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The map label for this gene is hydA [H]

Identifier: 158315203

GI number: 158315203

Start: 4034026

End: 4035228

Strand: Direct

Name: hydA [H]

Synonym: Franean1_3403

Alternate gene names: 158315203

Gene position: 4034026-4035228 (Clockwise)

Preceding gene: 158315197

Following gene: 158315204

Centisome position: 44.91

GC content: 70.91

Gene sequence:

>1203_bases
ATGACGAGCGAATCCGGCCGGCCTTTGTCCGCGCGGCTGGCGGACGCGGGGGTCGACCGGCGGTCGTTCCTGAAATTCTG
CGCGGGTATCACCGCGACGCTCGCCCTGCCGGCCCAGTTGGCGCCCCGGGTCGCCTATGCGCTGGACAAGGTTGACCGTC
CCACCGTGATCTGGCTGGAATTCCAGGACTGCGCCGGTGACAGCGAGGCTTTCCTGCGTTCGCGCAACCCGTCGGCGGCG
GACCTGATTCTGGGGATGATCTCGCTCGACTACCACGAGACGGTGATGGCGGCCTCCGGCACAGCCGCGGAGAAGGCCCG
CGACGACGCGGTGGCGAAAGGCGGGCATCTCGTCGTCGTCGAGGGCTCCATTCCGACCGGAATTCCCGGGGCGTGCACGA
TCGGCGGCAAGTCGGCCGAGGACATCCTGCGTGAGGCGGTCCGTGGCGCGGCCGGCATCATCAACGTCGGCACCTGCTCG
GCCTTCGGTGGGATCCCGGCGGCCGGGCCGAACCCGACCGGGGCGGTCCGGGTCGAGGACGTCGTCGGCGGGGTTCCGGT
TGTGAACCTGACCGGTTGCCCGGTCAACGCGGACAACCTGACCGCCACCATCGTCCACCACCTGACCTTCGGTGAGTTCC
CGGCGCGGGACAGGTTCAGCCGGCCGCTGTTCGCCTACGGGCAGCGCATCCACGACACCTGCCAGCGGCGCGGCCACTTC
GACGCCGGCGAGTTCGCCGAGCAGTGGGGCGACGCGGGCCACCGCAACGGCTGGTGCCTGTACCGGCTGGGCTGCAAGGG
GCCGAGCACCTTCCACAACTGTCCCAGCGTCCGGTTCAACGGGGGGACGTCCTGGCCGGTGGCGGCCGGGCACGGCTGTG
TGGGCTGCTCCGAGCCGGGCTTCTGGGACACCATGTCGCCCTTCTACGACCGGCTGCCGCACGTGGCGACCTCCGGTTTC
GACCTCACCGCCGACAGGATCGGCGTCGGCGTGCTGGCGGCCACGGCGACCGGCTTCGCGGCGCACGGCGTCGGCAAGGT
CGTCCAGCACCGGGTCGCCGCCCGCCGCGAGAAGCAGGCCGCGCGGGACCTGGTGGAAGACGACGAAGCGCCCACAGACG
AAGCGGCCACAGACGAAGCGGCCACAGACAAGGCACACGCCGACAAGGCGCACGGCGACGGAGAGACGGGCGAAGCGAGA
TGA

Upstream 100 bases:

>100_bases
CAAGGGACGTCCGGCCCGTCCGTTCGGCTCGTATGGACTCCCGCGGCCGAGGCGATGAGGCTGCCGTTCGGCACCATGGC
GCGCACTAACGTCCACTCGC

Downstream 100 bases:

>100_bases
GTCGGCTTGTCATCGACCCGGTGTCGCGGATCGAGGGCCACCTGCGGGTCGAGGTGCAGGTGGACGGCGGTCAGGTCACC
GAGGCCTACGCGTCCTCGAC

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTSESGRPLSARLADAGVDRRSFLKFCAGITATLALPAQLAPRVAYALDKVDRPTVIWLEFQDCAGDSEAFLRSRNPSAA
DLILGMISLDYHETVMAASGTAAEKARDDAVAKGGHLVVVEGSIPTGIPGACTIGGKSAEDILREAVRGAAGIINVGTCS
AFGGIPAAGPNPTGAVRVEDVVGGVPVVNLTGCPVNADNLTATIVHHLTFGEFPARDRFSRPLFAYGQRIHDTCQRRGHF
DAGEFAEQWGDAGHRNGWCLYRLGCKGPSTFHNCPSVRFNGGTSWPVAAGHGCVGCSEPGFWDTMSPFYDRLPHVATSGF
DLTADRIGVGVLAATATGFAAHGVGKVVQHRVAARREKQAARDLVEDDEAPTDEAATDEAATDKAHADKAHGDGETGEAR

Sequences:

>Translated_400_residues
MTSESGRPLSARLADAGVDRRSFLKFCAGITATLALPAQLAPRVAYALDKVDRPTVIWLEFQDCAGDSEAFLRSRNPSAA
DLILGMISLDYHETVMAASGTAAEKARDDAVAKGGHLVVVEGSIPTGIPGACTIGGKSAEDILREAVRGAAGIINVGTCS
AFGGIPAAGPNPTGAVRVEDVVGGVPVVNLTGCPVNADNLTATIVHHLTFGEFPARDRFSRPLFAYGQRIHDTCQRRGHF
DAGEFAEQWGDAGHRNGWCLYRLGCKGPSTFHNCPSVRFNGGTSWPVAAGHGCVGCSEPGFWDTMSPFYDRLPHVATSGF
DLTADRIGVGVLAATATGFAAHGVGKVVQHRVAARREKQAARDLVEDDEAPTDEAATDEAATDKAHADKAHGDGETGEAR
>Mature_399_residues
TSESGRPLSARLADAGVDRRSFLKFCAGITATLALPAQLAPRVAYALDKVDRPTVIWLEFQDCAGDSEAFLRSRNPSAAD
LILGMISLDYHETVMAASGTAAEKARDDAVAKGGHLVVVEGSIPTGIPGACTIGGKSAEDILREAVRGAAGIINVGTCSA
FGGIPAAGPNPTGAVRVEDVVGGVPVVNLTGCPVNADNLTATIVHHLTFGEFPARDRFSRPLFAYGQRIHDTCQRRGHFD
AGEFAEQWGDAGHRNGWCLYRLGCKGPSTFHNCPSVRFNGGTSWPVAAGHGCVGCSEPGFWDTMSPFYDRLPHVATSGFD
LTADRIGVGVLAATATGFAAHGVGKVVQHRVAARREKQAARDLVEDDEAPTDEAATDEAATDKAHADKAHGDGETGEAR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=319, Percent_Identity=45.141065830721, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1789371, Length=289, Percent_Identity=47.4048442906574, Blast_Score=271, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 41916; Mature: 41785

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSESGRPLSARLADAGVDRRSFLKFCAGITATLALPAQLAPRVAYALDKVDRPTVIWLE
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
FQDCAGDSEAFLRSRNPSAADLILGMISLDYHETVMAASGTAAEKARDDAVAKGGHLVVV
ECCCCCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEE
EGSIPTGIPGACTIGGKSAEDILREAVRGAAGIINVGTCSAFGGIPAAGPNPTGAVRVED
ECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEECCCHHHCCCCCCCCCCCCCCEEEHH
VVGGVPVVNLTGCPVNADNLTATIVHHLTFGEFPARDRFSRPLFAYGQRIHDTCQRRGHF
HHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCC
DAGEFAEQWGDAGHRNGWCLYRLGCKGPSTFHNCPSVRFNGGTSWPVAAGHGCVGCSEPG
CHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
FWDTMSPFYDRLPHVATSGFDLTADRIGVGVLAATATGFAAHGVGKVVQHRVAARREKQA
CCCCCCHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDLVEDDEAPTDEAATDEAATDKAHADKAHGDGETGEAR
HHHHHHCCCCCCCCCCCCHHHCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSESGRPLSARLADAGVDRRSFLKFCAGITATLALPAQLAPRVAYALDKVDRPTVIWLE
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
FQDCAGDSEAFLRSRNPSAADLILGMISLDYHETVMAASGTAAEKARDDAVAKGGHLVVV
ECCCCCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEE
EGSIPTGIPGACTIGGKSAEDILREAVRGAAGIINVGTCSAFGGIPAAGPNPTGAVRVED
ECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCEEECCCHHHCCCCCCCCCCCCCCEEEHH
VVGGVPVVNLTGCPVNADNLTATIVHHLTFGEFPARDRFSRPLFAYGQRIHDTCQRRGHF
HHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCC
DAGEFAEQWGDAGHRNGWCLYRLGCKGPSTFHNCPSVRFNGGTSWPVAAGHGCVGCSEPG
CHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
FWDTMSPFYDRLPHVATSGFDLTADRIGVGVLAATATGFAAHGVGKVVQHRVAARREKQA
CCCCCCHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDLVEDDEAPTDEAATDEAATDKAHADKAHGDGETGEAR
HHHHHHCCCCCCCCCCCCHHHCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]