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The map label for this gene is priA [H]

Identifier: 158313553

GI number: 158313553

Start: 2052280

End: 2054661

Strand: Direct

Name: priA [H]

Synonym: Franean1_1717

Alternate gene names: 158313553

Gene position: 2052280-2054661 (Clockwise)

Preceding gene: 158313552

Following gene: 158313554

Centisome position: 22.85

GC content: 79.68

Gene sequence:

>2382_bases
GTGGTTCCGGCTGACGAGCTGCCGGTGGCCCGGGTCGTGGTCGACACCCCGCTCGTCCACCTTGACCGCCCGTTCGACTA
CCTCGTGCCGGCCGCCATGGCCGCCGCCGCGGTTCCCGGCTCACGGGTGCGCGTCCGCTTCGGTGGGCGCCTGGTGGACG
GGTTCGTCCTCGAGAGGTCCGAGCGGTCCGATCACACCGGGCGTCTCACGCCGCTGGCCAGGTCGGTGTCGGCGGAGCCG
GTGCTCTCCGAGCCGGTCGCCCGCCTCGCCCGCGCCGTCGCCGACAGATACGCCGGGACGATGGCCGACGTGCTGCGCCT
GGCCGTCCCGCCGCGCCATGCCCGCGCCGAGGCTGAGCCGGTTGTCACCGGTCCCGGCGTCGGCGTGGCGCCGGCGGCTA
CCGCGAGCGGTGGCGCGGCCGAGCTGGGCGGCGGCCGGTCCGGTGATCCTGGCCGCGCGGGGTGGTCGATCTATCCCGAC
GGCCCCGACTTCCTGGCGGCGCTGTCGAGCGGGCGCTCGCCGCGGGCGGTCTGGTGGGCGCCGCCGGGGCCCTCCTGGCC
GGCGATGATCGCCGTGGCGGTCGCGGCCACGGTGGCCTCCGGGCGCGGCGCGCTCGTCGTCGTTCCCGACCACCGGGACG
CTGACCGGGTCGCCGCGGCGCTGACACCTCCCGAGACCGAGGCCGAGGCCGAGACCAAGGCCAAGGCCGGGCCGGCGGCG
GGTGGCGTCCCGGCCGTGCTGCGGGCCGATCTCGGCCCGGCGGAGCGGTACCGGCGCTTCCTGGCGGTCCGCCGCGGGCA
GACGCGGGTGGTCGTGGGGACCCGTGCCGCGGTGTTCGCGCCCGTGGCCGACCTCGGCCTGCTCGTCGTCTGGGACGACG
GGGACGACCTGCACTCCGAACCTCGGGCGCCCTACCCGCACACGCGCGACGTGGCGGTCCTGCGGGCCCGGGAGGAGGGC
TGCGCGGTGCTGGTCGGCGGGTTCTCGCCGTCGGTGGACGCCGAGGCCCTGGCCCGGGTGGGCTGGGCCGGCCGGCTGGC
GGCCGGCCGGGACGCCGTGCGGCGGCTCGCGCCCCGGGTGGAGGCGGTCGGCTCCGACTTCGAGGCGTCCATCGACCCGG
CCGCGCGGGCGGCCCGGCTGCCCTCGCTCGCCGTGCGTGCCGCGCGCGCCGCGCTGGCCGGTGGCGCGCCGGTGCTGGTC
CAGGTGCCGCGGCGGGGATATCACCCGTCCATCGCCTGCGCGGACTGCCGCCGCCCGGCCCGGTGCCGGAGCTGCTCCGG
CCCGCTCGGCCGGTCCGCCGCCGGGGCCGCGCTGGCCTGCCGCTGGTGCGGGCGCCCGGTGCCGCGGCCCGCCGGCCATC
CGCCGGCGCCTGGGCAGGAGCCCGTCCGGGGGCGAGCCGGGGCTTTCGGCGCTGCTGTGGGCTCCGGTCGTTCCGCGGTG
TCCGGGGGCGGCGTGGGTGGGTGGCGGTGCACGGGTTGCGGCGGAACGCGCCTGCGCGCGTCGGTGACCGGTGACCGCCG
CACCGCCGAGGAACTGGGGCGGGCGTTTCCCGGCATCCCGGTGCGGACATCCGGGCGCGACACGGTGCTGGCCACGGTGC
CGGCCGAGCCGGCGCTCGTGATCGCGACACCGGGCGCGGAGCCGGTCGCCGAGGCAGGCTACGGGGCGGTGCTGCTCCTC
GACGGCTGGGCGCTGCTCGGGCGTCCCGATCTGCGGGCCGGCGAGGAGACGCTGCGCCGGTGGATGGCCGCGTCCGCGCT
GGCCCGGCCGGCCCCGGCCGGTGGGCTGGTGGTCGTGGCGGCGGATCTCGGGCTCGGGCCCGTGCAGGCGCTGGTGCGCT
GGGATCCCGGGCTGTTCGTCGCCCGGGAGTCGGACGAGCGCGCCGAGCTCGGCTTCCCCCCGGTGAGCCGGGTGGCGGCG
GTGGATGGCACCCCCGCGGCGCTCGACGACCTGCTGGCGGCCGCCACGCTGCCGGCCGGCGCGGAGGTCCTGGGCCCGGT
GCCGGTCCCGAGACGGGAGCCGGTCCCGAGACGGGAGCCGGTCCCGAGGCAGGAACCGGGCCGCGGGGCCGGCGGGGAGC
CGGCTCCCGGGCCGGGCCACGCACCGGGCCGTGGGTCGGGCCGCGCGCAGGGGCGTGAGCAGAGCAGGGGGCTGGGTCGG
GTGCGGGCGGGGTCCGGAGATCCGAACGAGCCGGGGCGCCGGGGGCCGCCTGAGCAGGTGAAGCCACCGGAGCCGGCGGA
GGAGCCCGAGCGTCTGATCATCCGGGTGCGCCGGGAGCAGGGCGCGGACCTCGCCGCCGCGCTGCGGGCCGCCCGCGGGG
TGCTCGACGCCCGCCGTGCGGGTCGGGTGCGCGTCCAGCTCGACCCGCTGACGATCGGCTGA

Upstream 100 bases:

>100_bases
CCCGGCGCGGCCCGCCGCCGGCGCGGCGAAGTCTGGCGCCGCCCCGGCGAAGCGAGGTGCCGTCGCCGCGAAGCCGGGCG
GCTCCGAGAAGCCCGGCGCG

Downstream 100 bases:

>100_bases
CGGCCGTTGGCCGCCGGCCGGCGGACCGGGGCGGGGCCGCCGGTCCAATCCGGCGCCGGTTCCCGCGCGACCCGTAGCAT
GAAAGCGTCCGATCTCGCCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MVPADELPVARVVVDTPLVHLDRPFDYLVPAAMAAAAVPGSRVRVRFGGRLVDGFVLERSERSDHTGRLTPLARSVSAEP
VLSEPVARLARAVADRYAGTMADVLRLAVPPRHARAEAEPVVTGPGVGVAPAATASGGAAELGGGRSGDPGRAGWSIYPD
GPDFLAALSSGRSPRAVWWAPPGPSWPAMIAVAVAATVASGRGALVVVPDHRDADRVAAALTPPETEAEAETKAKAGPAA
GGVPAVLRADLGPAERYRRFLAVRRGQTRVVVGTRAAVFAPVADLGLLVVWDDGDDLHSEPRAPYPHTRDVAVLRAREEG
CAVLVGGFSPSVDAEALARVGWAGRLAAGRDAVRRLAPRVEAVGSDFEASIDPAARAARLPSLAVRAARAALAGGAPVLV
QVPRRGYHPSIACADCRRPARCRSCSGPLGRSAAGAALACRWCGRPVPRPAGHPPAPGQEPVRGRAGAFGAAVGSGRSAV
SGGGVGGWRCTGCGGTRLRASVTGDRRTAEELGRAFPGIPVRTSGRDTVLATVPAEPALVIATPGAEPVAEAGYGAVLLL
DGWALLGRPDLRAGEETLRRWMAASALARPAPAGGLVVVAADLGLGPVQALVRWDPGLFVARESDERAELGFPPVSRVAA
VDGTPAALDDLLAAATLPAGAEVLGPVPVPRREPVPRREPVPRQEPGRGAGGEPAPGPGHAPGRGSGRAQGREQSRGLGR
VRAGSGDPNEPGRRGPPEQVKPPEPAEEPERLIIRVRREQGADLAAALRAARGVLDARRAGRVRVQLDPLTIG

Sequences:

>Translated_793_residues
MVPADELPVARVVVDTPLVHLDRPFDYLVPAAMAAAAVPGSRVRVRFGGRLVDGFVLERSERSDHTGRLTPLARSVSAEP
VLSEPVARLARAVADRYAGTMADVLRLAVPPRHARAEAEPVVTGPGVGVAPAATASGGAAELGGGRSGDPGRAGWSIYPD
GPDFLAALSSGRSPRAVWWAPPGPSWPAMIAVAVAATVASGRGALVVVPDHRDADRVAAALTPPETEAEAETKAKAGPAA
GGVPAVLRADLGPAERYRRFLAVRRGQTRVVVGTRAAVFAPVADLGLLVVWDDGDDLHSEPRAPYPHTRDVAVLRAREEG
CAVLVGGFSPSVDAEALARVGWAGRLAAGRDAVRRLAPRVEAVGSDFEASIDPAARAARLPSLAVRAARAALAGGAPVLV
QVPRRGYHPSIACADCRRPARCRSCSGPLGRSAAGAALACRWCGRPVPRPAGHPPAPGQEPVRGRAGAFGAAVGSGRSAV
SGGGVGGWRCTGCGGTRLRASVTGDRRTAEELGRAFPGIPVRTSGRDTVLATVPAEPALVIATPGAEPVAEAGYGAVLLL
DGWALLGRPDLRAGEETLRRWMAASALARPAPAGGLVVVAADLGLGPVQALVRWDPGLFVARESDERAELGFPPVSRVAA
VDGTPAALDDLLAAATLPAGAEVLGPVPVPRREPVPRREPVPRQEPGRGAGGEPAPGPGHAPGRGSGRAQGREQSRGLGR
VRAGSGDPNEPGRRGPPEQVKPPEPAEEPERLIIRVRREQGADLAAALRAARGVLDARRAGRVRVQLDPLTIG
>Mature_793_residues
MVPADELPVARVVVDTPLVHLDRPFDYLVPAAMAAAAVPGSRVRVRFGGRLVDGFVLERSERSDHTGRLTPLARSVSAEP
VLSEPVARLARAVADRYAGTMADVLRLAVPPRHARAEAEPVVTGPGVGVAPAATASGGAAELGGGRSGDPGRAGWSIYPD
GPDFLAALSSGRSPRAVWWAPPGPSWPAMIAVAVAATVASGRGALVVVPDHRDADRVAAALTPPETEAEAETKAKAGPAA
GGVPAVLRADLGPAERYRRFLAVRRGQTRVVVGTRAAVFAPVADLGLLVVWDDGDDLHSEPRAPYPHTRDVAVLRAREEG
CAVLVGGFSPSVDAEALARVGWAGRLAAGRDAVRRLAPRVEAVGSDFEASIDPAARAARLPSLAVRAARAALAGGAPVLV
QVPRRGYHPSIACADCRRPARCRSCSGPLGRSAAGAALACRWCGRPVPRPAGHPPAPGQEPVRGRAGAFGAAVGSGRSAV
SGGGVGGWRCTGCGGTRLRASVTGDRRTAEELGRAFPGIPVRTSGRDTVLATVPAEPALVIATPGAEPVAEAGYGAVLLL
DGWALLGRPDLRAGEETLRRWMAASALARPAPAGGLVVVAADLGLGPVQALVRWDPGLFVARESDERAELGFPPVSRVAA
VDGTPAALDDLLAAATLPAGAEVLGPVPVPRREPVPRREPVPRQEPGRGAGGEPAPGPGHAPGRGSGRAQGREQSRGLGR
VRAGSGDPNEPGRRGPPEQVKPPEPAEEPERLIIRVRREQGADLAAALRAARGVLDARRAGRVRVQLDPLTIG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=338, Percent_Identity=25.7396449704142, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 81480; Mature: 81480

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPADELPVARVVVDTPLVHLDRPFDYLVPAAMAAAAVPGSRVRVRFGGRLVDGFVLERS
CCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCEEEEHHEEECC
ERSDHTGRLTPLARSVSAEPVLSEPVARLARAVADRYAGTMADVLRLAVPPRHARAEAEP
CCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
VVTGPGVGVAPAATASGGAAELGGGRSGDPGRAGWSIYPDGPDFLAALSSGRSPRAVWWA
EEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCEEEEC
PPGPSWPAMIAVAVAATVASGRGALVVVPDHRDADRVAAALTPPETEAEAETKAKAGPAA
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHEEEECCCCCCCCHHHHHHCCCCCC
GGVPAVLRADLGPAERYRRFLAVRRGQTRVVVGTRAAVFAPVADLGLLVVWDDGDDLHSE
CCCCHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCEEEEECCCCCCCCC
PRAPYPHTRDVAVLRAREEGCAVLVGGFSPSVDAEALARVGWAGRLAAGRDAVRRLAPRV
CCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
EAVGSDFEASIDPAARAARLPSLAVRAARAALAGGAPVLVQVPRRGYHPSIACADCRRPA
HHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEHHCCCCH
RCRSCSGPLGRSAAGAALACRWCGRPVPRPAGHPPAPGQEPVRGRAGAFGAAVGSGRSAV
HHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
SGGGVGGWRCTGCGGTRLRASVTGDRRTAEELGRAFPGIPVRTSGRDTVLATVPAEPALV
CCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCEECCCCCEEEEECCCCCEEE
IATPGAEPVAEAGYGAVLLLDGWALLGRPDLRAGEETLRRWMAASALARPAPAGGLVVVA
EECCCCCHHHHCCCCEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
ADLGLGPVQALVRWDPGLFVARESDERAELGFPPVSRVAAVDGTPAALDDLLAAATLPAG
ECCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHCCCCC
AEVLGPVPVPRREPVPRREPVPRQEPGRGAGGEPAPGPGHAPGRGSGRAQGREQSRGLGR
CHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCE
VRAGSGDPNEPGRRGPPEQVKPPEPAEEPERLIIRVRREQGADLAAALRAARGVLDARRA
EECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEHHHCCCHHHHHHHHHHHHHHHHHC
GRVRVQLDPLTIG
CEEEEEECCEECC
>Mature Secondary Structure
MVPADELPVARVVVDTPLVHLDRPFDYLVPAAMAAAAVPGSRVRVRFGGRLVDGFVLERS
CCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCEEEEHHEEECC
ERSDHTGRLTPLARSVSAEPVLSEPVARLARAVADRYAGTMADVLRLAVPPRHARAEAEP
CCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
VVTGPGVGVAPAATASGGAAELGGGRSGDPGRAGWSIYPDGPDFLAALSSGRSPRAVWWA
EEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCEEEEC
PPGPSWPAMIAVAVAATVASGRGALVVVPDHRDADRVAAALTPPETEAEAETKAKAGPAA
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHEEEECCCCCCCCHHHHHHCCCCCC
GGVPAVLRADLGPAERYRRFLAVRRGQTRVVVGTRAAVFAPVADLGLLVVWDDGDDLHSE
CCCCHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCEEEEECCCCCCCCC
PRAPYPHTRDVAVLRAREEGCAVLVGGFSPSVDAEALARVGWAGRLAAGRDAVRRLAPRV
CCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
EAVGSDFEASIDPAARAARLPSLAVRAARAALAGGAPVLVQVPRRGYHPSIACADCRRPA
HHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEHHCCCCH
RCRSCSGPLGRSAAGAALACRWCGRPVPRPAGHPPAPGQEPVRGRAGAFGAAVGSGRSAV
HHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
SGGGVGGWRCTGCGGTRLRASVTGDRRTAEELGRAFPGIPVRTSGRDTVLATVPAEPALV
CCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCEECCCCCEEEEECCCCCEEE
IATPGAEPVAEAGYGAVLLLDGWALLGRPDLRAGEETLRRWMAASALARPAPAGGLVVVA
EECCCCCHHHHCCCCEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
ADLGLGPVQALVRWDPGLFVARESDERAELGFPPVSRVAAVDGTPAALDDLLAAATLPAG
ECCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHCCCCC
AEVLGPVPVPRREPVPRREPVPRQEPGRGAGGEPAPGPGHAPGRGSGRAQGREQSRGLGR
CHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCE
VRAGSGDPNEPGRRGPPEQVKPPEPAEEPERLIIRVRREQGADLAAALRAARGVLDARRA
EECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEHHHCCCHHHHHHHHHHHHHHHHHC
GRVRVQLDPLTIG
CEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]