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The map label for this gene is kshB [H]

Identifier: 158314796

GI number: 158314796

Start: 3505136

End: 3506002

Strand: Direct

Name: kshB [H]

Synonym: Franean1_2980

Alternate gene names: 158314796

Gene position: 3505136-3506002 (Clockwise)

Preceding gene: 158314795

Following gene: 158314797

Centisome position: 39.02

GC content: 67.01

Gene sequence:

>867_bases
ATGGACGAGATCGCGGCCCACGCGCAGTGTCGAAGATGGGCACAGAACTTCACCGGGCCAGGCCTCGCACTCGCGGACCG
CGCGCTGCTGCGACCGCGCCTGCAGGCGCCCTTCCTCGTCGGTGATCCGCCCGGCCGACCCTCCACGGCCGCCCAGGATC
CGGGTTTCTACACTCTTCGGGTCGCGCGGGTGGTACGCGAGACCAAGAACACCACATCGTTCTTCCTCGCGATCCCCGAG
GAGTTCGCCGACAGGTTCCGCCACCGGGCCGGCCAGTACATCACCCTTCGCTTCGACCTCGACGCCGAGATCCATCTGCG
CCCCTACTCGATCTCCTCGGTGGCAGGCCGCGACGAACAGATGCGGGTCACCGTGCAGCGGGTGCGCGGCGGTCTCGTGT
CGAACTGGATGAACGACGACCTCGGGCGAGGGGACGAAGTCGTGACGACCGCACCGATGGGTCATTTCACACTGCCCGGA
CTGGGACCCGGCGGATACTCTGTCATCGCGTTTGCCGAGAAAATCGGCATAGCCCCGATCCTCTCCATCGCCGCCACCGC
GCTGCACCAGCCGCTCCCGCGGGTACACGTGCTGTACACGACCGCGGACTCCGCTCCCGCTGTGTTCGACGCAGGACTGC
GAGCACTGGAGCGGGAACACGGAGACCGCCTCACCGTGCGGCGCTGCTACAACGCCCAGGCCAGCCAGCTCCAGGACAGG
GACATTCTCGGCCTCTCGACGGTGACTGAAAGCGAATCGCTGTTCTACGTCTGTGGGTCGGAGCGCTTCACCGACCGCGT
GGAACAGACGCTGCTCGCCGGCGGAACAGCCCCCGACCGGATCCGCGTCGAGCCTTTCACCACCTGA

Upstream 100 bases:

>100_bases
GCCCGTGCCGCAGCACAGCCGGTCGACGGAGCAACGCGGCACGACAGCCAGAACGACGAGGCAGCCCCAGCGCGGCCAGA
GGCATGGGATCAGGAAAACT

Downstream 100 bases:

>100_bases
CCCGGCCCGATTTCCCGCCCACCACCCCAGACAAGATCCGGGCGATGGGCGAGGCAGCCACCATATCCACGGGCTTCCGT
GGGACCAGCAAGACCACCGG

Product: oxidoreductase FAD-binding subunit

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MDEIAAHAQCRRWAQNFTGPGLALADRALLRPRLQAPFLVGDPPGRPSTAAQDPGFYTLRVARVVRETKNTTSFFLAIPE
EFADRFRHRAGQYITLRFDLDAEIHLRPYSISSVAGRDEQMRVTVQRVRGGLVSNWMNDDLGRGDEVVTTAPMGHFTLPG
LGPGGYSVIAFAEKIGIAPILSIAATALHQPLPRVHVLYTTADSAPAVFDAGLRALEREHGDRLTVRRCYNAQASQLQDR
DILGLSTVTESESLFYVCGSERFTDRVEQTLLAGGTAPDRIRVEPFTT

Sequences:

>Translated_288_residues
MDEIAAHAQCRRWAQNFTGPGLALADRALLRPRLQAPFLVGDPPGRPSTAAQDPGFYTLRVARVVRETKNTTSFFLAIPE
EFADRFRHRAGQYITLRFDLDAEIHLRPYSISSVAGRDEQMRVTVQRVRGGLVSNWMNDDLGRGDEVVTTAPMGHFTLPG
LGPGGYSVIAFAEKIGIAPILSIAATALHQPLPRVHVLYTTADSAPAVFDAGLRALEREHGDRLTVRRCYNAQASQLQDR
DILGLSTVTESESLFYVCGSERFTDRVEQTLLAGGTAPDRIRVEPFTT
>Mature_288_residues
MDEIAAHAQCRRWAQNFTGPGLALADRALLRPRLQAPFLVGDPPGRPSTAAQDPGFYTLRVARVVRETKNTTSFFLAIPE
EFADRFRHRAGQYITLRFDLDAEIHLRPYSISSVAGRDEQMRVTVQRVRGGLVSNWMNDDLGRGDEVVTTAPMGHFTLPG
LGPGGYSVIAFAEKIGIAPILSIAATALHQPLPRVHVLYTTADSAPAVFDAGLRALEREHGDRLTVRRCYNAQASQLQDR
DILGLSTVTESESLFYVCGSERFTDRVEQTLLAGGTAPDRIRVEPFTT

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=246, Percent_Identity=27.6422764227642, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787098, Length=154, Percent_Identity=29.2207792207792, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 31733; Mature: 31733

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEIAAHAQCRRWAQNFTGPGLALADRALLRPRLQAPFLVGDPPGRPSTAAQDPGFYTLR
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCEEHHH
VARVVRETKNTTSFFLAIPEEFADRFRHRAGQYITLRFDLDAEIHLRPYSISSVAGRDEQ
HHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCHHHCCCCHH
MRVTVQRVRGGLVSNWMNDDLGRGDEVVTTAPMGHFTLPGLGPGGYSVIAFAEKIGIAPI
HHHHHHHHHCHHHHHHHCCCCCCCCCEEEECCCCCEECCCCCCCCHHHHHHHHHHCCHHH
LSIAATALHQPLPRVHVLYTTADSAPAVFDAGLRALEREHGDRLTVRRCYNAQASQLQDR
HHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEHHHHCCHHHHCCCC
DILGLSTVTESESLFYVCGSERFTDRVEQTLLAGGTAPDRIRVEPFTT
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCC
>Mature Secondary Structure
MDEIAAHAQCRRWAQNFTGPGLALADRALLRPRLQAPFLVGDPPGRPSTAAQDPGFYTLR
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCEEHHH
VARVVRETKNTTSFFLAIPEEFADRFRHRAGQYITLRFDLDAEIHLRPYSISSVAGRDEQ
HHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCHHHCCCCHH
MRVTVQRVRGGLVSNWMNDDLGRGDEVVTTAPMGHFTLPGLGPGGYSVIAFAEKIGIAPI
HHHHHHHHHCHHHHHHHCCCCCCCCCEEEECCCCCEECCCCCCCCHHHHHHHHHHCCHHH
LSIAATALHQPLPRVHVLYTTADSAPAVFDAGLRALEREHGDRLTVRRCYNAQASQLQDR
HHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEHHHHCCHHHHCCCC
DILGLSTVTESESLFYVCGSERFTDRVEQTLLAGGTAPDRIRVEPFTT
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA