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The map label for this gene is carA [H]

Identifier: 158313545

GI number: 158313545

Start: 2041745

End: 2042938

Strand: Direct

Name: carA [H]

Synonym: Franean1_1709

Alternate gene names: 158313545

Gene position: 2041745-2042938 (Clockwise)

Preceding gene: 158313544

Following gene: 158313546

Centisome position: 22.73

GC content: 72.61

Gene sequence:

>1194_bases
GTGACCGGCTTGGACAGGCAGCCGGCTCCGCCGGAGCGGGAACAGGCCCGGCGCGGGCCGGGAGCGCCCCGCCGCGCGGT
GCTCATGCTCGAGGACGGCCGGAGCTTCGCCGGGGACGCGTTCGGCTCGGTCGGCGAGGCGTTCGGCGAGGCGGTCTTCT
CCACCGGGATGACCGGCTACCAGGAGACCCTCACCGACCCGTCGTTCCACCGTCAGGTCGTGATCATGACGGCGCCGCAC
ATCGGCAACACCGGGGTGAACGACACCGACTACGAGTCCGACCGCATCCAGGTGGCCGGCTTCGTTGTGCGGGACCCGAG
CCGGCTGGCGTCGAACTGGCGCGCCCAACGCACCCTGGACGACGAGCTCGAGAACGCCGGCGTGGTCGGGATCAGCGGGG
TCGACACCCGCGCGCTGACCCGTCACCTGCGCGAGCGCGGCGCGATGCGGTGCGGGGTCAGCAGCACCGACACCGATCTC
GACTCGCTGCTCGACCGGGTGCGCGAGTCGCCGGAGATGGTCGGCGCGGACCTCGCCCCGGAGGTCAGCACGGACAAGCC
CTACGTCGTCGAGGCGCGGTCGGGCCTGCCGCTCTTCACCGTCGCCGCGCTGGACCTGGGCATCAAGCGGAACACCCCGC
TCTCCATGGCAGCGCTGGGCTGCGAGGTGCACGTGCTGCCGGCCCGCAGCACGGCCGCCGAGCTGCTGGCCCTCTCGCCC
GACGGGGTCTTCCTCTCGAACGGCCCGGGTGACCCGGCCCGCGCGGACTACGCGGTCGAGACGCTCACCGGGGTGCTGGA
GGCGGGTGTCCCCGTCTTCGGGATCTGTTTCGGCAACCAGGTGCTCGCACGGGCCCTGGGCTTCGAGACGTACAAGCTGA
CCTACGGCCACCGCGGCGTGAACCAGCCCGTGGCCGACACCCGGACCGGCCGGATCGCGGTCACCAGCCACAACCACGGC
TTCGCGGTGCGCGCGCCGCTGACCGGTACGACCGACACCCCCTACGGGCGGGTCGAGGTGAGCCACGTGGCGCTCAACGA
CGACGTGGTGGAGGGCCTGACCTGCCTGGACGTGCCGGCGTTCAGTGTCCAGTTCCATCCCGAGGCGGCGCCTGGCCCGC
ACGACGCCCAGGGACTGTTCGACCGGTTCTGCGGCCTGATGGCGGCCGGCCGGCGGAAGCGGGGAGAAGGCTGA

Upstream 100 bases:

>100_bases
CGGGAGGAGTACCCGTGACAGCGGCGCCCTGGCGGTCTGCCAGGGGTGGAGGGGGCCTGGCCGGCAGCCGGGCACGACGG
ATCGATGCGGGGGTGAGCCG

Downstream 100 bases:

>100_bases
TGCCCAGGCGCGAGGACCTGCGCTCCGTGCTGGTGATCGGCTCCGGGCCGATCGTCATCGGCCAGGCGTGCGAGTTCGAC
TATTCCGGGACGCAGGCGTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTGLDRQPAPPEREQARRGPGAPRRAVLMLEDGRSFAGDAFGSVGEAFGEAVFSTGMTGYQETLTDPSFHRQVVIMTAPH
IGNTGVNDTDYESDRIQVAGFVVRDPSRLASNWRAQRTLDDELENAGVVGISGVDTRALTRHLRERGAMRCGVSSTDTDL
DSLLDRVRESPEMVGADLAPEVSTDKPYVVEARSGLPLFTVAALDLGIKRNTPLSMAALGCEVHVLPARSTAAELLALSP
DGVFLSNGPGDPARADYAVETLTGVLEAGVPVFGICFGNQVLARALGFETYKLTYGHRGVNQPVADTRTGRIAVTSHNHG
FAVRAPLTGTTDTPYGRVEVSHVALNDDVVEGLTCLDVPAFSVQFHPEAAPGPHDAQGLFDRFCGLMAAGRRKRGEG

Sequences:

>Translated_397_residues
MTGLDRQPAPPEREQARRGPGAPRRAVLMLEDGRSFAGDAFGSVGEAFGEAVFSTGMTGYQETLTDPSFHRQVVIMTAPH
IGNTGVNDTDYESDRIQVAGFVVRDPSRLASNWRAQRTLDDELENAGVVGISGVDTRALTRHLRERGAMRCGVSSTDTDL
DSLLDRVRESPEMVGADLAPEVSTDKPYVVEARSGLPLFTVAALDLGIKRNTPLSMAALGCEVHVLPARSTAAELLALSP
DGVFLSNGPGDPARADYAVETLTGVLEAGVPVFGICFGNQVLARALGFETYKLTYGHRGVNQPVADTRTGRIAVTSHNHG
FAVRAPLTGTTDTPYGRVEVSHVALNDDVVEGLTCLDVPAFSVQFHPEAAPGPHDAQGLFDRFCGLMAAGRRKRGEG
>Mature_396_residues
TGLDRQPAPPEREQARRGPGAPRRAVLMLEDGRSFAGDAFGSVGEAFGEAVFSTGMTGYQETLTDPSFHRQVVIMTAPHI
GNTGVNDTDYESDRIQVAGFVVRDPSRLASNWRAQRTLDDELENAGVVGISGVDTRALTRHLRERGAMRCGVSSTDTDLD
SLLDRVRESPEMVGADLAPEVSTDKPYVVEARSGLPLFTVAALDLGIKRNTPLSMAALGCEVHVLPARSTAAELLALSPD
GVFLSNGPGDPARADYAVETLTGVLEAGVPVFGICFGNQVLARALGFETYKLTYGHRGVNQPVADTRTGRIAVTSHNHGF
AVRAPLTGTTDTPYGRVEVSHVALNDDVVEGLTCLDVPAFSVQFHPEAAPGPHDAQGLFDRFCGLMAAGRRKRGEG

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=371, Percent_Identity=42.0485175202156, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI169790915, Length=378, Percent_Identity=35.1851851851852, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI21361331, Length=378, Percent_Identity=35.1851851851852, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1786215, Length=383, Percent_Identity=44.6475195822454, Blast_Score=322, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI193204318, Length=375, Percent_Identity=38.6666666666667, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=37.9134860050891, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=382, Percent_Identity=37.434554973822, Blast_Score=229, Evalue=7e-61,
Organism=Drosophila melanogaster, GI45555749, Length=376, Percent_Identity=38.8297872340425, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24642586, Length=376, Percent_Identity=38.8297872340425, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42294; Mature: 42163

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLDRQPAPPEREQARRGPGAPRRAVLMLEDGRSFAGDAFGSVGEAFGEAVFSTGMTGY
CCCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCH
QETLTDPSFHRQVVIMTAPHIGNTGVNDTDYESDRIQVAGFVVRDPSRLASNWRAQRTLD
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHCCHH
DELENAGVVGISGVDTRALTRHLRERGAMRCGVSSTDTDLDSLLDRVRESPEMVGADLAP
HHHHCCCEEEECCCCHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCHHHCCCCCCC
EVSTDKPYVVEARSGLPLFTVAALDLGIKRNTPLSMAALGCEVHVLPARSTAAELLALSP
CCCCCCCEEEEECCCCCEEEEHHHHHCCCCCCCCEEEEECCEEEEEECCCCHHHEEEECC
DGVFLSNGPGDPARADYAVETLTGVLEAGVPVFGICFGNQVLARALGFETYKLTYGHRGV
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCC
NQPVADTRTGRIAVTSHNHGFAVRAPLTGTTDTPYGRVEVSHVALNDDVVEGLTCLDVPA
CCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEEECCHHHCCEEEECCCC
FSVQFHPEAAPGPHDAQGLFDRFCGLMAAGRRKRGEG
EEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TGLDRQPAPPEREQARRGPGAPRRAVLMLEDGRSFAGDAFGSVGEAFGEAVFSTGMTGY
CCCCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCH
QETLTDPSFHRQVVIMTAPHIGNTGVNDTDYESDRIQVAGFVVRDPSRLASNWRAQRTLD
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHCCHH
DELENAGVVGISGVDTRALTRHLRERGAMRCGVSSTDTDLDSLLDRVRESPEMVGADLAP
HHHHCCCEEEECCCCHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCHHHCCCCCCC
EVSTDKPYVVEARSGLPLFTVAALDLGIKRNTPLSMAALGCEVHVLPARSTAAELLALSP
CCCCCCCEEEEECCCCCEEEEHHHHHCCCCCCCCEEEEECCEEEEEECCCCHHHEEEECC
DGVFLSNGPGDPARADYAVETLTGVLEAGVPVFGICFGNQVLARALGFETYKLTYGHRGV
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCC
NQPVADTRTGRIAVTSHNHGFAVRAPLTGTTDTPYGRVEVSHVALNDDVVEGLTCLDVPA
CCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEEECCHHHCCEEEECCCC
FSVQFHPEAAPGPHDAQGLFDRFCGLMAAGRRKRGEG
EEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]