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The map label for this gene is gsiB [H]

Identifier: 158314340

GI number: 158314340

Start: 2988527

End: 2990086

Strand: Direct

Name: gsiB [H]

Synonym: Franean1_2510

Alternate gene names: 158314340

Gene position: 2988527-2990086 (Clockwise)

Preceding gene: 158314336

Following gene: 158314341

Centisome position: 33.27

GC content: 69.29

Gene sequence:

>1560_bases
GTGAACGCACAACTCCGAGCCGATTCGACGCTAATGGGCCGCCGTGGCTTCCTCGGTCTCGGCGTGCTCGCTGGCTCCTC
CCTGCTGCTCGCCGCCTGCGGCGACGACAGCGGTTCGGTCTCGTCCGCCGGCAAGGAGGGCGGGACGTTGCGGTGGGGCT
GGTCCGCGGTCACCTCCTGGGACCCGGTGACCTCGTCCGCGGGCTGGGACGTGCACGCGCTTTCATTGGCCTACGCCGCC
CTCACCAAGCTCGACGAGAAGGGCAACCCGGTACCGGCGCTGGCCGAGTCGTGGAAGTACAACGCGGACGGCACCCAGGT
CGCCTTCACTCTGCGGGCCGGCCTGACGTTCAGCGACGGCACGCCGCTGAACGCCACCGCGGTCAGCAAGAGCCTCGCGC
GGGGCCGGGACTTCGCCGGGTCGCTCGTCGCCGCGCAGCTGGCCAACGTGAAGACGTTGGCCGCGGACGACGACGCCCGC
ACCGTCACCATCGGACTGGCCGCCACGGACTACCAGATCCCGAGCCTGCTCGCCGGCAAGACCGGCATGATCGTCAGTCC
GACGGCCTTCGAGAAGGACGCGAAGGGGCTGGCCACCAAGCCGGTAGGGGCCGGGCCGTTCCGGCTCACGGAGTACGTGC
CGAACCAGTCGGCGAAGCTCGTCCGGTTCCCCGAGTACTGGGACAAGGCGAACATCCACCTCGACGCCTTCGAGCTGTAC
CCGGCGCCCGAGGCGGCGACGGCGGTCCCGGCGCTGCAGTCCGGGCGGCTCGACGTCGCCCAGATCCCGGGCAGCCAGGT
CGAGGCGGCGAAGGCCGCGGGCCTTGAGGTGCAGATCATCCCCTCGCTGGTGACGACGGTGCTCGACGTGAACATCACGA
TGAAGCCGTTCGACAACCCGAAGGTCGTCGAGGCGTTCAAGCACGCCCTCGACCGCAAGGCGCTCGCCGACACCCAGACC
TTCGGGCTGGGCGTGGTCAACTACCAGCCGTTCCCGCCGGGGTACATCGGCCACGAACCGAGCCTGGAGAACGCGTTTCC
GTACGACCCGGAGAAGGCGAAGAAGCTGCTCGCCGAGGCCGGGTTCCCGGACGGAGTGGAGGTGCCGCTGACCACCACGG
GCGCCAGCTCCGCTCTTGCCGAGCAGGTGCAGGCGCAGCTCGCCAAGGTCGGCGTGAAGATCACCATCGAGACGATCCCG
GCGGCGCAGGCCACCCAGATCATGTACATCCAGCACTCGAGGGCGCTGGCCACGGACGGCTTCGCAGGTCGTGACTCGGC
CGTGCAGGCCTTCCAGGTGCTGTTCGGCGAGCAGGGCCTGATGAACCCCGGCCGGCAGACGCCCCCCGAACTGACCGCGG
CGCTGCAGAAGGTGCGGGAGACGCCGTTGGACGATCCGTCGTATCCGACGGTGCTCCGGGCCGCCACGAAGATCGCGGTC
GAGAAGATGCCGAACATCTTCCTCTTCACCACGCCGCGCGTTCTCGCCCGCAAGAAGAACGTCTCGGAGCTGGGCAGCTA
CCTGGCGGTACAGCGCTTCGAGGGCGTCCGGGTCGGGTAA

Upstream 100 bases:

>100_bases
GAACGTCGGTGACCGCTGATCGCGGTCTGCCCGCGTGCCGCTACGGGCCACGCCTTCTATTCCGCGTACCCACGTTTATG
CGAAAGGACAACCGGCCCCC

Downstream 100 bases:

>100_bases
TGCCAGCCACCGACGGTGAGGGCTCCAGATGACGACACTCGAGATCAGCCCGGCGGTCGAGCCGGCCGGCGCGGCGGCTG
ACGCCCCGGCCGGCCGGCGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MNAQLRADSTLMGRRGFLGLGVLAGSSLLLAACGDDSGSVSSAGKEGGTLRWGWSAVTSWDPVTSSAGWDVHALSLAYAA
LTKLDEKGNPVPALAESWKYNADGTQVAFTLRAGLTFSDGTPLNATAVSKSLARGRDFAGSLVAAQLANVKTLAADDDAR
TVTIGLAATDYQIPSLLAGKTGMIVSPTAFEKDAKGLATKPVGAGPFRLTEYVPNQSAKLVRFPEYWDKANIHLDAFELY
PAPEAATAVPALQSGRLDVAQIPGSQVEAAKAAGLEVQIIPSLVTTVLDVNITMKPFDNPKVVEAFKHALDRKALADTQT
FGLGVVNYQPFPPGYIGHEPSLENAFPYDPEKAKKLLAEAGFPDGVEVPLTTTGASSALAEQVQAQLAKVGVKITIETIP
AAQATQIMYIQHSRALATDGFAGRDSAVQAFQVLFGEQGLMNPGRQTPPELTAALQKVRETPLDDPSYPTVLRAATKIAV
EKMPNIFLFTTPRVLARKKNVSELGSYLAVQRFEGVRVG

Sequences:

>Translated_519_residues
MNAQLRADSTLMGRRGFLGLGVLAGSSLLLAACGDDSGSVSSAGKEGGTLRWGWSAVTSWDPVTSSAGWDVHALSLAYAA
LTKLDEKGNPVPALAESWKYNADGTQVAFTLRAGLTFSDGTPLNATAVSKSLARGRDFAGSLVAAQLANVKTLAADDDAR
TVTIGLAATDYQIPSLLAGKTGMIVSPTAFEKDAKGLATKPVGAGPFRLTEYVPNQSAKLVRFPEYWDKANIHLDAFELY
PAPEAATAVPALQSGRLDVAQIPGSQVEAAKAAGLEVQIIPSLVTTVLDVNITMKPFDNPKVVEAFKHALDRKALADTQT
FGLGVVNYQPFPPGYIGHEPSLENAFPYDPEKAKKLLAEAGFPDGVEVPLTTTGASSALAEQVQAQLAKVGVKITIETIP
AAQATQIMYIQHSRALATDGFAGRDSAVQAFQVLFGEQGLMNPGRQTPPELTAALQKVRETPLDDPSYPTVLRAATKIAV
EKMPNIFLFTTPRVLARKKNVSELGSYLAVQRFEGVRVG
>Mature_519_residues
MNAQLRADSTLMGRRGFLGLGVLAGSSLLLAACGDDSGSVSSAGKEGGTLRWGWSAVTSWDPVTSSAGWDVHALSLAYAA
LTKLDEKGNPVPALAESWKYNADGTQVAFTLRAGLTFSDGTPLNATAVSKSLARGRDFAGSLVAAQLANVKTLAADDDAR
TVTIGLAATDYQIPSLLAGKTGMIVSPTAFEKDAKGLATKPVGAGPFRLTEYVPNQSAKLVRFPEYWDKANIHLDAFELY
PAPEAATAVPALQSGRLDVAQIPGSQVEAAKAAGLEVQIIPSLVTTVLDVNITMKPFDNPKVVEAFKHALDRKALADTQT
FGLGVVNYQPFPPGYIGHEPSLENAFPYDPEKAKKLLAEAGFPDGVEVPLTTTGASSALAEQVQAQLAKVGVKITIETIP
AAQATQIMYIQHSRALATDGFAGRDSAVQAFQVLFGEQGLMNPGRQTPPELTAALQKVRETPLDDPSYPTVLRAATKIAV
EKMPNIFLFTTPRVLARKKNVSELGSYLAVQRFEGVRVG

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=516, Percent_Identity=29.4573643410853, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789887, Length=360, Percent_Identity=27.5, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789966, Length=343, Percent_Identity=26.8221574344023, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789397, Length=310, Percent_Identity=27.4193548387097, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787495, Length=391, Percent_Identity=26.5984654731458, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787762, Length=433, Percent_Identity=24.2494226327945, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54849; Mature: 54849

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQLRADSTLMGRRGFLGLGVLAGSSLLLAACGDDSGSVSSAGKEGGTLRWGWSAVTSW
CCCCCCCCCHHHCCCCCEEEHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCC
DPVTSSAGWDVHALSLAYAALTKLDEKGNPVPALAESWKYNADGTQVAFTLRAGLTFSDG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCEECCCCCEEEEEEEECEEECCC
TPLNATAVSKSLARGRDFAGSLVAAQLANVKTLAADDDARTVTIGLAATDYQIPSLLAGK
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCHHHCCC
TGMIVSPTAFEKDAKGLATKPVGAGPFRLTEYVPNQSAKLVRFPEYWDKANIHLDAFELY
CCCEECCCCCCCCCCCCCCCCCCCCCCCHHECCCCCCCCEEECCHHHCCCCEEEEEEEEC
PAPEAATAVPALQSGRLDVAQIPGSQVEAAKAAGLEVQIIPSLVTTVLDVNITMKPFDNP
CCCCHHHHCCHHHCCCEEHEECCCCHHHHHHHCCCEEEEHHHHHHHHHCCCEEECCCCCC
KVVEAFKHALDRKALADTQTFGLGVVNYQPFPPGYIGHEPSLENAFPYDPEKAKKLLAEA
HHHHHHHHHHHHHHHHCCHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GFPDGVEVPLTTTGASSALAEQVQAQLAKVGVKITIETIPAAQATQIMYIQHSRALATDG
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCCEECCC
FAGRDSAVQAFQVLFGEQGLMNPGRQTPPELTAALQKVRETPLDDPSYPTVLRAATKIAV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EKMPNIFLFTTPRVLARKKNVSELGSYLAVQRFEGVRVG
HHCCCEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAQLRADSTLMGRRGFLGLGVLAGSSLLLAACGDDSGSVSSAGKEGGTLRWGWSAVTSW
CCCCCCCCCHHHCCCCCEEEHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCC
DPVTSSAGWDVHALSLAYAALTKLDEKGNPVPALAESWKYNADGTQVAFTLRAGLTFSDG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCEECCCCCEEEEEEEECEEECCC
TPLNATAVSKSLARGRDFAGSLVAAQLANVKTLAADDDARTVTIGLAATDYQIPSLLAGK
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCHHHCCC
TGMIVSPTAFEKDAKGLATKPVGAGPFRLTEYVPNQSAKLVRFPEYWDKANIHLDAFELY
CCCEECCCCCCCCCCCCCCCCCCCCCCCHHECCCCCCCCEEECCHHHCCCCEEEEEEEEC
PAPEAATAVPALQSGRLDVAQIPGSQVEAAKAAGLEVQIIPSLVTTVLDVNITMKPFDNP
CCCCHHHHCCHHHCCCEEHEECCCCHHHHHHHCCCEEEEHHHHHHHHHCCCEEECCCCCC
KVVEAFKHALDRKALADTQTFGLGVVNYQPFPPGYIGHEPSLENAFPYDPEKAKKLLAEA
HHHHHHHHHHHHHHHHCCHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
GFPDGVEVPLTTTGASSALAEQVQAQLAKVGVKITIETIPAAQATQIMYIQHSRALATDG
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCCEECCC
FAGRDSAVQAFQVLFGEQGLMNPGRQTPPELTAALQKVRETPLDDPSYPTVLRAATKIAV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EKMPNIFLFTTPRVLARKKNVSELGSYLAVQRFEGVRVG
HHCCCEEEEECCHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA