Click here to switch to the map view.

The map label for this gene is icaA [H]

Identifier: 158312641

GI number: 158312641

Start: 910849

End: 912057

Strand: Direct

Name: icaA [H]

Synonym: Franean1_0785

Alternate gene names: 158312641

Gene position: 910849-912057 (Clockwise)

Preceding gene: 158312639

Following gene: 158312642

Centisome position: 10.14

GC content: 74.28

Gene sequence:

>1209_bases
ATGACAGGAGCTCGTGCATTCGGGAAGGGCACCGCGATCGCGGTTCTCGGCGCGACCGCCGTGTACGGCCATGTTCTGTA
TCCCGCTTACATCGGGTACCGCAGTCGTGGGCTTGCGCCGTCGGTCCCCGCCGAGCCGGACGTGTGGCCGGGCCTCAGCG
TCGTGGTCTCCGCCTACCGCGAGTCGGCGGTCATCGGCACCAAGCTCGACGAGCTGGCCGGTACGGACTACCCCGGCCCG
ATGGAGATCATCGTCGTCGCCGACGACGCGGAGACGGCCACGGCCGCCCGCCGTCCCGGCGTCCGGGTCCTGTCGTCCGG
GGAGCGGCTCGGCAAGGCCCGCGCGGTCAACCGCGGTGTCGCCGCGGCCAGCCACGACGTCGTGGTCCTCACCGACGCCA
ACGCGGTGCTGGCCCCGCACTCGCTGCGCGCCGCAGCGCGCCACTTCACCGACGAGTCCGTCGGCGCCGTCGCCGGCGAG
AAGCAGGTCGACGACCCGGCCGGCGCCCAGGGCTTCTACTGGACGTTCGAGTCCTGGCTCAAGCAGCGCGAGTCCGCGAC
CGGCGCCACCATCGGCGTGGTCGGCGAGATGCTGGCGTTCCGCCGCAAGGCGTTCCGGCCGCTGCCGAAGGACACCGCGG
TGGACGACGCCTGGCTGGCGCTCGACATCCTCGAAAGTGGCCTGCGGGTGGTCTATGAGCCCGAGGCCTACTCGATCGAG
ACCTCCGCGCCGGACTACGCCGCCGAGTGGGAGCGCCGCACCCGCATCGTCGCCGGCAACCTCGACATGCTCTGGCGGCG
CCGCGCCGCGCTCGTGCCCGGCGCGCTGCCGGTCACCTCGCAGCTGTGGGGGCACCGGCTCGTCCGGTCCTCGTTCGGCC
CGCTGGCCCACGTCGCGCTGGTGGCGATCAGCGTCCCGGCGGCCCGCAACAGCTGGGGCGCCCGGCTGTTCCTGCTCGGC
AACGCCGCCGGTGCGGCCAGCGCCGGCGTCCTGATGCGGGGCGGGACCCCGCCCGGGCCGAGCCGCCTGGTGGCCCAGGT
CTTCTTCCTGCAGGCCGTCGCGCTCGGTGGCGTCCGCCGCTTCCTGGCCCGTGACCGGCCGGCGATCTGGCCGAAGCCGG
ACCGGCAGCCCGTGCCGGCGCAGCCGGCGGCGTCCGAAGCCGACATCGACCCCGCCAGCGCGTCCGGGGCCTCCGTGTCC
GTTGTGTGA

Upstream 100 bases:

>100_bases
GGGAGGGACGGGTTGCGCCCGGCCCGCGTCGGTGATCGCCCCGGTAAGGTGCCGGGTAGCCCATTCCGTCACCTGGTAGA
GGCACCGCTACGGTTGTCGC

Downstream 100 bases:

>100_bases
CTGTCGTCCGTCGTGAGCTGACCGTTCCGAGCGGCGAGCTGAGAGGAACAGACCCGTGCCGGACATCGCACCCGACTCGG
TTGCCGACCAGGCGACACCT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTGARAFGKGTAIAVLGATAVYGHVLYPAYIGYRSRGLAPSVPAEPDVWPGLSVVVSAYRESAVIGTKLDELAGTDYPGP
MEIIVVADDAETATAARRPGVRVLSSGERLGKARAVNRGVAAASHDVVVLTDANAVLAPHSLRAAARHFTDESVGAVAGE
KQVDDPAGAQGFYWTFESWLKQRESATGATIGVVGEMLAFRRKAFRPLPKDTAVDDAWLALDILESGLRVVYEPEAYSIE
TSAPDYAAEWERRTRIVAGNLDMLWRRRAALVPGALPVTSQLWGHRLVRSSFGPLAHVALVAISVPAARNSWGARLFLLG
NAAGAASAGVLMRGGTPPGPSRLVAQVFFLQAVALGGVRRFLARDRPAIWPKPDRQPVPAQPAASEADIDPASASGASVS
VV

Sequences:

>Translated_402_residues
MTGARAFGKGTAIAVLGATAVYGHVLYPAYIGYRSRGLAPSVPAEPDVWPGLSVVVSAYRESAVIGTKLDELAGTDYPGP
MEIIVVADDAETATAARRPGVRVLSSGERLGKARAVNRGVAAASHDVVVLTDANAVLAPHSLRAAARHFTDESVGAVAGE
KQVDDPAGAQGFYWTFESWLKQRESATGATIGVVGEMLAFRRKAFRPLPKDTAVDDAWLALDILESGLRVVYEPEAYSIE
TSAPDYAAEWERRTRIVAGNLDMLWRRRAALVPGALPVTSQLWGHRLVRSSFGPLAHVALVAISVPAARNSWGARLFLLG
NAAGAASAGVLMRGGTPPGPSRLVAQVFFLQAVALGGVRRFLARDRPAIWPKPDRQPVPAQPAASEADIDPASASGASVS
VV
>Mature_401_residues
TGARAFGKGTAIAVLGATAVYGHVLYPAYIGYRSRGLAPSVPAEPDVWPGLSVVVSAYRESAVIGTKLDELAGTDYPGPM
EIIVVADDAETATAARRPGVRVLSSGERLGKARAVNRGVAAASHDVVVLTDANAVLAPHSLRAAARHFTDESVGAVAGEK
QVDDPAGAQGFYWTFESWLKQRESATGATIGVVGEMLAFRRKAFRPLPKDTAVDDAWLALDILESGLRVVYEPEAYSIET
SAPDYAAEWERRTRIVAGNLDMLWRRRAALVPGALPVTSQLWGHRLVRSSFGPLAHVALVAISVPAARNSWGARLFLLGN
AAGAASAGVLMRGGTPPGPSRLVAQVFFLQAVALGGVRRFLARDRPAIWPKPDRQPVPAQPAASEADIDPASASGASVSV
V

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 42325; Mature: 42193

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGARAFGKGTAIAVLGATAVYGHVLYPAYIGYRSRGLAPSVPAEPDVWPGLSVVVSAYR
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
ESAVIGTKLDELAGTDYPGPMEIIVVADDAETATAARRPGVRVLSSGERLGKARAVNRGV
HHHHHCCCHHHHCCCCCCCCEEEEEEECCCCHHHHHHCCCCCEECCCHHHHHHHHHHCCC
AAASHDVVVLTDANAVLAPHSLRAAARHFTDESVGAVAGEKQVDDPAGAQGFYWTFESWL
CCCCCCEEEEECCCCEECCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCEEHHHHHHH
KQRESATGATIGVVGEMLAFRRKAFRPLPKDTAVDDAWLALDILESGLRVVYEPEAYSIE
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEE
TSAPDYAAEWERRTRIVAGNLDMLWRRRAALVPGALPVTSQLWGHRLVRSSFGPLAHVAL
CCCCCHHHHHHHCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
VAISVPAARNSWGARLFLLGNAAGAASAGVLMRGGTPPGPSRLVAQVFFLQAVALGGVRR
HHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FLARDRPAIWPKPDRQPVPAQPAASEADIDPASASGASVSVV
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure 
TGARAFGKGTAIAVLGATAVYGHVLYPAYIGYRSRGLAPSVPAEPDVWPGLSVVVSAYR
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
ESAVIGTKLDELAGTDYPGPMEIIVVADDAETATAARRPGVRVLSSGERLGKARAVNRGV
HHHHHCCCHHHHCCCCCCCCEEEEEEECCCCHHHHHHCCCCCEECCCHHHHHHHHHHCCC
AAASHDVVVLTDANAVLAPHSLRAAARHFTDESVGAVAGEKQVDDPAGAQGFYWTFESWL
CCCCCCEEEEECCCCEECCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCEEHHHHHHH
KQRESATGATIGVVGEMLAFRRKAFRPLPKDTAVDDAWLALDILESGLRVVYEPEAYSIE
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEE
TSAPDYAAEWERRTRIVAGNLDMLWRRRAALVPGALPVTSQLWGHRLVRSSFGPLAHVAL
CCCCCHHHHHHHCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
VAISVPAARNSWGARLFLLGNAAGAASAGVLMRGGTPPGPSRLVAQVFFLQAVALGGVRR
HHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FLARDRPAIWPKPDRQPVPAQPAASEADIDPASASGASVSVV
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]