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The map label for this gene is asnO [H]

Identifier: 158314126

GI number: 158314126

Start: 2741410

End: 2743191

Strand: Direct

Name: asnO [H]

Synonym: Franean1_2293

Alternate gene names: 158314126

Gene position: 2741410-2743191 (Clockwise)

Preceding gene: 158314125

Following gene: 158314127

Centisome position: 30.52

GC content: 72.5

Gene sequence:

>1782_bases
ATGTGCGGGCTGAGCGGGGAGCTGAGGTTCGACGGCCGTGAGGCGGACGTCGCCGCGGTGGGGCGGATGACCGCCGCAAT
GGAGGCGCGGGGCCCGGACGGGGTCGGCCTGTGGGCCACCGGCCCGGTGGCGTTCGGCCACCGTCGGCTGAAGATCATTG
ATCTGTCGGAGCGGGGCGCCCAGCCCATGGTCGACACCGCGCTCGGCCTCACCGCGGTGTTCAACGGTTGCATCTACAAC
TACCAGGAGCTGCGCGACCGGCTCGTCGACGCCGGCTACCGCTTCTTCTCCACCTCCGACACCGAGGTGATCCTCAAGGC
GTACCACCACTGGGGCCCCCGGTGCGTCGACCACTTCGCCGGCATGTTCGCCTTCGCCGTGTACGAGCGCGACACCGGCC
GGCTCGTCCTCGTCCGCGACCGGCTCGGGATCAAGCCGCTCTACGTGACGCGCGACCCGCACCGCCTGCGGTTCGCCTCG
ACCCTGCCGGCCCTGCTCGCCGGCGGCGGGGTGTCCTCCGACATCGACCTGGTCGCCTTCCACCACTACCTGTCGTTCCA
CTCGGTGGTCCCGCCGCCGCACACGATCCTGACCGGCGTGCGCAAGCTGCCGCAGGCCACCATCCGGACCGTCGAGGCGG
ACGGCACGACCCGCGACGAGGTCTACTGGCGGCCCGACTCCACCCGTCAGGAGGAGCACGCCTCCTGGACGGCGGACGAC
TGGCGGGACGCGATCGGCGAGCGGCTGCGCACCGCCGTGCGCCGGCGCATGGTCGCCGACGTCCCGGTCGGGGTGCTGCT
CTCCGGCGGCCTGGACTCCAGCCTCATCGTCGCGCTGCTCGCCGAGGCCGGCCAGCGTGACCTCGCCACGTTCAGCGTCG
GCTTCGAGGCCGCCGGCGGCGAGTCCGGCGACGAGTTCCACTACTCGGACATCATCGCCCGCCGCTTCGGCACCGACCAC
CACCAGATCCGCGTCCCCGGCGACCGGCTGCTGCCGGCGATCGACGCGGCCGTCGCCGCGATGAGCGAGCCGATGGTCAG
CCACGACTGCGTCGCGTTCTACCTGCTCTCGCAGGAGGTGGCCCGGCACGTCAAGGTCGTGCAGTCCGGCCAGGGCGCGG
ACGAGGTCTTCGCCGGCTACTCCTGGTACCCGCCGCTGGCGACCGTCCCGCGCGGCGACGCCGTCGACGCCTACCTGCGG
GTGTTCGCCGACCGGCCGCACGCCGAGCTGCCGGCGATGGTCGAGTCGCACCACCTCATCGAGGGTGACGCCAGTCGGCA
CTTCGTCGCCGAGCGGTTCGCCGAGCCCGGCGCCGAGACCTCGGTCGACGCCGCGCTGCGCATCGACTCCACGGTGATGC
TGGTCGACGACCCGGTGAAGCGGGTCGACAACATGACGATGGCGGCCGGCCTGGAGGCCCGCACGCCCTTCCTCGACCAC
GAACTCGTCGAGCTCGCCGCCGCGTGCCCGCCCGAGCTGAAGCTCGCCTCCGGCGGCAAGGGCGTGCTCAAGGACCTCAG
CCGGCCGCTGATGCCGGTGGACGTCATCGACCGGCCGAAGGGGTACTTCCCGGTGCCGGCGATCCGCCACCTCGGCGGCC
CGTTCCTCGCCCGGGTCGCCGACGCGCTGACCGACCCGGTCGCCCGGGGCCGCGGGCTGTTCCGCAAGGACTGGGTCGAC
GAGATGCTCGCCGACCCGAACTCGAACCGTACGACGCTCGGGGCGAACGCGCTCTGGCAGGCCGCCCTGCTCGAGATGTG
GCTGCAGACACAGGGGGTGTGA

Upstream 100 bases:

>100_bases
CGCCCCGTCGGTCCCGGCGGGTTCCCGGAACCGGGTGGGCGGACGTCGTCGAGGAGGGGAAGAACGGCCGTCTGACCAAC
CGATCGACGGGAGCTGGACC

Downstream 100 bases:

>100_bases
ATGATCCGTCCGGTCGAGTCGGCGCTCACCGGCGTCCCGTCCCGGACCGCCGCGGCGCGGTCTGAGACCACCGCGGCACC
GGCTGGCGCCCTGCCGGCCG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MCGLSGELRFDGREADVAAVGRMTAAMEARGPDGVGLWATGPVAFGHRRLKIIDLSERGAQPMVDTALGLTAVFNGCIYN
YQELRDRLVDAGYRFFSTSDTEVILKAYHHWGPRCVDHFAGMFAFAVYERDTGRLVLVRDRLGIKPLYVTRDPHRLRFAS
TLPALLAGGGVSSDIDLVAFHHYLSFHSVVPPPHTILTGVRKLPQATIRTVEADGTTRDEVYWRPDSTRQEEHASWTADD
WRDAIGERLRTAVRRRMVADVPVGVLLSGGLDSSLIVALLAEAGQRDLATFSVGFEAAGGESGDEFHYSDIIARRFGTDH
HQIRVPGDRLLPAIDAAVAAMSEPMVSHDCVAFYLLSQEVARHVKVVQSGQGADEVFAGYSWYPPLATVPRGDAVDAYLR
VFADRPHAELPAMVESHHLIEGDASRHFVAERFAEPGAETSVDAALRIDSTVMLVDDPVKRVDNMTMAAGLEARTPFLDH
ELVELAAACPPELKLASGGKGVLKDLSRPLMPVDVIDRPKGYFPVPAIRHLGGPFLARVADALTDPVARGRGLFRKDWVD
EMLADPNSNRTTLGANALWQAALLEMWLQTQGV

Sequences:

>Translated_593_residues
MCGLSGELRFDGREADVAAVGRMTAAMEARGPDGVGLWATGPVAFGHRRLKIIDLSERGAQPMVDTALGLTAVFNGCIYN
YQELRDRLVDAGYRFFSTSDTEVILKAYHHWGPRCVDHFAGMFAFAVYERDTGRLVLVRDRLGIKPLYVTRDPHRLRFAS
TLPALLAGGGVSSDIDLVAFHHYLSFHSVVPPPHTILTGVRKLPQATIRTVEADGTTRDEVYWRPDSTRQEEHASWTADD
WRDAIGERLRTAVRRRMVADVPVGVLLSGGLDSSLIVALLAEAGQRDLATFSVGFEAAGGESGDEFHYSDIIARRFGTDH
HQIRVPGDRLLPAIDAAVAAMSEPMVSHDCVAFYLLSQEVARHVKVVQSGQGADEVFAGYSWYPPLATVPRGDAVDAYLR
VFADRPHAELPAMVESHHLIEGDASRHFVAERFAEPGAETSVDAALRIDSTVMLVDDPVKRVDNMTMAAGLEARTPFLDH
ELVELAAACPPELKLASGGKGVLKDLSRPLMPVDVIDRPKGYFPVPAIRHLGGPFLARVADALTDPVARGRGLFRKDWVD
EMLADPNSNRTTLGANALWQAALLEMWLQTQGV
>Mature_593_residues
MCGLSGELRFDGREADVAAVGRMTAAMEARGPDGVGLWATGPVAFGHRRLKIIDLSERGAQPMVDTALGLTAVFNGCIYN
YQELRDRLVDAGYRFFSTSDTEVILKAYHHWGPRCVDHFAGMFAFAVYERDTGRLVLVRDRLGIKPLYVTRDPHRLRFAS
TLPALLAGGGVSSDIDLVAFHHYLSFHSVVPPPHTILTGVRKLPQATIRTVEADGTTRDEVYWRPDSTRQEEHASWTADD
WRDAIGERLRTAVRRRMVADVPVGVLLSGGLDSSLIVALLAEAGQRDLATFSVGFEAAGGESGDEFHYSDIIARRFGTDH
HQIRVPGDRLLPAIDAAVAAMSEPMVSHDCVAFYLLSQEVARHVKVVQSGQGADEVFAGYSWYPPLATVPRGDAVDAYLR
VFADRPHAELPAMVESHHLIEGDASRHFVAERFAEPGAETSVDAALRIDSTVMLVDDPVKRVDNMTMAAGLEARTPFLDH
ELVELAAACPPELKLASGGKGVLKDLSRPLMPVDVIDRPKGYFPVPAIRHLGGPFLARVADALTDPVARGRGLFRKDWVD
EMLADPNSNRTTLGANALWQAALLEMWLQTQGV

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=410, Percent_Identity=30.9756097560976, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25147557, Length=570, Percent_Identity=26.3157894736842, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71993933, Length=565, Percent_Identity=26.1946902654867, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325403, Length=574, Percent_Identity=26.3066202090592, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321563, Length=573, Percent_Identity=26.3525305410122, Blast_Score=143, Evalue=9e-35,
Organism=Drosophila melanogaster, GI45553209, Length=493, Percent_Identity=27.3833671399594, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 64894; Mature: 64894

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLSGELRFDGREADVAAVGRMTAAMEARGPDGVGLWATGPVAFGHRRLKIIDLSERGA
CCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCCEEEEEECCCCCC
QPMVDTALGLTAVFNGCIYNYQELRDRLVDAGYRFFSTSDTEVILKAYHHWGPRCVDHFA
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCHHHHHHHH
GMFAFAVYERDTGRLVLVRDRLGIKPLYVTRDPHRLRFASTLPALLAGGGVSSDIDLVAF
HHHHHHEEECCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
HHYLSFHSVVPPPHTILTGVRKLPQATIRTVEADGTTRDEVYWRPDSTRQEEHASWTADD
HHHHHHHCCCCCCHHHHHHHHHCCHHHHEEEECCCCCCCCEEECCCCCCCHHHCCCCHHH
WRDAIGERLRTAVRRRMVADVPVGVLLSGGLDSSLIVALLAEAGQRDLATFSVGFEAAGG
HHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHCCCCCCEEEEECEECCCC
ESGDEFHYSDIIARRFGTDHHQIRVPGDRLLPAIDAAVAAMSEPMVSHDCVAFYLLSQEV
CCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ARHVKVVQSGQGADEVFAGYSWYPPLATVPRGDAVDAYLRVFADRPHAELPAMVESHHLI
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCEE
EGDASRHFVAERFAEPGAETSVDAALRIDSTVMLVDDPVKRVDNMTMAAGLEARTPFLDH
CCCCHHHHHHHHHHCCCCCCCCCCEEEECCEEEEECCHHHHHCCCHHHCCCCCCCCCHHH
ELVELAAACPPELKLASGGKGVLKDLSRPLMPVDVIDRPKGYFPVPAIRHLGGPFLARVA
HHHHHHHCCCCCCEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
DALTDPVARGRGLFRKDWVDEMLADPNSNRTTLGANALWQAALLEMWLQTQGV
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGLSGELRFDGREADVAAVGRMTAAMEARGPDGVGLWATGPVAFGHRRLKIIDLSERGA
CCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCCEEEEEECCCCCC
QPMVDTALGLTAVFNGCIYNYQELRDRLVDAGYRFFSTSDTEVILKAYHHWGPRCVDHFA
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCHHHHHHHH
GMFAFAVYERDTGRLVLVRDRLGIKPLYVTRDPHRLRFASTLPALLAGGGVSSDIDLVAF
HHHHHHEEECCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
HHYLSFHSVVPPPHTILTGVRKLPQATIRTVEADGTTRDEVYWRPDSTRQEEHASWTADD
HHHHHHHCCCCCCHHHHHHHHHCCHHHHEEEECCCCCCCCEEECCCCCCCHHHCCCCHHH
WRDAIGERLRTAVRRRMVADVPVGVLLSGGLDSSLIVALLAEAGQRDLATFSVGFEAAGG
HHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHCCCCCCEEEEECEECCCC
ESGDEFHYSDIIARRFGTDHHQIRVPGDRLLPAIDAAVAAMSEPMVSHDCVAFYLLSQEV
CCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ARHVKVVQSGQGADEVFAGYSWYPPLATVPRGDAVDAYLRVFADRPHAELPAMVESHHLI
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCEE
EGDASRHFVAERFAEPGAETSVDAALRIDSTVMLVDDPVKRVDNMTMAAGLEARTPFLDH
CCCCHHHHHHHHHHCCCCCCCCCCEEEECCEEEEECCHHHHHCCCHHHCCCCCCCCCHHH
ELVELAAACPPELKLASGGKGVLKDLSRPLMPVDVIDRPKGYFPVPAIRHLGGPFLARVA
HHHHHHHCCCCCCEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
DALTDPVARGRGLFRKDWVDEMLADPNSNRTTLGANALWQAALLEMWLQTQGV
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]