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The map label for this gene is gsiB [H]

Identifier: 158312289

GI number: 158312289

Start: 501353

End: 502888

Strand: Direct

Name: gsiB [H]

Synonym: Franean1_0426

Alternate gene names: 158312289

Gene position: 501353-502888 (Clockwise)

Preceding gene: 158312287

Following gene: 158312290

Centisome position: 5.58

GC content: 65.69

Gene sequence:

>1536_bases
ATGCGACGCAGTAAGTTAGCACTCGTCGCCTCGGCGGTGGCGCTCGCCGCGCCACTCGCCGCGCTGCTGGCCGGGTGCAC
CGGAGAAGCGGCGCCAGGTGCCGAGTCGTCCGCCGGCGCCACCAAGAACACGCTCACGCTCGCCATGTCGGCCGACATCA
CCGGCTGGGACCCCTCGAACCAGCCAGGATACCAGGGCTGGGGACCGGAAGCCATCTGGGACACCCTGATCAAGTGCGAT
GCCTTCGGCAAGCCGGAGGCCGACATCGCCGACAAGTGGACGGTCAGCGCGGACGGCAAGTCCTTCACCGCGCACATCCG
CGAGGGCCAGAAGTTCTCCGACGGCACGCCGGTCGACTCGGCCGCCATCGCGGCATCGTTCCAGTACCTCACCTCGCACG
GCGGGTCCCAGGGCGACTACAAGGGCCTCAAGACCGACGCTCCGGACGCGCAGAACATCACCCTCACCTGGCCCGAGCCG
CAGCCCATGATCATTCAACGGGTCTGCAACCCGAAGATCACCACCAAGGCGCTGCTCGACTCCGGCAAGGTCAACGACAA
GCCGGTCGGATCCGGTCCGTACGTCCTGGACGAAACCGCCACTACCCGCGGCTCGGTGTACACGTTCACCAAGAACGAGA
GCCACTGGAACACTGCCGCTTACCCGTACAAGAAACTGGTTTTGAAGGTCATCGAGAGCGAGACCGCGCGCGTCAGCGCC
CTGAAGACCGGGCAGGTCGACGGCTCGCTGATCACGGCGGCCAGCTACAACGAGGTCGAGGCCTCAGGTCTGAACGTGGT
GACGATGCAGGGCCAGACGACGCGCCTGTTGCTCACCGATCACCTGGGTAAGGAGGTCCCGGCTCTGGGCAGCCTGAAGG
TGCGCCAGGCGATCAACATGGTCTTCGACCGTGACGCCATGGTGAAGAACCTCTACCAGGGTCACGGCAAACCGGCGTAC
CAGATCTTCCGGCCCGGCAGCGACGCGTACATCGACGGCATGGCGGACCCGTACCCCTTCGATGTCACCAAGGCCAAGGC
ACTGATGGCCGAGGCCGGCTACGCCAGCGGCTTCGACCTGAGCCTGCCGACCATGGCCGGTCAGAACCACGAGACCCTCA
TGCCGTACGTGACGCAGCAGCTCGGCCTGCTCGGCATCAAGGTCAAGCAGGTGCCGCTCTCCGGCGCGAACGCGATCGGT
GACCTGCTCAGCGGCACCTACCCGGTGGTCCTGTGGCAGCTCGGCAACTTCGGCCAGTCGCTGCTCGACATCGACGTCGT
GGTGCGCTCGACCGGCTACTGGAACCTCGAGCACCAACCCGACGCGACGGTGGATGGTCTGTGGGAGAAGATCCTCACCG
GCGACGAGGCCACCCGCAAGACCGCCCAGCAGGACATCAACAGGTACGTCATTGAGCAGGCCTGGTTCGCGCCGATGGTC
AACCCCGACGGGTTCTACGCGCACAGCCCGGACGTGAAGATCGATCACGTCTCGGACCACGAGGCGCTGACCCCGAAGCT
GCGCGACTTCAAGTAG

Upstream 100 bases:

>100_bases
CTGTGACGCACGTCCCTACAGTGCCGCGTGGAACGTTGCACAAATCTCCTCCCCTCGAAGCCCGAGTGCCCCGTCTGCGG
CACCCTCGAAGGAGTTCCTC

Downstream 100 bases:

>100_bases
CCGGGAGACCTGCGATGATGCTCACGCTGCTGAAGAACCTCGCGCGCTCGCTGGCCGTGCTGCTGGTGGTCACCTTCGCC
ACGTTCACGCTGATGTTCGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MRRSKLALVASAVALAAPLAALLAGCTGEAAPGAESSAGATKNTLTLAMSADITGWDPSNQPGYQGWGPEAIWDTLIKCD
AFGKPEADIADKWTVSADGKSFTAHIREGQKFSDGTPVDSAAIAASFQYLTSHGGSQGDYKGLKTDAPDAQNITLTWPEP
QPMIIQRVCNPKITTKALLDSGKVNDKPVGSGPYVLDETATTRGSVYTFTKNESHWNTAAYPYKKLVLKVIESETARVSA
LKTGQVDGSLITAASYNEVEASGLNVVTMQGQTTRLLLTDHLGKEVPALGSLKVRQAINMVFDRDAMVKNLYQGHGKPAY
QIFRPGSDAYIDGMADPYPFDVTKAKALMAEAGYASGFDLSLPTMAGQNHETLMPYVTQQLGLLGIKVKQVPLSGANAIG
DLLSGTYPVVLWQLGNFGQSLLDIDVVVRSTGYWNLEHQPDATVDGLWEKILTGDEATRKTAQQDINRYVIEQAWFAPMV
NPDGFYAHSPDVKIDHVSDHEALTPKLRDFK

Sequences:

>Translated_511_residues
MRRSKLALVASAVALAAPLAALLAGCTGEAAPGAESSAGATKNTLTLAMSADITGWDPSNQPGYQGWGPEAIWDTLIKCD
AFGKPEADIADKWTVSADGKSFTAHIREGQKFSDGTPVDSAAIAASFQYLTSHGGSQGDYKGLKTDAPDAQNITLTWPEP
QPMIIQRVCNPKITTKALLDSGKVNDKPVGSGPYVLDETATTRGSVYTFTKNESHWNTAAYPYKKLVLKVIESETARVSA
LKTGQVDGSLITAASYNEVEASGLNVVTMQGQTTRLLLTDHLGKEVPALGSLKVRQAINMVFDRDAMVKNLYQGHGKPAY
QIFRPGSDAYIDGMADPYPFDVTKAKALMAEAGYASGFDLSLPTMAGQNHETLMPYVTQQLGLLGIKVKQVPLSGANAIG
DLLSGTYPVVLWQLGNFGQSLLDIDVVVRSTGYWNLEHQPDATVDGLWEKILTGDEATRKTAQQDINRYVIEQAWFAPMV
NPDGFYAHSPDVKIDHVSDHEALTPKLRDFK
>Mature_511_residues
MRRSKLALVASAVALAAPLAALLAGCTGEAAPGAESSAGATKNTLTLAMSADITGWDPSNQPGYQGWGPEAIWDTLIKCD
AFGKPEADIADKWTVSADGKSFTAHIREGQKFSDGTPVDSAAIAASFQYLTSHGGSQGDYKGLKTDAPDAQNITLTWPEP
QPMIIQRVCNPKITTKALLDSGKVNDKPVGSGPYVLDETATTRGSVYTFTKNESHWNTAAYPYKKLVLKVIESETARVSA
LKTGQVDGSLITAASYNEVEASGLNVVTMQGQTTRLLLTDHLGKEVPALGSLKVRQAINMVFDRDAMVKNLYQGHGKPAY
QIFRPGSDAYIDGMADPYPFDVTKAKALMAEAGYASGFDLSLPTMAGQNHETLMPYVTQQLGLLGIKVKQVPLSGANAIG
DLLSGTYPVVLWQLGNFGQSLLDIDVVVRSTGYWNLEHQPDATVDGLWEKILTGDEATRKTAQQDINRYVIEQAWFAPMV
NPDGFYAHSPDVKIDHVSDHEALTPKLRDFK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=384, Percent_Identity=27.6041666666667, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1789966, Length=321, Percent_Identity=26.791277258567, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787762, Length=434, Percent_Identity=25.8064516129032, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1789887, Length=331, Percent_Identity=23.8670694864048, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787495, Length=402, Percent_Identity=24.8756218905473, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55000; Mature: 55000

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSKLALVASAVALAAPLAALLAGCTGEAAPGAESSAGATKNTLTLAMSADITGWDPSN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
QPGYQGWGPEAIWDTLIKCDAFGKPEADIADKWTVSADGKSFTAHIREGQKFSDGTPVDS
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCH
AAIAASFQYLTSHGGSQGDYKGLKTDAPDAQNITLTWPEPQPMIIQRVCNPKITTKALLD
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHC
SGKVNDKPVGSGPYVLDETATTRGSVYTFTKNESHWNTAAYPYKKLVLKVIESETARVSA
CCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHEEE
LKTGQVDGSLITAASYNEVEASGLNVVTMQGQTTRLLLTDHLGKEVPALGSLKVRQAINM
EECCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEEEEHHCCCCCCCCCCHHHHHHHHH
VFDRDAMVKNLYQGHGKPAYQIFRPGSDAYIDGMADPYPFDVTKAKALMAEAGYASGFDL
HHCHHHHHHHHHHCCCCCEEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCC
SLPTMAGQNHETLMPYVTQQLGLLGIKVKQVPLSGANAIGDLLSGTYPVVLWQLGNFGQS
CCCCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCHH
LLDIDVVVRSTGYWNLEHQPDATVDGLWEKILTGDEATRKTAQQDINRYVIEQAWFAPMV
HHEEEEEEECCCEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NPDGFYAHSPDVKIDHVSDHEALTPKLRDFK
CCCCCEECCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRSKLALVASAVALAAPLAALLAGCTGEAAPGAESSAGATKNTLTLAMSADITGWDPSN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCC
QPGYQGWGPEAIWDTLIKCDAFGKPEADIADKWTVSADGKSFTAHIREGQKFSDGTPVDS
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCH
AAIAASFQYLTSHGGSQGDYKGLKTDAPDAQNITLTWPEPQPMIIQRVCNPKITTKALLD
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHC
SGKVNDKPVGSGPYVLDETATTRGSVYTFTKNESHWNTAAYPYKKLVLKVIESETARVSA
CCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHEEE
LKTGQVDGSLITAASYNEVEASGLNVVTMQGQTTRLLLTDHLGKEVPALGSLKVRQAINM
EECCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEEEEHHCCCCCCCCCCHHHHHHHHH
VFDRDAMVKNLYQGHGKPAYQIFRPGSDAYIDGMADPYPFDVTKAKALMAEAGYASGFDL
HHCHHHHHHHHHHCCCCCEEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCC
SLPTMAGQNHETLMPYVTQQLGLLGIKVKQVPLSGANAIGDLLSGTYPVVLWQLGNFGQS
CCCCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCHH
LLDIDVVVRSTGYWNLEHQPDATVDGLWEKILTGDEATRKTAQQDINRYVIEQAWFAPMV
HHEEEEEEECCCEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NPDGFYAHSPDVKIDHVSDHEALTPKLRDFK
CCCCCEECCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA