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The map label for this gene is eno

Identifier: 158312663

GI number: 158312663

Start: 945791

End: 947074

Strand: Direct

Name: eno

Synonym: Franean1_0807

Alternate gene names: 158312663

Gene position: 945791-947074 (Clockwise)

Preceding gene: 158312662

Following gene: 158312664

Centisome position: 10.53

GC content: 71.65

Gene sequence:

>1284_bases
GTGCCGTCCATCGAGGCTGTCGGAGCCCGTGAGATCCTCGACTCCCGAGGCAACCCCACCGTCGAGGTCGAGGTCGTCCT
GGAGGACGGCACCCTCGGCCGCGCCGCTGTCCCGAGCGGTGCCAGCACCGGTGCCTTCGAGGCCGTGGAACTGCGCGACG
GCGGTGACCGCTACGGCGGCAAGGGCGTCACGAAGGCCGTTGAGGCGGTCATCGGCCGGATCGGCCCCGCCATCATGGAG
CTGGAGGCCACCGAGCAGCGTCTGCTCGACGCGACCCTGATCGACCTGGACGGCACGCCCGGCAAGTCCGCCCTCGGCGC
GAACGCGCTGCTCGGGGTCAGCCTCGCCGTCGCCAAGGCCGCCGCGGCCTCCAGCGGCCTGCCGCTGTTCCGGTACCTGG
GCGGCCCGAGCGCCCACCTGCTGCCGGTGCCGATGATGAACATCCTCAACGGCGGGGCGCACGCCGACACCAACGTCGAC
ATCCAGGAGTTCATGATCGCGCCGATCGGCGCGGGCACGTTCGCGGAGTCGCTGCGCTGGGGTGCCGAGATCTACCAGGC
GCTCAAGAGCGTGCTCAAGGGCCGTGGCCTGGCCACCGGCGTCGGCGACGAGGGTGGCTTCGCCCCGAGCCTGCCGACGA
ACCGGGAGGCGCTCGACCTGATCGCCGAGGCGATCGACAAGGCCGGCTTCGGGCTCGGCGACGACATCGCGCTGGCGCTC
GACGTCGCCTCCACCGAGTTCTTCGCCGACGGCTCCTACACCTTCGAGGGCAGCGGCCGGTCGGCCGAGCAGATGATCGA
CTACTACGCGGACCTGGTCGACTCCTACCCGATCGTCTCCATCGAGGACCCGCTCGCCGAGGACGACTGGGCCGGCTGGA
CGAGCATCACCACCCGGCTTGGTGCCCGCGTCCAGCTCGTCGGCGACGACCTGTTCGTCACCAACCCGGAGCGGCTGGCC
CGCGGCATCGCCGAGGGGGCGGCGAACGCGCTGCTGGTGAAGGTGAACCAGATCGGCACCCTGACCGAGACCCTGGACGC
GGTGAACCTCGCGCACCGCAGCGGCTACCGCTGCATGATGTCGCACCGCTCCGGCGAGACCGAGGACACCACGATCGCCG
ACCTGGCCGTGGCCGTCGACTGCGGCCAGATCAAGACCGGCGCCCCGGCGCGCAGCGAGCGGGTGGCGAAGTACAACCAG
CTGCTGCGCATCGAGGAGGAGCTGGACGACGCCGCCCGGTACGCGGGAGCCGGCGCGTTCCCGCGGCGGACGACCACCGG
CTGA

Upstream 100 bases:

>100_bases
CCGCCAGAGGTCGTTTCTGTCCGCCAGCAGATGTCTGTCCGCCAGCAGATGCCGTCCCGCGCCGGTGCCCCGGTCGCGTC
TCCATCGAAAGGTCGTGCCC

Downstream 100 bases:

>100_bases
GCCGACCATGCCCGTCCGGCGTACCGCGCTGACGACGAGGGCCACGCTGCTGGCCGTCGTCATCTGCGTCCTGGTGTTGA
CCCTGGCCTATCCGTTGCGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPSIEAVGAREILDSRGNPTVEVEVVLEDGTLGRAAVPSGASTGAFEAVELRDGGDRYGGKGVTKAVEAVIGRIGPAIME
LEATEQRLLDATLIDLDGTPGKSALGANALLGVSLAVAKAAAASSGLPLFRYLGGPSAHLLPVPMMNILNGGAHADTNVD
IQEFMIAPIGAGTFAESLRWGAEIYQALKSVLKGRGLATGVGDEGGFAPSLPTNREALDLIAEAIDKAGFGLGDDIALAL
DVASTEFFADGSYTFEGSGRSAEQMIDYYADLVDSYPIVSIEDPLAEDDWAGWTSITTRLGARVQLVGDDLFVTNPERLA
RGIAEGAANALLVKVNQIGTLTETLDAVNLAHRSGYRCMMSHRSGETEDTTIADLAVAVDCGQIKTGAPARSERVAKYNQ
LLRIEEELDDAARYAGAGAFPRRTTTG

Sequences:

>Translated_427_residues
MPSIEAVGAREILDSRGNPTVEVEVVLEDGTLGRAAVPSGASTGAFEAVELRDGGDRYGGKGVTKAVEAVIGRIGPAIME
LEATEQRLLDATLIDLDGTPGKSALGANALLGVSLAVAKAAAASSGLPLFRYLGGPSAHLLPVPMMNILNGGAHADTNVD
IQEFMIAPIGAGTFAESLRWGAEIYQALKSVLKGRGLATGVGDEGGFAPSLPTNREALDLIAEAIDKAGFGLGDDIALAL
DVASTEFFADGSYTFEGSGRSAEQMIDYYADLVDSYPIVSIEDPLAEDDWAGWTSITTRLGARVQLVGDDLFVTNPERLA
RGIAEGAANALLVKVNQIGTLTETLDAVNLAHRSGYRCMMSHRSGETEDTTIADLAVAVDCGQIKTGAPARSERVAKYNQ
LLRIEEELDDAARYAGAGAFPRRTTTG
>Mature_426_residues
PSIEAVGAREILDSRGNPTVEVEVVLEDGTLGRAAVPSGASTGAFEAVELRDGGDRYGGKGVTKAVEAVIGRIGPAIMEL
EATEQRLLDATLIDLDGTPGKSALGANALLGVSLAVAKAAAASSGLPLFRYLGGPSAHLLPVPMMNILNGGAHADTNVDI
QEFMIAPIGAGTFAESLRWGAEIYQALKSVLKGRGLATGVGDEGGFAPSLPTNREALDLIAEAIDKAGFGLGDDIALALD
VASTEFFADGSYTFEGSGRSAEQMIDYYADLVDSYPIVSIEDPLAEDDWAGWTSITTRLGARVQLVGDDLFVTNPERLAR
GIAEGAANALLVKVNQIGTLTETLDAVNLAHRSGYRCMMSHRSGETEDTTIADLAVAVDCGQIKTGAPARSERVAKYNQL
LRIEEELDDAARYAGAGAFPRRTTTG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=55.011655011655, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=53.3799533799534, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=52.2144522144522, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=52.2144522144522, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=48.5915492957746, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=26.865671641791, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=26.865671641791, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=26.865671641791, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=57.0754716981132, Blast_Score=455, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=52.4249422632794, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=52.4249422632794, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=51.3227513227513, Blast_Score=194, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=51.8518518518518, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=51.8518518518518, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=52.7777777777778, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=51.3888888888889, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=51.6203703703704, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=51.3953488372093, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=51.3953488372093, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=51.3953488372093, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=51.3953488372093, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=51.3953488372093, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=51.3953488372093, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_FRASN (A8L149)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505171.1
- ProteinModelPortal:   A8L149
- SMR:   A8L149
- GeneID:   5669223
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_0807
- HOGENOM:   HBG726599
- OMA:   GSHADSN
- ProtClustDB:   PRK00077
- BioCyc:   FSP1855:FRANEAN1_0807-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44555; Mature: 44423

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 334-334 BINDING 154-154 BINDING 163-163 BINDING 282-282 BINDING 309-309 BINDING 334-334 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIEAVGAREILDSRGNPTVEVEVVLEDGTLGRAAVPSGASTGAFEAVELRDGGDRYGG
CCCCCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
KGVTKAVEAVIGRIGPAIMELEATEQRLLDATLIDLDGTPGKSALGANALLGVSLAVAKA
CHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AAASSGLPLFRYLGGPSAHLLPVPMMNILNGGAHADTNVDIQEFMIAPIGAGTFAESLRW
HHHCCCCCHHHHCCCCCCCEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GAEIYQALKSVLKGRGLATGVGDEGGFAPSLPTNREALDLIAEAIDKAGFGLGDDIALAL
HHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
DVASTEFFADGSYTFEGSGRSAEQMIDYYADLVDSYPIVSIEDPLAEDDWAGWTSITTRL
EECCCEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHH
GARVQLVGDDLFVTNPERLARGIAEGAANALLVKVNQIGTLTETLDAVNLAHRSGYRCMM
CCEEEEECCCEEEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEE
SHRSGETEDTTIADLAVAVDCGQIKTGAPARSERVAKYNQLLRIEEELDDAARYAGAGAF
CCCCCCCCCCCHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRRTTTG
CCCCCCC
>Mature Secondary Structure 
PSIEAVGAREILDSRGNPTVEVEVVLEDGTLGRAAVPSGASTGAFEAVELRDGGDRYGG
CCCCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
KGVTKAVEAVIGRIGPAIMELEATEQRLLDATLIDLDGTPGKSALGANALLGVSLAVAKA
CHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AAASSGLPLFRYLGGPSAHLLPVPMMNILNGGAHADTNVDIQEFMIAPIGAGTFAESLRW
HHHCCCCCHHHHCCCCCCCEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GAEIYQALKSVLKGRGLATGVGDEGGFAPSLPTNREALDLIAEAIDKAGFGLGDDIALAL
HHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
DVASTEFFADGSYTFEGSGRSAEQMIDYYADLVDSYPIVSIEDPLAEDDWAGWTSITTRL
EECCCEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHH
GARVQLVGDDLFVTNPERLARGIAEGAANALLVKVNQIGTLTETLDAVNLAHRSGYRCMM
CCEEEEECCCEEEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEE
SHRSGETEDTTIADLAVAVDCGQIKTGAPARSERVAKYNQLLRIEEELDDAARYAGAGAF
CCCCCCCCCCCHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRRTTTG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA