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The map label for this gene is miaA

Identifier: 158313080

GI number: 158313080

Start: 1469469

End: 1470425

Strand: Direct

Name: miaA

Synonym: Franean1_1228

Alternate gene names: 158313080

Gene position: 1469469-1470425 (Clockwise)

Preceding gene: 158313079

Following gene: 158313081

Centisome position: 16.36

GC content: 71.37

Gene sequence:

>957_bases
GTGACCGCGTCCCGTGACGGCGCCGGCCTGACCAACGGTGCCACCCGAGCCGATCAGGCCGGGCCGGTCATCGCGGTCGT
CGGCCCGACCGGCGCCGGTAAGAGCGACCTGGCGGTCGAGATCGCCCTCGAGCTCGGTGGGGAGATCGTCAACGCCGACT
CGATGCAGCTCTACCGGGGCATGGACATCGGCACGGCGAAGATCCCGATGGTCGAGCGCCGCGGCGTGCCCCACCATCTG
CTGGACGTCTGGGAGATCACCCGCGCCGCGGACGTCGCCAGCTACCAGGCCGACGCCCGGCGGGTCGTCAACGGCCTGCT
CGCCGCCGGCCGGGTGCCGATCCTGGTCGGCGGATCTGGCCTGTACGTGCGGGCGGTTCTGGACGATTTGTCATTTCCCG
GCACCGACCCGGCCGTCCGCGCCCGGCTCGAGCGCGAGCTCGCCGAGGTCGGGCCGGGGCCGCTGCACGAACGGCTGACG
GGGCTGGCGCCGACCGCCGCCGCGGCGATCCTGCCCGGCAACGGGCGGCGGATCGTGCGGGCGCTCGAGGTCGTCGAGCT
CACCGGCACATTCGAGGCCACTTTGCCCGAATATCGATCGATTTATGACGTCGTTCAGGTCGGCGTGGACCGCCCGGACC
TGGACACGCGGATCGCCGACCGGGTGGAGCTGATGTGGCGCGCGGGCTTCGTCGACGAGGTCGCGGCGCTCGCGGAGGCG
GGGCTGCGCGACGGGCGGACCGCCTCGCGTGCCCTCGGATACGCGCAGGTCCTGGCCGTCCTCGACGGAGCAATGGACTC
CATGGACGCGGCCAAACAGGCCACAACAACGGCCACCAGGCGCTTCGCTCGTCGGCAAAGATCCTGGTTCCGGCGTGATC
CGCGGATATCCTGGCTGGAATTTCCGGATGTCGCCGCGGCGCTCGCCGAGGTACGACGTCTGGTGGGTTCGCTCTGA

Upstream 100 bases:

>100_bases
ACGCCCCGTTCGGGGTCGGCTACCTGGTCGTCGGATGGGAGCGCGCGGACGGCACCCGGGTCGACCGGCAGGAGAAGCGG
GCCACCGCGCAGAGCGGCCG

Downstream 100 bases:

>100_bases
TATCGGGTTCGCTCTGACGTGTGGCCGGCCCAGCGCCGCGGGACCTTCGCCCGACCCGTCGGCCCGGGTCCCGGCCCATC
CCGGCGTCTGGAGAGTTCGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTASRDGAGLTNGATRADQAGPVIAVVGPTGAGKSDLAVEIALELGGEIVNADSMQLYRGMDIGTAKIPMVERRGVPHHL
LDVWEITRAADVASYQADARRVVNGLLAAGRVPILVGGSGLYVRAVLDDLSFPGTDPAVRARLERELAEVGPGPLHERLT
GLAPTAAAAILPGNGRRIVRALEVVELTGTFEATLPEYRSIYDVVQVGVDRPDLDTRIADRVELMWRAGFVDEVAALAEA
GLRDGRTASRALGYAQVLAVLDGAMDSMDAAKQATTTATRRFARRQRSWFRRDPRISWLEFPDVAAALAEVRRLVGSL

Sequences:

>Translated_318_residues
MTASRDGAGLTNGATRADQAGPVIAVVGPTGAGKSDLAVEIALELGGEIVNADSMQLYRGMDIGTAKIPMVERRGVPHHL
LDVWEITRAADVASYQADARRVVNGLLAAGRVPILVGGSGLYVRAVLDDLSFPGTDPAVRARLERELAEVGPGPLHERLT
GLAPTAAAAILPGNGRRIVRALEVVELTGTFEATLPEYRSIYDVVQVGVDRPDLDTRIADRVELMWRAGFVDEVAALAEA
GLRDGRTASRALGYAQVLAVLDGAMDSMDAAKQATTTATRRFARRQRSWFRRDPRISWLEFPDVAAALAEVRRLVGSL
>Mature_317_residues
TASRDGAGLTNGATRADQAGPVIAVVGPTGAGKSDLAVEIALELGGEIVNADSMQLYRGMDIGTAKIPMVERRGVPHHLL
DVWEITRAADVASYQADARRVVNGLLAAGRVPILVGGSGLYVRAVLDDLSFPGTDPAVRARLERELAEVGPGPLHERLTG
LAPTAAAAILPGNGRRIVRALEVVELTGTFEATLPEYRSIYDVVQVGVDRPDLDTRIADRVELMWRAGFVDEVAALAEAG
LRDGRTASRALGYAQVLAVLDGAMDSMDAAKQATTTATRRFARRQRSWFRRDPRISWLEFPDVAAALAEVRRLVGSL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=26.3665594855305, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1790613, Length=308, Percent_Identity=38.6363636363636, Blast_Score=171, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI25144712, Length=199, Percent_Identity=29.6482412060301, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=25.6849315068493, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24649830, Length=186, Percent_Identity=37.0967741935484, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_FRASN (A8L6K0)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505588.1
- GeneID:   5669641
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1228
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   FSP1855:FRANEAN1_1228-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33968; Mature: 33837

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASRDGAGLTNGATRADQAGPVIAVVGPTGAGKSDLAVEIALELGGEIVNADSMQLYRG
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEHHCCCEECCCHHHHHHC
MDIGTAKIPMVERRGVPHHLLDVWEITRAADVASYQADARRVVNGLLAAGRVPILVGGSG
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
LYVRAVLDDLSFPGTDPAVRARLERELAEVGPGPLHERLTGLAPTAAAAILPGNGRRIVR
EEHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHEEECCCCHHHHH
ALEVVELTGTFEATLPEYRSIYDVVQVGVDRPDLDTRIADRVELMWRAGFVDEVAALAEA
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLRDGRTASRALGYAQVLAVLDGAMDSMDAAKQATTTATRRFARRQRSWFRRDPRISWLE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
FPDVAAALAEVRRLVGSL
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TASRDGAGLTNGATRADQAGPVIAVVGPTGAGKSDLAVEIALELGGEIVNADSMQLYRG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEHHCCCEECCCHHHHHHC
MDIGTAKIPMVERRGVPHHLLDVWEITRAADVASYQADARRVVNGLLAAGRVPILVGGSG
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
LYVRAVLDDLSFPGTDPAVRARLERELAEVGPGPLHERLTGLAPTAAAAILPGNGRRIVR
EEHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHEEECCCCHHHHH
ALEVVELTGTFEATLPEYRSIYDVVQVGVDRPDLDTRIADRVELMWRAGFVDEVAALAEA
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLRDGRTASRALGYAQVLAVLDGAMDSMDAAKQATTTATRRFARRQRSWFRRDPRISWLE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
FPDVAAALAEVRRLVGSL
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA