWLLCREVGPLPAIGLFANALFFVVLGWGVLLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEE
PDGRLTGRADWWAAMATTVVFGPLAVVAIIVAEPEWNGFAPDVRWPSLLPDEAVFGFVRD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
LYTVINPVLAVVFVGLLVRRCARLYGLERSLIAPALAVLGVVVLCLAALSPTLENHRPGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
VADGIAAQGAALAVFPLALLVAMVRRAVAAATAADRMLRLGGGPATVGAVRDALRSVLRD
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
GTVDVFFWLPEEHRFVDVDGEAADPDGRPQAWRQEVTATSGERLAVVTGGPLLLRHVPFT
CCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECCHH
EAALRAARLALQNAQLQVAIQAELVETRAAQRRVEEAEAAERNRIARDLHDGVQQRLLAL
HHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMSASAAEWSTDDDPDSTASVFRDIRAGLLAANAEVRNVARGLDPEILRTAGLAGAVRDA
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCHHHHHHHH
AGRLGLPVDLRVELTGTAPYGDLAERTVYFAVCEALANVAKHSGAGAVWVRLTGGEARLE
HCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
AEVRDDGRGGAAAGTAGGLAGLRDRADAAGGSMLLTSPPGAGTTVLVSVPVGGAR
EEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]