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The map label for this gene is gsiB [H]

Identifier: 158315092

GI number: 158315092

Start: 3898027

End: 3899586

Strand: Direct

Name: gsiB [H]

Synonym: Franean1_3291

Alternate gene names: 158315092

Gene position: 3898027-3899586 (Clockwise)

Preceding gene: 158315090

Following gene: 158315093

Centisome position: 43.4

GC content: 65.45

Gene sequence:

>1560_bases
ATGTTGCGTAGGAGACGTTTCCTGTTTGTGGCAGTAGCCAGTGGTCTTGCGGCGGTCCTCACCGCCTGCGGTGGCTCCGG
TTCCCCCTCGACGTCATCTGCCTCAGGTGAACCGGTCGCCGGCGGTCACGGCCGGATCCTCATGTTGGGCGAGCCGCGCA
GTCTGGATCCAGCGGCGCTCGGCAACGCTTATGCGATCAATGCCGCCGTGGGTAACGCCTTATACGGAACATTGATGACC
GACGACGACAGCGGTAAGATTCAGTTCTCCATGGCGGAGTCGTTCACCACCGCCGACAACGGCGCCACTTTCGAACTGAA
ACTGCGGCCGGATCTGGTGTTCTCCGACGGGGCGCCGCTGAATGCCGCTGCAGTGAAGTTCAACTGGGACCGCCTGAAGA
ATCCTGCTAACGGCGCCACCTCCCTCGCCCAGGCATCCGTGGCCGCCTCTACCGAGGTGGTGGACGACCTCACGCTCAAG
GTCACGATGGTCACTCCCATGCCCAGGTACGCGGGCTCCGTCATCACCTCGTCGATGAACTGGATCGCCTCGCCCGCCGT
GCTGGAGAAGGGAACGGAGGCCTTCGACAAGGCCCCGATCGGTGCGGGTCCCTTCACTCTGAAGAGCTGGACCCGCCAGG
CCAGCATCGAGCTGGCGAAGAACCCCAGGTACTGGGACGCCCCCAAGCCCTATCTGGACACGCTCACCCTGCGCACGCTT
GCCGACACCAACCAGCGCTTCAACACGGTCCTTTCCGGCACCGCGGACGCGGCCGCCGAGTCCAGCTGGCAGAACTTCTC
GAAGGCCGAGGAACAGGGCCTGGCCCTCGGCAGGCAGAACGTCAACGGTGGACTGTTCCTCACGATGAACTCACGCCGGG
CGCCGTTCGACGATCCCCGCGCCCGCCGGGCCATCGCCGCCGCGCTGGACCTCGACGCTCTCAACCTGGCCGTCTACAAC
GGCCTGGGAAAGCCGGTCGAGACGCTGTTCACCGAGGGCTCGCCCTTCTACTCGAACATTCCGCTCCGCAAGGTCGACAA
GGCTGCCGCGCAGCGCCTGTTCGACGAGCTGGCCGCGGCGGGCAAACCCGTCTCCTTCACGTTCTCCGCGTTCCCCAGCA
CAGAGAACCGGGCGATGGCGGAGAACGTCCAGGCACAGCTCAGCACCTTTGACAACGTCAAGGTCAACATCGAGCCCCTC
GACCAGTCCAGGCTCGGAGAGCTGTATTCGAAGCGGGACTTCGACATGGTCACCCTGTCCTCCTTCTTCTACGACCCCGA
CCCGGTGCTGTCGACGGTCTTCGACGGGAGCTCGCCGTCCAACCTGTCCGGCATCAACGACCCGGAACTCAATGAGGCCC
TGCAGGCCGGCCGCACCGCGACGAGCGACGAGGAGCGCGGGAAGGCCTACGAGACCGTGCAGCGGCGGCTCGCGGACCAG
GTCCCGGTGGTCTTCATCACGCGGGTGGCGCTGGGTGCCATCGGCGGGCAGAACGTCGGCGGCATCAGGCTCTACGGCAA
CGGCTCGCTGCTGCCCGAGGAGCTGTGGATCAGCAAGTAG

Upstream 100 bases:

>100_bases
GATCGCCGATGGACGCCGGCGGAGGCCGCCGCCGGGCGGCCTGGAAATGACTGTTCACAAGATCGAGTTCACGCATGATT
CTGGTCCTCGAAAGGGTGGC

Downstream 100 bases:

>100_bases
CGGCGCTCGGCAGCACTGGACAGGGAGGATCCGGTGGTCGAGCCGCCTCTCGCGGGCGCCGAGGGACGGCTCGACCACGG
TCGCGTCCAGCTACGGCCAG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MLRRRRFLFVAVASGLAAVLTACGGSGSPSTSSASGEPVAGGHGRILMLGEPRSLDPAALGNAYAINAAVGNALYGTLMT
DDDSGKIQFSMAESFTTADNGATFELKLRPDLVFSDGAPLNAAAVKFNWDRLKNPANGATSLAQASVAASTEVVDDLTLK
VTMVTPMPRYAGSVITSSMNWIASPAVLEKGTEAFDKAPIGAGPFTLKSWTRQASIELAKNPRYWDAPKPYLDTLTLRTL
ADTNQRFNTVLSGTADAAAESSWQNFSKAEEQGLALGRQNVNGGLFLTMNSRRAPFDDPRARRAIAAALDLDALNLAVYN
GLGKPVETLFTEGSPFYSNIPLRKVDKAAAQRLFDELAAAGKPVSFTFSAFPSTENRAMAENVQAQLSTFDNVKVNIEPL
DQSRLGELYSKRDFDMVTLSSFFYDPDPVLSTVFDGSSPSNLSGINDPELNEALQAGRTATSDEERGKAYETVQRRLADQ
VPVVFITRVALGAIGGQNVGGIRLYGNGSLLPEELWISK

Sequences:

>Translated_519_residues
MLRRRRFLFVAVASGLAAVLTACGGSGSPSTSSASGEPVAGGHGRILMLGEPRSLDPAALGNAYAINAAVGNALYGTLMT
DDDSGKIQFSMAESFTTADNGATFELKLRPDLVFSDGAPLNAAAVKFNWDRLKNPANGATSLAQASVAASTEVVDDLTLK
VTMVTPMPRYAGSVITSSMNWIASPAVLEKGTEAFDKAPIGAGPFTLKSWTRQASIELAKNPRYWDAPKPYLDTLTLRTL
ADTNQRFNTVLSGTADAAAESSWQNFSKAEEQGLALGRQNVNGGLFLTMNSRRAPFDDPRARRAIAAALDLDALNLAVYN
GLGKPVETLFTEGSPFYSNIPLRKVDKAAAQRLFDELAAAGKPVSFTFSAFPSTENRAMAENVQAQLSTFDNVKVNIEPL
DQSRLGELYSKRDFDMVTLSSFFYDPDPVLSTVFDGSSPSNLSGINDPELNEALQAGRTATSDEERGKAYETVQRRLADQ
VPVVFITRVALGAIGGQNVGGIRLYGNGSLLPEELWISK
>Mature_519_residues
MLRRRRFLFVAVASGLAAVLTACGGSGSPSTSSASGEPVAGGHGRILMLGEPRSLDPAALGNAYAINAAVGNALYGTLMT
DDDSGKIQFSMAESFTTADNGATFELKLRPDLVFSDGAPLNAAAVKFNWDRLKNPANGATSLAQASVAASTEVVDDLTLK
VTMVTPMPRYAGSVITSSMNWIASPAVLEKGTEAFDKAPIGAGPFTLKSWTRQASIELAKNPRYWDAPKPYLDTLTLRTL
ADTNQRFNTVLSGTADAAAESSWQNFSKAEEQGLALGRQNVNGGLFLTMNSRRAPFDDPRARRAIAAALDLDALNLAVYN
GLGKPVETLFTEGSPFYSNIPLRKVDKAAAQRLFDELAAAGKPVSFTFSAFPSTENRAMAENVQAQLSTFDNVKVNIEPL
DQSRLGELYSKRDFDMVTLSSFFYDPDPVLSTVFDGSSPSNLSGINDPELNEALQAGRTATSDEERGKAYETVQRRLADQ
VPVVFITRVALGAIGGQNVGGIRLYGNGSLLPEELWISK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=361, Percent_Identity=29.3628808864266, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787762, Length=472, Percent_Identity=24.5762711864407, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789966, Length=444, Percent_Identity=25.2252252252252, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787495, Length=490, Percent_Identity=23.0612244897959, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789397, Length=503, Percent_Identity=23.0616302186879, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787551, Length=497, Percent_Identity=22.3340040241449, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789887, Length=469, Percent_Identity=23.8805970149254, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081878, Length=483, Percent_Identity=23.3954451345756, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55434; Mature: 55434

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRRRFLFVAVASGLAAVLTACGGSGSPSTSSASGEPVAGGHGRILMLGEPRSLDPAAL
CCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHC
GNAYAINAAVGNALYGTLMTDDDSGKIQFSMAESFTTADNGATFELKLRPDLVFSDGAPL
CCCEEEEHHHCCCEEEEEEECCCCCEEEEEECCCEEECCCCCEEEEEECCCEEECCCCCC
NAAAVKFNWDRLKNPANGATSLAQASVAASTEVVDDLTLKVTMVTPMPRYAGSVITSSMN
CEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCHH
WIASPAVLEKGTEAFDKAPIGAGPFTLKSWTRQASIELAKNPRYWDAPKPYLDTLTLRTL
HHCCCHHHHCCCHHHHCCCCCCCCCHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHH
ADTNQRFNTVLSGTADAAAESSWQNFSKAEEQGLALGRQNVNGGLFLTMNSRRAPFDDPR
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHH
ARRAIAAALDLDALNLAVYNGLGKPVETLFTEGSPFYSNIPLRKVDKAAAQRLFDELAAA
HHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GKPVSFTFSAFPSTENRAMAENVQAQLSTFDNVKVNIEPLDQSRLGELYSKRDFDMVTLS
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCCCEEEHH
SFFYDPDPVLSTVFDGSSPSNLSGINDPELNEALQAGRTATSDEERGKAYETVQRRLADQ
HHCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
VPVVFITRVALGAIGGQNVGGIRLYGNGSLLPEELWISK
CCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCCCC
>Mature Secondary Structure
MLRRRRFLFVAVASGLAAVLTACGGSGSPSTSSASGEPVAGGHGRILMLGEPRSLDPAAL
CCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHC
GNAYAINAAVGNALYGTLMTDDDSGKIQFSMAESFTTADNGATFELKLRPDLVFSDGAPL
CCCEEEEHHHCCCEEEEEEECCCCCEEEEEECCCEEECCCCCEEEEEECCCEEECCCCCC
NAAAVKFNWDRLKNPANGATSLAQASVAASTEVVDDLTLKVTMVTPMPRYAGSVITSSMN
CEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCHH
WIASPAVLEKGTEAFDKAPIGAGPFTLKSWTRQASIELAKNPRYWDAPKPYLDTLTLRTL
HHCCCHHHHCCCHHHHCCCCCCCCCHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHH
ADTNQRFNTVLSGTADAAAESSWQNFSKAEEQGLALGRQNVNGGLFLTMNSRRAPFDDPR
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHH
ARRAIAAALDLDALNLAVYNGLGKPVETLFTEGSPFYSNIPLRKVDKAAAQRLFDELAAA
HHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GKPVSFTFSAFPSTENRAMAENVQAQLSTFDNVKVNIEPLDQSRLGELYSKRDFDMVTLS
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCCCEEEHH
SFFYDPDPVLSTVFDGSSPSNLSGINDPELNEALQAGRTATSDEERGKAYETVQRRLADQ
HHCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
VPVVFITRVALGAIGGQNVGGIRLYGNGSLLPEELWISK
CCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA