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The map label for this gene is kshB [H]

Identifier: 158314789

GI number: 158314789

Start: 3499216

End: 3500241

Strand: Direct

Name: kshB [H]

Synonym: Franean1_2973

Alternate gene names: 158314789

Gene position: 3499216-3500241 (Clockwise)

Preceding gene: 158314787

Following gene: 158314790

Centisome position: 38.96

GC content: 70.08

Gene sequence:

>1026_bases
ATGTCGACAGACACGCTCGCAGCACGTCACCGCTACCACCCCCTGCGGGTGCTCCGCCTCGTCCGGGAGACCTCCGAGGC
CCGCTCGATCGTCTTCGAGGTGCCGTCCGAGCTGACCGAGGTCTTCGCCTACGAGGCGGGCCAGTTCGTCACGCTCCGCG
TCACCCTCGACGGTGAGACCCACGTGCGCAGCTATTCGATGTCGTCGTCGCCGCTGCTGGACGGCGAGCTGCGCGTGACA
GTGAAGCGGGTGCCCGGTGGCCTGATCTCGAACTGGCTGAACGACACGCTCGGCGAGGGCGATGTCCTGGAGGTCAACCC
GCCCGGGGGATCGTTCAGCCTCGACCGCGGCGACCACGACATCGTCGCGTTCGCCGCCGGCAGCGGGATCACCCCCGTGT
TCTCGATCGTCAGGACGGCGCTGGCCACCTCCCACCGGCGCATCCGGGTGCTCTACGCCAACCGCGACCGGGCCGCCGCG
ATCTTCGGCGACGAGCTGGACGCACTGGCGGCGCAGTACCCGGGCCGCCTCACCGTCGAGCACCACGAGGATCTCGTCCG
CGGGTTCGTCCACCGTGACCAGGTGGACCGCGCCGCCGGCGATGTCGAGACCGCCGCGTACTACATCTGCGGGCCTGGCG
GCTTCATGGACGTCGTCGAAGCCGGGCTCCGTGACCTGGGAGCCGACCCGTCGAGGGTGCACATCGAACGGTTCACCCCG
GCAGAGGAGCCGCCGCCCGCCGGTCCCGCCGACGACATCACCGTGACCATCAAGCTCGGCGGGAAGACGGTGACGACCGC
GCACCGCCGTGACAGCACGCTCCTGCAGACGGCCCGGTACGCCGGCCTACGGGCCCCCTCGTCCTGCGAGACGGGCAGCT
GCGCCACTTGCATGGCGCGGGTCGTGCAGGGACGAGCCGAGATGCGGATTAACGACGCCCTGACCCCCGACGAGGTCGCC
GAAGGCTGGGTGCTCACGTGCCAGGCCATGCCGGTGACCCCGGTCATCGAAGTGGTCTACGAATGA

Upstream 100 bases:

>100_bases
GACGTTAGTCATACGAAGAACAGGTGTTGGTCGTTGGCCGACAGCTTGATCCGAACAGGAGGAGGCGTTGCCGGGCGACA
CCAGCGCGATGGGGGAGACG

Downstream 100 bases:

>100_bases
GGAAGGACGAAGCGATGCCGCGTACCAAGGCGCAGCTGTCTCGGGTGGCCGTGGTCACCGGCGGGGCGTCGGGCATAGGC
GAGGCCACCTGCCACCACCT

Product: oxidoreductase FAD-binding subunit

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSTDTLAARHRYHPLRVLRLVRETSEARSIVFEVPSELTEVFAYEAGQFVTLRVTLDGETHVRSYSMSSSPLLDGELRVT
VKRVPGGLISNWLNDTLGEGDVLEVNPPGGSFSLDRGDHDIVAFAAGSGITPVFSIVRTALATSHRRIRVLYANRDRAAA
IFGDELDALAAQYPGRLTVEHHEDLVRGFVHRDQVDRAAGDVETAAYYICGPGGFMDVVEAGLRDLGADPSRVHIERFTP
AEEPPPAGPADDITVTIKLGGKTVTTAHRRDSTLLQTARYAGLRAPSSCETGSCATCMARVVQGRAEMRINDALTPDEVA
EGWVLTCQAMPVTPVIEVVYE

Sequences:

>Translated_341_residues
MSTDTLAARHRYHPLRVLRLVRETSEARSIVFEVPSELTEVFAYEAGQFVTLRVTLDGETHVRSYSMSSSPLLDGELRVT
VKRVPGGLISNWLNDTLGEGDVLEVNPPGGSFSLDRGDHDIVAFAAGSGITPVFSIVRTALATSHRRIRVLYANRDRAAA
IFGDELDALAAQYPGRLTVEHHEDLVRGFVHRDQVDRAAGDVETAAYYICGPGGFMDVVEAGLRDLGADPSRVHIERFTP
AEEPPPAGPADDITVTIKLGGKTVTTAHRRDSTLLQTARYAGLRAPSSCETGSCATCMARVVQGRAEMRINDALTPDEVA
EGWVLTCQAMPVTPVIEVVYE
>Mature_340_residues
STDTLAARHRYHPLRVLRLVRETSEARSIVFEVPSELTEVFAYEAGQFVTLRVTLDGETHVRSYSMSSSPLLDGELRVTV
KRVPGGLISNWLNDTLGEGDVLEVNPPGGSFSLDRGDHDIVAFAAGSGITPVFSIVRTALATSHRRIRVLYANRDRAAAI
FGDELDALAAQYPGRLTVEHHEDLVRGFVHRDQVDRAAGDVETAAYYICGPGGFMDVVEAGLRDLGADPSRVHIERFTPA
EEPPPAGPADDITVTIKLGGKTVTTAHRRDSTLLQTARYAGLRAPSSCETGSCATCMARVVQGRAEMRINDALTPDEVAE
GWVLTCQAMPVTPVIEVVYE

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=351, Percent_Identity=33.048433048433, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1787098, Length=330, Percent_Identity=28.1818181818182, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788903, Length=249, Percent_Identity=29.3172690763052, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788104, Length=292, Percent_Identity=24.6575342465753, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323510, Length=192, Percent_Identity=30.2083333333333, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322699, Length=234, Percent_Identity=23.5042735042735, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322146, Length=205, Percent_Identity=23.9024390243902, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37012; Mature: 36881

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDTLAARHRYHPLRVLRLVRETSEARSIVFEVPSELTEVFAYEAGQFVTLRVTLDGET
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHEEHHCHHHHHHHHHHCCCCEEEEEEEECCCC
HVRSYSMSSSPLLDGELRVTVKRVPGGLISNWLNDTLGEGDVLEVNPPGGSFSLDRGDHD
EEEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCEECCCCCCE
IVAFAAGSGITPVFSIVRTALATSHRRIRVLYANRDRAAAIFGDELDALAAQYPGRLTVE
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCHHHHHHHHCCCCEEEH
HHEDLVRGFVHRDQVDRAAGDVETAAYYICGPGGFMDVVEAGLRDLGADPSRVHIERFTP
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCC
AEEPPPAGPADDITVTIKLGGKTVTTAHRRDSTLLQTARYAGLRAPSSCETGSCATCMAR
CCCCCCCCCCCCEEEEEEECCEEEEECHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VVQGRAEMRINDALTPDEVAEGWVLTCQAMPVTPVIEVVYE
HHCCHHHEEECCCCCHHHHCCCEEEEEECCCCCHHHHHCCC
>Mature Secondary Structure 
STDTLAARHRYHPLRVLRLVRETSEARSIVFEVPSELTEVFAYEAGQFVTLRVTLDGET
CCCHHHHHHCCCHHHHHHHHHHHHHHHHEEHHCHHHHHHHHHHCCCCEEEEEEEECCCC
HVRSYSMSSSPLLDGELRVTVKRVPGGLISNWLNDTLGEGDVLEVNPPGGSFSLDRGDHD
EEEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCEECCCCCCE
IVAFAAGSGITPVFSIVRTALATSHRRIRVLYANRDRAAAIFGDELDALAAQYPGRLTVE
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCHHHHHHHHCCCCEEEH
HHEDLVRGFVHRDQVDRAAGDVETAAYYICGPGGFMDVVEAGLRDLGADPSRVHIERFTP
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCC
AEEPPPAGPADDITVTIKLGGKTVTTAHRRDSTLLQTARYAGLRAPSSCETGSCATCMAR
CCCCCCCCCCCCEEEEEEECCEEEEECHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
VVQGRAEMRINDALTPDEVAEGWVLTCQAMPVTPVIEVVYE
HHCCHHHEEECCCCCHHHHCCCEEEEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA