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The map label for this gene is metE [H]

Identifier: 158315339

GI number: 158315339

Start: 4198544

End: 4200844

Strand: Reverse

Name: metE [H]

Synonym: Franean1_3540

Alternate gene names: 158315339

Gene position: 4200844-4198544 (Counterclockwise)

Preceding gene: 158315345

Following gene: 158315338

Centisome position: 46.77

GC content: 73.79

Gene sequence:

>2301_bases
GTGGTGAGCACGACCGTCGCCGGCTACCCGCGGATCGGGCCGGCCCGCGAGCTGAAGACCACGACCGAGGCGTACTGGGC
CGACCGGCTCGGCGAGGACGAGCTGGTCTGGATCGCCCAGCAGCTGCGCGCCGACGTGTGGAAGGATCTCGCGGCCGCCG
GGCTGGACGCCATCCCGTCGAACACGTTCTCGTTCTATGACCAGGTCCTCGACACCGCCGTGCTCTTCGACGCCGTGCCG
GACCGTTTCCGCGGCCTGACCGGTGCGGGCGGCTCGCCCGCCACCCCGCTGGAGACGTACTTCGCGATGGCCCGCGGCGC
CGACGGGGTCGCGCCGCTGGAGATGACCAAGTGGTTCGACACCAACTACCACTACCTTGTGCCCGAGCTGGACCCGTCGA
CCCGGTTCCGCCTCGTCGGCGACAAGCCGCTGCGCGAGGTCCGGGAGGCCCGGGAGCTGGGCGTGGAGACCCGGCCGGTG
CTCGTCGGCCCGGTCACCTTCCTGCTGCTGGCGAAGGCCGCGGCTGGCGCGCCCGCGGGATTCCGGCCGCTGGACCTGCT
CGACGACCTGGTCGGGCAGTACGTGGAGCTGCTCGACGACCTCGCCTCCGACAGCGTCGCCTGGGTGCAGCTGGACGAGC
CGGCGCTCACCCGGGACCTGACCCCGGCCGAGCTCGCGGCGACCGCGCGCGCCTACGCGCGCCTCGGCGGGGCGACCACC
CGGCCGAGGATCCTGGTCTCGACCTTCTTCGGGGAGGCCGGCGAGGCCCTGCCCGTCCTACGGGACGCCCCGATCGACGG
CATCGGCCTCGATCTCGTCGCCGGGCCGGGGAACCTCGACGCGGTCGCCCGGGCCGGCGGCGTCGGGGCGAAGACGCTGT
TCGTCGGCGTCGTGAACGGCCACAACGTCTGGCGCGCCGACCTGCCGGCCGCGCTGGCCACCTGTGCCACGCTGAGCGGC
CTCGCCGCCGACGTCGTCGTCACGACGTCGTGCTCGCTGCTGCACGTACCGATCGACGTCGAGGCGGAGACGAACCTCGA
CCCGCGGCTGGCCGACCGGATGGCCTTCGCCCGGCAGAAGGTCGAGGAGGTCGTGCTGCTCGGCACCGCGCTGCGCGCGG
GCCGCGACGCCGTGGCCGCGGAGATCGCGGCCGCCGAGGCGCGCCGGCTCGCCGTCCCCGACCAGCTCGTCGACCCACGG
GTGCGCGAGCGGCTCGCGGCGCTGGGCGAGGGCTGGCCGACCCGCGGGGATCTCGAGCACCGGGTCGAGGCGCAGGCCGC
CGCGCTCGGGCTGCCGCCGCTGCCCACCACGACGATCGGCTCGTTCCCGCAGACGGCGGCGATCCGCGCGGCACGGGCCT
CCCGCCGCGCCGGCACCCTCGACGAGGCCGGGTACGTCCGGGCGATGAAGACCGAGATCGACCAGGTCGTCGCCCTGCAG
GAGGACATCGGACTCGACGTGCTCGTGCACGGCGAGCCAGAGCGCAACGACATGGTCCAGTACTTCGCCGAGCTGCTGGC
CGGCTACGCGGCCACCGAGCACGGCTGGGTGCAGTCCTACGGCACCCGCTACGTCCGCCCGCCGATCCTGTTCGGTGACG
TCTCCCGGCCCGAGCCGATGACCCTGCGGTGGGCCGCCTACGCGCAGTCCCGCACCAGCAGGCCGGTCAAGGGCATGCTC
ACCGGCCCGGTCACCATGCTCGCCTGGTCGTTCGTGCGCGACGACCAGCCGCTGGAGGTGACCGCCCGCCAGGTCGCCCT
GGCGCTGCGGGACGAGATCCACGGCCTGGAGGCCGCCGGCATCCGGATCATCCAGGTGGACGAGCCGGCGCTGCGCGAGC
TGCTCCCGCCGCGCCGGGCGCTGTGGGGGGCCTACCTCGACTGGGCGGTCGGCGCCTTCCGGCTGGCGACCTCGTCCGTG
GCGGCGAGCACCCAGATCCACACTCACATGTGCTACTCGGAGTTCGGCGACATCATCGGCTCCATCGACGACCTCGACGC
GGACGTCGCCAGCGTCGAGGCCGCCCGCTCCCGGATGGAGCTGGTGACCGACCTGCGGAAGGCCGGCTACCGGCGGGCCA
TCGGCCCGGGTGTCTACGACATCCACTCCCCCCGGGTGCCCACGGTGGACGAGATCGAGAAGTCGCTGCGGCTGGCGCTC
GCCGCGGTAGAACCCGCCCGGCTGTGGGCCAATCCCGACTGCGGGCTGAAGACCCGCAGTTTCACCGAGGTCGAGCCGGC
GCTGCGCAACATGGTCACCGCGACGCGCAGGGTCCGCGAATCCCTCCCGGACGGAGGCTGA

Upstream 100 bases:

>100_bases
TCGTGTCCTTCCCCTCGCGGGTCGCGGTCCGTGGCGCGTTTCCCGAGGGAAGGAAACCCATGAGCGACATCGCCGCACGT
CCGGCCGGACCGGTACTCCC

Downstream 100 bases:

>100_bases
TCCCGCCCGGCGACACGGAATCCGGCTCGTCGGGCCGCGGTCTGAGGGCCGGATGCACAACCGCCACGGTCAGGTGCCCG
GTCGGTGCGGATGCGAGAAT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 766; Mature: 766

Protein sequence:

>766_residues
MVSTTVAGYPRIGPARELKTTTEAYWADRLGEDELVWIAQQLRADVWKDLAAAGLDAIPSNTFSFYDQVLDTAVLFDAVP
DRFRGLTGAGGSPATPLETYFAMARGADGVAPLEMTKWFDTNYHYLVPELDPSTRFRLVGDKPLREVREARELGVETRPV
LVGPVTFLLLAKAAAGAPAGFRPLDLLDDLVGQYVELLDDLASDSVAWVQLDEPALTRDLTPAELAATARAYARLGGATT
RPRILVSTFFGEAGEALPVLRDAPIDGIGLDLVAGPGNLDAVARAGGVGAKTLFVGVVNGHNVWRADLPAALATCATLSG
LAADVVVTTSCSLLHVPIDVEAETNLDPRLADRMAFARQKVEEVVLLGTALRAGRDAVAAEIAAAEARRLAVPDQLVDPR
VRERLAALGEGWPTRGDLEHRVEAQAAALGLPPLPTTTIGSFPQTAAIRAARASRRAGTLDEAGYVRAMKTEIDQVVALQ
EDIGLDVLVHGEPERNDMVQYFAELLAGYAATEHGWVQSYGTRYVRPPILFGDVSRPEPMTLRWAAYAQSRTSRPVKGML
TGPVTMLAWSFVRDDQPLEVTARQVALALRDEIHGLEAAGIRIIQVDEPALRELLPPRRALWGAYLDWAVGAFRLATSSV
AASTQIHTHMCYSEFGDIIGSIDDLDADVASVEAARSRMELVTDLRKAGYRRAIGPGVYDIHSPRVPTVDEIEKSLRLAL
AAVEPARLWANPDCGLKTRSFTEVEPALRNMVTATRRVRESLPDGG

Sequences:

>Translated_766_residues
MVSTTVAGYPRIGPARELKTTTEAYWADRLGEDELVWIAQQLRADVWKDLAAAGLDAIPSNTFSFYDQVLDTAVLFDAVP
DRFRGLTGAGGSPATPLETYFAMARGADGVAPLEMTKWFDTNYHYLVPELDPSTRFRLVGDKPLREVREARELGVETRPV
LVGPVTFLLLAKAAAGAPAGFRPLDLLDDLVGQYVELLDDLASDSVAWVQLDEPALTRDLTPAELAATARAYARLGGATT
RPRILVSTFFGEAGEALPVLRDAPIDGIGLDLVAGPGNLDAVARAGGVGAKTLFVGVVNGHNVWRADLPAALATCATLSG
LAADVVVTTSCSLLHVPIDVEAETNLDPRLADRMAFARQKVEEVVLLGTALRAGRDAVAAEIAAAEARRLAVPDQLVDPR
VRERLAALGEGWPTRGDLEHRVEAQAAALGLPPLPTTTIGSFPQTAAIRAARASRRAGTLDEAGYVRAMKTEIDQVVALQ
EDIGLDVLVHGEPERNDMVQYFAELLAGYAATEHGWVQSYGTRYVRPPILFGDVSRPEPMTLRWAAYAQSRTSRPVKGML
TGPVTMLAWSFVRDDQPLEVTARQVALALRDEIHGLEAAGIRIIQVDEPALRELLPPRRALWGAYLDWAVGAFRLATSSV
AASTQIHTHMCYSEFGDIIGSIDDLDADVASVEAARSRMELVTDLRKAGYRRAIGPGVYDIHSPRVPTVDEIEKSLRLAL
AAVEPARLWANPDCGLKTRSFTEVEPALRNMVTATRRVRESLPDGG
>Mature_766_residues
MVSTTVAGYPRIGPARELKTTTEAYWADRLGEDELVWIAQQLRADVWKDLAAAGLDAIPSNTFSFYDQVLDTAVLFDAVP
DRFRGLTGAGGSPATPLETYFAMARGADGVAPLEMTKWFDTNYHYLVPELDPSTRFRLVGDKPLREVREARELGVETRPV
LVGPVTFLLLAKAAAGAPAGFRPLDLLDDLVGQYVELLDDLASDSVAWVQLDEPALTRDLTPAELAATARAYARLGGATT
RPRILVSTFFGEAGEALPVLRDAPIDGIGLDLVAGPGNLDAVARAGGVGAKTLFVGVVNGHNVWRADLPAALATCATLSG
LAADVVVTTSCSLLHVPIDVEAETNLDPRLADRMAFARQKVEEVVLLGTALRAGRDAVAAEIAAAEARRLAVPDQLVDPR
VRERLAALGEGWPTRGDLEHRVEAQAAALGLPPLPTTTIGSFPQTAAIRAARASRRAGTLDEAGYVRAMKTEIDQVVALQ
EDIGLDVLVHGEPERNDMVQYFAELLAGYAATEHGWVQSYGTRYVRPPILFGDVSRPEPMTLRWAAYAQSRTSRPVKGML
TGPVTMLAWSFVRDDQPLEVTARQVALALRDEIHGLEAAGIRIIQVDEPALRELLPPRRALWGAYLDWAVGAFRLATSSV
AASTQIHTHMCYSEFGDIIGSIDDLDADVASVEAARSRMELVTDLRKAGYRRAIGPGVYDIHSPRVPTVDEIEKSLRLAL
AAVEPARLWANPDCGLKTRSFTEVEPALRNMVTATRRVRESLPDGG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=758, Percent_Identity=47.0976253298153, Blast_Score=645, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=788, Percent_Identity=45.3045685279188, Blast_Score=659, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 82698; Mature: 82698

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTTVAGYPRIGPARELKTTTEAYWADRLGEDELVWIAQQLRADVWKDLAAAGLDAIPS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTFSFYDQVLDTAVLFDAVPDRFRGLTGAGGSPATPLETYFAMARGADGVAPLEMTKWFD
CHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCC
TNYHYLVPELDPSTRFRLVGDKPLREVREARELGVETRPVLVGPVTFLLLAKAAAGAPAG
CCCEEEECCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCC
FRPLDLLDDLVGQYVELLDDLASDSVAWVQLDEPALTRDLTPAELAATARAYARLGGATT
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCC
RPRILVSTFFGEAGEALPVLRDAPIDGIGLDLVAGPGNLDAVARAGGVGAKTLFVGVVNG
CCEEEEEEHHCCCCCCCCHHHCCCCCCCCEEEEECCCCHHHHHHCCCCCCEEEEEEEECC
HNVWRADLPAALATCATLSGLAADVVVTTSCSLLHVPIDVEAETNLDPRLADRMAFARQK
CCEEHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHH
VEEVVLLGTALRAGRDAVAAEIAAAEARRLAVPDQLVDPRVRERLAALGEGWPTRGDLEH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCCCCCCHHH
RVEAQAAALGLPPLPTTTIGSFPQTAAIRAARASRRAGTLDEAGYVRAMKTEIDQVVALQ
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EDIGLDVLVHGEPERNDMVQYFAELLAGYAATEHGWVQSYGTRYVRPPILFGDVSRPEPM
HCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEECCCCCCCCC
TLRWAAYAQSRTSRPVKGMLTGPVTMLAWSFVRDDQPLEVTARQVALALRDEIHGLEAAG
EEEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
IRIIQVDEPALRELLPPRRALWGAYLDWAVGAFRLATSSVAASTQIHTHMCYSEFGDIIG
EEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SIDDLDADVASVEAARSRMELVTDLRKAGYRRAIGPGVYDIHSPRVPTVDEIEKSLRLAL
CHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCCCCCHHHHHHHHHHHH
AAVEPARLWANPDCGLKTRSFTEVEPALRNMVTATRRVRESLPDGG
HHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVSTTVAGYPRIGPARELKTTTEAYWADRLGEDELVWIAQQLRADVWKDLAAAGLDAIPS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTFSFYDQVLDTAVLFDAVPDRFRGLTGAGGSPATPLETYFAMARGADGVAPLEMTKWFD
CHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCC
TNYHYLVPELDPSTRFRLVGDKPLREVREARELGVETRPVLVGPVTFLLLAKAAAGAPAG
CCCEEEECCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCC
FRPLDLLDDLVGQYVELLDDLASDSVAWVQLDEPALTRDLTPAELAATARAYARLGGATT
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCCC
RPRILVSTFFGEAGEALPVLRDAPIDGIGLDLVAGPGNLDAVARAGGVGAKTLFVGVVNG
CCEEEEEEHHCCCCCCCCHHHCCCCCCCCEEEEECCCCHHHHHHCCCCCCEEEEEEEECC
HNVWRADLPAALATCATLSGLAADVVVTTSCSLLHVPIDVEAETNLDPRLADRMAFARQK
CCEEHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHH
VEEVVLLGTALRAGRDAVAAEIAAAEARRLAVPDQLVDPRVRERLAALGEGWPTRGDLEH
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCCCCCCHHH
RVEAQAAALGLPPLPTTTIGSFPQTAAIRAARASRRAGTLDEAGYVRAMKTEIDQVVALQ
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EDIGLDVLVHGEPERNDMVQYFAELLAGYAATEHGWVQSYGTRYVRPPILFGDVSRPEPM
HCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEECCCCCCCCC
TLRWAAYAQSRTSRPVKGMLTGPVTMLAWSFVRDDQPLEVTARQVALALRDEIHGLEAAG
EEEHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
IRIIQVDEPALRELLPPRRALWGAYLDWAVGAFRLATSSVAASTQIHTHMCYSEFGDIIG
EEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SIDDLDADVASVEAARSRMELVTDLRKAGYRRAIGPGVYDIHSPRVPTVDEIEKSLRLAL
CHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEECCCCCCCCHHHHHHHHHHHH
AAVEPARLWANPDCGLKTRSFTEVEPALRNMVTATRRVRESLPDGG
HHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]