Click here to switch to the map view.

The map label for this gene is purR [H]

Identifier: 158312299

GI number: 158312299

Start: 517180

End: 518205

Strand: Direct

Name: purR [H]

Synonym: Franean1_0436

Alternate gene names: 158312299

Gene position: 517180-518205 (Clockwise)

Preceding gene: 158312298

Following gene: 158312300

Centisome position: 5.76

GC content: 70.76

Gene sequence:

>1026_bases
GTGGTCTCGACGCCGGACAGCCAGGAGGCGCGCCGCGTCACCATCCAGGACGTGGCACGCGAGGCCGGCGTGTCGGTCTC
GGCCGTCTCCAAGGTCGTCCGGGACGCGTATGGCGTCAGCGAGGGTATGCGGGAGAAGGTCACCGCCGCCATCGACGCGC
TCGGCTACCGCCCGCACACCGGCGCCCGGGCCATGCGCGGCCGCTCGTACTCCGTCGGCGTGATGCTCACCGAGCTGACC
TCGCCGTTCCAGCCGCAGATCATCAACGGCATCACGGCACAGTTCGAGCCGACGCCGTACCAGGAGATCCTGATCGCCGC
CGGCACCTCGCCCGACCGGCAGAAGCGCAGCATCGAGGCCTTGATCGACCGGCAGGTCGACGGGCTGATCGTCATCGCGC
CCTGGATGGAGCAGGCGTGGCTGGAGAAGCTGGGCGCGAGCCTGCCGACGGTCGTGCTGGCCCGGCATGGCGGCTCCGGC
ACCTTCGACACGATCGTCGGCGACGACTTCGAGGGCGCCCGCCTCATGGTCGACCGCCTGGTGGCCCTCGGGCACCGGCG
CATCGTGCACACCAGCCAGCCCTCGGGCGGCCTGGAACGCCCGTACGTCCTGTCGCACACGCCACGGCTCGACGGCTACG
AGGAGACGATGCGAAGGCACGGGCTGGAGCCGGACGTCATCGTCACCAGCTACTCGGAAGAAGGCGGATACGAGGCCGCC
CGGCAAGCGCTGGCCCGTCCCATCCCGCCGACCGCCATCTTCGCCGGCGCGGACATCGCCGCGCTGGGCGTGCTGCGCGC
GGCCGAGGAACTCGGGCTGCGGGTTCCGGAAGACCTCAGCGTCGCCGGGTACGACAATATCTACATGTCGACGATCGGCC
GCATCTCGCTGACCACGATCGACCAGTCGGCCCAGCTCACCGGTTCCCGCAGCGCCCGGTTGCTGCTGGAGCGCATCGAC
GGCCGCACCCAACCGGTGCACTACCTCATCGCGCCGCGCCTGGTGGCTCGCGACACGACCGCCTGA

Upstream 100 bases:

>100_bases
GCTGGCGGGCTCGTTCGCCGTGGTGGGGTCCGCGCGGGTGGTCGAGGGCGCCGAGCGGTCCTTCCTCTCGCGAGCACGCC
CGCACCCATAGGATCGGCGG

Downstream 100 bases:

>100_bases
CACCTCCTTGACGATGGCTTCCCATACCGCCTACGCTGTTTTGTAGAACGTTGCACGGACTGGCCGGCAACGACCCTCCG
GGGGCAGTGCGCAGCCGTTG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MVSTPDSQEARRVTIQDVAREAGVSVSAVSKVVRDAYGVSEGMREKVTAAIDALGYRPHTGARAMRGRSYSVGVMLTELT
SPFQPQIINGITAQFEPTPYQEILIAAGTSPDRQKRSIEALIDRQVDGLIVIAPWMEQAWLEKLGASLPTVVLARHGGSG
TFDTIVGDDFEGARLMVDRLVALGHRRIVHTSQPSGGLERPYVLSHTPRLDGYEETMRRHGLEPDVIVTSYSEEGGYEAA
RQALARPIPPTAIFAGADIAALGVLRAAEELGLRVPEDLSVAGYDNIYMSTIGRISLTTIDQSAQLTGSRSARLLLERID
GRTQPVHYLIAPRLVARDTTA

Sequences:

>Translated_341_residues
MVSTPDSQEARRVTIQDVAREAGVSVSAVSKVVRDAYGVSEGMREKVTAAIDALGYRPHTGARAMRGRSYSVGVMLTELT
SPFQPQIINGITAQFEPTPYQEILIAAGTSPDRQKRSIEALIDRQVDGLIVIAPWMEQAWLEKLGASLPTVVLARHGGSG
TFDTIVGDDFEGARLMVDRLVALGHRRIVHTSQPSGGLERPYVLSHTPRLDGYEETMRRHGLEPDVIVTSYSEEGGYEAA
RQALARPIPPTAIFAGADIAALGVLRAAEELGLRVPEDLSVAGYDNIYMSTIGRISLTTIDQSAQLTGSRSARLLLERID
GRTQPVHYLIAPRLVARDTTA
>Mature_341_residues
MVSTPDSQEARRVTIQDVAREAGVSVSAVSKVVRDAYGVSEGMREKVTAAIDALGYRPHTGARAMRGRSYSVGVMLTELT
SPFQPQIINGITAQFEPTPYQEILIAAGTSPDRQKRSIEALIDRQVDGLIVIAPWMEQAWLEKLGASLPTVVLARHGGSG
TFDTIVGDDFEGARLMVDRLVALGHRRIVHTSQPSGGLERPYVLSHTPRLDGYEETMRRHGLEPDVIVTSYSEEGGYEAA
RQALARPIPPTAIFAGADIAALGVLRAAEELGLRVPEDLSVAGYDNIYMSTIGRISLTTIDQSAQLTGSRSARLLLERID
GRTQPVHYLIAPRLVARDTTA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=321, Percent_Identity=33.6448598130841, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=32.7380952380952, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790369, Length=314, Percent_Identity=31.8471337579618, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787580, Length=335, Percent_Identity=27.1641791044776, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=32.2388059701493, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994940, Length=319, Percent_Identity=29.153605015674, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=27.299703264095, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789202, Length=339, Percent_Identity=29.2035398230088, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790689, Length=328, Percent_Identity=27.4390243902439, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787906, Length=343, Percent_Identity=26.530612244898, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789068, Length=284, Percent_Identity=25.7042253521127, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789456, Length=341, Percent_Identity=24.0469208211144, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=24.2138364779874, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786268, Length=322, Percent_Identity=23.9130434782609, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36968; Mature: 36968

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTPDSQEARRVTIQDVAREAGVSVSAVSKVVRDAYGVSEGMREKVTAAIDALGYRPHT
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GARAMRGRSYSVGVMLTELTSPFQPQIINGITAQFEPTPYQEILIAAGTSPDRQKRSIEA
CHHHHCCCCEEEEEEEECCCCCCCCHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHH
LIDRQVDGLIVIAPWMEQAWLEKLGASLPTVVLARHGGSGTFDTIVGDDFEGARLMVDRL
HHHCCCCCEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
VALGHRRIVHTSQPSGGLERPYVLSHTPRLDGYEETMRRHGLEPDVIVTSYSEEGGYEAA
HHHCCCEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHH
RQALARPIPPTAIFAGADIAALGVLRAAEELGLRVPEDLSVAGYDNIYMSTIGRISLTTI
HHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCEEEEEE
DQSAQLTGSRSARLLLERIDGRTQPVHYLIAPRLVARDTTA
CCCHHCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCC
>Mature Secondary Structure
MVSTPDSQEARRVTIQDVAREAGVSVSAVSKVVRDAYGVSEGMREKVTAAIDALGYRPHT
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GARAMRGRSYSVGVMLTELTSPFQPQIINGITAQFEPTPYQEILIAAGTSPDRQKRSIEA
CHHHHCCCCEEEEEEEECCCCCCCCHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHH
LIDRQVDGLIVIAPWMEQAWLEKLGASLPTVVLARHGGSGTFDTIVGDDFEGARLMVDRL
HHHCCCCCEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
VALGHRRIVHTSQPSGGLERPYVLSHTPRLDGYEETMRRHGLEPDVIVTSYSEEGGYEAA
HHHCCCEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHH
RQALARPIPPTAIFAGADIAALGVLRAAEELGLRVPEDLSVAGYDNIYMSTIGRISLTTI
HHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCEEEEEE
DQSAQLTGSRSARLLLERIDGRTQPVHYLIAPRLVARDTTA
CCCHHCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA