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The map label for this gene is hcaE [H]

Identifier: 158314792

GI number: 158314792

Start: 3501695

End: 3502954

Strand: Direct

Name: hcaE [H]

Synonym: Franean1_2976

Alternate gene names: 158314792

Gene position: 3501695-3502954 (Clockwise)

Preceding gene: 158314791

Following gene: 158314793

Centisome position: 38.99

GC content: 64.76

Gene sequence:

>1260_bases
ATGCCGCGTTTCCCGAAACCACCGGAAGGCAGCTGGACACAGCACTATCCCGCCCTCGGGACGGAACCGGTGTCCTACGA
GGACTCCATCTCCCCCGAGTACTACGAGCGCGAACGGAAGGCCGTTTTCAAGCGGGCGTGGTTAAACGTCGGCAGAGCCG
AGCAGCTCCCGCGGGTCGGTAGCTACTTCACGAAGGACGTCCACGCCGCCCACGCCTCGATCATCGTGGTGCGCGGGAAG
AGCGGGCAGGTCAACGCCTTCCACAACATCTGCCGCCACCGGGGCAACAAGCTCGCGTGGACCGACGACCCGAAACGGGA
GACGCAGGGCGTCTGCCGGCAGTTCACCTGCAAGTACCACGGCTGGCGCTACGATCTCGACGGTGCGCTGACCTTCGTCC
AGCAGGAGGGCGAGTTCTTCGACCTGGACAAGGCCAGCAACGGCCTGGCGCCGGTGCACTGCGAGGTCTGGGCCGGGTTC
ATCTTCGTGAACTTCGCCCGGCAGCCGGAGCAGACGCTGCGCGAGTTCCTCGGGCCGATGGTGACGGACCTGGAGGGCTA
CCCGTTCGAGAAGCAGACCGCGCGCTTCGGGTACAGCACCACCATCCAGGCGAACTGGAAGCTGTACATGGATGCGTTCG
CGGAGTTCTACCACGCACCGGTGCTGCACGCGAAGCAGTCGCCGGAGAAGTACTCCGCGGCCGCCCAGCAGGCCGGTTTC
GAGGCGCCGCACTACCAGATCGAGGGGCCGCACCGGCTGGTCAGCACGTCCGGTGTCCGGTTCTGGGAGATGGACGCCGA
GCTGCTCAAGCCGACCGAGATCCTGACCCGCAGTGGTCTTTTCGGCCCGTGGGACAAATACGACCTGGGTAAGATGCCGC
GCGGGGTCAACCCGGCGGGCTGCGACCCCTGGGGCCTCGACTCCTTCCAGCTCTTCCCGAACTTCGTGATCCTCATCTGG
AGCCAGGGCTGGTACCTCACCTACCACTACTGGCCGACGTCCTATAACACACACGTGTTCGAGGGCAACGTCTACTTCCT
GCCGGCCAGAAACATCCGTGAGCGGATCGCGCAGGAGATGGCGGCGGTGACCTTCAAGGAGTTCGCGTTGCAGGACTCGA
ACACCCTCGAGGCGACACAGATGATGCTGGAGACCGGGTACGTCGACCGGTTCCACCTCAACGACCAGGAAATCCTGTGC
CGGCATCTGCACGTCGCGGTGCGCGACTGGGTGAACAACCACGAGGGGGCGCGTTCATGA

Upstream 100 bases:

>100_bases
GCTTCTCGCCCCGCGAAACCCCGGCGCGGTACACGCAGAATCCCGGGTCGAACCGGGTAGCCCGTCTGTTCCATGACCGC
ATCACCACAGGAGGTGGACC

Downstream 100 bases:

>100_bases
GCCACCTGCTACCCGCCGAGTTCGCCGACCTCGAGCCGTTCGCGGTGAAATGGTGCCTGCCCACCGAGCACGAGCGGTAC
AACACCCGGCTCGCCAGCTC

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPRFPKPPEGSWTQHYPALGTEPVSYEDSISPEYYERERKAVFKRAWLNVGRAEQLPRVGSYFTKDVHAAHASIIVVRGK
SGQVNAFHNICRHRGNKLAWTDDPKRETQGVCRQFTCKYHGWRYDLDGALTFVQQEGEFFDLDKASNGLAPVHCEVWAGF
IFVNFARQPEQTLREFLGPMVTDLEGYPFEKQTARFGYSTTIQANWKLYMDAFAEFYHAPVLHAKQSPEKYSAAAQQAGF
EAPHYQIEGPHRLVSTSGVRFWEMDAELLKPTEILTRSGLFGPWDKYDLGKMPRGVNPAGCDPWGLDSFQLFPNFVILIW
SQGWYLTYHYWPTSYNTHVFEGNVYFLPARNIRERIAQEMAAVTFKEFALQDSNTLEATQMMLETGYVDRFHLNDQEILC
RHLHVAVRDWVNNHEGARS

Sequences:

>Translated_419_residues
MPRFPKPPEGSWTQHYPALGTEPVSYEDSISPEYYERERKAVFKRAWLNVGRAEQLPRVGSYFTKDVHAAHASIIVVRGK
SGQVNAFHNICRHRGNKLAWTDDPKRETQGVCRQFTCKYHGWRYDLDGALTFVQQEGEFFDLDKASNGLAPVHCEVWAGF
IFVNFARQPEQTLREFLGPMVTDLEGYPFEKQTARFGYSTTIQANWKLYMDAFAEFYHAPVLHAKQSPEKYSAAAQQAGF
EAPHYQIEGPHRLVSTSGVRFWEMDAELLKPTEILTRSGLFGPWDKYDLGKMPRGVNPAGCDPWGLDSFQLFPNFVILIW
SQGWYLTYHYWPTSYNTHVFEGNVYFLPARNIRERIAQEMAAVTFKEFALQDSNTLEATQMMLETGYVDRFHLNDQEILC
RHLHVAVRDWVNNHEGARS
>Mature_418_residues
PRFPKPPEGSWTQHYPALGTEPVSYEDSISPEYYERERKAVFKRAWLNVGRAEQLPRVGSYFTKDVHAAHASIIVVRGKS
GQVNAFHNICRHRGNKLAWTDDPKRETQGVCRQFTCKYHGWRYDLDGALTFVQQEGEFFDLDKASNGLAPVHCEVWAGFI
FVNFARQPEQTLREFLGPMVTDLEGYPFEKQTARFGYSTTIQANWKLYMDAFAEFYHAPVLHAKQSPEKYSAAAQQAGFE
APHYQIEGPHRLVSTSGVRFWEMDAELLKPTEILTRSGLFGPWDKYDLGKMPRGVNPAGCDPWGLDSFQLFPNFVILIWS
QGWYLTYHYWPTSYNTHVFEGNVYFLPARNIRERIAQEMAAVTFKEFALQDSNTLEATQMMLETGYVDRFHLNDQEILCR
HLHVAVRDWVNNHEGARS

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788103, Length=192, Percent_Identity=30.7291666666667, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788888, Length=210, Percent_Identity=33.8095238095238, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 48385; Mature: 48254

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFPKPPEGSWTQHYPALGTEPVSYEDSISPEYYERERKAVFKRAWLNVGRAEQLPRVG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHH
SYFTKDVHAAHASIIVVRGKSGQVNAFHNICRHRGNKLAWTDDPKRETQGVCRQFTCKYH
HHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GWRYDLDGALTFVQQEGEFFDLDKASNGLAPVHCEVWAGFIFVNFARQPEQTLREFLGPM
CEEEECCCEEEEEECCCCEEECCCCCCCCCEEEHHHHEEEHEEECCCCHHHHHHHHHCHH
VTDLEGYPFEKQTARFGYSTTIQANWKLYMDAFAEFYHAPVLHAKQSPEKYSAAAQQAGF
HHCCCCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHCCC
EAPHYQIEGPHRLVSTSGVRFWEMDAELLKPTEILTRSGLFGPWDKYDLGKMPRGVNPAG
CCCCEEECCCCEEHCCCCCEEEECCHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
CDPWGLDSFQLFPNFVILIWSQGWYLTYHYWPTSYNTHVFEGNVYFLPARNIRERIAQEM
CCCCCCCHHHHCCCEEEEEECCCEEEEEEEECCCCCCEEEECCEEEEECHHHHHHHHHHH
AAVTFKEFALQDSNTLEATQMMLETGYVDRFHLNDQEILCRHLHVAVRDWVNNHEGARS
HHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PRFPKPPEGSWTQHYPALGTEPVSYEDSISPEYYERERKAVFKRAWLNVGRAEQLPRVG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHH
SYFTKDVHAAHASIIVVRGKSGQVNAFHNICRHRGNKLAWTDDPKRETQGVCRQFTCKYH
HHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GWRYDLDGALTFVQQEGEFFDLDKASNGLAPVHCEVWAGFIFVNFARQPEQTLREFLGPM
CEEEECCCEEEEEECCCCEEECCCCCCCCCEEEHHHHEEEHEEECCCCHHHHHHHHHCHH
VTDLEGYPFEKQTARFGYSTTIQANWKLYMDAFAEFYHAPVLHAKQSPEKYSAAAQQAGF
HHCCCCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHCCC
EAPHYQIEGPHRLVSTSGVRFWEMDAELLKPTEILTRSGLFGPWDKYDLGKMPRGVNPAG
CCCCEEECCCCEEHCCCCCEEEECCHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
CDPWGLDSFQLFPNFVILIWSQGWYLTYHYWPTSYNTHVFEGNVYFLPARNIRERIAQEM
CCCCCCCHHHHCCCEEEEEECCCEEEEEEEECCCCCCEEEECCEEEEECHHHHHHHHHHH
AAVTFKEFALQDSNTLEATQMMLETGYVDRFHLNDQEILCRHLHVAVRDWVNNHEGARS
HHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]