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The map label for this gene is aspS [H]

Identifier: 158313365

GI number: 158313365

Start: 1829378

End: 1830679

Strand: Direct

Name: aspS [H]

Synonym: Franean1_1527

Alternate gene names: 158313365

Gene position: 1829378-1830679 (Clockwise)

Preceding gene: 158313362

Following gene: 158313366

Centisome position: 20.37

GC content: 58.53

Gene sequence:

>1302_bases
ATGGAAAGAACCTATACAACGGATCTTCCCAAGCGCGCGGGCGAGAAAGTGCATCTTGCGGGATGGGTCCACCGGCGCCG
CGTTCTGAAAAACGTGACATTTCTTGTCCTGCGCGACGCACGCGGCACAGCGCAAATCGTCGTGAACGAGCCCGACATTC
TCTCGAAAATCTCGGATGTCACCGAGGAATCCGTCGTAGAAATTGACGGACAAGCAGTAGCGAACCCACACGCACCCGGT
GGGGTGGAAGTCATTAACCCCGAGATAGGAATTCTCTCCGAGGCTGTTTCTCCGTTGCCTTTCGAGATTTTCCGACCGAA
CCTGGACGTGAGCCTTCCCGTACAGCTCGACCATGCATCGCTCGCGCTGAGGCACCCACGGCGGCAAGCAATATTCCGGC
TGGCTGCCGCATCCGTCGCGGGATTCAGGGAAGCCCTCACGAGCGAAGGGTTCACCGAGGTCCACACTCCAAAGATCGTC
GCATCCTCTACTGAGAGCGGAGCGAACGTCTTCGGCATAGACTATTTTGGATCGCGCGCCTACCTCGCCCAGAGCCCGCA
GTTCTATAAGCAAACTCTCGTTGGCGTGTTCGAGCGAGTATTTGAAGTAGCTCCGGTTTTTCGGGCCGAACCGCACGACA
CCGCAAGACATCTCGCAGAATACGTCTCCCTGGATGCCGAGATGGGATTCATTGAGGACCACCACACCGTCATAAAAATG
GTGAGTCTGGCGGTTAGGCACATGGTAGAGAAGGTGTCGGCTACTGACCCAGAGGAGTCGATACTTGCCGACCAGACGTT
GCCCCGTATCCCCGAGCAGATACCGATCATCCATTTCTCTGACGCTCAGGAGATGATCCAGCGAGCAACCGGCGAACAGG
TGATTGGTGAGCCTGACCTCGCCCCGGCGCACGAACGGTGGCTCGGAGAGTGGGCGCTCCACGAGTTCGGTAGCGACTTT
CTTTTCGTAACGGGCTACCCCATGCGCAAAAGGCCCTTCTACACGCACCCGGACCCCGCGCGACCCGAGTTCTCCAACAG
TTTCGACCTTCTGTTCCGAGGATTGGAACTCGTGACCGGGGGGCAACGGCTCCACCGATACACCGATTACGTACAGGCAA
TTACCTTGCGCGGCGAAGACCCTGAGAAGTACCGGGCGTACATTGACACTTTCCGGTACGGCATGCCGCCCCACGGCGGT
TTCGCACTCGGCCTGGAAAGATGGACCGCCCGACTTACCGGCGCGGCAAACGTTCGCGAGGTCACTCTGTTTCCCCGGGA
CTTGCACAGGCTCACTCCGTAG

Upstream 100 bases:

>100_bases
CGACGAGGCCGCTTGTTCCGACAAACGGCGAAGTGCGGTGGCACCGCGAGAAGCCCCTCGCCCGCACCTTCGGGGACGAA
GCTGCCGAGAGGGAACAACG

Downstream 100 bases:

>100_bases
TAGCCGGTGGATTTTCCCACTCACGACCCTCTTTCGTGCTGAGCGGGTCTCCTCCGGATTGGCAACACTCGGCGGAGTAT
CACGGAGACATGCCATCGCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MERTYTTDLPKRAGEKVHLAGWVHRRRVLKNVTFLVLRDARGTAQIVVNEPDILSKISDVTEESVVEIDGQAVANPHAPG
GVEVINPEIGILSEAVSPLPFEIFRPNLDVSLPVQLDHASLALRHPRRQAIFRLAAASVAGFREALTSEGFTEVHTPKIV
ASSTESGANVFGIDYFGSRAYLAQSPQFYKQTLVGVFERVFEVAPVFRAEPHDTARHLAEYVSLDAEMGFIEDHHTVIKM
VSLAVRHMVEKVSATDPEESILADQTLPRIPEQIPIIHFSDAQEMIQRATGEQVIGEPDLAPAHERWLGEWALHEFGSDF
LFVTGYPMRKRPFYTHPDPARPEFSNSFDLLFRGLELVTGGQRLHRYTDYVQAITLRGEDPEKYRAYIDTFRYGMPPHGG
FALGLERWTARLTGAANVREVTLFPRDLHRLTP

Sequences:

>Translated_433_residues
MERTYTTDLPKRAGEKVHLAGWVHRRRVLKNVTFLVLRDARGTAQIVVNEPDILSKISDVTEESVVEIDGQAVANPHAPG
GVEVINPEIGILSEAVSPLPFEIFRPNLDVSLPVQLDHASLALRHPRRQAIFRLAAASVAGFREALTSEGFTEVHTPKIV
ASSTESGANVFGIDYFGSRAYLAQSPQFYKQTLVGVFERVFEVAPVFRAEPHDTARHLAEYVSLDAEMGFIEDHHTVIKM
VSLAVRHMVEKVSATDPEESILADQTLPRIPEQIPIIHFSDAQEMIQRATGEQVIGEPDLAPAHERWLGEWALHEFGSDF
LFVTGYPMRKRPFYTHPDPARPEFSNSFDLLFRGLELVTGGQRLHRYTDYVQAITLRGEDPEKYRAYIDTFRYGMPPHGG
FALGLERWTARLTGAANVREVTLFPRDLHRLTP
>Mature_433_residues
MERTYTTDLPKRAGEKVHLAGWVHRRRVLKNVTFLVLRDARGTAQIVVNEPDILSKISDVTEESVVEIDGQAVANPHAPG
GVEVINPEIGILSEAVSPLPFEIFRPNLDVSLPVQLDHASLALRHPRRQAIFRLAAASVAGFREALTSEGFTEVHTPKIV
ASSTESGANVFGIDYFGSRAYLAQSPQFYKQTLVGVFERVFEVAPVFRAEPHDTARHLAEYVSLDAEMGFIEDHHTVIKM
VSLAVRHMVEKVSATDPEESILADQTLPRIPEQIPIIHFSDAQEMIQRATGEQVIGEPDLAPAHERWLGEWALHEFGSDF
LFVTGYPMRKRPFYTHPDPARPEFSNSFDLLFRGLELVTGGQRLHRYTDYVQAITLRGEDPEKYRAYIDTFRYGMPPHGG
FALGLERWTARLTGAANVREVTLFPRDLHRLTP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=449, Percent_Identity=35.8574610244989, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI4758762, Length=435, Percent_Identity=29.8850574712644, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI226491502, Length=444, Percent_Identity=26.1261261261261, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI40789249, Length=233, Percent_Identity=32.1888412017167, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1787161, Length=463, Percent_Identity=26.7818574514039, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790571, Length=447, Percent_Identity=23.0425055928412, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788173, Length=260, Percent_Identity=27.3076923076923, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=454, Percent_Identity=34.8017621145374, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI71984122, Length=439, Percent_Identity=31.4350797266515, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17556372, Length=300, Percent_Identity=27.3333333333333, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=298, Percent_Identity=28.1879194630872, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=338, Percent_Identity=40.2366863905325, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6321807, Length=448, Percent_Identity=30.5803571428571, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6319872, Length=486, Percent_Identity=24.8971193415638, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325153, Length=257, Percent_Identity=28.7937743190661, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17136276, Length=444, Percent_Identity=36.4864864864865, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI19921528, Length=433, Percent_Identity=29.0993071593533, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19922416, Length=465, Percent_Identity=21.9354838709677, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24584738, Length=248, Percent_Identity=31.8548387096774, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 48632; Mature: 48632

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTYTTDLPKRAGEKVHLAGWVHRRRVLKNVTFLVLRDARGTAQIVVNEPDILSKISDV
CCCCCCCCCHHHCCCEEEEHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCHHHHHHHHH
TEESVVEIDGQAVANPHAPGGVEVINPEIGILSEAVSPLPFEIFRPNLDVSLPVQLDHAS
HHHHHEEECCCEECCCCCCCCCEEECCCHHHHHHHCCCCCHHHCCCCCCEEEEEEECCHH
LALRHPRRQAIFRLAAASVAGFREALTSEGFTEVHTPKIVASSTESGANVFGIDYFGSRA
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCH
YLAQSPQFYKQTLVGVFERVFEVAPVFRAEPHDTARHLAEYVSLDAEMGFIEDHHTVIKM
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VSLAVRHMVEKVSATDPEESILADQTLPRIPEQIPIIHFSDAQEMIQRATGEQVIGEPDL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCC
APAHERWLGEWALHEFGSDFLFVTGYPMRKRPFYTHPDPARPEFSNSFDLLFRGLELVTG
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
GQRLHRYTDYVQAITLRGEDPEKYRAYIDTFRYGMPPHGGFALGLERWTARLTGAANVRE
HHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCEE
VTLFPRDLHRLTP
EEECHHHHHCCCC
>Mature Secondary Structure
MERTYTTDLPKRAGEKVHLAGWVHRRRVLKNVTFLVLRDARGTAQIVVNEPDILSKISDV
CCCCCCCCCHHHCCCEEEEHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCHHHHHHHHH
TEESVVEIDGQAVANPHAPGGVEVINPEIGILSEAVSPLPFEIFRPNLDVSLPVQLDHAS
HHHHHEEECCCEECCCCCCCCCEEECCCHHHHHHHCCCCCHHHCCCCCCEEEEEEECCHH
LALRHPRRQAIFRLAAASVAGFREALTSEGFTEVHTPKIVASSTESGANVFGIDYFGSRA
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCH
YLAQSPQFYKQTLVGVFERVFEVAPVFRAEPHDTARHLAEYVSLDAEMGFIEDHHTVIKM
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VSLAVRHMVEKVSATDPEESILADQTLPRIPEQIPIIHFSDAQEMIQRATGEQVIGEPDL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCC
APAHERWLGEWALHEFGSDFLFVTGYPMRKRPFYTHPDPARPEFSNSFDLLFRGLELVTG
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
GQRLHRYTDYVQAITLRGEDPEKYRAYIDTFRYGMPPHGGFALGLERWTARLTGAANVRE
HHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCEE
VTLFPRDLHRLTP
EEECHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]