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The map label for this gene is grpE2 [H]

Identifier: 158313407

GI number: 158313407

Start: 1876757

End: 1877242

Strand: Direct

Name: grpE2 [H]

Synonym: Franean1_1570

Alternate gene names: 158313407

Gene position: 1876757-1877242 (Clockwise)

Preceding gene: 158313406

Following gene: 158313408

Centisome position: 20.89

GC content: 76.95

Gene sequence:

>486_bases
ATGATGACTGACCACCCCGACGGCGCGGACCTGGCGGCCGAGCTGGAGGAGTCGCAGGCCAGACATCTGCGGACGCTGGC
CGACTTCGACAACTACCGCCGCCGGACCGGACGGGAGATCAGCGCCGCCATGGCGGCCGAGCGGGACCGCGTCGTCCTCG
CCTGGCTGCCGGTGCTCGACCACCTGGAGCTGGCCCTCGCCCACGCGGATGCCGACCCCGACTCCCTCGTCGACGGCGTG
CGCGGGGTGCGCCAGCTCGCGCTCGACGCGCTGCGGATCTCCGGCGTCGCCCGGCTCGACGACGAGACCGGCCCCTTCGA
CCCGGCCCGGCACGAGGTCGGCGCCGTCGTCGACAGCGCCTCGACGCCGCAGCCGCCCCCGGCGGGCACCGTCGTCGAGG
TCCTGCGCCCCGGCGTCGAGGCCGGCGGGCGGGTGCTGCGGCCGGCGTCGGTCGCGGTGAGCGCCGGCCCGCGGACGGCG
ACATGA

Upstream 100 bases:

>100_bases
GCGAGTGGCACCGGTTCGGGCGCGAGTGGCACCGGTTCGGGAGCCGGCGGGCACGGCGTCGGCGGGGACGATGTGATCGA
CGCCGAGTTCACCACCCGCG

Downstream 100 bases:

>100_bases
GCGCCGGGACGACGGGACGCGCGAGCCCGGACTTCTACGAGCTGCTCGGCGTGCCGCGTGACGCCGACACCGACGCCATC
CAGCGTGCGTACCGCAAGCT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor 2 [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MMTDHPDGADLAAELEESQARHLRTLADFDNYRRRTGREISAAMAAERDRVVLAWLPVLDHLELALAHADADPDSLVDGV
RGVRQLALDALRISGVARLDDETGPFDPARHEVGAVVDSASTPQPPPAGTVVEVLRPGVEAGGRVLRPASVAVSAGPRTA
T

Sequences:

>Translated_161_residues
MMTDHPDGADLAAELEESQARHLRTLADFDNYRRRTGREISAAMAAERDRVVLAWLPVLDHLELALAHADADPDSLVDGV
RGVRQLALDALRISGVARLDDETGPFDPARHEVGAVVDSASTPQPPPAGTVVEVLRPGVEAGGRVLRPASVAVSAGPRTA
T
>Mature_161_residues
MMTDHPDGADLAAELEESQARHLRTLADFDNYRRRTGREISAAMAAERDRVVLAWLPVLDHLELALAHADADPDSLVDGV
RGVRQLALDALRISGVARLDDETGPFDPARHEVGAVVDSASTPQPPPAGTVVEVLRPGVEAGGRVLRPASVAVSAGPRTA
T

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=153, Percent_Identity=33.9869281045752, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 17066; Mature: 17066

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDHPDGADLAAELEESQARHLRTLADFDNYRRRTGREISAAMAAERDRVVLAWLPVLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEHHHHHHH
HLELALAHADADPDSLVDGVRGVRQLALDALRISGVARLDDETGPFDPARHEVGAVVDSA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHHHHHHCCC
STPQPPPAGTVVEVLRPGVEAGGRVLRPASVAVSAGPRTAT
CCCCCCCCCHHHHHHHCCHHCCCCEECCHHEEEECCCCCCC
>Mature Secondary Structure
MMTDHPDGADLAAELEESQARHLRTLADFDNYRRRTGREISAAMAAERDRVVLAWLPVLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEHHHHHHH
HLELALAHADADPDSLVDGVRGVRQLALDALRISGVARLDDETGPFDPARHEVGAVVDSA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCHHHHHHHHHHCCC
STPQPPPAGTVVEVLRPGVEAGGRVLRPASVAVSAGPRTAT
CCCCCCCCCHHHHHHHCCHHCCCCEECCHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]