Click here to switch to the map view.

The map label for this gene is prpB2 [H]

Identifier: 158315296

GI number: 158315296

Start: 4160017

End: 4160961

Strand: Direct

Name: prpB2 [H]

Synonym: Franean1_3496

Alternate gene names: 158315296

Gene position: 4160017-4160961 (Clockwise)

Preceding gene: 158315295

Following gene: 158315299

Centisome position: 46.31

GC content: 69.84

Gene sequence:

>945_bases
GTGCCCGGTGCCCGGCGGCCGAATCCGCCGGATTTCGGAAAGGAACCCGCAGTGCTGCACGCGAAGAAGCCGGCCCATTC
CCGTCGCGCCGCGTTCCGGGAGGCGCTGCGTTCCGGTCGCCTGCTGCGTTTCCCTGGCGCGTTCAACCCGCTGACCGCTG
TTCTCATCACCGAGCTCGGCTTCGACGGCGTCTACGTCTCCGGTGCGGTGCTCTCCGCCGATCTCGCCCTGCCCGACATC
GGTCTGACCACGTTGACCGAGGTCGCCGGGCGGGCCGGACAGATCGCCCGGGTCACCGACCTGCCGGCCCTCGTGGACGC
CGACACTGGCTTCGGCGAGCCGATGAACGTCGCCCGCACCATCCAGATCCTCGAGGACGCCGGGCTCGCCGGCTGCCACC
TCGAGGACCAGGTCAACCCGAAGCGGTGCGGTCATCTCGATGGCAAGTCCGTGGTGGGCACCGAGGAGATGGTCCGCCGG
ATCCACGCCGCGGTCACCGCCCGCCGTGACGACAACTTCGTCATCTGCGCCCGCACCGACGCCCGCGCCGGCGAGGGCCT
GGACGCCGCCACCGAGCGCGCCCGCGCCTACGCCGACGCCGGCGCGGACATGATCTTCCCGGAGGCGATGGCCGACCTCG
CCGAGTTCGAGGCGATGCGCAGCGCGGTCGACGTGCCGATCCTGGCGAACATGACCGAGTTCGGGAAAAGCGAGCTTCTC
ACCACCGCGGCGCTGGAATCCGCCGGCGTGAACCTCGTCATCTACCCGGTCACCCTGCTGCGCCTCGCGATGGGCGCCGT
CGAGGACGGGCTGCGCCAGCTCCTGGCGGACGGCACACAGGCCGGTCTCGTCGACCGGATGCAGACCCGCTCCCGCCTCT
ACGAGCTCCTCGACTATTCCGCCTACAACACCTTCGACACCAACACCTTCAACTTCCGGATCTGA

Upstream 100 bases:

>100_bases
GCCTCACCCTCACTCCCGAGCCCGCACTCACCACCGGGAGCACCCAGGGGATCTTCTGACCGCCCCGCCCCGCCCCGCGA
CAGCAGGCGGCCGGTGCCCG

Downstream 100 bases:

>100_bases
ACATTCCGGTCTTCTCCCTGGAAGGACGTCTGCCATAGCCACCGTCGAATCCTCGGTGCGCAAGGCTCTGGCCGGGCGCG
GAGGAGCGCAGCGTCCGCTC

Product: methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase 2 [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPGARRPNPPDFGKEPAVLHAKKPAHSRRAAFREALRSGRLLRFPGAFNPLTAVLITELGFDGVYVSGAVLSADLALPDI
GLTTLTEVAGRAGQIARVTDLPALVDADTGFGEPMNVARTIQILEDAGLAGCHLEDQVNPKRCGHLDGKSVVGTEEMVRR
IHAAVTARRDDNFVICARTDARAGEGLDAATERARAYADAGADMIFPEAMADLAEFEAMRSAVDVPILANMTEFGKSELL
TTAALESAGVNLVIYPVTLLRLAMGAVEDGLRQLLADGTQAGLVDRMQTRSRLYELLDYSAYNTFDTNTFNFRI

Sequences:

>Translated_314_residues
MPGARRPNPPDFGKEPAVLHAKKPAHSRRAAFREALRSGRLLRFPGAFNPLTAVLITELGFDGVYVSGAVLSADLALPDI
GLTTLTEVAGRAGQIARVTDLPALVDADTGFGEPMNVARTIQILEDAGLAGCHLEDQVNPKRCGHLDGKSVVGTEEMVRR
IHAAVTARRDDNFVICARTDARAGEGLDAATERARAYADAGADMIFPEAMADLAEFEAMRSAVDVPILANMTEFGKSELL
TTAALESAGVNLVIYPVTLLRLAMGAVEDGLRQLLADGTQAGLVDRMQTRSRLYELLDYSAYNTFDTNTFNFRI
>Mature_313_residues
PGARRPNPPDFGKEPAVLHAKKPAHSRRAAFREALRSGRLLRFPGAFNPLTAVLITELGFDGVYVSGAVLSADLALPDIG
LTTLTEVAGRAGQIARVTDLPALVDADTGFGEPMNVARTIQILEDAGLAGCHLEDQVNPKRCGHLDGKSVVGTEEMVRRI
HAAVTARRDDNFVICARTDARAGEGLDAATERARAYADAGADMIFPEAMADLAEFEAMRSAVDVPILANMTEFGKSELLT
TAALESAGVNLVIYPVTLLRLAMGAVEDGLRQLLADGTQAGLVDRMQTRSRLYELLDYSAYNTFDTNTFNFRI

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=287, Percent_Identity=48.4320557491289, Blast_Score=247, Evalue=6e-67,
Organism=Escherichia coli, GI1790445, Length=170, Percent_Identity=31.7647058823529, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 33744; Mature: 33613

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGARRPNPPDFGKEPAVLHAKKPAHSRRAAFREALRSGRLLRFPGAFNPLTAVLITELG
CCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHC
FDGVYVSGAVLSADLALPDIGLTTLTEVAGRAGQIARVTDLPALVDADTGFGEPMNVART
CCCEEECCHHEECCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHEECCCCCCCCHHHHHH
IQILEDAGLAGCHLEDQVNPKRCGHLDGKSVVGTEEMVRRIHAAVTARRDDNFVICARTD
HHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
ARAGEGLDAATERARAYADAGADMIFPEAMADLAEFEAMRSAVDVPILANMTEFGKSELL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHH
TTAALESAGVNLVIYPVTLLRLAMGAVEDGLRQLLADGTQAGLVDRMQTRSRLYELLDYS
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AYNTFDTNTFNFRI
CCCCCCCCEEEEEC
>Mature Secondary Structure 
PGARRPNPPDFGKEPAVLHAKKPAHSRRAAFREALRSGRLLRFPGAFNPLTAVLITELG
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHC
FDGVYVSGAVLSADLALPDIGLTTLTEVAGRAGQIARVTDLPALVDADTGFGEPMNVART
CCCEEECCHHEECCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHEECCCCCCCCHHHHHH
IQILEDAGLAGCHLEDQVNPKRCGHLDGKSVVGTEEMVRRIHAAVTARRDDNFVICARTD
HHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECC
ARAGEGLDAATERARAYADAGADMIFPEAMADLAEFEAMRSAVDVPILANMTEFGKSELL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHH
TTAALESAGVNLVIYPVTLLRLAMGAVEDGLRQLLADGTQAGLVDRMQTRSRLYELLDYS
HHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AYNTFDTNTFNFRI
CCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]