BacMap

Definition	Frankia sp. EAN1pec chromosome, complete genome.
Accession	NC_009921
Length	8,982,042

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The map label for this gene is hdhA [C]

Identifier: 158315184

GI number: 158315184

Start: 4009390

End: 4010163

Strand: Direct

Name: hdhA [C]

Synonym: Franean1_3384

Alternate gene names: 158315184

Gene position: 4009390-4010163 (Clockwise)

Preceding gene: 158315183

Following gene: 158315185

Centisome position: 44.64

GC content: 66.54

Gene sequence:

>774_bases
ATGCGCGGACTGGCGGACAAGGTCTTCATCGTGGCGGGCGGGGCCACCGGAATCGGTGCCGGGGCGGCAAAAAGGCTCGC
CGAGGAGAGTGCCAAGGTGGTAGTTGGCGACATCAACCTCGCCGGAGCGCAGAGCACCGTCAGAAAGATAAACGAGGCCG
GCGGCAGCGCGGTCGCTGTCCACTTCGACCTCGCCGACGAGCACTCGGTCAATGAGATGGTCGATACGACGCTGGAGAAG
TTCGGCGCCGTCCACGGCCTGTTCAACGTCGGCGCGGACCTGTCGCCCGGCAACCTCGGGCGGGACAAGTCGGTACTGGA
CACCGATCTCGACGTGTGGCATCGAACACTGGACGTCAACGTGGTCGGGTTCGTGCGGACCATTCGCGCCGTCCTGCCTC
ATCTGCTCGCGAACGGTGGCGGTGGCATCGTCAACACCTCGTCCGGTGCGGCGGTCAGTGCAGGCGACCGTCTCCGGCCC
GCCTATGCGGCCTCGAAATCGGCGGTCAATGCGCTCACGCGGCACGTCGCCGGGAACTGGGGATCCAGGGGCGTGCGCTG
CAACGGGGTGATGCCGGGTCTGGTGATGGGCGAGACACAGGAGATCCAGAACGACGTCGCGCTCCAGGAGAGGTTCCTGA
AAGAGGTCCCCACCACCCGGCTGGGCCGTCCGAGCGATCTGGCCGCCGTCGTGTCCTTCCTGCTCTCGGACGACGCCGAA
TGGATCAACGGTCAGGTCTGGGCGATCGACGGCGGCGCCAACATGCGTGCCTGA

Upstream 100 bases:

>100_bases
ATGGGATGTGCTGTCGTCGCGCCAGGGGCGCGGCCGATGATCCTGGCGCGACGACACGCGGGGGAATGGTTTTTCAGTAC
CAGAGTGCGGGAGCAGTGAG

Downstream 100 bases:

>100_bases
CGAACGACCCGGCATCCGGCCGGGGACGGCCGACACCGTGAACGCGGTGACCGGCATCGGCAGGCCGGCTGGGAGGCGCA
GGTCGTGCTGCGCGACATCG

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MRGLADKVFIVAGGATGIGAGAAKRLAEESAKVVVGDINLAGAQSTVRKINEAGGSAVAVHFDLADEHSVNEMVDTTLEK
FGAVHGLFNVGADLSPGNLGRDKSVLDTDLDVWHRTLDVNVVGFVRTIRAVLPHLLANGGGGIVNTSSGAAVSAGDRLRP
AYAASKSAVNALTRHVAGNWGSRGVRCNGVMPGLVMGETQEIQNDVALQERFLKEVPTTRLGRPSDLAAVVSFLLSDDAE
WINGQVWAIDGGANMRA

Sequences:

>Translated_257_residues
MRGLADKVFIVAGGATGIGAGAAKRLAEESAKVVVGDINLAGAQSTVRKINEAGGSAVAVHFDLADEHSVNEMVDTTLEK
FGAVHGLFNVGADLSPGNLGRDKSVLDTDLDVWHRTLDVNVVGFVRTIRAVLPHLLANGGGGIVNTSSGAAVSAGDRLRP
AYAASKSAVNALTRHVAGNWGSRGVRCNGVMPGLVMGETQEIQNDVALQERFLKEVPTTRLGRPSDLAAVVSFLLSDDAE
WINGQVWAIDGGANMRA
>Mature_257_residues
MRGLADKVFIVAGGATGIGAGAAKRLAEESAKVVVGDINLAGAQSTVRKINEAGGSAVAVHFDLADEHSVNEMVDTTLEK
FGAVHGLFNVGADLSPGNLGRDKSVLDTDLDVWHRTLDVNVVGFVRTIRAVLPHLLANGGGGIVNTSSGAAVSAGDRLRP
AYAASKSAVNALTRHVAGNWGSRGVRCNGVMPGLVMGETQEIQNDVALQERFLKEVPTTRLGRPSDLAAVVSFLLSDDAE
WINGQVWAIDGGANMRA

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=263, Percent_Identity=30.4182509505703, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI32483357, Length=254, Percent_Identity=31.8897637795276, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI15277342, Length=255, Percent_Identity=30.5882352941176, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI66933014, Length=238, Percent_Identity=30.2521008403361, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40254992, Length=251, Percent_Identity=28.6852589641434, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=29.3172690763052, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI126723750, Length=257, Percent_Identity=28.4046692607004, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI10190704, Length=254, Percent_Identity=28.3464566929134, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI4758504, Length=267, Percent_Identity=28.8389513108614, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI83715985, Length=262, Percent_Identity=28.2442748091603, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4503301, Length=231, Percent_Identity=25.5411255411255, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=25.974025974026, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787905, Length=246, Percent_Identity=29.6747967479675, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=29.4820717131474, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=27.6679841897233, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=29.3893129770992, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367175, Length=249, Percent_Identity=30.5220883534137, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789378, Length=261, Percent_Identity=27.9693486590038, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=26.4, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082160, Length=254, Percent_Identity=24.8031496062992, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=29.1828793774319, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367365, Length=259, Percent_Identity=27.4131274131274, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=260, Percent_Identity=30, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=32.0158102766798, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17560676, Length=261, Percent_Identity=28.735632183908, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994600, Length=261, Percent_Identity=31.8007662835249, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17560332, Length=270, Percent_Identity=31.8518518518519, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538480, Length=263, Percent_Identity=30.4182509505703, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562910, Length=270, Percent_Identity=30, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI72000259, Length=270, Percent_Identity=29.2592592592593, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17562906, Length=275, Percent_Identity=31.2727272727273, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17563726, Length=271, Percent_Identity=29.8892988929889, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=30.4832713754647, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=29.9625468164794, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562904, Length=273, Percent_Identity=28.9377289377289, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17551412, Length=268, Percent_Identity=28.3582089552239, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17565030, Length=265, Percent_Identity=30.9433962264151, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17560150, Length=268, Percent_Identity=29.4776119402985, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562908, Length=265, Percent_Identity=31.3207547169811, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560220, Length=270, Percent_Identity=28.1481481481481, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17562990, Length=271, Percent_Identity=28.7822878228782, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=273, Percent_Identity=29.6703296703297, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=266, Percent_Identity=27.8195488721804, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI193204405, Length=273, Percent_Identity=29.3040293040293, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17536651, Length=253, Percent_Identity=26.0869565217391, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=253, Percent_Identity=26.4822134387352, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=251, Percent_Identity=28.2868525896414, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17538182, Length=264, Percent_Identity=26.8939393939394, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=254, Percent_Identity=26.3779527559055, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536025, Length=269, Percent_Identity=27.5092936802974, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508651, Length=260, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=160, Percent_Identity=31.25, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322227, Length=253, Percent_Identity=28.8537549407115, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322861, Length=196, Percent_Identity=28.0612244897959, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355319, Length=263, Percent_Identity=32.319391634981, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21357041, Length=261, Percent_Identity=34.8659003831418, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI23397609, Length=261, Percent_Identity=32.9501915708812, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28571526, Length=261, Percent_Identity=32.9501915708812, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=30.4347826086957, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17737361, Length=264, Percent_Identity=31.4393939393939, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24644339, Length=258, Percent_Identity=29.0697674418605, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI18859837, Length=243, Percent_Identity=27.9835390946502, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24643142, Length=256, Percent_Identity=27.34375, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644337, Length=221, Percent_Identity=28.0542986425339, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 26716; Mature: 26716

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLADKVFIVAGGATGIGAGAAKRLAEESAKVVVGDINLAGAQSTVRKINEAGGSAVAV
CCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEE
HFDLADEHSVNEMVDTTLEKFGAVHGLFNVGADLSPGNLGRDKSVLDTDLDVWHRTLDVN
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
VVGFVRTIRAVLPHLLANGGGGIVNTSSGAAVSAGDRLRPAYAASKSAVNALTRHVAGNW
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
GSRGVRCNGVMPGLVMGETQEIQNDVALQERFLKEVPTTRLGRPSDLAAVVSFLLSDDAE
CCCCEEECCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCH
WINGQVWAIDGGANMRA
HHCCEEEEEECCCCCCC
>Mature Secondary Structure
MRGLADKVFIVAGGATGIGAGAAKRLAEESAKVVVGDINLAGAQSTVRKINEAGGSAVAV
CCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEE
HFDLADEHSVNEMVDTTLEKFGAVHGLFNVGADLSPGNLGRDKSVLDTDLDVWHRTLDVN
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
VVGFVRTIRAVLPHLLANGGGGIVNTSSGAAVSAGDRLRPAYAASKSAVNALTRHVAGNW
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
GSRGVRCNGVMPGLVMGETQEIQNDVALQERFLKEVPTTRLGRPSDLAAVVSFLLSDDAE
CCCCEEECCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCH
WINGQVWAIDGGANMRA
HHCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9655913; 12597275 [H]