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The map label for this gene is hupB [H]

Identifier: 158315204

GI number: 158315204

Start: 4035225

End: 4036946

Strand: Direct

Name: hupB [H]

Synonym: Franean1_3404

Alternate gene names: 158315204

Gene position: 4035225-4036946 (Clockwise)

Preceding gene: 158315203

Following gene: 158315205

Centisome position: 44.93

GC content: 70.15

Gene sequence:

>1722_bases
ATGAGTCGGCTTGTCATCGACCCGGTGTCGCGGATCGAGGGCCACCTGCGGGTCGAGGTGCAGGTGGACGGCGGTCAGGT
CACCGAGGCCTACGCGTCCTCGACGATGTGGCGCGGCATCGAGACGATCCTGACCGGCCGGGACCCCCGGGACGCCTGGC
TGTTCGCTCAGCGGATCTGCGGTGTGTGCACCTCGGTGCACGCGCTGGCCTCGGTGCGGGCGGTGGAGGACGCGGTCGGC
GCCGTCCCGCCGCTGAACGCCCGGCTGCTGCGGGACCTGATCGCCACGTCACTGTGTGTGCACGACCATGTCGTCCACTT
CTACCACCTGCAGGCCCTGGACTGGGTCGATCCGACCGCGGCGCTCGCGGCCGACCCGGTGCGTGCCGCGGCGCTGGCGC
AGTCCATCTCCGACTATCCGCGGTCCACGGCCGGTCTGTTCGCCTCGGTGCAGACCCGGCTCAAGGTCTTCCTGGAAAGC
GGGAAGATCGGGCCGTTCACCAACGGCTACTGGGGGCATCCGGCCTACCGGCTGAGCCCGGAGCTGAACCTGGTGGCGTT
CAGCCACTATCTCGACGCGCTGGACTTCCAGCGGGACTACATCCGGGTGCACGCCCTGCTCGGCGGAAAGAACCCGCACC
CGCAGACCTACGTGGTCGGCGGAATGGCGTCCCCGATCGACCTCAACAGCCAGGACGCCATCAACGCGAACACATTGCAG
GAGGTGACGCAGATCCTGCAGCGGGGCCTCGAATTCGTCGAGCAGGTCTTCCTGCCGGACCTGCAGGCGATCGGCGCCGC
CTATCCCGAGTGGACGACCTACGGTCGGGGCCTGGGCTCCTACATGGTGTTCGGTGACTATTCACTGTCGCCGCCGGGCA
TCGCCCGGCCACCCCGGGACGGCCTTTTCCCCGGCGGGATCATCCGGAACGGGAACCTCGCGGTGAAACCGGAACCCTTC
GACCCGTCCGGTATCGCCGAGTCCGTCGCCCATTCGTGGTTCCGCTACGACGATCCCGGCGCCGCGCTGCCCCCCTGGAA
GGGTGAGACGACCCCGCAGTACACCGGACCTGAACCCCCTTTCGAGCAGCTCGACGTCGCCGGGAAGTACACCTGGCTGA
AGGCGCCGCGCTACCAGGGAGCGGCCATGGAGGTCGGCCCGGTGGCCCGCATGCTCGTCGGCTACACCTCCGGGGACGCC
CGTATCCGCCCGCTCGTGCAGGGCGCGCTCGACGCCCTGCGACTGCCGCCCGAGGCACTGATGTCCACCCTCGGCCGGGT
GGTGGCCCGCGGCCTGGAGACCAGGCTGATGGCGCAGTACTCCCTGGAGCTCGTCAACCGGCTGCGTGACAACGTCGCCG
CCGGCGACCTGGCCGTCGCCGACACCGGGCGATGGCGTCCGACATCCTGGCCGGAGGGCGCCCTGCTCGGCGTCGGCTTC
CACGAGGCGCCCCGCGGGTCGCTGTCGCACTGGGTCGTCATCGAGGACGGCCGGATCCGCAACTACCAGGCCGTGGTGCC
CACCACCTGGAACGCCAGCCCCCGCGACGCGGAGGGGAATCCCGGCGCCTACGAGGCCGCGCTAGTCGGCACACCCGTCG
CCGATCCCCAGCGGCCGCTGGAGATCCTGCGCACCCTGCACTCCTTCGACCCCTGCATGGCCTGCGCGGCGCACATCTAC
GACGTCGAGGGCCGCGACATCATCGAGGTGCGGGTCCAGTGA

Upstream 100 bases:

>100_bases
ACGACGAAGCGCCCACAGACGAAGCGGCCACAGACGAAGCGGCCACAGACAAGGCACACGCCGACAAGGCGCACGGCGAC
GGAGAGACGGGCGAAGCGAG

Downstream 100 bases:

>100_bases
GCGTGACCGCGCCCGCCCCGCACACCGAGCACGCGGAGCTCGTCCGGTTCCGGGTCTGGGATCTTCCGGTCCGCCTCATC
CACTGGCTGCTGGTGCTGGA

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MSRLVIDPVSRIEGHLRVEVQVDGGQVTEAYASSTMWRGIETILTGRDPRDAWLFAQRICGVCTSVHALASVRAVEDAVG
AVPPLNARLLRDLIATSLCVHDHVVHFYHLQALDWVDPTAALAADPVRAAALAQSISDYPRSTAGLFASVQTRLKVFLES
GKIGPFTNGYWGHPAYRLSPELNLVAFSHYLDALDFQRDYIRVHALLGGKNPHPQTYVVGGMASPIDLNSQDAINANTLQ
EVTQILQRGLEFVEQVFLPDLQAIGAAYPEWTTYGRGLGSYMVFGDYSLSPPGIARPPRDGLFPGGIIRNGNLAVKPEPF
DPSGIAESVAHSWFRYDDPGAALPPWKGETTPQYTGPEPPFEQLDVAGKYTWLKAPRYQGAAMEVGPVARMLVGYTSGDA
RIRPLVQGALDALRLPPEALMSTLGRVVARGLETRLMAQYSLELVNRLRDNVAAGDLAVADTGRWRPTSWPEGALLGVGF
HEAPRGSLSHWVVIEDGRIRNYQAVVPTTWNASPRDAEGNPGAYEAALVGTPVADPQRPLEILRTLHSFDPCMACAAHIY
DVEGRDIIEVRVQ

Sequences:

>Translated_573_residues
MSRLVIDPVSRIEGHLRVEVQVDGGQVTEAYASSTMWRGIETILTGRDPRDAWLFAQRICGVCTSVHALASVRAVEDAVG
AVPPLNARLLRDLIATSLCVHDHVVHFYHLQALDWVDPTAALAADPVRAAALAQSISDYPRSTAGLFASVQTRLKVFLES
GKIGPFTNGYWGHPAYRLSPELNLVAFSHYLDALDFQRDYIRVHALLGGKNPHPQTYVVGGMASPIDLNSQDAINANTLQ
EVTQILQRGLEFVEQVFLPDLQAIGAAYPEWTTYGRGLGSYMVFGDYSLSPPGIARPPRDGLFPGGIIRNGNLAVKPEPF
DPSGIAESVAHSWFRYDDPGAALPPWKGETTPQYTGPEPPFEQLDVAGKYTWLKAPRYQGAAMEVGPVARMLVGYTSGDA
RIRPLVQGALDALRLPPEALMSTLGRVVARGLETRLMAQYSLELVNRLRDNVAAGDLAVADTGRWRPTSWPEGALLGVGF
HEAPRGSLSHWVVIEDGRIRNYQAVVPTTWNASPRDAEGNPGAYEAALVGTPVADPQRPLEILRTLHSFDPCMACAAHIY
DVEGRDIIEVRVQ
>Mature_572_residues
SRLVIDPVSRIEGHLRVEVQVDGGQVTEAYASSTMWRGIETILTGRDPRDAWLFAQRICGVCTSVHALASVRAVEDAVGA
VPPLNARLLRDLIATSLCVHDHVVHFYHLQALDWVDPTAALAADPVRAAALAQSISDYPRSTAGLFASVQTRLKVFLESG
KIGPFTNGYWGHPAYRLSPELNLVAFSHYLDALDFQRDYIRVHALLGGKNPHPQTYVVGGMASPIDLNSQDAINANTLQE
VTQILQRGLEFVEQVFLPDLQAIGAAYPEWTTYGRGLGSYMVFGDYSLSPPGIARPPRDGLFPGGIIRNGNLAVKPEPFD
PSGIAESVAHSWFRYDDPGAALPPWKGETTPQYTGPEPPFEQLDVAGKYTWLKAPRYQGAAMEVGPVARMLVGYTSGDAR
IRPLVQGALDALRLPPEALMSTLGRVVARGLETRLMAQYSLELVNRLRDNVAAGDLAVADTGRWRPTSWPEGALLGVGFH
EAPRGSLSHWVVIEDGRIRNYQAVVPTTWNASPRDAEGNPGAYEAALVGTPVADPQRPLEILRTLHSFDPCMACAAHIYD
VEGRDIIEVRVQ

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=584, Percent_Identity=47.6027397260274, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1789368, Length=593, Percent_Identity=45.531197301855, Blast_Score=517, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 62500; Mature: 62368

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVIDPVSRIEGHLRVEVQVDGGQVTEAYASSTMWRGIETILTGRDPRDAWLFAQRIC
CCCEEECCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GVCTSVHALASVRAVEDAVGAVPPLNARLLRDLIATSLCVHDHVVHFYHLQALDWVDPTA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ALAADPVRAAALAQSISDYPRSTAGLFASVQTRLKVFLESGKIGPFTNGYWGHPAYRLSP
HHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECC
ELNLVAFSHYLDALDFQRDYIRVHALLGGKNPHPQTYVVGGMASPIDLNSQDAINANTLQ
CCEEEEHHHHHHHHHHHHHHEEEEHEECCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHH
EVTQILQRGLEFVEQVFLPDLQAIGAAYPEWTTYGRGLGSYMVFGDYSLSPPGIARPPRD
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCC
GLFPGGIIRNGNLAVKPEPFDPSGIAESVAHSWFRYDDPGAALPPWKGETTPQYTGPEPP
CCCCCCEEECCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
FEQLDVAGKYTWLKAPRYQGAAMEVGPVARMLVGYTSGDARIRPLVQGALDALRLPPEAL
HHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHH
MSTLGRVVARGLETRLMAQYSLELVNRLRDNVAAGDLAVADTGRWRPTSWPEGALLGVGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEECCC
HEAPRGSLSHWVVIEDGRIRNYQAVVPTTWNASPRDAEGNPGAYEAALVGTPVADPQRPL
CCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHH
EILRTLHSFDPCMACAAHIYDVEGRDIIEVRVQ
HHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure 
SRLVIDPVSRIEGHLRVEVQVDGGQVTEAYASSTMWRGIETILTGRDPRDAWLFAQRIC
CCEEECCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GVCTSVHALASVRAVEDAVGAVPPLNARLLRDLIATSLCVHDHVVHFYHLQALDWVDPTA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ALAADPVRAAALAQSISDYPRSTAGLFASVQTRLKVFLESGKIGPFTNGYWGHPAYRLSP
HHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECC
ELNLVAFSHYLDALDFQRDYIRVHALLGGKNPHPQTYVVGGMASPIDLNSQDAINANTLQ
CCEEEEHHHHHHHHHHHHHHEEEEHEECCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHH
EVTQILQRGLEFVEQVFLPDLQAIGAAYPEWTTYGRGLGSYMVFGDYSLSPPGIARPPRD
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCC
GLFPGGIIRNGNLAVKPEPFDPSGIAESVAHSWFRYDDPGAALPPWKGETTPQYTGPEPP
CCCCCCEEECCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
FEQLDVAGKYTWLKAPRYQGAAMEVGPVARMLVGYTSGDARIRPLVQGALDALRLPPEAL
HHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHH
MSTLGRVVARGLETRLMAQYSLELVNRLRDNVAAGDLAVADTGRWRPTSWPEGALLGVGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEECCC
HEAPRGSLSHWVVIEDGRIRNYQAVVPTTWNASPRDAEGNPGAYEAALVGTPVADPQRPL
CCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHH
EILRTLHSFDPCMACAAHIYDVEGRDIIEVRVQ
HHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]