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The map label for this gene is leuB

Identifier: 158312952

GI number: 158312952

Start: 1311667

End: 1312695

Strand: Direct

Name: leuB

Synonym: Franean1_1098

Alternate gene names: 158312952

Gene position: 1311667-1312695 (Clockwise)

Preceding gene: 158312949

Following gene: 158312953

Centisome position: 14.6

GC content: 74.25

Gene sequence:

>1029_bases
ATGAGGCTTGCGGTCATTGGTGGCGACGGAATCGGCCCGGAAGTGGTCGCGGAGGGGCTGCGCGTGCTACGCGCCGTGCA
TCCCAAAGTGGAGACCACCGACTACGACCTGGGCGCGCGGCGCTGGCACGAGACCGGCGAGACCCTGCCCGACAGCGTCC
TGGCGGAGCTGCGCGGGCACGACGCGATCCTGCTCGGCGCTGTCGGCGACCCCGGGGTACCCAGCGGCGTCCTGGAACGC
GGGCTGCTGCTGCGGCTGCGGTTCGAGCTGGACCACCACGTCAACCTCCGGCCGGTCCGGCTGTACCCCGGAGTGACCTC
ACCGCTCGCCGGTGACCCCGCCATCGACATGATCGTGGTGCGGGAGGGGACGGAGGGCCCCTACGCCGGCGCCGGCGGCA
CTCTGCGGCGCGGGACGCCGCAGGAGGTGGCGACCGAGGAGAGCCTGAACACGCGCTTCGGGGTCGAGCGGGTCGTGCGC
GACGCCTTCGCGCGGGCGAGCCGGCGTCCCCGCGCCCACCTCACCCTGGTACACAAGACCAACGTGCTCACCAAGGCGGG
CGACCTGTGGGCCCGCACGGTCGCCGAGGTCGGCGCCGAGTTCCCCGCCGTCAGCGTCGACTACCAGCACGTGGACGCCG
CGTCGATGTTCTTCGTGACCGACCCGGCCCGGTTCGACGTGGTGGTCACCGACAACATGTTCGGCGACATCCTGACCGAC
ATCGGCGCCGCGATCACCGGCGGCATCGGGCTCGCCGCCAGCGGGAACCTCGACCCCTCGGGCGCGAACCCGAGCATGTT
CGAGCCGGTCCACGGCAGCGCTCCCGACATCGCCGGCCAGGGGCTGGCGGACCCGACCGCGACGGTCGCCTCGGTCGCGA
TGCTGCTGGACCACCTCGGCCACGCCGACGAGGCGGCCCGGGTGGAGGGCGCGGTGGCCGCGTCGCTGGCCGCCCGCGCC
GCGGCCGGCGGTGCCCGCCGCTCCACCCGCGAGATCGGCGACGACCTGGCCACCCGCGCCGCGGGCTGA

Upstream 100 bases:

>100_bases
TCCCCGGGGCGGCCCGGGGCGGCCCGGCGCCCGTCCGGCCTGGTCGCGCCCGGGGCGGGCGCGCCGGACGTCCACGCCGG
GCGGACTAAAGTCGAGCCCC

Downstream 100 bases:

>100_bases
GCCGGGCCCCACTCCGACCGGCCGCGCGAGCGGGAACGATCGCGCGGCCGGCGAGCCGGGGGAGAGCCCGGTGTGTCCCC
GCCGATTCGTCGTTCGTTTT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRLAVIGGDGIGPEVVAEGLRVLRAVHPKVETTDYDLGARRWHETGETLPDSVLAELRGHDAILLGAVGDPGVPSGVLER
GLLLRLRFELDHHVNLRPVRLYPGVTSPLAGDPAIDMIVVREGTEGPYAGAGGTLRRGTPQEVATEESLNTRFGVERVVR
DAFARASRRPRAHLTLVHKTNVLTKAGDLWARTVAEVGAEFPAVSVDYQHVDAASMFFVTDPARFDVVVTDNMFGDILTD
IGAAITGGIGLAASGNLDPSGANPSMFEPVHGSAPDIAGQGLADPTATVASVAMLLDHLGHADEAARVEGAVAASLAARA
AAGGARRSTREIGDDLATRAAG

Sequences:

>Translated_342_residues
MRLAVIGGDGIGPEVVAEGLRVLRAVHPKVETTDYDLGARRWHETGETLPDSVLAELRGHDAILLGAVGDPGVPSGVLER
GLLLRLRFELDHHVNLRPVRLYPGVTSPLAGDPAIDMIVVREGTEGPYAGAGGTLRRGTPQEVATEESLNTRFGVERVVR
DAFARASRRPRAHLTLVHKTNVLTKAGDLWARTVAEVGAEFPAVSVDYQHVDAASMFFVTDPARFDVVVTDNMFGDILTD
IGAAITGGIGLAASGNLDPSGANPSMFEPVHGSAPDIAGQGLADPTATVASVAMLLDHLGHADEAARVEGAVAASLAARA
AAGGARRSTREIGDDLATRAAG
>Mature_342_residues
MRLAVIGGDGIGPEVVAEGLRVLRAVHPKVETTDYDLGARRWHETGETLPDSVLAELRGHDAILLGAVGDPGVPSGVLER
GLLLRLRFELDHHVNLRPVRLYPGVTSPLAGDPAIDMIVVREGTEGPYAGAGGTLRRGTPQEVATEESLNTRFGVERVVR
DAFARASRRPRAHLTLVHKTNVLTKAGDLWARTVAEVGAEFPAVSVDYQHVDAASMFFVTDPARFDVVVTDNMFGDILTD
IGAAITGGIGLAASGNLDPSGANPSMFEPVHGSAPDIAGQGLADPTATVASVAMLLDHLGHADEAARVEGAVAASLAARA
AAGGARRSTREIGDDLATRAAG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=314, Percent_Identity=31.8471337579618, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4758582, Length=315, Percent_Identity=28.8888888888889, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI28178838, Length=315, Percent_Identity=28.8888888888889, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI28178816, Length=300, Percent_Identity=27.3333333333333, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178821, Length=300, Percent_Identity=27.3333333333333, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178819, Length=152, Percent_Identity=29.6052631578947, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788101, Length=327, Percent_Identity=46.7889908256881, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI87081683, Length=359, Percent_Identity=38.9972144846797, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1787381, Length=393, Percent_Identity=29.7709923664122, Blast_Score=117, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71986051, Length=323, Percent_Identity=31.8885448916409, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17550882, Length=306, Percent_Identity=27.7777777777778, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=334, Percent_Identity=29.3413173652695, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=318, Percent_Identity=28.3018867924528, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322097, Length=361, Percent_Identity=33.2409972299169, Blast_Score=165, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=321, Percent_Identity=32.7102803738318, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=34.6368715083799, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=32.6470588235294, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=31.6715542521994, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=31.6715542521994, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24661184, Length=320, Percent_Identity=32.1875, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI161078635, Length=346, Percent_Identity=25.7225433526012, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078639, Length=341, Percent_Identity=26.099706744868, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078633, Length=346, Percent_Identity=25.7225433526012, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24650122, Length=346, Percent_Identity=25.7225433526012, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078637, Length=346, Percent_Identity=25.7225433526012, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281362242, Length=320, Percent_Identity=27.5, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648872, Length=320, Percent_Identity=27.5, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130355, Length=312, Percent_Identity=23.0769230769231, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEU3_FRASN (A8L554)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505460.1
- ProteinModelPortal:   A8L554
- SMR:   A8L554
- GeneID:   5669512
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1098
- HOGENOM:   HBG518924
- OMA:   AATIFMV
- ProtClustDB:   PRK03437
- BioCyc:   FSP1855:FRANEAN1_1098-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01035
- InterPro:   IPR019818
- InterPro:   IPR001804
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 35680; Mature: 35680

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 87-87 BINDING 97-97 BINDING 121-121 BINDING 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAVIGGDGIGPEVVAEGLRVLRAVHPKVETTDYDLGARRWHETGETLPDSVLAELRGH
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCC
DAILLGAVGDPGVPSGVLERGLLLRLRFELDHHVNLRPVRLYPGVTSPLAGDPAIDMIVV
CEEEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEEE
REGTEGPYAGAGGTLRRGTPQEVATEESLNTRFGVERVVRDAFARASRRPRAHLTLVHKT
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
NVLTKAGDLWARTVAEVGAEFPAVSVDYQHVDAASMFFVTDPARFDVVVTDNMFGDILTD
CCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCEEEEEEECCHHHHHHHH
IGAAITGGIGLAASGNLDPSGANPSMFEPVHGSAPDIAGQGLADPTATVASVAMLLDHLG
HHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
HADEAARVEGAVAASLAARAAAGGARRSTREIGDDLATRAAG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLAVIGGDGIGPEVVAEGLRVLRAVHPKVETTDYDLGARRWHETGETLPDSVLAELRGH
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCC
DAILLGAVGDPGVPSGVLERGLLLRLRFELDHHVNLRPVRLYPGVTSPLAGDPAIDMIVV
CEEEEECCCCCCCCHHHHHCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEEE
REGTEGPYAGAGGTLRRGTPQEVATEESLNTRFGVERVVRDAFARASRRPRAHLTLVHKT
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
NVLTKAGDLWARTVAEVGAEFPAVSVDYQHVDAASMFFVTDPARFDVVVTDNMFGDILTD
CCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCEEEEEEECCHHHHHHHH
IGAAITGGIGLAASGNLDPSGANPSMFEPVHGSAPDIAGQGLADPTATVASVAMLLDHLG
HHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
HADEAARVEGAVAASLAARAAAGGARRSTREIGDDLATRAAG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA