Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 158315851

GI number: 158315851

Start: 4846549

End: 4847187

Strand: Reverse

Name: tilS [H]

Synonym: Franean1_4066

Alternate gene names: 158315851

Gene position: 4847187-4846549 (Counterclockwise)

Preceding gene: 158315853

Following gene: 158315850

Centisome position: 53.97

GC content: 65.41

Gene sequence:

>639_bases
ATGACAGTGGAACCACGCCGGTACAAAGATCCACACGAAGCCTGGGCGGCCCTCGATACACCCTGGCACAAAGCACTGGA
GATGGCATGGGAGTCGTATCTCGCCGGTGGGGTCGGAGTCGGCGCGATACTGACCGACAGCGGGGGGCGCATCATCGGCT
ACGGCCGAAACCAACGCTTCGCCTCCGCATCTCCTCGCCTGCTTGCCCACGCAGAGATGGAGGCCCTTGCCGCGCTCCCC
CCGGGTAAGGATCGTGCACACGACGCTGTTCTCTACACGACGTTGCACCCATGCCCGATGTGTCTCGGCGCTGTGGTGGT
GGCGCGCATAGGCCAGGTCCGCTTCGGAGCCTTTGATCCGACCTGGCTCGGCATTGAGCACCTGCCGGATCTCAACGAGG
AGGTCCGCCGTCGCTGGCCGGCGGTCATCGGCCCTCTTGCCGGCCCGCTGGGCGAGTGGGCCGCGGTCCTCCCCGCCCTG
AACACGAACGGGTCTCTCCTCCGGGCGATGGAAACGGTCGCGCCACAACGCGCACACCGTGCTCGGGTCATCGCAGATCG
CCTCGAAGCCGACCGACCTGATACGACCAGCCAGGCGCTTGAGCAGGTCTGGGATCTTCTGATCAACTACAACCAGTAG

Upstream 100 bases:

>100_bases
CGACGCCGGTAGGGTCGTGCGTCGGGCCGGCCGCCTGACCGAGTACCCCGCGAGCGTCGAGCATCTGGCATCACCATCAA
CGATTCTCCCCGAGGAGGCC

Downstream 100 bases:

>100_bases
ATAGATCATCGGTCGTAGGCGGGGCTTCCGCTGTACTCGAGTACAGTCGGCCGACTTTACTCTCGGAACCCCGAGCTCAG
GGGTGGCCCGGAACAGGACC

Product: zinc-binding CMP/dCMP deaminase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTVEPRRYKDPHEAWAALDTPWHKALEMAWESYLAGGVGVGAILTDSGGRIIGYGRNQRFASASPRLLAHAEMEALAALP
PGKDRAHDAVLYTTLHPCPMCLGAVVVARIGQVRFGAFDPTWLGIEHLPDLNEEVRRRWPAVIGPLAGPLGEWAAVLPAL
NTNGSLLRAMETVAPQRAHRARVIADRLEADRPDTTSQALEQVWDLLINYNQ

Sequences:

>Translated_212_residues
MTVEPRRYKDPHEAWAALDTPWHKALEMAWESYLAGGVGVGAILTDSGGRIIGYGRNQRFASASPRLLAHAEMEALAALP
PGKDRAHDAVLYTTLHPCPMCLGAVVVARIGQVRFGAFDPTWLGIEHLPDLNEEVRRRWPAVIGPLAGPLGEWAAVLPAL
NTNGSLLRAMETVAPQRAHRARVIADRLEADRPDTTSQALEQVWDLLINYNQ
>Mature_211_residues
TVEPRRYKDPHEAWAALDTPWHKALEMAWESYLAGGVGVGAILTDSGGRIIGYGRNQRFASASPRLLAHAEMEALAALPP
GKDRAHDAVLYTTLHPCPMCLGAVVVARIGQVRFGAFDPTWLGIEHLPDLNEEVRRRWPAVIGPLAGPLGEWAAVLPALN
TNGSLLRAMETVAPQRAHRARVIADRLEADRPDTTSQALEQVWDLLINYNQ

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0590

COG function: function code FJ; Cytosine/adenosine deaminases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI145693172, Length=133, Percent_Identity=36.8421052631579, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002125
- InterPro:   IPR016193
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00383 dCMP_cyt_deam_1; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 23265; Mature: 23134

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEPRRYKDPHEAWAALDTPWHKALEMAWESYLAGGVGVGAILTDSGGRIIGYGRNQRF
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCC
ASASPRLLAHAEMEALAALPPGKDRAHDAVLYTTLHPCPMCLGAVVVARIGQVRFGAFDP
CCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHCCCCCC
TWLGIEHLPDLNEEVRRRWPAVIGPLAGPLGEWAAVLPALNTNGSLLRAMETVAPQRAHR
CCCCHHHCCCCCHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ARVIADRLEADRPDTTSQALEQVWDLLINYNQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVEPRRYKDPHEAWAALDTPWHKALEMAWESYLAGGVGVGAILTDSGGRIIGYGRNQRF
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCC
ASASPRLLAHAEMEALAALPPGKDRAHDAVLYTTLHPCPMCLGAVVVARIGQVRFGAFDP
CCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHCCCCCC
TWLGIEHLPDLNEEVRRRWPAVIGPLAGPLGEWAAVLPALNTNGSLLRAMETVAPQRAHR
CCCCHHHCCCCCHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ARVIADRLEADRPDTTSQALEQVWDLLINYNQ
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA