Click here to switch to the map view.

The map label for this gene is hisS [H]

Identifier: 158313046

GI number: 158313046

Start: 1422912

End: 1424291

Strand: Direct

Name: hisS [H]

Synonym: Franean1_1194

Alternate gene names: 158313046

Gene position: 1422912-1424291 (Clockwise)

Preceding gene: 158313045

Following gene: 158313047

Centisome position: 15.84

GC content: 70.43

Gene sequence:

>1380_bases
GTGCGCCTCGCCCAGGCGCGCGCACGCAACAGTGTCGAGAACGAGGAGTTTGTCCCGGCCATGGCCGACGCACCGATAGT
CCGTCCCACCCCGGTCAGCGGGTTCCCCGAGTGGCTTCCCGACGTCCGGATGGTCGAGCTCCGCTGGCTCGACACCATCC
GCGCCACCTTCGAGCGGTACGGGTTCTGCTCCGTGGAGACCCCGTCGGTGGAGGCCCTCGAGGCCCTGATGGCCAAGGGC
GAGACGTCGCAGGAGGTCTACACCCTGCGCCGCCTGCAGGCAGAGGACGACGACGACAGCGCGCGCCTCGGCCTGCACTT
CGACCTGACCGTGCCGTTCGCCCGGTACGTCGCCGCCCACTTCAACGACCTGGTCTTCCCGTTCAAGCGCTACCAGATCC
AGCGGGTGTGGCGGGGCGAGCGGCCGCAGGAGGGCCGGTTCCGGGAGTTCACTCAGTGTGACATCGACGTCATCAACGTC
GACAACGTCCCGCTGCACTTCGACGCCGAGCTGCCCCGGATCGTGCACGAGGTCCTCACGACGCTCGCCGTCCCGTCCTG
GACGTTGAACATCAACAACCGCAAGATCCTGCAGGGGTTCTACGAGGGGCTCGGTATCGGTGACCCGCTCGCCGTCATCC
GCGCCGCGGACAAGATCGACAAGATCGGCGCGCGCGGGGTCGAGGCGCTGCTGACGGGGCCGGTCGGGCTCACGGCCGAG
CAGGCGCGCGCCTGCCTCGACCTGGCCCAGGTCCGGGGCTCCGACGCCGGTGTCATCGACGAGATCGGCCGGCTCGGGGT
CAAGTCCGAGCTCCTGACGGAAGGGCTCGACGAGCTGGCCCGGGTGCTGGACGACCTGGCCGACCTGCCGGCGGGCGCCG
TCGTGGCCGACCTGTCGATCGCCCGCGGCCTGGACTACTACACCGGCACCGTCTACGAGGCCAAATTCGTCGATGCGCCC
GGGTACGGCAGCATCTGCTCCGGTGGCCGGTACGACGACCTCGCGGGCACCTTCATCCGGCGCAACCTCCCCGGTGTCGG
GATCTCGATCGGTCTCACCCGCATCTTCGCCAAGCTTGTCGCGGACGGCCTCATCGGCGCGGGCCCGTCCAGCCCGGCCC
AGGTGCTCATGGTGATCCCGAGCGACGAGCGCCGCGCCGAGGCGCTCGCGACCGCCCGGCTGCTGCGCGGCCGCGGCCTC
AACGTGGAGACCTACCACCAGGCCGACAAGCTGGCGAAGCAGATCAGGTACGCCTCACGCAAGGGCATCGGGCACGTCTG
GTTCCCGCCGTTCGCCGACGACCGCCCGCACGAGGTGAAGGACATGGCCACCGGCGACCAGGGCCCGGCCGACCCGACGG
CGTGGACACCCGCGGGCTGA

Upstream 100 bases:

>100_bases
CGCTCGCCGTCCGGAGGGACCCGCGGGCGGCGTTGTATGGTTTCGATCGTGCTTCTCCTGTCTCGACATCGGTGATCGCC
GCGCTCTCAGGGTCCGATGA

Downstream 100 bases:

>100_bases
CGGCCGACACGGCACCGCACGGCCGACACAGCACCGCACGGCCCCGCGCGAGCACCGCACGGCCCCGCGCGATTCACGGT
AGGCGCCGAGCGCCACGCGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MRLAQARARNSVENEEFVPAMADAPIVRPTPVSGFPEWLPDVRMVELRWLDTIRATFERYGFCSVETPSVEALEALMAKG
ETSQEVYTLRRLQAEDDDDSARLGLHFDLTVPFARYVAAHFNDLVFPFKRYQIQRVWRGERPQEGRFREFTQCDIDVINV
DNVPLHFDAELPRIVHEVLTTLAVPSWTLNINNRKILQGFYEGLGIGDPLAVIRAADKIDKIGARGVEALLTGPVGLTAE
QARACLDLAQVRGSDAGVIDEIGRLGVKSELLTEGLDELARVLDDLADLPAGAVVADLSIARGLDYYTGTVYEAKFVDAP
GYGSICSGGRYDDLAGTFIRRNLPGVGISIGLTRIFAKLVADGLIGAGPSSPAQVLMVIPSDERRAEALATARLLRGRGL
NVETYHQADKLAKQIRYASRKGIGHVWFPPFADDRPHEVKDMATGDQGPADPTAWTPAG

Sequences:

>Translated_459_residues
MRLAQARARNSVENEEFVPAMADAPIVRPTPVSGFPEWLPDVRMVELRWLDTIRATFERYGFCSVETPSVEALEALMAKG
ETSQEVYTLRRLQAEDDDDSARLGLHFDLTVPFARYVAAHFNDLVFPFKRYQIQRVWRGERPQEGRFREFTQCDIDVINV
DNVPLHFDAELPRIVHEVLTTLAVPSWTLNINNRKILQGFYEGLGIGDPLAVIRAADKIDKIGARGVEALLTGPVGLTAE
QARACLDLAQVRGSDAGVIDEIGRLGVKSELLTEGLDELARVLDDLADLPAGAVVADLSIARGLDYYTGTVYEAKFVDAP
GYGSICSGGRYDDLAGTFIRRNLPGVGISIGLTRIFAKLVADGLIGAGPSSPAQVLMVIPSDERRAEALATARLLRGRGL
NVETYHQADKLAKQIRYASRKGIGHVWFPPFADDRPHEVKDMATGDQGPADPTAWTPAG
>Mature_459_residues
MRLAQARARNSVENEEFVPAMADAPIVRPTPVSGFPEWLPDVRMVELRWLDTIRATFERYGFCSVETPSVEALEALMAKG
ETSQEVYTLRRLQAEDDDDSARLGLHFDLTVPFARYVAAHFNDLVFPFKRYQIQRVWRGERPQEGRFREFTQCDIDVINV
DNVPLHFDAELPRIVHEVLTTLAVPSWTLNINNRKILQGFYEGLGIGDPLAVIRAADKIDKIGARGVEALLTGPVGLTAE
QARACLDLAQVRGSDAGVIDEIGRLGVKSELLTEGLDELARVLDDLADLPAGAVVADLSIARGLDYYTGTVYEAKFVDAP
GYGSICSGGRYDDLAGTFIRRNLPGVGISIGLTRIFAKLVADGLIGAGPSSPAQVLMVIPSDERRAEALATARLLRGRGL
NVETYHQADKLAKQIRYASRKGIGHVWFPPFADDRPHEVKDMATGDQGPADPTAWTPAG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=428, Percent_Identity=33.8785046728972, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI15029520, Length=443, Percent_Identity=31.8284424379233, Blast_Score=178, Evalue=8e-45,
Organism=Escherichia coli, GI1788861, Length=447, Percent_Identity=25.2796420581655, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71993693, Length=405, Percent_Identity=31.8518518518519, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI71993686, Length=405, Percent_Identity=31.8518518518519, Blast_Score=160, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6325290, Length=385, Percent_Identity=31.4285714285714, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24643061, Length=427, Percent_Identity=30.9133489461358, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24643059, Length=426, Percent_Identity=30.0469483568075, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50350; Mature: 50350

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAQARARNSVENEEFVPAMADAPIVRPTPVSGFPEWLPDVRMVELRWLDTIRATFERY
CCCCHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHC
GFCSVETPSVEALEALMAKGETSQEVYTLRRLQAEDDDDSARLGLHFDLTVPFARYVAAH
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHHHHHHHH
FNDLVFPFKRYQIQRVWRGERPQEGRFREFTQCDIDVINVDNVPLHFDAELPRIVHEVLT
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEECCCCCEEECCHHHHHHHHHHH
TLAVPSWTLNINNRKILQGFYEGLGIGDPLAVIRAADKIDKIGARGVEALLTGPVGLTAE
HHCCCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QARACLDLAQVRGSDAGVIDEIGRLGVKSELLTEGLDELARVLDDLADLPAGAVVADLSI
HHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ARGLDYYTGTVYEAKFVDAPGYGSICSGGRYDDLAGTFIRRNLPGVGISIGLTRIFAKLV
HHCCCHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ADGLIGAGPSSPAQVLMVIPSDERRAEALATARLLRGRGLNVETYHQADKLAKQIRYASR
HHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KGIGHVWFPPFADDRPHEVKDMATGDQGPADPTAWTPAG
CCCCEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLAQARARNSVENEEFVPAMADAPIVRPTPVSGFPEWLPDVRMVELRWLDTIRATFERY
CCCCHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHC
GFCSVETPSVEALEALMAKGETSQEVYTLRRLQAEDDDDSARLGLHFDLTVPFARYVAAH
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHHHHHHHH
FNDLVFPFKRYQIQRVWRGERPQEGRFREFTQCDIDVINVDNVPLHFDAELPRIVHEVLT
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCEEEEECCCCCEEECCHHHHHHHHHHH
TLAVPSWTLNINNRKILQGFYEGLGIGDPLAVIRAADKIDKIGARGVEALLTGPVGLTAE
HHCCCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QARACLDLAQVRGSDAGVIDEIGRLGVKSELLTEGLDELARVLDDLADLPAGAVVADLSI
HHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ARGLDYYTGTVYEAKFVDAPGYGSICSGGRYDDLAGTFIRRNLPGVGISIGLTRIFAKLV
HHCCCHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ADGLIGAGPSSPAQVLMVIPSDERRAEALATARLLRGRGLNVETYHQADKLAKQIRYASR
HHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KGIGHVWFPPFADDRPHEVKDMATGDQGPADPTAWTPAG
CCCCEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA