Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 158314797

GI number: 158314797

Start: 3506257

End: 3507840

Strand: Direct

Name: gsiB [H]

Synonym: Franean1_2981

Alternate gene names: 158314797

Gene position: 3506257-3507840 (Clockwise)

Preceding gene: 158314796

Following gene: 158314806

Centisome position: 39.04

GC content: 65.21

Gene sequence:

>1584_bases
ATGATTCGTGGAGCACGATTAGGCGCCGTGGCGGTCACCTGCTGCGCCGCGCTTGTCCTCGCCGCCTGTGGAGGCGGCGA
GTCAGACGGTTCCTCAAGGACCTCCGAGAACGGGGCCTCCGGCGCGACCGGCAACCCGGTGCCCGGCGGCGAAGGGCGGA
TCCTCCTGCTGAGCGAGCCGCGCAGCCTCGACCCGGCGGCGCTCAACAACGTGTACGCGGCCGGCGCTGCCGTCGGCAAC
GCCCTGTACGGCACGCTGATGACCAACAACGAGGCCGGCGAGATCCAATACTCGATGGCCGAGTCCTTCACCACGCCGGA
CAACGGCGCCACCTTCGAGCTGAAGCTCCGGCCGGGGCTGGCCTTCTCGGACGGCACCGCGCTGACCGCCGAGGCCGTCA
AATACAACTGGGACCGGATCAAGGACCCCAAGCTGGGCGCTTTCAGCCGGGCCGAGGCAGCGATGATCGCCTCGTCTGAG
GTGGAGGACGAGACCACTCTCAAGGTCACCATGGTCGCGCCGGTACCGAAGTACGCCCAGGCCATCGTCACCTCGACGCT
CAACTGGATCGCGGAGCCCGCCGCTCTGGAGAAAGGGCAGCAGAAATTTGACACCCAGCCGATCGGCGCCGGCCCCTTCA
CGCTGAGTAAATGGACACGCCAGGCCGAGATGGAGCTCGTCAGGAACACCCGCTACTGGGATGCTCCCAAGCCCTACCTC
GACCGTCTTTCTTTGCGGCCGGCGACCGACAGTAGTCAGCGCTACAACACCGTGCTCACGGGAGGTGCGGACCTCGCCGT
AGAATCCAGCTGGGTCAACCTGGACAAAGCGAAGCAGGCCCGACTCTCGACGAACGTGATGCAGCTCAGTGGCGGGATAT
TCATCGCCCTGAACCTGCGCAGCGAACCATTCTCCGATATTCGCGCCCGGCAGGCCCTCGCAGCCGCGATCGACATCGAG
ACACTGAACCTCGCCGCTTACAGCGGCACCGCCGAGGCGGCCGATACCCTTTTCAGTAAAATTTCTCCGTATCATTCTGA
TGTCCCGCTGCACACCACCGACCACGACAAGGCCCAGCGCCTGTTCGACGAACTGGCCGGTGAGGGAAAGCCGGTCACCT
TCACCTTCAAAACACCTCCGACGACCGAGAACAGGGCGGTCGCGGAAAACATCCAAACCCAGCTCAGCACCTTCAGGAAC
GTGAAAGCCGAAGTCAAGGTCATCGAGGTCGCGGAGTTCTCTCAGCTGCGCACAACTCATGACTTCGACGCGGCGGCCTC
CTCGGCGCTGTTCCAGGACCCTGACCCGCGGCTGACCACCACGTTCGCCGGCGACTCGCCGGCCAACCTGACCGGCATCG
ACGACGCGGTACTGAACGAGAGCCTGCAGGCCGGCCGGGTCGCGCGGACCGAGGCGGAAGCCAAGGACGCCTATGTGACC
GTGCAGGAACGCCTGGCGGTGGTCACTCCAGTGATCTTCACCGTGCGGGCGGCACCCAGCACCATCTCGAGCCCCAACGT
GGGCGGCATCGTCCAGTACGGCATCGGCTCGCTGCTCCCCAGCGAGCTGTGGATCAAGCCCTAG

Upstream 100 bases:

>100_bases
GGCGTCATTCGGGCCATCCCCGCCCGGATCCACTGTTTCCGGTACCTCTCTAGTCCGAACCCCGTCGAAAACTGGCGCGA
ACCCACTTGAAAGGTTCTCG

Downstream 100 bases:

>100_bases
TAGGCTGTCGCCGCTAATGTGTGGGGTATAGGTGGCGGAGTTTGGTGCGGGCGTCGTCGGTGGTGAAGTGCCAGTTGACT
TGAGGGGCTTTGCTGCTGAC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MIRGARLGAVAVTCCAALVLAACGGGESDGSSRTSENGASGATGNPVPGGEGRILLLSEPRSLDPAALNNVYAAGAAVGN
ALYGTLMTNNEAGEIQYSMAESFTTPDNGATFELKLRPGLAFSDGTALTAEAVKYNWDRIKDPKLGAFSRAEAAMIASSE
VEDETTLKVTMVAPVPKYAQAIVTSTLNWIAEPAALEKGQQKFDTQPIGAGPFTLSKWTRQAEMELVRNTRYWDAPKPYL
DRLSLRPATDSSQRYNTVLTGGADLAVESSWVNLDKAKQARLSTNVMQLSGGIFIALNLRSEPFSDIRARQALAAAIDIE
TLNLAAYSGTAEAADTLFSKISPYHSDVPLHTTDHDKAQRLFDELAGEGKPVTFTFKTPPTTENRAVAENIQTQLSTFRN
VKAEVKVIEVAEFSQLRTTHDFDAAASSALFQDPDPRLTTTFAGDSPANLTGIDDAVLNESLQAGRVARTEAEAKDAYVT
VQERLAVVTPVIFTVRAAPSTISSPNVGGIVQYGIGSLLPSELWIKP

Sequences:

>Translated_527_residues
MIRGARLGAVAVTCCAALVLAACGGGESDGSSRTSENGASGATGNPVPGGEGRILLLSEPRSLDPAALNNVYAAGAAVGN
ALYGTLMTNNEAGEIQYSMAESFTTPDNGATFELKLRPGLAFSDGTALTAEAVKYNWDRIKDPKLGAFSRAEAAMIASSE
VEDETTLKVTMVAPVPKYAQAIVTSTLNWIAEPAALEKGQQKFDTQPIGAGPFTLSKWTRQAEMELVRNTRYWDAPKPYL
DRLSLRPATDSSQRYNTVLTGGADLAVESSWVNLDKAKQARLSTNVMQLSGGIFIALNLRSEPFSDIRARQALAAAIDIE
TLNLAAYSGTAEAADTLFSKISPYHSDVPLHTTDHDKAQRLFDELAGEGKPVTFTFKTPPTTENRAVAENIQTQLSTFRN
VKAEVKVIEVAEFSQLRTTHDFDAAASSALFQDPDPRLTTTFAGDSPANLTGIDDAVLNESLQAGRVARTEAEAKDAYVT
VQERLAVVTPVIFTVRAAPSTISSPNVGGIVQYGIGSLLPSELWIKP
>Mature_527_residues
MIRGARLGAVAVTCCAALVLAACGGGESDGSSRTSENGASGATGNPVPGGEGRILLLSEPRSLDPAALNNVYAAGAAVGN
ALYGTLMTNNEAGEIQYSMAESFTTPDNGATFELKLRPGLAFSDGTALTAEAVKYNWDRIKDPKLGAFSRAEAAMIASSE
VEDETTLKVTMVAPVPKYAQAIVTSTLNWIAEPAALEKGQQKFDTQPIGAGPFTLSKWTRQAEMELVRNTRYWDAPKPYL
DRLSLRPATDSSQRYNTVLTGGADLAVESSWVNLDKAKQARLSTNVMQLSGGIFIALNLRSEPFSDIRARQALAAAIDIE
TLNLAAYSGTAEAADTLFSKISPYHSDVPLHTTDHDKAQRLFDELAGEGKPVTFTFKTPPTTENRAVAENIQTQLSTFRN
VKAEVKVIEVAEFSQLRTTHDFDAAASSALFQDPDPRLTTTFAGDSPANLTGIDDAVLNESLQAGRVARTEAEAKDAYVT
VQERLAVVTPVIFTVRAAPSTISSPNVGGIVQYGIGSLLPSELWIKP

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=360, Percent_Identity=29.4444444444444, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789966, Length=352, Percent_Identity=24.7159090909091, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87081878, Length=477, Percent_Identity=25.9958071278826, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787495, Length=497, Percent_Identity=23.5412474849095, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789397, Length=287, Percent_Identity=26.8292682926829, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789887, Length=481, Percent_Identity=24.5322245322245, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787551, Length=268, Percent_Identity=25, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787762, Length=331, Percent_Identity=24.1691842900302, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56125; Mature: 56125

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRGARLGAVAVTCCAALVLAACGGGESDGSSRTSENGASGATGNPVPGGEGRILLLSEP
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
RSLDPAALNNVYAAGAAVGNALYGTLMTNNEAGEIQYSMAESFTTPDNGATFELKLRPGL
CCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEHHHCCCCCCCCCEEEEEECCCC
AFSDGTALTAEAVKYNWDRIKDPKLGAFSRAEAAMIASSEVEDETTLKVTMVAPVPKYAQ
EECCCCEEEHHHHHCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCHHHH
AIVTSTLNWIAEPAALEKGQQKFDTQPIGAGPFTLSKWTRQAEMELVRNTRYWDAPKPYL
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
DRLSLRPATDSSQRYNTVLTGGADLAVESSWVNLDKAKQARLSTNVMQLSGGIFIALNLR
HCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHEEECCCEEEEEEEC
SEPFSDIRARQALAAAIDIETLNLAAYSGTAEAADTLFSKISPYHSDVPLHTTDHDKAQR
CCCHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
LFDELAGEGKPVTFTFKTPPTTENRAVAENIQTQLSTFRNVKAEVKVIEVAEFSQLRTTH
HHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHCCC
DFDAAASSALFQDPDPRLTTTFAGDSPANLTGIDDAVLNESLQAGRVARTEAEAKDAYVT
CHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHEEE
VQERLAVVTPVIFTVRAAPSTISSPNVGGIVQYGIGSLLPSELWIKP
HHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MIRGARLGAVAVTCCAALVLAACGGGESDGSSRTSENGASGATGNPVPGGEGRILLLSEP
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
RSLDPAALNNVYAAGAAVGNALYGTLMTNNEAGEIQYSMAESFTTPDNGATFELKLRPGL
CCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEHHHCCCCCCCCCEEEEEECCCC
AFSDGTALTAEAVKYNWDRIKDPKLGAFSRAEAAMIASSEVEDETTLKVTMVAPVPKYAQ
EECCCCEEEHHHHHCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCHHHH
AIVTSTLNWIAEPAALEKGQQKFDTQPIGAGPFTLSKWTRQAEMELVRNTRYWDAPKPYL
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
DRLSLRPATDSSQRYNTVLTGGADLAVESSWVNLDKAKQARLSTNVMQLSGGIFIALNLR
HCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHEEECCCEEEEEEEC
SEPFSDIRARQALAAAIDIETLNLAAYSGTAEAADTLFSKISPYHSDVPLHTTDHDKAQR
CCCHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
LFDELAGEGKPVTFTFKTPPTTENRAVAENIQTQLSTFRNVKAEVKVIEVAEFSQLRTTH
HHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHCCC
DFDAAASSALFQDPDPRLTTTFAGDSPANLTGIDDAVLNESLQAGRVARTEAEAKDAYVT
CHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHEEE
VQERLAVVTPVIFTVRAAPSTISSPNVGGIVQYGIGSLLPSELWIKP
HHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA