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The map label for this gene is nikB [C]

Identifier: 158312312

GI number: 158312312

Start: 530652

End: 531593

Strand: Direct

Name: nikB [C]

Synonym: Franean1_0450

Alternate gene names: 158312312

Gene position: 530652-531593 (Clockwise)

Preceding gene: 158312311

Following gene: 158312313

Centisome position: 5.91

GC content: 64.54

Gene sequence:

>942_bases
TTGCTAGCCTATGTACTCCGGCGGCTCGGCGCAGGTGTGGTGCTCGCTCTCCTCGTCACATTGATCACGTTCTTCCTGCT
GAGCCTTTCGTTCGACAGCGTGGTCAGGAGCCTCCTTGGCCCCAGCGCGACGCCCGAGACGCTGCATGCACGCAAAGCTC
AGATGGGGCTCGATCGGCCGGTCTTGGTCCAGTATCTCGACTGGCTGGCCCACGCAGCGCGAGGCGACCTCGGCGTCTCG
TACTTCACCAGCGAGCCGGTCGTCCAGGCCGTGCCGACGCGGCTGGGCGTCACCCTGTCCGTCATCGTCGTCGCACTGAT
CCTCACGGCCCTCATCAGTGTCATGCTCGGTGTGCTGGCGGCCAGCCGTGGTGGTGTCGTCGACCGCGTAGCCCAGGGAG
CCTCGCTCGTCGGCCAGCTCGTACCTCACATGCTGATCGCGATCATCCTGGTCACCGTGCTCGCCATCAACCTGAAGCTG
CTCCCCCCCACCGGTTTCACGCATCTCACCGACGACCCGGGCCGCTGGGCGGCCACCATCACCATTCCGGTGATCACCCT
GACGGTGGCCAGTATCGCCAATGCGACGGCGCAGGTCCGCGGCGCCATGGTCGACGAGCTGAGCAAGGACTACATCCGCA
CCCTACGTACCCGCGGAATCTCGTCCCGGTCAATCCTGCTACGCCACGCGCTACGAAACGCGGCGGGTCCCGCGCTCACC
GTGCTGTCCCTGGAATTCATCACGATGTTCGGCGGCGTTCTCATCCTCGAGAACATCTTCGCCCTGCCGGGCTTCGGAAC
CTATGCGTTCAACGCCTCCCTGCAGGGCGACGTCCCGATCATCATGGGAGTCACCCTGTTCGCGGTGCTGCTCATCGTCA
CCGTCAACCTCGTCGTCGACCTCGTGAACGGATGGCTCAACCCGAAGGCGAGAATCCATTGA

Upstream 100 bases:

>100_bases
TCCACTGGGTAACCAAAAAAGGCATCACCTATCTCGGTGACGGCTCGCTGACGTTCAACACCGTTCGCGCCTTCGGCCTG
TCCGGATAACGGAGAACACC

Downstream 100 bases:

>100_bases
CCACGCCGGCATCAGGGACCGACACCACCAAGCGCAGGTCGACGCTGCGCCGACTATGCAAGGACCCGCAGGCAGTCGTC
ACGTCGAGTGTCCTGCTGAT

Product: binding-protein-dependent transport systems inner membrane component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLAYVLRRLGAGVVLALLVTLITFFLLSLSFDSVVRSLLGPSATPETLHARKAQMGLDRPVLVQYLDWLAHAARGDLGVS
YFTSEPVVQAVPTRLGVTLSVIVVALILTALISVMLGVLAASRGGVVDRVAQGASLVGQLVPHMLIAIILVTVLAINLKL
LPPTGFTHLTDDPGRWAATITIPVITLTVASIANATAQVRGAMVDELSKDYIRTLRTRGISSRSILLRHALRNAAGPALT
VLSLEFITMFGGVLILENIFALPGFGTYAFNASLQGDVPIIMGVTLFAVLLIVTVNLVVDLVNGWLNPKARIH

Sequences:

>Translated_313_residues
MLAYVLRRLGAGVVLALLVTLITFFLLSLSFDSVVRSLLGPSATPETLHARKAQMGLDRPVLVQYLDWLAHAARGDLGVS
YFTSEPVVQAVPTRLGVTLSVIVVALILTALISVMLGVLAASRGGVVDRVAQGASLVGQLVPHMLIAIILVTVLAINLKL
LPPTGFTHLTDDPGRWAATITIPVITLTVASIANATAQVRGAMVDELSKDYIRTLRTRGISSRSILLRHALRNAAGPALT
VLSLEFITMFGGVLILENIFALPGFGTYAFNASLQGDVPIIMGVTLFAVLLIVTVNLVVDLVNGWLNPKARIH
>Mature_313_residues
MLAYVLRRLGAGVVLALLVTLITFFLLSLSFDSVVRSLLGPSATPETLHARKAQMGLDRPVLVQYLDWLAHAARGDLGVS
YFTSEPVVQAVPTRLGVTLSVIVVALILTALISVMLGVLAASRGGVVDRVAQGASLVGQLVPHMLIAIILVTVLAINLKL
LPPTGFTHLTDDPGRWAATITIPVITLTVASIANATAQVRGAMVDELSKDYIRTLRTRGISSRSILLRHALRNAAGPALT
VLSLEFITMFGGVLILENIFALPGFGTYAFNASLQGDVPIIMGVTLFAVLLIVTVNLVVDLVNGWLNPKARIH

Specific function: Probably part of the binding-protein-dependent transport system y4tOPQRS for a peptide. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=319, Percent_Identity=34.4827586206897, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1789965, Length=336, Percent_Identity=31.547619047619, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787053, Length=312, Percent_Identity=29.1666666666667, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787497, Length=316, Percent_Identity=29.1139240506329, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788504, Length=289, Percent_Identity=27.3356401384083, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787761, Length=328, Percent_Identity=30.4878048780488, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787550, Length=268, Percent_Identity=26.4925373134328, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33291; Mature: 33291

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAYVLRRLGAGVVLALLVTLITFFLLSLSFDSVVRSLLGPSATPETLHARKAQMGLDRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
VLVQYLDWLAHAARGDLGVSYFTSEPVVQAVPTRLGVTLSVIVVALILTALISVMLGVLA
HHHHHHHHHHHHHCCCCCEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRGGVVDRVAQGASLVGQLVPHMLIAIILVTVLAINLKLLPPTGFTHLTDDPGRWAATI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCEEEEE
TIPVITLTVASIANATAQVRGAMVDELSKDYIRTLRTRGISSRSILLRHALRNAAGPALT
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
VLSLEFITMFGGVLILENIFALPGFGTYAFNASLQGDVPIIMGVTLFAVLLIVTVNLVVD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVNGWLNPKARIH
HHHHCCCCCCCCC
>Mature Secondary Structure
MLAYVLRRLGAGVVLALLVTLITFFLLSLSFDSVVRSLLGPSATPETLHARKAQMGLDRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
VLVQYLDWLAHAARGDLGVSYFTSEPVVQAVPTRLGVTLSVIVVALILTALISVMLGVLA
HHHHHHHHHHHHHCCCCCEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRGGVVDRVAQGASLVGQLVPHMLIAIILVTVLAINLKLLPPTGFTHLTDDPGRWAATI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCEEEEE
TIPVITLTVASIANATAQVRGAMVDELSKDYIRTLRTRGISSRSILLRHALRNAAGPALT
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
VLSLEFITMFGGVLILENIFALPGFGTYAFNASLQGDVPIIMGVTLFAVLLIVTVNLVVD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVNGWLNPKARIH
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424; 8796346 [H]