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The map label for this gene is lip2 [H]

Identifier: 158315258

GI number: 158315258

Start: 4087478

End: 4088398

Strand: Direct

Name: lip2 [H]

Synonym: Franean1_3458

Alternate gene names: 158315258

Gene position: 4087478-4088398 (Clockwise)

Preceding gene: 158315257

Following gene: 158315266

Centisome position: 45.51

GC content: 72.1

Gene sequence:

>921_bases
ATGACAAGGACGCCGACGCCGGCGCCGAATGCGGGAACCATCACCGACCTGGAGGCCGCGGTCAGGCAGGAGCGCGCCGG
GCGGGTGGCGATTCATGCCGACGAGCTGCCGCCGATCCCGGGGCTCACCATCACGGACCATCTTGTTCCCGGCTCCGATG
ACGACGTGAAGGTACGGGTGCGTGTTTACCGCCCCGAAGGCCGGCCCTCCCCGCTGCCGCTGGCCGTGTACATGCACGGG
GGCGCCTTCGTGCTCGGCAGCCTCGACATGGTTCATCAGGGCGTCGCCCAACTGGCTCTCGGTACCGGGGCGGTGATCGT
GTCGGTCGGGTACCGTCTCGCCCCCGAGCATCCTTTCCCCGCCGGCCTCAACGACTGCTACAGCGCCCTGCGATGGGCCT
CGGCGCATGCCGAGGAGCTGGGCGCCGACCCGGCCCGGATCGGACTGGCCGGGGTCAGCGCGGGTGCGACCCTGGCACTG
GGGGTGGCCCTGCTGACCCGCGACCGCGGCGGGCCGGCGGTACGTTTCCAGAGTGTGGTGATGCCCACCACGGACGACCG
GCTGGAGACACCTTCCATGCGGGACGCCGTCGACACTCCGGTCTGGAACCGCCCGCTCGCCGTCCAGTGCTGGCGCCTCT
ACCTCGGCGAGGGCGTGACCGAGGCCGACCAGTACGCGGCTCCCGCCCGGGCCGACGACCTCACCGGGCTGCCCCCGACC
TACCTCGCCGTCGGCGGCGAGGACCCGCTGCGCGATGAGGGGATCATTTTCGCGCTGCGGCTCATGCAGGCGGGGGTGCC
GGTTGATCTGCACGCCTACGCGGGCGCGGCGCACGGTTTCCGTGACCTGGACATCGACACCGCGCGGCGCGCCCAGGAGG
AGCAGATCTGGGCCCTACGGCGCGGCCTGGCCGCCGACTGA

Upstream 100 bases:

>100_bases
TCGACAGCGACGCGTGTCCCATGGTGGACGTCAGTTGATTCTGTCGCCGGGCCGGCCCATCCCCGATGCGGATCGAAAAG
CAGAGACCGAGGAGCGCGGA

Downstream 100 bases:

>100_bases
CCGAACTACCGGGCCGGCCTGAACTACCGGGCCGGCGTAGCGCCCGATCCAGCCGGGCTGGGTCGGGCGCTACGCCGCGC
CTAGGGGCCGCACTGTTCTG

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTRTPTPAPNAGTITDLEAAVRQERAGRVAIHADELPPIPGLTITDHLVPGSDDDVKVRVRVYRPEGRPSPLPLAVYMHG
GAFVLGSLDMVHQGVAQLALGTGAVIVSVGYRLAPEHPFPAGLNDCYSALRWASAHAEELGADPARIGLAGVSAGATLAL
GVALLTRDRGGPAVRFQSVVMPTTDDRLETPSMRDAVDTPVWNRPLAVQCWRLYLGEGVTEADQYAAPARADDLTGLPPT
YLAVGGEDPLRDEGIIFALRLMQAGVPVDLHAYAGAAHGFRDLDIDTARRAQEEQIWALRRGLAAD

Sequences:

>Translated_306_residues
MTRTPTPAPNAGTITDLEAAVRQERAGRVAIHADELPPIPGLTITDHLVPGSDDDVKVRVRVYRPEGRPSPLPLAVYMHG
GAFVLGSLDMVHQGVAQLALGTGAVIVSVGYRLAPEHPFPAGLNDCYSALRWASAHAEELGADPARIGLAGVSAGATLAL
GVALLTRDRGGPAVRFQSVVMPTTDDRLETPSMRDAVDTPVWNRPLAVQCWRLYLGEGVTEADQYAAPARADDLTGLPPT
YLAVGGEDPLRDEGIIFALRLMQAGVPVDLHAYAGAAHGFRDLDIDTARRAQEEQIWALRRGLAAD
>Mature_305_residues
TRTPTPAPNAGTITDLEAAVRQERAGRVAIHADELPPIPGLTITDHLVPGSDDDVKVRVRVYRPEGRPSPLPLAVYMHGG
AFVLGSLDMVHQGVAQLALGTGAVIVSVGYRLAPEHPFPAGLNDCYSALRWASAHAEELGADPARIGLAGVSAGATLALG
VALLTRDRGGPAVRFQSVVMPTTDDRLETPSMRDAVDTPVWNRPLAVQCWRLYLGEGVTEADQYAAPARADDLTGLPPTY
LAVGGEDPLRDEGIIFALRLMQAGVPVDLHAYAGAAHGFRDLDIDTARRAQEEQIWALRRGLAAD

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=361, Percent_Identity=27.1468144044321, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI157041239, Length=315, Percent_Identity=26.3492063492064, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI61966717, Length=281, Percent_Identity=27.0462633451957, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI21328446, Length=101, Percent_Identity=42.5742574257426, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1786682, Length=282, Percent_Identity=27.6595744680851, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=106, Percent_Identity=35.8490566037736, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI72001146, Length=140, Percent_Identity=33.5714285714286, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71996133, Length=106, Percent_Identity=34.9056603773585, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24656084, Length=102, Percent_Identity=40.1960784313725, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656076, Length=102, Percent_Identity=40.1960784313725, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130169, Length=102, Percent_Identity=40.1960784313725, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 32534; Mature: 32403

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTPTPAPNAGTITDLEAAVRQERAGRVAIHADELPPIPGLTITDHLVPGSDDDVKVRV
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCCCCCEEEEE
RVYRPEGRPSPLPLAVYMHGGAFVLGSLDMVHQGVAQLALGTGAVIVSVGYRLAPEHPFP
EEECCCCCCCCCCEEEEEECCEEEECCHHHHHHHHHHHHHCCCEEEEEECCEECCCCCCC
AGLNDCYSALRWASAHAEELGADPARIGLAGVSAGATLALGVALLTRDRGGPAVRFQSVV
CCHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHEECCCCCCEEEEEEEE
MPTTDDRLETPSMRDAVDTPVWNRPLAVQCWRLYLGEGVTEADQYAAPARADDLTGLPPT
CCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCC
YLAVGGEDPLRDEGIIFALRLMQAGVPVDLHAYAGAAHGFRDLDIDTARRAQEEQIWALR
EEEECCCCCCCCCCHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
RGLAAD
HCCCCC
>Mature Secondary Structure 
TRTPTPAPNAGTITDLEAAVRQERAGRVAIHADELPPIPGLTITDHLVPGSDDDVKVRV
CCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCCCCCCEEEEE
RVYRPEGRPSPLPLAVYMHGGAFVLGSLDMVHQGVAQLALGTGAVIVSVGYRLAPEHPFP
EEECCCCCCCCCCEEEEEECCEEEECCHHHHHHHHHHHHHCCCEEEEEECCEECCCCCCC
AGLNDCYSALRWASAHAEELGADPARIGLAGVSAGATLALGVALLTRDRGGPAVRFQSVV
CCHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHEECCCCCCEEEEEEEE
MPTTDDRLETPSMRDAVDTPVWNRPLAVQCWRLYLGEGVTEADQYAAPARADDLTGLPPT
CCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCC
YLAVGGEDPLRDEGIIFALRLMQAGVPVDLHAYAGAAHGFRDLDIDTARRAQEEQIWALR
EEEECCCCCCCCCCHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
RGLAAD
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]