Click here to switch to the map view.

The map label for this gene is rffG [C]

Identifier: 158313122

GI number: 158313122

Start: 1527878

End: 1528849

Strand: Direct

Name: rffG [C]

Synonym: Franean1_1270

Alternate gene names: 158313122

Gene position: 1527878-1528849 (Clockwise)

Preceding gene: 158313112

Following gene: 158313124

Centisome position: 17.01

GC content: 74.49

Gene sequence:

>972_bases
ATGAGGGTCGTGGTGACCGGGGGCGCCGGGTTCATCGGTGCCCACCTGACTCGTGCGCTGCTGGCCCGTGGCTGCGAGGT
GGTCGTCGTCGACGACCTCAGCACCGGAGCCCGGTCGAACCTGATCGGCCTGCCCGCGCGCCTCGTCCTCGGCAGCGTCA
CCGACCGGGAGCTGCTGGAGGACGCCTGCACCGGGGCGGACAGCGTCGTCCATCTGGCGGCGCGGCCCTCCGTCGAGCGT
TCGCTGCTCGACCCGCTGGCCACCCACCACGTCAACGCGACCGGCACACTGACCGTGCTCGACGTGGCACAGCGGGGCGA
GACCCACGTGGTGGTCGTCTCCTCCGCTCTGGTATACGGGACGTCCGGCGGCCGCTCCCAGAGCGAGGACGACCCGCCGC
GTCCGACCAGCCCGTACGCGGCCAGCGCGCTGGCGGCCGAGGGGTACGCCCTGGCGCACCAGGCGAGCTTCGGGCTGCCG
GTCCTGGTCGCGCGGCTGTTCAACGTCTACGGCCCGTACCAGCCGGCCCGGCACGCCCACGCCGCTGTCGTGCCGTCGTT
CATCGACGCCGCGCTGCGTGGACGTCCGCTGCCGGTGCACGGCGACGGCCGGCAGACCAGGGACTTCACCTACGTCGAGG
CGGTCGCCGGGCTGCTGGCCGACGCGGCCTGCCGTCGGCTGGCCCACCCCGGGCCGGTGAACCTGGCGTTCGGGACGAGC
ACCGACCTGCTGGCGTTGGTCGCCCACCTCGAGGACATCCTCGGCCGCCGGCTGGACGTGGCGCACGGCGCGCCGCGCGC
CGGGGACGTCCGCGACTCGCGCGCCCGCCCGGACGCCATGCACGCGCTGTTCGGCTCCGTCGAGCGCTCTGACCTGCGGG
CGACCCTGGAGGAGACCGTGTACTGGTACCAGGAACGCCTCGGCGAGGCCCCCGGCGCCGCGCCCAGATCCGCGGACACC
ACCCACCACTGA

Upstream 100 bases:

>100_bases
ATGCGCCGTATCGCCGACCGTTTCCCGCAACTCGATTACTATCAGCATCTGATCGGTTGCTTATCGTTGCCTGATGTGAT
CGTGGGGAGTGATTCGGACG

Downstream 100 bases:

>100_bases
CCGTCACCACCGACTCACCCGGTCCGCGCCTCGGTCGGTCCGTCCTGACGCGGCCCGTCCTGACGCGGCCCGGTCTGGCG
CGGCTCGTCCTGACCTGGAT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MRVVVTGGAGFIGAHLTRALLARGCEVVVVDDLSTGARSNLIGLPARLVLGSVTDRELLEDACTGADSVVHLAARPSVER
SLLDPLATHHVNATGTLTVLDVAQRGETHVVVVSSALVYGTSGGRSQSEDDPPRPTSPYAASALAAEGYALAHQASFGLP
VLVARLFNVYGPYQPARHAHAAVVPSFIDAALRGRPLPVHGDGRQTRDFTYVEAVAGLLADAACRRLAHPGPVNLAFGTS
TDLLALVAHLEDILGRRLDVAHGAPRAGDVRDSRARPDAMHALFGSVERSDLRATLEETVYWYQERLGEAPGAAPRSADT
THH

Sequences:

>Translated_323_residues
MRVVVTGGAGFIGAHLTRALLARGCEVVVVDDLSTGARSNLIGLPARLVLGSVTDRELLEDACTGADSVVHLAARPSVER
SLLDPLATHHVNATGTLTVLDVAQRGETHVVVVSSALVYGTSGGRSQSEDDPPRPTSPYAASALAAEGYALAHQASFGLP
VLVARLFNVYGPYQPARHAHAAVVPSFIDAALRGRPLPVHGDGRQTRDFTYVEAVAGLLADAACRRLAHPGPVNLAFGTS
TDLLALVAHLEDILGRRLDVAHGAPRAGDVRDSRARPDAMHALFGSVERSDLRATLEETVYWYQERLGEAPGAAPRSADT
THH
>Mature_323_residues
MRVVVTGGAGFIGAHLTRALLARGCEVVVVDDLSTGARSNLIGLPARLVLGSVTDRELLEDACTGADSVVHLAARPSVER
SLLDPLATHHVNATGTLTVLDVAQRGETHVVVVSSALVYGTSGGRSQSEDDPPRPTSPYAASALAAEGYALAHQASFGLP
VLVARLFNVYGPYQPARHAHAAVVPSFIDAALRGRPLPVHGDGRQTRDFTYVEAVAGLLADAACRRLAHPGPVNLAFGTS
TDLLALVAHLEDILGRRLDVAHGAPRAGDVRDSRARPDAMHALFGSVERSDLRATLEETVYWYQERLGEAPGAAPRSADT
THH

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=317, Percent_Identity=30.5993690851735, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=27.9141104294479, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI193211614, Length=221, Percent_Identity=29.8642533936652, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI8393516, Length=221, Percent_Identity=29.8642533936652, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI56237023, Length=348, Percent_Identity=26.1494252873563, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI56118217, Length=348, Percent_Identity=26.1494252873563, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI189083684, Length=348, Percent_Identity=26.1494252873563, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI48994969, Length=233, Percent_Identity=35.1931330472103, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=27.1676300578035, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786974, Length=296, Percent_Identity=27.027027027027, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=291, Percent_Identity=28.8659793814433, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17568069, Length=258, Percent_Identity=30.2325581395349, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115532424, Length=289, Percent_Identity=28.0276816608997, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=342, Percent_Identity=25.1461988304094, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=340, Percent_Identity=25, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=359, Percent_Identity=27.8551532033426, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356223, Length=322, Percent_Identity=28.8819875776398, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19923002, Length=338, Percent_Identity=27.2189349112426, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 34018; Mature: 34018

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVVTGGAGFIGAHLTRALLARGCEVVVVDDLSTGARSNLIGLPARLVLGSVTDRELLE
CEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCHHHHHH
DACTGADSVVHLAARPSVERSLLDPLATHHVNATGTLTVLDVAQRGETHVVVVSSALVYG
HHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCEEEEEEEEEEEEE
TSGGRSQSEDDPPRPTSPYAASALAAEGYALAHQASFGLPVLVARLFNVYGPYQPARHAH
CCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
AAVVPSFIDAALRGRPLPVHGDGRQTRDFTYVEAVAGLLADAACRRLAHPGPVNLAFGTS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TDLLALVAHLEDILGRRLDVAHGAPRAGDVRDSRARPDAMHALFGSVERSDLRATLEETV
HHHHHHHHHHHHHHHHHEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YWYQERLGEAPGAAPRSADTTHH
HHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVVVTGGAGFIGAHLTRALLARGCEVVVVDDLSTGARSNLIGLPARLVLGSVTDRELLE
CEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCHHHHHH
DACTGADSVVHLAARPSVERSLLDPLATHHVNATGTLTVLDVAQRGETHVVVVSSALVYG
HHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCEEEEEEEEEEEEE
TSGGRSQSEDDPPRPTSPYAASALAAEGYALAHQASFGLPVLVARLFNVYGPYQPARHAH
CCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
AAVVPSFIDAALRGRPLPVHGDGRQTRDFTYVEAVAGLLADAACRRLAHPGPVNLAFGTS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TDLLALVAHLEDILGRRLDVAHGAPRAGDVRDSRARPDAMHALFGSVERSDLRATLEETV
HHHHHHHHHHHHHHHHHEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YWYQERLGEAPGAAPRSADTTHH
HHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]