Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 158312936

GI number: 158312936

Start: 1288647

End: 1290758

Strand: Direct

Name: ligA

Synonym: Franean1_1082

Alternate gene names: 158312936

Gene position: 1288647-1290758 (Clockwise)

Preceding gene: 158312935

Following gene: 158312937

Centisome position: 14.35

GC content: 72.92

Gene sequence:

>2112_bases
GTGAGCGTGGACGAGCGCGGCCTGGCTGCCGACGAGCGCGGCCTGGCCGCTGACGAGGTTGGTCTGGCCGCCGACGAGCG
TGGCCGGGCCGCCGAGCTGGCCCGCGAGCTGGAGGAACACGCCTACCGCTACTACGTGCTCGACAAGCCGACCGTCGCCG
ATGCCGAGTACGACGCGCTTATGCGCGAGCTGGAGGCGATCGAGGAGCGGCACCCCGAGCTGCGCACCCCGGACTCGCCG
ACCCAGAAGGTCGCAGGCTCCTACTCGACGCTGTTCACCCCGGTCGAGCATCTGGAACGGCTGCTGTCACTCGACAACGT
CTTCACCGACGAGGAGTTCCACGCCTGGGCGGCGCGCGCCGCGCGGGAGCAGCCTGTCACGGCGTGGCTGTGCGAGCTGA
AGATCGACGGGCTGGCGGTCGACCTCGTCTACGAGGGCGGCCGGCTCGTCCGCGCGGCCACCCGCGGCGACGGTCGCACG
GGGGAGGACATCACGCCCAACGTGCGGACCCTGGCGAGCGTGCCGTCCCGGCTGCGCGGCCCGGCCGTGCCGGAGCTGCT
CGAGGTGCGCGGCGAGGTGTTCTTCCCGACCGAGCGGTTCACCGAGCTCAACGCCGCGCTGGTTGAGGCGGGGAAGGCAC
CGTTCGCCAACCCGCGCAACGCCGCCGCCGGGTCGCTGCGCCAGAAGGACCCCCGGGTCACCGCCAGCCGTCCGCTCGAC
ATGATCGTGCACGGCCTCGGCGCCCACCAGGGCTTCGACGTGGCGTCCCAGTCGGGCGCGTACGCGCGGCTCGCCGAGCT
GGGGCTGCCGGTCTCCGCCCGTCACGAGGTGCTGGCCTCGGTGGAGGACGTGCTCGCCTTCGTCCGGCGCTGGGGCGAGC
ACCGCCACGACGTCGAGCACGAGATCGACGGGGTCGTCGTGAAGGTCGACGACTTCGGCCAGCAGCGTCGCCTCGGCGCC
ACCTCGAAGGCCCCCCGCTGGGCGGTCGCGTTCAAGTACCCGCCGGAGGAGGTCACCACCCGGCTGCGCGACATCCAGGT
GAACGTCGGCCGCACCGGCCGGGTGACCCCGTTCGGGGTGCTCGAGCCGGTGAAGGTGGCGGGGTCGACGGTGGCGATGG
CCACCCTCCACAACATCGACGAGGTGGGTCGCAAAGGCGTCCTGATCGGCGACACCGTGGTGCTGCGCAAGGCCGGCGAC
GTCATCCCCGAGATCGTCAGCCCGGTCGTCGACCTGCGTGACGGTTCCGAGCGCGCGTTCGTGATGCCCACGCACTGCCC
GGAGTGCGGCACCCAGCTGGTCCGCCCCGAGGGCGAGGTCGACATCCGCTGCCCGAACACCGTCTCGTGCCCGGCGCAGC
TGCGGGAGTCGGTGTTCCACCTGGCATCGCGCGGCGCGCTCGACATCGACGGGCTCGGTTACGAGACGGCGACGGTCCTG
CTCGCGGAGGGCCGGATCCACGACATCGGCGACGTCTTCCACCTGGCCCCGGAGTCCTTCGAGGGGCTGCGTGGCTTCGC
CGACAAGAAGATCGAGCAGATCCTGCGCGGCCTGGAGGCGGCTCGCGACCGCCCGCTGTGGCGGTTGCTGGTCGGGCTGT
CGATCCGTCACGTCGGGCCGACGGCCGCCCGCGGGCTGGCCCGGGAGCTGCGTTCCCTCGACGCGATCGCGACGGCGCCC
GCCGAGCGGCTCGCGGCCGTCGACGGCGTCGGGCCGAAGATCGCCGACGCGGTGGTCGACTGGTTCGCCGACCCGCGGCA
CCGCGACATCGTCGCCCGGATCGCCTCGGGCGGGGTCCGCCTGGCCGACGAGGGCGCCGGCGAGGGCCCCGGGCCGCTGG
ACGGCGTCGTGGTGGTCATCACCGGCACCCTGGACGGCTGGAGTCGGGACACGGCGACCGAGGCGGTGCAGTCCCGCGGC
GGCAAGGTGACGGGCTCGGTGAGCAAGAAGACGACGTTCGTCGTCGCCGGCGCCGATCCCGGGGCGTCGAAGTACGACAA
GGCCCGCACCCTGAAGATCCCGCTCCTGGACGAAGCCGGATTCGCGGTGCTTCTCCGTGACGGAGCGGATGCGGCCAGGG
CACTCGCCGTGCCCGAGGAGGTCGACGGGTAA

Upstream 100 bases:

>100_bases
TGACACCGACGTGTGGTCTGGCCAATGCCAGGGTGGGTGACGCGCGCGCCGCGCTGCGGCACTGCCGTGAGGCGGCGCGG
GTCCTGGTGGAATCTCCACA

Downstream 100 bases:

>100_bases
CCTTCGCATCCTGGCAGCGAAAGGTTCATTACGGTGAGCACTCATATGTTCCGTCATGTTCTCGTCGGTGTGGCCGGGCG
TCGGTGCGCCCGTCCCCGAC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MSVDERGLAADERGLAADEVGLAADERGRAAELARELEEHAYRYYVLDKPTVADAEYDALMRELEAIEERHPELRTPDSP
TQKVAGSYSTLFTPVEHLERLLSLDNVFTDEEFHAWAARAAREQPVTAWLCELKIDGLAVDLVYEGGRLVRAATRGDGRT
GEDITPNVRTLASVPSRLRGPAVPELLEVRGEVFFPTERFTELNAALVEAGKAPFANPRNAAAGSLRQKDPRVTASRPLD
MIVHGLGAHQGFDVASQSGAYARLAELGLPVSARHEVLASVEDVLAFVRRWGEHRHDVEHEIDGVVVKVDDFGQQRRLGA
TSKAPRWAVAFKYPPEEVTTRLRDIQVNVGRTGRVTPFGVLEPVKVAGSTVAMATLHNIDEVGRKGVLIGDTVVLRKAGD
VIPEIVSPVVDLRDGSERAFVMPTHCPECGTQLVRPEGEVDIRCPNTVSCPAQLRESVFHLASRGALDIDGLGYETATVL
LAEGRIHDIGDVFHLAPESFEGLRGFADKKIEQILRGLEAARDRPLWRLLVGLSIRHVGPTAARGLARELRSLDAIATAP
AERLAAVDGVGPKIADAVVDWFADPRHRDIVARIASGGVRLADEGAGEGPGPLDGVVVVITGTLDGWSRDTATEAVQSRG
GKVTGSVSKKTTFVVAGADPGASKYDKARTLKIPLLDEAGFAVLLRDGADAARALAVPEEVDG

Sequences:

>Translated_703_residues
MSVDERGLAADERGLAADEVGLAADERGRAAELARELEEHAYRYYVLDKPTVADAEYDALMRELEAIEERHPELRTPDSP
TQKVAGSYSTLFTPVEHLERLLSLDNVFTDEEFHAWAARAAREQPVTAWLCELKIDGLAVDLVYEGGRLVRAATRGDGRT
GEDITPNVRTLASVPSRLRGPAVPELLEVRGEVFFPTERFTELNAALVEAGKAPFANPRNAAAGSLRQKDPRVTASRPLD
MIVHGLGAHQGFDVASQSGAYARLAELGLPVSARHEVLASVEDVLAFVRRWGEHRHDVEHEIDGVVVKVDDFGQQRRLGA
TSKAPRWAVAFKYPPEEVTTRLRDIQVNVGRTGRVTPFGVLEPVKVAGSTVAMATLHNIDEVGRKGVLIGDTVVLRKAGD
VIPEIVSPVVDLRDGSERAFVMPTHCPECGTQLVRPEGEVDIRCPNTVSCPAQLRESVFHLASRGALDIDGLGYETATVL
LAEGRIHDIGDVFHLAPESFEGLRGFADKKIEQILRGLEAARDRPLWRLLVGLSIRHVGPTAARGLARELRSLDAIATAP
AERLAAVDGVGPKIADAVVDWFADPRHRDIVARIASGGVRLADEGAGEGPGPLDGVVVVITGTLDGWSRDTATEAVQSRG
GKVTGSVSKKTTFVVAGADPGASKYDKARTLKIPLLDEAGFAVLLRDGADAARALAVPEEVDG
>Mature_702_residues
SVDERGLAADERGLAADEVGLAADERGRAAELARELEEHAYRYYVLDKPTVADAEYDALMRELEAIEERHPELRTPDSPT
QKVAGSYSTLFTPVEHLERLLSLDNVFTDEEFHAWAARAAREQPVTAWLCELKIDGLAVDLVYEGGRLVRAATRGDGRTG
EDITPNVRTLASVPSRLRGPAVPELLEVRGEVFFPTERFTELNAALVEAGKAPFANPRNAAAGSLRQKDPRVTASRPLDM
IVHGLGAHQGFDVASQSGAYARLAELGLPVSARHEVLASVEDVLAFVRRWGEHRHDVEHEIDGVVVKVDDFGQQRRLGAT
SKAPRWAVAFKYPPEEVTTRLRDIQVNVGRTGRVTPFGVLEPVKVAGSTVAMATLHNIDEVGRKGVLIGDTVVLRKAGDV
IPEIVSPVVDLRDGSERAFVMPTHCPECGTQLVRPEGEVDIRCPNTVSCPAQLRESVFHLASRGALDIDGLGYETATVLL
AEGRIHDIGDVFHLAPESFEGLRGFADKKIEQILRGLEAARDRPLWRLLVGLSIRHVGPTAARGLARELRSLDAIATAPA
ERLAAVDGVGPKIADAVVDWFADPRHRDIVARIASGGVRLADEGAGEGPGPLDGVVVVITGTLDGWSRDTATEAVQSRGG
KVTGSVSKKTTFVVAGADPGASKYDKARTLKIPLLDEAGFAVLLRDGADAARALAVPEEVDG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=43.956043956044, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1788750, Length=661, Percent_Identity=45.3857791225416, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI87082305, Length=475, Percent_Identity=24.6315789473684, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_FRASN (A8L538)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505444.1
- ProteinModelPortal:   A8L538
- GeneID:   5669496
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1082
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2516647
- BioCyc:   FSP1855:FRANEAN1_1082-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75717; Mature: 75586

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 134-134 BINDING 132-132 BINDING 155-155 BINDING 192-192 BINDING 308-308 BINDING 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDERGLAADERGLAADEVGLAADERGRAAELARELEEHAYRYYVLDKPTVADAEYDAL
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
MRELEAIEERHPELRTPDSPTQKVAGSYSTLFTPVEHLERLLSLDNVFTDEEFHAWAARA
HHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AREQPVTAWLCELKIDGLAVDLVYEGGRLVRAATRGDGRTGEDITPNVRTLASVPSRLRG
HCCCCCEEEEEEEEECCEEEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
PAVPELLEVRGEVFFPTERFTELNAALVEAGKAPFANPRNAAAGSLRQKDPRVTASRPLD
CCCHHHHHHCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCHH
MIVHGLGAHQGFDVASQSGAYARLAELGLPVSARHEVLASVEDVLAFVRRWGEHRHDVEH
HHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EIDGVVVKVDDFGQQRRLGATSKAPRWAVAFKYPPEEVTTRLRDIQVNVGRTGRVTPFGV
HHCCEEEEECCCCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHEEEECCCCCCCCCCCC
LEPVKVAGSTVAMATLHNIDEVGRKGVLIGDTVVLRKAGDVIPEIVSPVVDLRDGSERAF
CCHHHHCCCHHHHHHHHHHHHHCCCCEEECCCEEEHHHCCHHHHHHHHHHHCCCCCCCEE
VMPTHCPECGTQLVRPEGEVDIRCPNTVSCPAQLRESVFHLASRGALDIDGLGYETATVL
EECCCCCHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCEEEEE
LAEGRIHDIGDVFHLAPESFEGLRGFADKKIEQILRGLEAARDRPLWRLLVGLSIRHVGP
EECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHCCCCC
TAARGLARELRSLDAIATAPAERLAAVDGVGPKIADAVVDWFADPRHRDIVARIASGGVR
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
LADEGAGEGPGPLDGVVVVITGTLDGWSRDTATEAVQSRGGKVTGSVSKKTTFVVAGADP
EECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEEECCCC
GASKYDKARTLKIPLLDEAGFAVLLRDGADAARALAVPEEVDG
CCCCCCCCEEEEEEEECCCCCEEEEECCCCHHHHHCCCHHCCC
>Mature Secondary Structure 
SVDERGLAADERGLAADEVGLAADERGRAAELARELEEHAYRYYVLDKPTVADAEYDAL
CCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
MRELEAIEERHPELRTPDSPTQKVAGSYSTLFTPVEHLERLLSLDNVFTDEEFHAWAARA
HHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AREQPVTAWLCELKIDGLAVDLVYEGGRLVRAATRGDGRTGEDITPNVRTLASVPSRLRG
HCCCCCEEEEEEEEECCEEEEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
PAVPELLEVRGEVFFPTERFTELNAALVEAGKAPFANPRNAAAGSLRQKDPRVTASRPLD
CCCHHHHHHCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCHH
MIVHGLGAHQGFDVASQSGAYARLAELGLPVSARHEVLASVEDVLAFVRRWGEHRHDVEH
HHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EIDGVVVKVDDFGQQRRLGATSKAPRWAVAFKYPPEEVTTRLRDIQVNVGRTGRVTPFGV
HHCCEEEEECCCCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHEEEECCCCCCCCCCCC
LEPVKVAGSTVAMATLHNIDEVGRKGVLIGDTVVLRKAGDVIPEIVSPVVDLRDGSERAF
CCHHHHCCCHHHHHHHHHHHHHCCCCEEECCCEEEHHHCCHHHHHHHHHHHCCCCCCCEE
VMPTHCPECGTQLVRPEGEVDIRCPNTVSCPAQLRESVFHLASRGALDIDGLGYETATVL
EECCCCCHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCEEEEE
LAEGRIHDIGDVFHLAPESFEGLRGFADKKIEQILRGLEAARDRPLWRLLVGLSIRHVGP
EECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHCCCCC
TAARGLARELRSLDAIATAPAERLAAVDGVGPKIADAVVDWFADPRHRDIVARIASGGVR
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
LADEGAGEGPGPLDGVVVVITGTLDGWSRDTATEAVQSRGGKVTGSVSKKTTFVVAGADP
EECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEEECCCC
GASKYDKARTLKIPLLDEAGFAVLLRDGADAARALAVPEEVDG
CCCCCCCCEEEEEEEECCCCCEEEEECCCCHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA