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The map label for this gene is metK2 [H]

Identifier: 158313552

GI number: 158313552

Start: 2050678

End: 2051874

Strand: Direct

Name: metK2 [H]

Synonym: Franean1_1716

Alternate gene names: 158313552

Gene position: 2050678-2051874 (Clockwise)

Preceding gene: 158313551

Following gene: 158313553

Centisome position: 22.83

GC content: 70.26

Gene sequence:

>1197_bases
GTGGCGACGCGCCTTTTCACGTCCGAGTCCGTCACGGAAGGACACCCGGACAAGATCGCTGACCAGGTCAGCGACTCCAT
TCTCGATGCCATGCTCAAGGACGACCCGAAGAGCCGGGTCGCCGTCGAGACCATGATCACCACCGGTCAGGTGCACGTGG
CCGGCGAGGTGACCACCAAGACCTACGTCGACATCGCCTCGGTGGTCCGCGAGCGGATCCTGGAGATCGGTTACGACTCC
TCCAAGAAGGGCTTCGACGGCGCCTCCTGCGGCGTGAGCGTCTCGATCGGCTCGCAGTCGCCCGACATCGCCCAGGGTGT
CGACACCGCCCACGAGGCGCGCGTCGAGGGCTCCACCGAGGACGACCTCGACCGTCAGGGCGCCGGCGACCAGGGCCTGA
TGTTCGGGTTCGCCTGCGACGAGACCCCCGAGCTCATGCCGCTGCCGATCGCCCTGGCACACCGGCTCGCCCGGCGGCTG
TCCGCCGTCCGCAAGGACGGCCAGGTCGGCTACCTGCGCCCCGACGGCAAGACGCAGGTCACCATCGAGTACGAGGACGG
CAAGCCGGTCCGCCTGGACACCGTTGTGGTCTCCTCGCAGCACGCGGCCGACATCGACCTCGACACCCTGCTCGCGCCCG
ACGTCGCCGAGTACGTCGTGGAGCCGGAGCTCGCCCTCCTCGAGATCAGCACCGAGGGGCGCCGCCTGCTGGTGAACCCG
ACCGGTCGCTTCGAGATCGGTGGCCCGATGGGCGACGCCGGCCTCACCGGGCGCAAGATCATCGTGGACACCTACGGCGG
GTACGCCCGGCACGGCGGCGGCGCCTTCTCGGGTAAGGACCCGTCCAAGGTCGACCGCTCGGCTGCCTACGCCATGCGCT
GGGTCGCCAAGAACGTCGTGGCCGCGGGCCTGGCCAGCCGCTGTGAGGTCCAGGTCGCCTACGCGATCGGCAAGGCCCAC
CCGGTCGGCCTGTTCGTCGAGACCTTCGGCACGGGCAAGGTGCCGGACGCGCAGATCCAGGACGCCGTCACGCAGGTCTT
CGACCTCCGCCCGGCCGCGATCGTGCGGGACCTCGACCTGCTGCGCCCGATCTACGCGCAGACGGCCGCCTACGGGCACT
TCGGCCGCCCGGAGCTGGACTTCACCTGGGAGGCGACCTCCCGCGCCGACGCCCTAACAGCCGCCGTCAAGGGCTGA

Upstream 100 bases:

>100_bases
GACGCGCTGCACGACCGGAAGCCGGCCGGTGCGCGTACCCCTGAGTGAACCTCGGTAAACTCCGTCGGTATCAGTCCTGA
TCTCCCCAGGGGGTAGTCCA

Downstream 100 bases:

>100_bases
CGCCACCTGATCCGCCCGCAGGGGCGGAGCCGACCCCCATCGGGGACGGCTCCGCCCCTGCGCATTTTTGGACGGTTTGT
TTGCGGTGATCTGCTTTCCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase 2; MAT 2; Methionine adenosyltransferase 2 [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MATRLFTSESVTEGHPDKIADQVSDSILDAMLKDDPKSRVAVETMITTGQVHVAGEVTTKTYVDIASVVRERILEIGYDS
SKKGFDGASCGVSVSIGSQSPDIAQGVDTAHEARVEGSTEDDLDRQGAGDQGLMFGFACDETPELMPLPIALAHRLARRL
SAVRKDGQVGYLRPDGKTQVTIEYEDGKPVRLDTVVVSSQHAADIDLDTLLAPDVAEYVVEPELALLEISTEGRRLLVNP
TGRFEIGGPMGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLASRCEVQVAYAIGKAH
PVGLFVETFGTGKVPDAQIQDAVTQVFDLRPAAIVRDLDLLRPIYAQTAAYGHFGRPELDFTWEATSRADALTAAVKG

Sequences:

>Translated_398_residues
MATRLFTSESVTEGHPDKIADQVSDSILDAMLKDDPKSRVAVETMITTGQVHVAGEVTTKTYVDIASVVRERILEIGYDS
SKKGFDGASCGVSVSIGSQSPDIAQGVDTAHEARVEGSTEDDLDRQGAGDQGLMFGFACDETPELMPLPIALAHRLARRL
SAVRKDGQVGYLRPDGKTQVTIEYEDGKPVRLDTVVVSSQHAADIDLDTLLAPDVAEYVVEPELALLEISTEGRRLLVNP
TGRFEIGGPMGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLASRCEVQVAYAIGKAH
PVGLFVETFGTGKVPDAQIQDAVTQVFDLRPAAIVRDLDLLRPIYAQTAAYGHFGRPELDFTWEATSRADALTAAVKG
>Mature_397_residues
ATRLFTSESVTEGHPDKIADQVSDSILDAMLKDDPKSRVAVETMITTGQVHVAGEVTTKTYVDIASVVRERILEIGYDSS
KKGFDGASCGVSVSIGSQSPDIAQGVDTAHEARVEGSTEDDLDRQGAGDQGLMFGFACDETPELMPLPIALAHRLARRLS
AVRKDGQVGYLRPDGKTQVTIEYEDGKPVRLDTVVVSSQHAADIDLDTLLAPDVAEYVVEPELALLEISTEGRRLLVNPT
GRFEIGGPMGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLASRCEVQVAYAIGKAHP
VGLFVETFGTGKVPDAQIQDAVTQVFDLRPAAIVRDLDLLRPIYAQTAAYGHFGRPELDFTWEATSRADALTAAVKG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=393, Percent_Identity=53.4351145038168, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI4557737, Length=387, Percent_Identity=54.7803617571059, Blast_Score=372, Evalue=1e-103,
Organism=Escherichia coli, GI1789311, Length=392, Percent_Identity=60.2040816326531, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17538494, Length=384, Percent_Identity=53.6458333333333, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17538498, Length=384, Percent_Identity=53.6458333333333, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25145633, Length=388, Percent_Identity=52.319587628866, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17551082, Length=404, Percent_Identity=50.4950495049505, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565905, Length=386, Percent_Identity=48.1865284974093, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=52.3668639053254, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=50.7288629737609, Blast_Score=307, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI32565903, Length=314, Percent_Identity=51.9108280254777, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=54.2168674698795, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323209, Length=389, Percent_Identity=56.0411311053985, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=55.2162849872774, Blast_Score=394, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45552159, Length=396, Percent_Identity=51.010101010101, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=396, Percent_Identity=51.010101010101, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=396, Percent_Identity=51.010101010101, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=396, Percent_Identity=51.010101010101, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=396, Percent_Identity=51.010101010101, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=396, Percent_Identity=51.010101010101, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=396, Percent_Identity=51.010101010101, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580525, Length=396, Percent_Identity=51.010101010101, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580541, Length=381, Percent_Identity=49.6062992125984, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24580539, Length=130, Percent_Identity=53.0769230769231, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42491; Mature: 42360

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRLFTSESVTEGHPDKIADQVSDSILDAMLKDDPKSRVAVETMITTGQVHVAGEVTTK
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEECCEEEEEECCCHH
TYVDIASVVRERILEIGYDSSKKGFDGASCGVSVSIGSQSPDIAQGVDTAHEARVEGSTE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHCCCCCC
DDLDRQGAGDQGLMFGFACDETPELMPLPIALAHRLARRLSAVRKDGQVGYLRPDGKTQV
HHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEE
TIEYEDGKPVRLDTVVVSSQHAADIDLDTLLAPDVAEYVVEPELALLEISTEGRRLLVNP
EEEECCCCCEEEEEEEEECCCCCCCCHHHHCCCHHHHHHCCCCEEEEEECCCCCEEEECC
TGRFEIGGPMGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVV
CCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAGLASRCEVQVAYAIGKAHPVGLFVETFGTGKVPDAQIQDAVTQVFDLRPAAIVRDLDL
HHHHHHHCEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LRPIYAQTAAYGHFGRPELDFTWEATSRADALTAAVKG
HHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHCCCC
>Mature Secondary Structure 
ATRLFTSESVTEGHPDKIADQVSDSILDAMLKDDPKSRVAVETMITTGQVHVAGEVTTK
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEECCEEEEEECCCHH
TYVDIASVVRERILEIGYDSSKKGFDGASCGVSVSIGSQSPDIAQGVDTAHEARVEGSTE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHCCCCCC
DDLDRQGAGDQGLMFGFACDETPELMPLPIALAHRLARRLSAVRKDGQVGYLRPDGKTQV
HHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEE
TIEYEDGKPVRLDTVVVSSQHAADIDLDTLLAPDVAEYVVEPELALLEISTEGRRLLVNP
EEEECCCCCEEEEEEEEECCCCCCCCHHHHCCCHHHHHHCCCCEEEEEECCCCCEEEECC
TGRFEIGGPMGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVV
CCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAGLASRCEVQVAYAIGKAHPVGLFVETFGTGKVPDAQIQDAVTQVFDLRPAAIVRDLDL
HHHHHHHCEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LRPIYAQTAAYGHFGRPELDFTWEATSRADALTAAVKG
HHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA