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The map label for this gene is gsiB [H]

Identifier: 158315357

GI number: 158315357

Start: 4223260

End: 4224840

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_3559

Alternate gene names: 158315357

Gene position: 4224840-4223260 (Counterclockwise)

Preceding gene: 158315359

Following gene: 158315356

Centisome position: 47.04

GC content: 72.93

Gene sequence:

>1581_bases
ATGAACGTTGCTCTCAACCGTCGCGCGTTCGGGCGTGCCGGCCTGCTCGCCGTCGGCGCCCTCACGGCGCCGGCACTGCT
CGCGGCCTGTGGCGACGACGCCCCCGCCGCACCAGGCGCGGGCACGGGCGCGGCGGGGGCGACAGCGGCCCCGCGCCGCG
GCGGGAAACTGCGGGCCGCGTTCGCGAGCAGTCCGGCCGACACCCTGGACATCGTGAAAGGCCACGACGTGCTGGGCTCG
GTGCGCGGCCTCGCGGTGTACGAACGCCTCGGCGACGCCCACCCGGACGGCTCGGTCTCCCCGCGCCTGTTCGAGAGCCT
GACGCCCAACGCCGACGCGACCGTCTGGACGCTGAAGCTGAAGCCGGGGATCACCTTCTCCGATGGCAGGCCGTTGACGA
CGGCCGACGTGCTGGCCAGCTTCGCGACGTTCACCGGCACCGAGAGCGGCGCCGCGATCGCCGCGTTCGACCCGAAGGAG
AGCAAGGCGACGGACGCGCGCACCGCGACCATCGCGCTGACCGCCCCCGTGTACGACCTGCCGGCCCGGGTGAGCGGCGT
CGTCCTGGTGATCATGCCGGAGGGCAAGCCGGCGTCTCAGCTCGGTGACGTCGTCGGCAGCGGGCCCTACGAGATCGCCT
CGTTCGTGCCCGGCCAGCGGACGGTCCTGCGCAGGCGCACCGACTACTGGGACGGCGACGCCCGGGGCTACCTGGACGCG
ATCGAGCTGGTGGCCGCGCCGGACGCGAAGTCGCGGCTGTCCGCGCTGCGGGCGGGCCAGGTGGACTGGGCCGACGACAT
CGCCTACCTGGACGCCTCGACCCTGCGGGAGGACCGCGCGATCACGATCCACCGCGGCGCGGCCGAGCAGGGCCTGGCCT
GGTTCCTGAACATGGCGGCGCCGCCGTTCGACGACGAGCGCGTCCGGCAGGCCCTGCGGTACTCGGTGGACCGCCAGAAG
CTCGTCGACACCACGCTGTTCGGATTCGGCTCCGTCGGCAACGACCTGTGGGGCAAGGGCCTGCCGAACTACAACGGCTC
GATCCCGCAGCGGCCGCACGACCCCGCGAAGGCGAAATCGCTCCTGCAGGAGGCCGGGGTGGCCACCCCGGCCAAGGCCA
CCCTGCTGACCTCGCCCATCGGCCCGGGTCTCGTCGAGGCGACCCAGCTCCTCGCCGACCAGGCCCGCGAGGTCGGCTTC
GACATCAAGGTCGAGGTCATCCCGCCGGACGTCTACTTCGCCCGGCCCGAGGAGTGGGCGAAGGCGTCGGGCGTGGCGTT
CGCCCAGGTCGGCGCGTTCACCGACATGGCCCCGCTGGTCTACCTGTCCGACGGCCCGTTCAACTTCGGCTGGCGCAAGC
CCGACTGGGACGCCGGGTTCGCCGACGGAGTGGGCGAGCTCGACGCCGCGAAGCGCAAAGCGACGTTCGACGGCCTGCAG
CAGCAGCTCTGGGATTCCGGCTCCGACCTCGTGTGGGGGTTCGCGCCGAGGCTCGTCGCCGCCGCGCCGTCCGTCGGGGG
CGTCGACTCCAGTCCCAACTTCGGCATCCCGGACCTGGTCTTCATCCACCGCACGGGGTGA

Upstream 100 bases:

>100_bases
CAGATACAACTAAGGACTTCCTTAAGCGAATGGTCTATGCATAAGGTGACCCTTAGCTGCCCGCTGGTTGGGCGGCACGG
GCCCCCGGGAGAAGACCTCG

Downstream 100 bases:

>100_bases
GCACTCCGCCGGCCGGGTCGCGAGCGGTGGCCGGCCGCGTGCCGTCGCCCGTCCGGCGGCTGGCCGGGGCCTTCGCCGTC
GTCCTCGGGGTGTCCGTCGT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNVALNRRAFGRAGLLAVGALTAPALLAACGDDAPAAPGAGTGAAGATAAPRRGGKLRAAFASSPADTLDIVKGHDVLGS
VRGLAVYERLGDAHPDGSVSPRLFESLTPNADATVWTLKLKPGITFSDGRPLTTADVLASFATFTGTESGAAIAAFDPKE
SKATDARTATIALTAPVYDLPARVSGVVLVIMPEGKPASQLGDVVGSGPYEIASFVPGQRTVLRRRTDYWDGDARGYLDA
IELVAAPDAKSRLSALRAGQVDWADDIAYLDASTLREDRAITIHRGAAEQGLAWFLNMAAPPFDDERVRQALRYSVDRQK
LVDTTLFGFGSVGNDLWGKGLPNYNGSIPQRPHDPAKAKSLLQEAGVATPAKATLLTSPIGPGLVEATQLLADQAREVGF
DIKVEVIPPDVYFARPEEWAKASGVAFAQVGAFTDMAPLVYLSDGPFNFGWRKPDWDAGFADGVGELDAAKRKATFDGLQ
QQLWDSGSDLVWGFAPRLVAAAPSVGGVDSSPNFGIPDLVFIHRTG

Sequences:

>Translated_526_residues
MNVALNRRAFGRAGLLAVGALTAPALLAACGDDAPAAPGAGTGAAGATAAPRRGGKLRAAFASSPADTLDIVKGHDVLGS
VRGLAVYERLGDAHPDGSVSPRLFESLTPNADATVWTLKLKPGITFSDGRPLTTADVLASFATFTGTESGAAIAAFDPKE
SKATDARTATIALTAPVYDLPARVSGVVLVIMPEGKPASQLGDVVGSGPYEIASFVPGQRTVLRRRTDYWDGDARGYLDA
IELVAAPDAKSRLSALRAGQVDWADDIAYLDASTLREDRAITIHRGAAEQGLAWFLNMAAPPFDDERVRQALRYSVDRQK
LVDTTLFGFGSVGNDLWGKGLPNYNGSIPQRPHDPAKAKSLLQEAGVATPAKATLLTSPIGPGLVEATQLLADQAREVGF
DIKVEVIPPDVYFARPEEWAKASGVAFAQVGAFTDMAPLVYLSDGPFNFGWRKPDWDAGFADGVGELDAAKRKATFDGLQ
QQLWDSGSDLVWGFAPRLVAAAPSVGGVDSSPNFGIPDLVFIHRTG
>Mature_526_residues
MNVALNRRAFGRAGLLAVGALTAPALLAACGDDAPAAPGAGTGAAGATAAPRRGGKLRAAFASSPADTLDIVKGHDVLGS
VRGLAVYERLGDAHPDGSVSPRLFESLTPNADATVWTLKLKPGITFSDGRPLTTADVLASFATFTGTESGAAIAAFDPKE
SKATDARTATIALTAPVYDLPARVSGVVLVIMPEGKPASQLGDVVGSGPYEIASFVPGQRTVLRRRTDYWDGDARGYLDA
IELVAAPDAKSRLSALRAGQVDWADDIAYLDASTLREDRAITIHRGAAEQGLAWFLNMAAPPFDDERVRQALRYSVDRQK
LVDTTLFGFGSVGNDLWGKGLPNYNGSIPQRPHDPAKAKSLLQEAGVATPAKATLLTSPIGPGLVEATQLLADQAREVGF
DIKVEVIPPDVYFARPEEWAKASGVAFAQVGAFTDMAPLVYLSDGPFNFGWRKPDWDAGFADGVGELDAAKRKATFDGLQ
QQLWDSGSDLVWGFAPRLVAAAPSVGGVDSSPNFGIPDLVFIHRTG

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=417, Percent_Identity=25.6594724220624, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789887, Length=461, Percent_Identity=23.2104121475054, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787495, Length=549, Percent_Identity=24.2258652094718, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789966, Length=311, Percent_Identity=23.7942122186495, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787762, Length=337, Percent_Identity=24.6290801186944, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55219; Mature: 55219

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVALNRRAFGRAGLLAVGALTAPALLAACGDDAPAAPGAGTGAAGATAAPRRGGKLRAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
FASSPADTLDIVKGHDVLGSVRGLAVYERLGDAHPDGSVSPRLFESLTPNADATVWTLKL
ECCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEEEEEE
KPGITFSDGRPLTTADVLASFATFTGTESGAAIAAFDPKESKATDARTATIALTAPVYDL
CCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHC
PARVSGVVLVIMPEGKPASQLGDVVGSGPYEIASFVPGQRTVLRRRTDYWDGDARGYLDA
CCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHH
IELVAAPDAKSRLSALRAGQVDWADDIAYLDASTLREDRAITIHRGAAEQGLAWFLNMAA
HHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCHHHHCCCCEEEEECCCCHHHHHHHHHCCC
PPFDDERVRQALRYSVDRQKLVDTTLFGFGSVGNDLWGKGLPNYNGSIPQRPHDPAKAKS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHH
LLQEAGVATPAKATLLTSPIGPGLVEATQLLADQAREVGFDIKVEVIPPDVYFARPEEWA
HHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECCHHHH
KASGVAFAQVGAFTDMAPLVYLSDGPFNFGWRKPDWDAGFADGVGELDAAKRKATFDGLQ
HHCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QQLWDSGSDLVWGFAPRLVAAAPSVGGVDSSPNFGIPDLVFIHRTG
HHHHCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MNVALNRRAFGRAGLLAVGALTAPALLAACGDDAPAAPGAGTGAAGATAAPRRGGKLRAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
FASSPADTLDIVKGHDVLGSVRGLAVYERLGDAHPDGSVSPRLFESLTPNADATVWTLKL
ECCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEEEEEE
KPGITFSDGRPLTTADVLASFATFTGTESGAAIAAFDPKESKATDARTATIALTAPVYDL
CCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHC
PARVSGVVLVIMPEGKPASQLGDVVGSGPYEIASFVPGQRTVLRRRTDYWDGDARGYLDA
CCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHH
IELVAAPDAKSRLSALRAGQVDWADDIAYLDASTLREDRAITIHRGAAEQGLAWFLNMAA
HHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCHHHHCCCCEEEEECCCCHHHHHHHHHCCC
PPFDDERVRQALRYSVDRQKLVDTTLFGFGSVGNDLWGKGLPNYNGSIPQRPHDPAKAKS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHH
LLQEAGVATPAKATLLTSPIGPGLVEATQLLADQAREVGFDIKVEVIPPDVYFARPEEWA
HHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEECCHHHH
KASGVAFAQVGAFTDMAPLVYLSDGPFNFGWRKPDWDAGFADGVGELDAAKRKATFDGLQ
HHCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QQLWDSGSDLVWGFAPRLVAAAPSVGGVDSSPNFGIPDLVFIHRTG
HHHHCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA