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The map label for this gene is leuA

Identifier: 158312949

GI number: 158312949

Start: 1307791

End: 1309488

Strand: Direct

Name: leuA

Synonym: Franean1_1095

Alternate gene names: 158312949

Gene position: 1307791-1309488 (Clockwise)

Preceding gene: 158312948

Following gene: 158312952

Centisome position: 14.56

GC content: 69.73

Gene sequence:

>1698_bases
GTGATCACACCGCAGCAACCGTCCGGCATGCCCATTGAGAAGTACCGCCCGTTCCCGGCGGTCGCGCTGCCGGACCGCAC
CTGGCCCAACGCCACGATCACCGAGGCCCCGCGCTGGTGCAGCGTCGACCTGCGGGACGGCAACCAGGCCCTGATCGACC
CGATGACGCCGTCGCGCAAGATGCAGCTGTTCGAGCTGCTGGTCGCGATGGGCTACAAGGAGATCGAGGTCGGCTTCCCG
GCGGCGAGCCAGACCGACTTCGACTTCGTCCGTCAGCTGATCGAGGATGACCTGATCCCCGACGACGTGACGATCCAGGT
GCTGACCCAGGCCCGCGAGGAGCTGATCGAGCGCACCGTCACCTCGCTGGTCGGTTCCGACCGCGCTCTGATCCATCTGT
ACAACTCGACCTCCACCCTGCAGCGTCGCGTCGTGTTCGGCCTGGACCGGGCCGGCGTCACCGACATCGCGGTGCAGGGT
GCCCGCTGGTGCGCCCAGTACGCGGAGAAGCTGCTGGAGGGGACCGACGTCCGCTGGCAGTACAGCCCCGAGTCGTTCAC
CGGTACGGAGCTGGACTACGCCCTGGAGGTCTGCGAGGCGGTGATGGACGTCTGGGCGCCGACCCCGGACAACCCCGTCG
TGCTGAACCTGCCCGCCACGGTCGAGATGTCGACGCCGAACGTGTACGCCGACCAGATCGAGTGGATGAGCCGCAACCTG
ACCCGCCGGGACGCGGTGGTGCTGTCGCTGCACCCGCACAACGACCGGGGCTGCGCGGTCGCCGCGGCGGAGCTGGGCGT
CATGGCCGGCGCCGACCGGGTGGAGGGCTGCCTGTTCGGTAACGGCGAGCGTACCGGCAACGTCTGCCTGGTCACGCTGG
GGATGAACCTGTTCTCCCAGGGCATCGATCCGATGATCGACCTCTCCGACATCGACGGCGTGCGCCGCACCGTGGAGTAC
TGCAACCAGCTGCCCGTGCACCCGCGCCATCCCTACGGCGGCGACCTTGTCTACACGGCCTTCTCCGGCTCCCACCAGGA
CGCCATCAAGAAGGGCTTCGAGGCGATGGAGCGGACCGGCCAGACGCTGTGGGAGGTCCCCTACCTGCCGATCGACCCGA
AGGACGTCGGCCGCTCGTACGAGGCCGTCATCCGGGTGAACAGCCAGTCGGGCAAGGGCGGCGTGGCGTACCTGATGAAG
TCGGAGTACGCGCTGGACCTGCCCCGCCGGCTGCAGATCGAGTTCTCGGGCGTGGTCCAGAAGCACACCGACGACGCCGG
CGGCGAGGTCACGGCCGGCCAGCTCTGGGAGATCTTCACCCGGGAGTACCGGCCGGAGGGCGGGGACCCGGCGGCATCGC
TGGAACTGCTCGGATCACGGACGACCGCGGCGAGCGGCGCACGGGACGAGGTGACCGTCTCGGTACGCCTGGACGGCACG
GAGACGGTCATCACGGGCAGCGGCAACGGCCCGATCGACGCGTTCGTGGCCGCGCTGTCCGAGCGGGGTGTGCCGGTGCG
CGTCCTCGACTACAACGAGCACGCGCTGGGTTCGGGCGCGGACGCCCGTGCCGCGGCCTACCTGGAGGTCGCGGTGGGGG
AGACGGTGCTGTGGGGGGTCGGGATCGACCCCAACATCGTCACCGCCTCGCTGCGCGCGGTCGTCAGCGCCGTGAACCGG
GCGTCCCGGGAGAGCTGA

Upstream 100 bases:

>100_bases
GGAGAGATGGCTGGCTTCCCCGCCGCGGAGTTCTCGTGCGTCAGCGCTGGGTCCCGCTCGGCGGGGGATAACCGCCGGCT
CTTCCGAGGAAGCAGGAGCA

Downstream 100 bases:

>100_bases
GTATCCGGCCGGTGCGGGTGGGCGGGATGCCGCGCCGTCGTCCGCACCGGCCACGACCTGCGCCGCGGCCAGTGCCGTGG
TCAGTCGTTGAGGGAGGCCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MITPQQPSGMPIEKYRPFPAVALPDRTWPNATITEAPRWCSVDLRDGNQALIDPMTPSRKMQLFELLVAMGYKEIEVGFP
AASQTDFDFVRQLIEDDLIPDDVTIQVLTQAREELIERTVTSLVGSDRALIHLYNSTSTLQRRVVFGLDRAGVTDIAVQG
ARWCAQYAEKLLEGTDVRWQYSPESFTGTELDYALEVCEAVMDVWAPTPDNPVVLNLPATVEMSTPNVYADQIEWMSRNL
TRRDAVVLSLHPHNDRGCAVAAAELGVMAGADRVEGCLFGNGERTGNVCLVTLGMNLFSQGIDPMIDLSDIDGVRRTVEY
CNQLPVHPRHPYGGDLVYTAFSGSHQDAIKKGFEAMERTGQTLWEVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYLMK
SEYALDLPRRLQIEFSGVVQKHTDDAGGEVTAGQLWEIFTREYRPEGGDPAASLELLGSRTTAASGARDEVTVSVRLDGT
ETVITGSGNGPIDAFVAALSERGVPVRVLDYNEHALGSGADARAAAYLEVAVGETVLWGVGIDPNIVTASLRAVVSAVNR
ASRES

Sequences:

>Translated_565_residues
MITPQQPSGMPIEKYRPFPAVALPDRTWPNATITEAPRWCSVDLRDGNQALIDPMTPSRKMQLFELLVAMGYKEIEVGFP
AASQTDFDFVRQLIEDDLIPDDVTIQVLTQAREELIERTVTSLVGSDRALIHLYNSTSTLQRRVVFGLDRAGVTDIAVQG
ARWCAQYAEKLLEGTDVRWQYSPESFTGTELDYALEVCEAVMDVWAPTPDNPVVLNLPATVEMSTPNVYADQIEWMSRNL
TRRDAVVLSLHPHNDRGCAVAAAELGVMAGADRVEGCLFGNGERTGNVCLVTLGMNLFSQGIDPMIDLSDIDGVRRTVEY
CNQLPVHPRHPYGGDLVYTAFSGSHQDAIKKGFEAMERTGQTLWEVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYLMK
SEYALDLPRRLQIEFSGVVQKHTDDAGGEVTAGQLWEIFTREYRPEGGDPAASLELLGSRTTAASGARDEVTVSVRLDGT
ETVITGSGNGPIDAFVAALSERGVPVRVLDYNEHALGSGADARAAAYLEVAVGETVLWGVGIDPNIVTASLRAVVSAVNR
ASRES
>Mature_565_residues
MITPQQPSGMPIEKYRPFPAVALPDRTWPNATITEAPRWCSVDLRDGNQALIDPMTPSRKMQLFELLVAMGYKEIEVGFP
AASQTDFDFVRQLIEDDLIPDDVTIQVLTQAREELIERTVTSLVGSDRALIHLYNSTSTLQRRVVFGLDRAGVTDIAVQG
ARWCAQYAEKLLEGTDVRWQYSPESFTGTELDYALEVCEAVMDVWAPTPDNPVVLNLPATVEMSTPNVYADQIEWMSRNL
TRRDAVVLSLHPHNDRGCAVAAAELGVMAGADRVEGCLFGNGERTGNVCLVTLGMNLFSQGIDPMIDLSDIDGVRRTVEY
CNQLPVHPRHPYGGDLVYTAFSGSHQDAIKKGFEAMERTGQTLWEVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYLMK
SEYALDLPRRLQIEFSGVVQKHTDDAGGEVTAGQLWEIFTREYRPEGGDPAASLELLGSRTTAASGARDEVTVSVRLDGT
ETVITGSGNGPIDAFVAALSERGVPVRVLDYNEHALGSGADARAAAYLEVAVGETVLWGVGIDPNIVTASLRAVVSAVNR
ASRES

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=529, Percent_Identity=25.1417769376182, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=561, Percent_Identity=48.6631016042781, Blast_Score=555, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6324682, Length=562, Percent_Identity=47.3309608540925, Blast_Score=544, Evalue=1e-155,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_FRASN (A8L551)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505457.1
- ProteinModelPortal:   A8L551
- SMR:   A8L551
- GeneID:   5669509
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1095
- HOGENOM:   HBG289752
- OMA:   APRWCAV
- ProtClustDB:   PRK03739
- BioCyc:   FSP1855:FRANEAN1_1095-MONOMER
- HAMAP:   MF_00572
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR10277:SF2
- SMART:   SM00917
- TIGRFAMs:   TIGR00970

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 61635; Mature: 61635

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITPQQPSGMPIEKYRPFPAVALPDRTWPNATITEAPRWCSVDLRDGNQALIDPMTPSRK
CCCCCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHH
MQLFELLVAMGYKEIEVGFPAASQTDFDFVRQLIEDDLIPDDVTIQVLTQAREELIERTV
HHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TSLVGSDRALIHLYNSTSTLQRRVVFGLDRAGVTDIAVQGARWCAQYAEKLLEGTDVRWQ
HHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
YSPESFTGTELDYALEVCEAVMDVWAPTPDNPVVLNLPATVEMSTPNVYADQIEWMSRNL
ECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHCCC
TRRDAVVLSLHPHNDRGCAVAAAELGVMAGADRVEGCLFGNGERTGNVCLVTLGMNLFSQ
CCCCEEEEEECCCCCCCCEEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEECHHHHHC
GIDPMIDLSDIDGVRRTVEYCNQLPVHPRHPYGGDLVYTAFSGSHQDAIKKGFEAMERTG
CCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
QTLWEVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYLMKSEYALDLPRRLQIEFSGVVQ
CHHHCCCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEECHHE
KHTDDAGGEVTAGQLWEIFTREYRPEGGDPAASLELLGSRTTAASGARDEVTVSVRLDGT
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCC
ETVITGSGNGPIDAFVAALSERGVPVRVLDYNEHALGSGADARAAAYLEVAVGETVLWGV
EEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHEEEEEEEECCEEEEEC
GIDPNIVTASLRAVVSAVNRASRES
CCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITPQQPSGMPIEKYRPFPAVALPDRTWPNATITEAPRWCSVDLRDGNQALIDPMTPSRK
CCCCCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHH
MQLFELLVAMGYKEIEVGFPAASQTDFDFVRQLIEDDLIPDDVTIQVLTQAREELIERTV
HHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TSLVGSDRALIHLYNSTSTLQRRVVFGLDRAGVTDIAVQGARWCAQYAEKLLEGTDVRWQ
HHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
YSPESFTGTELDYALEVCEAVMDVWAPTPDNPVVLNLPATVEMSTPNVYADQIEWMSRNL
ECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHCCC
TRRDAVVLSLHPHNDRGCAVAAAELGVMAGADRVEGCLFGNGERTGNVCLVTLGMNLFSQ
CCCCEEEEEECCCCCCCCEEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEECHHHHHC
GIDPMIDLSDIDGVRRTVEYCNQLPVHPRHPYGGDLVYTAFSGSHQDAIKKGFEAMERTG
CCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
QTLWEVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYLMKSEYALDLPRRLQIEFSGVVQ
CHHHCCCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEECHHE
KHTDDAGGEVTAGQLWEIFTREYRPEGGDPAASLELLGSRTTAASGARDEVTVSVRLDGT
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCC
ETVITGSGNGPIDAFVAALSERGVPVRVLDYNEHALGSGADARAAAYLEVAVGETVLWGV
EEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHEEEEEEEECCEEEEEC
GIDPNIVTASLRAVVSAVNRASRES
CCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA