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The map label for this gene is hcaE [H]

Identifier: 158314785

GI number: 158314785

Start: 3495909

End: 3497198

Strand: Direct

Name: hcaE [H]

Synonym: Franean1_2969

Alternate gene names: 158314785

Gene position: 3495909-3497198 (Clockwise)

Preceding gene: 158314781

Following gene: 158314786

Centisome position: 38.92

GC content: 62.79

Gene sequence:

>1290_bases
ATGGCGCGTCTCGCCAAGCCGTCGGAGGCCAGCTGGACCGAGCGCTACCCGGAGCTGGGCACCGGCCTCGTGTCGTACGA
GGACTCCATCTCCCCGGAGTTCTACGAGCTCGAGCGCGAGGCGATCTTCCGGCGTGCCTGGCTCAATGTCGGCCGGGTCG
AACAGTTGCCCCGCAAAGGGAGCTTCTTCACGAAGGAGCTTGCCGTTGCCAAGACGTCGCTCATCGTCACGCGCGATCTC
GATGGACAGGTTCGCGCCTTCCACAACGTGTGCCGCCACCGCGGCAACAAGCTGGTGTGGGACAAGACGCCGAGGGACGA
GACCAGTGGTGTCTGTCGCCAGTTCATGTGCAAGTACCACGGCTGGCGGTACGGCCTGGACGGTCAGCTGAAGTTCGTCC
TTCAGGAGGAGGAGTTCTTCGACTTCGACAAGGCGGACTTCCCGCTTGTGTCAGCTCACTGCGAGGTCTGGGAAGGCTTC
ATCTTCATCAACCTGTCGGACGAACCCAGCCAGTCGCTCCTTGAATTCCTTGGGCCGATGGTGCGCGGGCTCGAGGGCTA
CCCGTTCCATCACGTCACCGAGCGGTATGCCTTCAAGGCGGACATCCTCAGTAACTGGAAGATCTACCTTGACGCATTCC
AGGAGTACTATCATGCGTCGATCTTGCACTCGCAGCAGCAGGTGCCGAGCCTGCGCAGCTTTGAGTCCGGTTTCAAGGCG
CCGCACTACCAGGTCGATGGACCGCATCGGCTGGTGAGCACCGGCGGCTGGAAGGGCGTGCCGCGGCACATGCTGCCGCT
CGACCAGATGTACCCGATCGAGCACAACATCGAGGCCGGCATGATGGGCCCCTGGCAGCGCCCGGACATCCCCGAGCTCG
ACCCGGCCAACCTGCCGGCGGGGCTCAACCCTGGCGGGCTCGACCCCTGGTCGATCTCGAACTTCCAGATCTGGCCCAAT
TTCGTGATCCTGGTCTATGAGCGGGGCTGGTACCTCACCTACCAGTACTGGCCGACTTCCCACAACACGCATGTGTGGGA
GATGTCGTACTACTTCCCGCCGTCGCGGAATGCCAGCGAACGGATCCGGCACGAGGTCACCGCCGTCGTGTCCAAGGAGG
CCGGTCTCCAGGACGCGGGCACCCTCGACGGCACCCAGATGGGCCTGGAATCCAGGGTTATCGACAGATATCCGCTGTCC
GATCAGGAGATTACCGTACGTCACCTGCACAAGGTCACCGGAGATTGGGTGCAGTCCTATCTTCGGGACGGAAAGGCAGT
CCGGGCATGA

Upstream 100 bases:

>100_bases
TTGCGGCCCCGGCAGCTCGTATGTTAGTCACTTGGGGAACAGTTAGTCGGTTGGGGAACACCACCAGCGATCTTGAGGGA
CCCGAACGGGAGGATGAGCC

Downstream 100 bases:

>100_bases
GTGACCCGTTACTCCCCGAGAAGTTCGCCGACCTCGAGCCCTTTGTGAAAAGGTGGTGCATTCCAACCGAGGCCGCCCGG
TACGCCGAGCGACTGAACAG

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MARLAKPSEASWTERYPELGTGLVSYEDSISPEFYELEREAIFRRAWLNVGRVEQLPRKGSFFTKELAVAKTSLIVTRDL
DGQVRAFHNVCRHRGNKLVWDKTPRDETSGVCRQFMCKYHGWRYGLDGQLKFVLQEEEFFDFDKADFPLVSAHCEVWEGF
IFINLSDEPSQSLLEFLGPMVRGLEGYPFHHVTERYAFKADILSNWKIYLDAFQEYYHASILHSQQQVPSLRSFESGFKA
PHYQVDGPHRLVSTGGWKGVPRHMLPLDQMYPIEHNIEAGMMGPWQRPDIPELDPANLPAGLNPGGLDPWSISNFQIWPN
FVILVYERGWYLTYQYWPTSHNTHVWEMSYYFPPSRNASERIRHEVTAVVSKEAGLQDAGTLDGTQMGLESRVIDRYPLS
DQEITVRHLHKVTGDWVQSYLRDGKAVRA

Sequences:

>Translated_429_residues
MARLAKPSEASWTERYPELGTGLVSYEDSISPEFYELEREAIFRRAWLNVGRVEQLPRKGSFFTKELAVAKTSLIVTRDL
DGQVRAFHNVCRHRGNKLVWDKTPRDETSGVCRQFMCKYHGWRYGLDGQLKFVLQEEEFFDFDKADFPLVSAHCEVWEGF
IFINLSDEPSQSLLEFLGPMVRGLEGYPFHHVTERYAFKADILSNWKIYLDAFQEYYHASILHSQQQVPSLRSFESGFKA
PHYQVDGPHRLVSTGGWKGVPRHMLPLDQMYPIEHNIEAGMMGPWQRPDIPELDPANLPAGLNPGGLDPWSISNFQIWPN
FVILVYERGWYLTYQYWPTSHNTHVWEMSYYFPPSRNASERIRHEVTAVVSKEAGLQDAGTLDGTQMGLESRVIDRYPLS
DQEITVRHLHKVTGDWVQSYLRDGKAVRA
>Mature_428_residues
ARLAKPSEASWTERYPELGTGLVSYEDSISPEFYELEREAIFRRAWLNVGRVEQLPRKGSFFTKELAVAKTSLIVTRDLD
GQVRAFHNVCRHRGNKLVWDKTPRDETSGVCRQFMCKYHGWRYGLDGQLKFVLQEEEFFDFDKADFPLVSAHCEVWEGFI
FINLSDEPSQSLLEFLGPMVRGLEGYPFHHVTERYAFKADILSNWKIYLDAFQEYYHASILHSQQQVPSLRSFESGFKAP
HYQVDGPHRLVSTGGWKGVPRHMLPLDQMYPIEHNIEAGMMGPWQRPDIPELDPANLPAGLNPGGLDPWSISNFQIWPNF
VILVYERGWYLTYQYWPTSHNTHVWEMSYYFPPSRNASERIRHEVTAVVSKEAGLQDAGTLDGTQMGLESRVIDRYPLSD
QEITVRHLHKVTGDWVQSYLRDGKAVRA

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788103, Length=190, Percent_Identity=28.9473684210526, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788888, Length=217, Percent_Identity=30.4147465437788, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 49654; Mature: 49522

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLAKPSEASWTERYPELGTGLVSYEDSISPEFYELEREAIFRRAWLNVGRVEQLPRKG
CCCCCCCCCCCHHHHCHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCC
SFFTKELAVAKTSLIVTRDLDGQVRAFHNVCRHRGNKLVWDKTPRDETSGVCRQFMCKYH
CHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHC
GWRYGLDGQLKFVLQEEEFFDFDKADFPLVSAHCEVWEGFIFINLSDEPSQSLLEFLGPM
CEEECCCCEEEEEEEHHHHCCCCCCCCCEEEHHHHEECCEEEEEECCCCHHHHHHHHHHH
VRGLEGYPFHHVTERYAFKADILSNWKIYLDAFQEYYHASILHSQQQVPSLRSFESGFKA
HHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PHYQVDGPHRLVSTGGWKGVPRHMLPLDQMYPIEHNIEAGMMGPWQRPDIPELDPANLPA
CCEECCCCCEEEECCCCCCCCHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLNPGGLDPWSISNFQIWPNFVILVYERGWYLTYQYWPTSHNTHVWEMSYYFPPSRNASE
CCCCCCCCCCCCCCEEECCCEEEEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCHHH
RIRHEVTAVVSKEAGLQDAGTLDGTQMGLESRVIDRYPLSDQEITVRHLHKVTGDWVQSY
HHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LRDGKAVRA
HHCCCCCCC
>Mature Secondary Structure 
ARLAKPSEASWTERYPELGTGLVSYEDSISPEFYELEREAIFRRAWLNVGRVEQLPRKG
CCCCCCCCCCHHHHCHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCC
SFFTKELAVAKTSLIVTRDLDGQVRAFHNVCRHRGNKLVWDKTPRDETSGVCRQFMCKYH
CHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHC
GWRYGLDGQLKFVLQEEEFFDFDKADFPLVSAHCEVWEGFIFINLSDEPSQSLLEFLGPM
CEEECCCCEEEEEEEHHHHCCCCCCCCCEEEHHHHEECCEEEEEECCCCHHHHHHHHHHH
VRGLEGYPFHHVTERYAFKADILSNWKIYLDAFQEYYHASILHSQQQVPSLRSFESGFKA
HHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PHYQVDGPHRLVSTGGWKGVPRHMLPLDQMYPIEHNIEAGMMGPWQRPDIPELDPANLPA
CCEECCCCCEEEECCCCCCCCHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLNPGGLDPWSISNFQIWPNFVILVYERGWYLTYQYWPTSHNTHVWEMSYYFPPSRNASE
CCCCCCCCCCCCCCEEECCCEEEEEEECCEEEEEEEECCCCCCEEEEEEEECCCCCCHHH
RIRHEVTAVVSKEAGLQDAGTLDGTQMGLESRVIDRYPLSDQEITVRHLHKVTGDWVQSY
HHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LRDGKAVRA
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]