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The map label for this gene is pcm [H]

Identifier: 158313185

GI number: 158313185

Start: 1607244

End: 1608446

Strand: Direct

Name: pcm [H]

Synonym: Franean1_1335

Alternate gene names: 158313185

Gene position: 1607244-1608446 (Clockwise)

Preceding gene: 158313184

Following gene: 158313186

Centisome position: 17.89

GC content: 72.9

Gene sequence:

>1203_bases
ATGACCACCCGCACCCCCGAGCAGCTCCGCGACGACCTGGTCGCCCACATCCGCCGCTGGGGCACCTTCCGCACCGGTCA
GGTCGAAGCCGCGTTCCGCACCGTGCCGCGGCATCTGTTCCTGCCCGACGTCGACCTGGAGACCGCCTACGGCACGCAGG
TCGTCGTGACCCGCCGCGCATCCGACGGCACGGCGCTGTCCTCGGCCTCGCAGCCCGACCTCGTCGCCGCCATGCTCGAA
CAGGCTGACGTCCACCCCGGTCACCGCATCCTGGAGATCGGCACCGCCACCGGCATCAACGCCGCTCTCCTGGCAGAACT
GGCCGGCCCGACCGGTCATGTGACCACCATCGAGATCGACGAGGACCTCATCGGCGGCGCCCGCACCGCGCTGGCGGCAG
CCAGCTACGACCAGGTCGACGTGATCCACGGGGACGGCGCGGTCGGCTACCCGGATGGGGCATCTTACGACCGGATCGTG
ATCACGGCGGGGGCGTTCGACCTTGCAGCAGCCTGGTGGGAGCAGCTCGCCCCCGCCGGCCGGATCGTCGTACCACTACG
CCTGCACGGAAGCGGCCTGACCCGCTCCCTCGCGTTCGACGCGGGCGAGCCAGGCCGGCTGGTCAGCCGCTCGGCGCTCG
TCTGCGGATTCATTCCGCTGCGCGGCGCCGGCGCCCACGCCGGCCGCACGCTGACCCTCGCGGACGGAGCTGTCCTGCGC
GTCGACGACCACGACCCCGTGGACGAGCCTGCGCTACGCGCCGCGGCAGACAGCCCTCCCCACGCGCTGTGGACCGGTCT
GACGATCCATGACCACGAACCGACCGCCCACCTCGACCTGTGGCTCGTCACCGCCGGCACCCGCTTCGCCCGCCTCGCCG
TCGACACCACCGCCCGCCACGACAGCCAGCTCGGCCCGGCGCGGCGCTGGGCCGGGGCCACCATCCACGACGGCACCACG
ATCGCCTACGTCACCCTGCGCCCACTCGCCCCCGACACCGACGAGCTCGGCGTCACCGCCCACGGTCCCCACGCGACCAC
CCTCGCCACGCAGCTCACCGACCTGCTCCATCACTGGCGAAAGGAAGGCCCCGCCGAGCCCGTCATCACCGCCCACCCCG
CAGGCACCCCCGACGACCAGCTCTCCGCTGGACACCGCATCGACCGGCCGAACAGCCGCCTCACCGTCCGCTGGCAGCCC
TGA

Upstream 100 bases:

>100_bases
GCATCCCCGCCGCCAACCAGGCGCAGCTGGCCCACACCGCCGCCCACATCGTCTTCCACGACAGCCACCCGGTCCCGACC
CCCGACGAACGGTAACCACG

Downstream 100 bases:

>100_bases
GAACGGGCGACCAGCGCCATGGCTGCCACCACGTCCACGGCCGCGACACCCTCGACGTTCGACATCGTCGCCGCGCGCTT
CCCCCTCGTTCCCCGCTCCC

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTTRTPEQLRDDLVAHIRRWGTFRTGQVEAAFRTVPRHLFLPDVDLETAYGTQVVVTRRASDGTALSSASQPDLVAAMLE
QADVHPGHRILEIGTATGINAALLAELAGPTGHVTTIEIDEDLIGGARTALAAASYDQVDVIHGDGAVGYPDGASYDRIV
ITAGAFDLAAAWWEQLAPAGRIVVPLRLHGSGLTRSLAFDAGEPGRLVSRSALVCGFIPLRGAGAHAGRTLTLADGAVLR
VDDHDPVDEPALRAAADSPPHALWTGLTIHDHEPTAHLDLWLVTAGTRFARLAVDTTARHDSQLGPARRWAGATIHDGTT
IAYVTLRPLAPDTDELGVTAHGPHATTLATQLTDLLHHWRKEGPAEPVITAHPAGTPDDQLSAGHRIDRPNSRLTVRWQP

Sequences:

>Translated_400_residues
MTTRTPEQLRDDLVAHIRRWGTFRTGQVEAAFRTVPRHLFLPDVDLETAYGTQVVVTRRASDGTALSSASQPDLVAAMLE
QADVHPGHRILEIGTATGINAALLAELAGPTGHVTTIEIDEDLIGGARTALAAASYDQVDVIHGDGAVGYPDGASYDRIV
ITAGAFDLAAAWWEQLAPAGRIVVPLRLHGSGLTRSLAFDAGEPGRLVSRSALVCGFIPLRGAGAHAGRTLTLADGAVLR
VDDHDPVDEPALRAAADSPPHALWTGLTIHDHEPTAHLDLWLVTAGTRFARLAVDTTARHDSQLGPARRWAGATIHDGTT
IAYVTLRPLAPDTDELGVTAHGPHATTLATQLTDLLHHWRKEGPAEPVITAHPAGTPDDQLSAGHRIDRPNSRLTVRWQP
>Mature_399_residues
TTRTPEQLRDDLVAHIRRWGTFRTGQVEAAFRTVPRHLFLPDVDLETAYGTQVVVTRRASDGTALSSASQPDLVAAMLEQ
ADVHPGHRILEIGTATGINAALLAELAGPTGHVTTIEIDEDLIGGARTALAAASYDQVDVIHGDGAVGYPDGASYDRIVI
TAGAFDLAAAWWEQLAPAGRIVVPLRLHGSGLTRSLAFDAGEPGRLVSRSALVCGFIPLRGAGAHAGRTLTLADGAVLRV
DDHDPVDEPALRAAADSPPHALWTGLTIHDHEPTAHLDLWLVTAGTRFARLAVDTTARHDSQLGPARRWAGATIHDGTTI
AYVTLRPLAPDTDELGVTAHGPHATTLATQLTDLLHHWRKEGPAEPVITAHPAGTPDDQLSAGHRIDRPNSRLTVRWQP

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=191, Percent_Identity=31.413612565445, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 42600; Mature: 42469

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRTPEQLRDDLVAHIRRWGTFRTGQVEAAFRTVPRHLFLPDVDLETAYGTQVVVTRRA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCCEEEEEEEC
SDGTALSSASQPDLVAAMLEQADVHPGHRILEIGTATGINAALLAELAGPTGHVTTIEID
CCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEC
EDLIGGARTALAAASYDQVDVIHGDGAVGYPDGASYDRIVITAGAFDLAAAWWEQLAPAG
HHHCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCC
RIVVPLRLHGSGLTRSLAFDAGEPGRLVSRSALVCGFIPLRGAGAHAGRTLTLADGAVLR
EEEEEEEECCCCCCEEEEECCCCCCCHHHCCEEEEEEEECCCCCCCCCCEEEEECCEEEE
VDDHDPVDEPALRAAADSPPHALWTGLTIHDHEPTAHLDLWLVTAGTRFARLAVDTTARH
ECCCCCCCCHHHHHCCCCCCHHEEECCEEECCCCCCEEEEEEEECCCEEEEEEEECCCCC
DSQLGPARRWAGATIHDGTTIAYVTLRPLAPDTDELGVTAHGPHATTLATQLTDLLHHWR
CCCCCCHHHHCCCEEECCCEEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
KEGPAEPVITAHPAGTPDDQLSAGHRIDRPNSRLTVRWQP
HCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
TTRTPEQLRDDLVAHIRRWGTFRTGQVEAAFRTVPRHLFLPDVDLETAYGTQVVVTRRA
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCCEEEEEEEC
SDGTALSSASQPDLVAAMLEQADVHPGHRILEIGTATGINAALLAELAGPTGHVTTIEID
CCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEEEC
EDLIGGARTALAAASYDQVDVIHGDGAVGYPDGASYDRIVITAGAFDLAAAWWEQLAPAG
HHHCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCC
RIVVPLRLHGSGLTRSLAFDAGEPGRLVSRSALVCGFIPLRGAGAHAGRTLTLADGAVLR
EEEEEEEECCCCCCEEEEECCCCCCCHHHCCEEEEEEEECCCCCCCCCCEEEEECCEEEE
VDDHDPVDEPALRAAADSPPHALWTGLTIHDHEPTAHLDLWLVTAGTRFARLAVDTTARH
ECCCCCCCCHHHHHCCCCCCHHEEECCEEECCCCCCEEEEEEEECCCEEEEEEEECCCCC
DSQLGPARRWAGATIHDGTTIAYVTLRPLAPDTDELGVTAHGPHATTLATQLTDLLHHWR
CCCCCCHHHHCCCEEECCCEEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
KEGPAEPVITAHPAGTPDDQLSAGHRIDRPNSRLTVRWQP
HCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]