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The map label for this gene is prpD2 [H]

Identifier: 158315295

GI number: 158315295

Start: 4158467

End: 4159975

Strand: Direct

Name: prpD2 [H]

Synonym: Franean1_3495

Alternate gene names: 158315295

Gene position: 4158467-4159975 (Clockwise)

Preceding gene: 158315293

Following gene: 158315296

Centisome position: 46.3

GC content: 71.77

Gene sequence:

>1509_bases
ATGATCGACCACAAGGTCCGCGTGTATCGGAGCGCCGAGCGGCTGGCCCGCGAGGACCAGCTCGCCTGGAAGATCGCCGC
GGTCGCCACCGACCCGGTCGAGGTCACCGCCGACGTGACCGACATGGTGATCAACCGGGTCATCGACAACGCCGGGGTGG
CCGCCGCGTCGCTGACCCGAAAGCCGGTGGCCGCCGCCCGCGACCAGGCGCTGCGGCACATCCCCGCCCCCGGGCGTGAC
GGCGCTACCGTGTTCGGCACGCCGGCCGGCGTCCGCGTCTCCCCGGAGTGGGCAGCCTGGGCGAACGGCGTCGCCGTGCG
CGAGCTGGACTTCCACGACACCTACCTGGCGGCGGACTACTCCCACCCCGGGGACAACATCCCGCCGGTGCTGGCCGTCG
CCCAGCATCTGGGGCTGGACGGTGCCGCCCTGCTGCGCGGGATCGCCACCGGCTACGAGATCCAGGTCGCGCTCGTCCGT
GGGATCTGCCTGCACCGGCACAAGATCGACCACATCGCGCACCTCGGCCCGTCCGCCGCCGCCGGCATCGGCGCCCTGCT
CGGCCTGCCGACCGAGACCGTCCACCAGGCTGTCGGACAGGCCCTGCACACGACCACAACAACCCGGCAGTCACGCAAGG
GCGAGATCTCGAGCTGGAAGGCGTACGCACCGGCGTTCGCCGGCAAGCTCGCCGTCGAGGCGGTGGACCGGGCCATGCGC
GGTGAGGGCGCGCCGTCACCGGCCTACGAGGGTGAGGACGGGTTCATCGCCTGGCTGCTCGACGGGCCCGGCGGGCAGTA
CGTCGTCTCGCTGCCCGCGGCCGGGGAGGCCAAGCGGGGGATCCTCGAGACCTACACCAAGGAGCACTCCGCCGAGTACC
AGAGCCAGGCGCTGATCGACCTGGCCCGCCGCCTCGGCCCGCGGATCGGGGACTTTGCCCGAGTCCGCTCCATTGCGATC
CACACCAGCCATCACACCCACTACGTGATCGGCTCCGGGGCGAACGACCCGCAGAAGTACGACCCGAAGGCCAGCAGGGA
GACCCTCGACCACTCGATTCCCTACATCTTCGCGGTCGCGTTGCAGGACGGCGACTGGCACCACGAGCGCTCCTACGCCC
CCGAGCGGGCGACGCGGCCGGACACCGTCGCCCTCTGGCGGAAGATCACCACGCTGGAGGACAAGGAGTGGACGCGGCGT
TACCACGCCACCGACCCCGCCGAGAAGGCGTTCGGTGGCCGGGTCGTCGTCGAGCTCGACGACGACACCGTGCTCACCGA
CGAGATCGCCGTCGCCGACGCGCATCCGCTCGGCGCCCGTCCGTTCGGCCGGGACGAGTACGTCGGCAAGTTCCGCCGCC
TCGCCGAGGGCGTCATCCCCGGCCCCGAGCAGGACAGGTTCCTCGACACCGCCGCCCGCCTGCCCGAACTGACCCCGGAC
GAACTCGCCGGCCTCACCCTCACTCCCGAGCCCGCACTCACCACCGGGAGCACCCAGGGGATCTTCTGA

Upstream 100 bases:

>100_bases
ACGCATGGTCGACGCGGGCCTGTTCGGCAGGAAGTCCGGGCACGGCTTCCACCCCTCTCTATCCCGGTTCGGCGGACAAC
TCGGAAGAAGGAGTGAACGC

Downstream 100 bases:

>100_bases
CCGCCCCGCCCCGCCCCGCGACAGCAGGCGGCCGGTGCCCGGTGCCCGGTGCCCGGCGGCCGAATCCGCCGGATTTCGGA
AAGGAACCCGCAGTGCTGCA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MIDHKVRVYRSAERLAREDQLAWKIAAVATDPVEVTADVTDMVINRVIDNAGVAAASLTRKPVAAARDQALRHIPAPGRD
GATVFGTPAGVRVSPEWAAWANGVAVRELDFHDTYLAADYSHPGDNIPPVLAVAQHLGLDGAALLRGIATGYEIQVALVR
GICLHRHKIDHIAHLGPSAAAGIGALLGLPTETVHQAVGQALHTTTTTRQSRKGEISSWKAYAPAFAGKLAVEAVDRAMR
GEGAPSPAYEGEDGFIAWLLDGPGGQYVVSLPAAGEAKRGILETYTKEHSAEYQSQALIDLARRLGPRIGDFARVRSIAI
HTSHHTHYVIGSGANDPQKYDPKASRETLDHSIPYIFAVALQDGDWHHERSYAPERATRPDTVALWRKITTLEDKEWTRR
YHATDPAEKAFGGRVVVELDDDTVLTDEIAVADAHPLGARPFGRDEYVGKFRRLAEGVIPGPEQDRFLDTAARLPELTPD
ELAGLTLTPEPALTTGSTQGIF

Sequences:

>Translated_502_residues
MIDHKVRVYRSAERLAREDQLAWKIAAVATDPVEVTADVTDMVINRVIDNAGVAAASLTRKPVAAARDQALRHIPAPGRD
GATVFGTPAGVRVSPEWAAWANGVAVRELDFHDTYLAADYSHPGDNIPPVLAVAQHLGLDGAALLRGIATGYEIQVALVR
GICLHRHKIDHIAHLGPSAAAGIGALLGLPTETVHQAVGQALHTTTTTRQSRKGEISSWKAYAPAFAGKLAVEAVDRAMR
GEGAPSPAYEGEDGFIAWLLDGPGGQYVVSLPAAGEAKRGILETYTKEHSAEYQSQALIDLARRLGPRIGDFARVRSIAI
HTSHHTHYVIGSGANDPQKYDPKASRETLDHSIPYIFAVALQDGDWHHERSYAPERATRPDTVALWRKITTLEDKEWTRR
YHATDPAEKAFGGRVVVELDDDTVLTDEIAVADAHPLGARPFGRDEYVGKFRRLAEGVIPGPEQDRFLDTAARLPELTPD
ELAGLTLTPEPALTTGSTQGIF
>Mature_502_residues
MIDHKVRVYRSAERLAREDQLAWKIAAVATDPVEVTADVTDMVINRVIDNAGVAAASLTRKPVAAARDQALRHIPAPGRD
GATVFGTPAGVRVSPEWAAWANGVAVRELDFHDTYLAADYSHPGDNIPPVLAVAQHLGLDGAALLRGIATGYEIQVALVR
GICLHRHKIDHIAHLGPSAAAGIGALLGLPTETVHQAVGQALHTTTTTRQSRKGEISSWKAYAPAFAGKLAVEAVDRAMR
GEGAPSPAYEGEDGFIAWLLDGPGGQYVVSLPAAGEAKRGILETYTKEHSAEYQSQALIDLARRLGPRIGDFARVRSIAI
HTSHHTHYVIGSGANDPQKYDPKASRETLDHSIPYIFAVALQDGDWHHERSYAPERATRPDTVALWRKITTLEDKEWTRR
YHATDPAEKAFGGRVVVELDDDTVLTDEIAVADAHPLGARPFGRDEYVGKFRRLAEGVIPGPEQDRFLDTAARLPELTPD
ELAGLTLTPEPALTTGSTQGIF

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=451, Percent_Identity=27.0509977827051, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6325258, Length=444, Percent_Identity=27.2522522522523, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 54257; Mature: 54257

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDHKVRVYRSAERLAREDQLAWKIAAVATDPVEVTADVTDMVINRVIDNAGVAAASLTR
CCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEHHHHHHHHHHHHHCCCCHHHHHHH
KPVAAARDQALRHIPAPGRDGATVFGTPAGVRVSPEWAAWANGVAVRELDFHDTYLAADY
CCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEECCCHHHHHCCEEEEEECCCCCEEEECC
SHPGDNIPPVLAVAQHLGLDGAALLRGIATGYEIQVALVRGICLHRHKIDHIAHLGPSAA
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHH
AGIGALLGLPTETVHQAVGQALHTTTTTRQSRKGEISSWKAYAPAFAGKLAVEAVDRAMR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GEGAPSPAYEGEDGFIAWLLDGPGGQYVVSLPAAGEAKRGILETYTKEHSAEYQSQALID
CCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LARRLGPRIGDFARVRSIAIHTSHHTHYVIGSGANDPQKYDPKASRETLDHSIPYIFAVA
HHHHHCCCCCHHHHHHEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEE
LQDGDWHHERSYAPERATRPDTVALWRKITTLEDKEWTRRYHATDPAEKAFGGRVVVELD
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCEEEEEEC
DDTVLTDEIAVADAHPLGARPFGRDEYVGKFRRLAEGVIPGPEQDRFLDTAARLPELTPD
CCEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHH
ELAGLTLTPEPALTTGSTQGIF
HHCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDHKVRVYRSAERLAREDQLAWKIAAVATDPVEVTADVTDMVINRVIDNAGVAAASLTR
CCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEHHHHHHHHHHHHHCCCCHHHHHHH
KPVAAARDQALRHIPAPGRDGATVFGTPAGVRVSPEWAAWANGVAVRELDFHDTYLAADY
CCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEECCCHHHHHCCEEEEEECCCCCEEEECC
SHPGDNIPPVLAVAQHLGLDGAALLRGIATGYEIQVALVRGICLHRHKIDHIAHLGPSAA
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHH
AGIGALLGLPTETVHQAVGQALHTTTTTRQSRKGEISSWKAYAPAFAGKLAVEAVDRAMR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GEGAPSPAYEGEDGFIAWLLDGPGGQYVVSLPAAGEAKRGILETYTKEHSAEYQSQALID
CCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LARRLGPRIGDFARVRSIAIHTSHHTHYVIGSGANDPQKYDPKASRETLDHSIPYIFAVA
HHHHHCCCCCHHHHHHEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEE
LQDGDWHHERSYAPERATRPDTVALWRKITTLEDKEWTRRYHATDPAEKAFGGRVVVELD
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCEEEEEEC
DDTVLTDEIAVADAHPLGARPFGRDEYVGKFRRLAEGVIPGPEQDRFLDTAARLPELTPD
CCEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHH
ELAGLTLTPEPALTTGSTQGIF
HHCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]