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The map label for this gene is 158313440

Identifier: 158313440

GI number: 158313440

Start: 1918421

End: 1919995

Strand: Direct

Name: 158313440

Synonym: Franean1_1603

Alternate gene names: NA

Gene position: 1918421-1919995 (Clockwise)

Preceding gene: 158313436

Following gene: 158313442

Centisome position: 21.36

GC content: 74.54

Gene sequence:

>1575_bases
ATGGGTCTGCTCGACGCGATCACGAACATGGGTCACACACCGGCGCACGTCCGCCCGCTGACCCCGGGCGACCGCGCCTA
TCTCGCCTTCGTCCGGCGCAATCCCGGTGAGCACCAGGACATCGGCGCGCTGCTGCACTTCGACGGCCCCCCGCTGGACC
TGCCGGCCCTGCGGGCCCACGTGGCCGAGCGGCTGCGCGACCCGCGGGCCCGGATGCTGACCGACCGTCTCGACACAGTG
CGGGTCTGGTCCCCCGAGCGGGGCGCGTCGGCCGAGGAGACCCGCTGGGTCTCCGACCCCGACATGAACCTCGACGACCA
TGTCGTCGCCTTCGACCTGCCCCCCGCAGACGGGGGCGCCGCGGCGGGGCAGTCCGCCGACGCGTCCCACGACGCCCGGC
TGCGGGCCGCCGTCGACGCGATCGTCGCCCGGCCGATCGACCTCACCCGGCCGCCGTGGATGCTCTACCTCCTGCGTGAC
CCGACCCCGGGGGCGACTGGTACCGCGCTGGTCTACCGCTCCAGCCACGTCCAGCAGGACGGCTTCGCGCTCTACCGGGT
GATGTACCTGCTGTTCGGTGAGAGCGACGAGGTCGATCTCGGGCTGGCGCCGACGATCCGCCGCCCCCGCCCGGCCGACT
ACGCCCGGTTCGTCGGCCGCGGGATCTCCTGCCTGCTGCCGACCCGGCGCCTCGAGTCCTGGGGCGGCCCACCGAGCGGC
CCGGCGAGACTTACCTGGGTGACCACGGAGCTCGCCACGCTGCGCGCGGTGGCCCGCCAGCACGGCGTCACCGTGAACGA
CGTCTACCTGGCGGCGCTCGCCGGGGCGCTGCGCGCCTGGTCGCTGCCGGAGTGGGAGCGCAGCGGCCGTCAGCTGCACG
CGCTGATGCCCGTCAGCATCCGCTCCGCGGCCGAACAGGACGTCCTGTCGAACCACAGCACCGGGGCGCGCGTCCCGCTG
TTCTGCGGTGAGCCCGACCCGGCCCGGCGGGTGGCCATGATCGCGGCGGAGACCCGCCGGATGAAGCAGGGCGGGCTGGG
CCTGGTGGAGCGCCAGCACTTCCCGCTCATGGCGGCGAAGGCCTCCCAACGCATGCTCGCGAACGTCGGCAGCTATCCGG
CCCAGATCAACAAGATGGCGCTGGTGGCGACCAACGCCCGGTCGATCCGCGGCCCGCTCTCCATCGCCGGCCGCCGGATG
ACCGGGCTGATCGGCATGGGCCCGCTGCTCGTCGGACGTCAGCACCTGGCCGTGGCGATGTTCGGCGTGGACGACCGGGT
CGGGGTCACGTTCGTGGCCAGCGAGAGCGTCCCGGACCACGCCCGGCTTGCCGACCTGTGGCTGGCCGAGCTGGCCGCGC
TGGGCCGGTCCGACTCGCCCGTCGGGGTGAGCGTGCCGACCCAGCGCCTGTCCTCGGCATCGGCCGCGGTGACGGCCGTC
ATGGCCGGCGCGGGGCCCGGCGCTGTCTCGGGTGCCGTCTCAGGTGCGGTGACCGGTGCCGTCCGGACCGAGGTCGGCGC
CCTCATCCGCCCCTGGCGCCGCCGCCCCACCACCCCGGCAGCCCCCGCCATGTAA

Upstream 100 bases:

>100_bases
TAGGTATCTCTCGCGCACAGTTGCGCCGATCCGCCAGGACCGTTGATCAGTTGGTTTCGGCCTGTACCCAACGCCCGCGG
ACGGGTTGGGGAAGCGTCTG

Downstream 100 bases:

>100_bases
CTGTCACGTGACCTGTCGGAACCGGCCCCGCGTCGGGCCCAGTGAAGCGTGGCGGGTGAGTGAGTCCATGGACTCACTCA
CCCGCCACGCACCTTCCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1425/MT1468 [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MGLLDAITNMGHTPAHVRPLTPGDRAYLAFVRRNPGEHQDIGALLHFDGPPLDLPALRAHVAERLRDPRARMLTDRLDTV
RVWSPERGASAEETRWVSDPDMNLDDHVVAFDLPPADGGAAAGQSADASHDARLRAAVDAIVARPIDLTRPPWMLYLLRD
PTPGATGTALVYRSSHVQQDGFALYRVMYLLFGESDEVDLGLAPTIRRPRPADYARFVGRGISCLLPTRRLESWGGPPSG
PARLTWVTTELATLRAVARQHGVTVNDVYLAALAGALRAWSLPEWERSGRQLHALMPVSIRSAAEQDVLSNHSTGARVPL
FCGEPDPARRVAMIAAETRRMKQGGLGLVERQHFPLMAAKASQRMLANVGSYPAQINKMALVATNARSIRGPLSIAGRRM
TGLIGMGPLLVGRQHLAVAMFGVDDRVGVTFVASESVPDHARLADLWLAELAALGRSDSPVGVSVPTQRLSSASAAVTAV
MAGAGPGAVSGAVSGAVTGAVRTEVGALIRPWRRRPTTPAAPAM

Sequences:

>Translated_524_residues
MGLLDAITNMGHTPAHVRPLTPGDRAYLAFVRRNPGEHQDIGALLHFDGPPLDLPALRAHVAERLRDPRARMLTDRLDTV
RVWSPERGASAEETRWVSDPDMNLDDHVVAFDLPPADGGAAAGQSADASHDARLRAAVDAIVARPIDLTRPPWMLYLLRD
PTPGATGTALVYRSSHVQQDGFALYRVMYLLFGESDEVDLGLAPTIRRPRPADYARFVGRGISCLLPTRRLESWGGPPSG
PARLTWVTTELATLRAVARQHGVTVNDVYLAALAGALRAWSLPEWERSGRQLHALMPVSIRSAAEQDVLSNHSTGARVPL
FCGEPDPARRVAMIAAETRRMKQGGLGLVERQHFPLMAAKASQRMLANVGSYPAQINKMALVATNARSIRGPLSIAGRRM
TGLIGMGPLLVGRQHLAVAMFGVDDRVGVTFVASESVPDHARLADLWLAELAALGRSDSPVGVSVPTQRLSSASAAVTAV
MAGAGPGAVSGAVSGAVTGAVRTEVGALIRPWRRRPTTPAAPAM
>Mature_523_residues
GLLDAITNMGHTPAHVRPLTPGDRAYLAFVRRNPGEHQDIGALLHFDGPPLDLPALRAHVAERLRDPRARMLTDRLDTVR
VWSPERGASAEETRWVSDPDMNLDDHVVAFDLPPADGGAAAGQSADASHDARLRAAVDAIVARPIDLTRPPWMLYLLRDP
TPGATGTALVYRSSHVQQDGFALYRVMYLLFGESDEVDLGLAPTIRRPRPADYARFVGRGISCLLPTRRLESWGGPPSGP
ARLTWVTTELATLRAVARQHGVTVNDVYLAALAGALRAWSLPEWERSGRQLHALMPVSIRSAAEQDVLSNHSTGARVPLF
CGEPDPARRVAMIAAETRRMKQGGLGLVERQHFPLMAAKASQRMLANVGSYPAQINKMALVATNARSIRGPLSIAGRRMT
GLIGMGPLLVGRQHLAVAMFGVDDRVGVTFVASESVPDHARLADLWLAELAALGRSDSPVGVSVPTQRLSSASAAVTAVM
AGAGPGAVSGAVSGAVTGAVRTEVGALIRPWRRRPTTPAAPAM

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 56053; Mature: 55922

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLDAITNMGHTPAHVRPLTPGDRAYLAFVRRNPGEHQDIGALLHFDGPPLDLPALRAH
CCHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHH
VAERLRDPRARMLTDRLDTVRVWSPERGASAEETRWVSDPDMNLDDHVVAFDLPPADGGA
HHHHHCCCHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCC
AAGQSADASHDARLRAAVDAIVARPIDLTRPPWMLYLLRDPTPGATGTALVYRSSHVQQD
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCC
GFALYRVMYLLFGESDEVDLGLAPTIRRPRPADYARFVGRGISCLLPTRRLESWGGPPSG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCHHHHHHCCCCCCC
PARLTWVTTELATLRAVARQHGVTVNDVYLAALAGALRAWSLPEWERSGRQLHALMPVSI
CCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCHHH
RSAAEQDVLSNHSTGARVPLFCGEPDPARRVAMIAAETRRMKQGGLGLVERQHFPLMAAK
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHH
ASQRMLANVGSYPAQINKMALVATNARSIRGPLSIAGRRMTGLIGMGPLLVGRQHLAVAM
HHHHHHHHCCCCCHHHCCEEEEEECCHHHCCCHHHCCCHHHHHHCCCHHHHCCCEEEEEE
FGVDDRVGVTFVASESVPDHARLADLWLAELAALGRSDSPVGVSVPTQRLSSASAAVTAV
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
MAGAGPGAVSGAVSGAVTGAVRTEVGALIRPWRRRPTTPAAPAM
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GLLDAITNMGHTPAHVRPLTPGDRAYLAFVRRNPGEHQDIGALLHFDGPPLDLPALRAH
CHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHH
VAERLRDPRARMLTDRLDTVRVWSPERGASAEETRWVSDPDMNLDDHVVAFDLPPADGGA
HHHHHCCCHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCC
AAGQSADASHDARLRAAVDAIVARPIDLTRPPWMLYLLRDPTPGATGTALVYRSSHVQQD
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCC
GFALYRVMYLLFGESDEVDLGLAPTIRRPRPADYARFVGRGISCLLPTRRLESWGGPPSG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCHHHHHHCCCCCCC
PARLTWVTTELATLRAVARQHGVTVNDVYLAALAGALRAWSLPEWERSGRQLHALMPVSI
CCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEECCHHH
RSAAEQDVLSNHSTGARVPLFCGEPDPARRVAMIAAETRRMKQGGLGLVERQHFPLMAAK
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHH
ASQRMLANVGSYPAQINKMALVATNARSIRGPLSIAGRRMTGLIGMGPLLVGRQHLAVAM
HHHHHHHHCCCCCHHHCCEEEEEECCHHHCCCHHHCCCHHHHHHCCCHHHHCCCEEEEEE
FGVDDRVGVTFVASESVPDHARLADLWLAELAALGRSDSPVGVSVPTQRLSSASAAVTAV
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
MAGAGPGAVSGAVSGAVTGAVRTEVGALIRPWRRRPTTPAAPAM
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]