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The map label for this gene is mnmG [H]

Identifier: 157964062

GI number: 157964062

Start: 5163240

End: 5165129

Strand: Reverse

Name: mnmG [H]

Synonym: Spea_4251

Alternate gene names: 157964062

Gene position: 5165129-5163240 (Counterclockwise)

Preceding gene: 157964063

Following gene: 157964061

Centisome position: 99.82

GC content: 44.81

Gene sequence:

>1890_bases
ATGCAATTTCATGAACGGTTTGATGTAATTGTTGTTGGTGGTGGTCATGCTGGTACTGAAGCGGCTTTAGCTTCAGCTAG
AATGGGCTCTAAAACACTTTTACTGACCCATAACATAGATACTTTGGGACAAATGTCCTGTAACCCTGCTATCGGCGGCA
TAGGTAAAGGACACTTAGTCAAAGAAATTGATGCACTAGGTGGAGCAATGGCTGTTGCTACCGATTTTGCAGGTATCCAA
TTTCGAACATTGAATTCAAGCAAAGGGCCTGCCGTTCGCGCTACTCGTGCACAAGCCGATAGAGCGCTTTATCGCCATAA
GATCCAAGAGATCTTACAGCACCAAGCTAACCTACGTATTTTTCAGCAAGCAGTAGACGATCTTGTTGTTGAAAACGGAA
AAGTTGTTGGTGTAGTTACACAGATGGGCCTTGCCTTTGAAGCACCTGCGGTTGTCTTAACTGCCGGTACATTCTTAGGT
GGAAAAATCCATATTGGACTCGAAAACTACAGCGGTGGTCGAGCCGGTGATCCACCTGCAATCGCTTTAGCTCATCGATT
AAGAGAGTTACCAATTCGTGTTGGCCGTTTAAAAACTGGCACACCACCACGAATCGATGCTAATACCATCGACTTTTCAC
AGATGACAGAGCAAAAAGGTGATGATCCATTGCCAGTCATGTCTTTCATCGGTGATGTAAACGATCATCCTGAGCAGGTC
TCTTGTCATATCACGCACACTAACGAGCGTACCCACGACATTATTCGTGGCGGATTAGACCGAAGCCCAATGTATTCAGG
CATTATCGAAGGTATTGGTCCACGTTACTGCCCTTCTATCGAAGACAAAGTTAATCGATTTGCTGATAAAACATCTCATC
AAATCTTTATCGAACCTGAAGGGTTAAATACCACTGAAATTTATCCAAACGGTATATCAACCAGTTTACCGTTTGATGTT
CAGCTCAATTTGGTTCGTTCAATCAAGGGCATGGAAAACGCTGAGATCATGCGTCCAGGCTATGCGATTGAGTATGATTA
TTTCGATCCTCGCGATCTTAAAAACTCACTAGAGACCAAAACCATTGCAGGTTTATTCTTTGCTGGTCAGATCAACGGCA
CAACAGGTTACGAAGAAGCGGGTGCACAAGGCCTATTAGCCGGCATGAACGCCTCACTGCAAGTGCAAGGCAAAGAAGCT
TGGTGTCCGCGTCGTGATCAAGCCTATCTAGGTGTTTTAGTTGATGACTTATCGACTTTAGGCACTAAAGAACCGTACCG
CATGTTCACTAGCCGCGCGGAATACCGTTTGCTGCTTCGCGAAGATAACGCCGATCTGCGTTTAACTGAGAAAGGTCGTG
AACTTGGATTAGTCGATGATAATCGTTGGGCACTGTTTAGCGAGAAGATGGAATCGATCGAAACTGAACTACAGCGCTTA
CGCGGTCAGTGGGTCCACCCAAATTCACCCTTGGTTGAAGCGTTGAACCCGCATCTGAATACACCGATCACTCGTGAAGC
TACGTTTGAAGATCTATTGCGTCGTCCAGAAATGGATTACCCGAAACTGATGTCTATTGACGGTTTTGGTCCTTCGCTTG
AAGATCAACGTGCTGCAGAGCAGGTTCAGATCCAAGTTAAGTACTCTGGTTATATTCAGCGTCAGCAAGGCGAGATTGAT
AAGGCGATTCGTCATGAGACTACTCTGCTACCGTTAGATTTGGATTACCAAGAAGTACCTGGTTTATCTAACGAAGTAAT
TGCCAAGATGAATGAACATAAACCTGAAACCATTGGCCAAGCCTCTCGTATTTCAGGTATGACGCCAGCTGCTATTTCAA
TTTTGCTGGTTCATCTTAAAAAACGTGGTTTATTACGTAAAAGCGCTTAG

Upstream 100 bases:

>100_bases
GATCAAAACAAGCAAAATAGATCGTTCACCTTAAGCGAAGCAAATGCTAAAATGATCCGCTCAGAAACAATTTATCCTTT
TACGAATCGAAGGCTGTCGA

Downstream 100 bases:

>100_bases
TTCGCCACATATTCGGTAGTTTTGCATGATAAAGGGAAGCTTTGAGGCTTCCCTTCATCTTTTCAGCACTTCATAATACC
GATTGATTTTTGATTCGCTT

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAVATDFAGIQ
FRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQANLRIFQQAVDDLVVENGKVVGVVTQMGLAFEAPAVVLTAGTFLG
GKIHIGLENYSGGRAGDPPAIALAHRLRELPIRVGRLKTGTPPRIDANTIDFSQMTEQKGDDPLPVMSFIGDVNDHPEQV
SCHITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKVNRFADKTSHQIFIEPEGLNTTEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKTIAGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WCPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDNRWALFSEKMESIETELQRL
RGQWVHPNSPLVEALNPHLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPSLEDQRAAEQVQIQVKYSGYIQRQQGEID
KAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQASRISGMTPAAISILLVHLKKRGLLRKSA

Sequences:

>Translated_629_residues
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAVATDFAGIQ
FRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQANLRIFQQAVDDLVVENGKVVGVVTQMGLAFEAPAVVLTAGTFLG
GKIHIGLENYSGGRAGDPPAIALAHRLRELPIRVGRLKTGTPPRIDANTIDFSQMTEQKGDDPLPVMSFIGDVNDHPEQV
SCHITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKVNRFADKTSHQIFIEPEGLNTTEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKTIAGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WCPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDNRWALFSEKMESIETELQRL
RGQWVHPNSPLVEALNPHLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPSLEDQRAAEQVQIQVKYSGYIQRQQGEID
KAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQASRISGMTPAAISILLVHLKKRGLLRKSA
>Mature_629_residues
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGAMAVATDFAGIQ
FRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQANLRIFQQAVDDLVVENGKVVGVVTQMGLAFEAPAVVLTAGTFLG
GKIHIGLENYSGGRAGDPPAIALAHRLRELPIRVGRLKTGTPPRIDANTIDFSQMTEQKGDDPLPVMSFIGDVNDHPEQV
SCHITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKVNRFADKTSHQIFIEPEGLNTTEIYPNGISTSLPFDV
QLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETKTIAGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEA
WCPRRDQAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDNRWALFSEKMESIETELQRL
RGQWVHPNSPLVEALNPHLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPSLEDQRAAEQVQIQVKYSGYIQRQQGEID
KAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQASRISGMTPAAISILLVHLKKRGLLRKSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=632, Percent_Identity=45.5696202531646, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI19882217, Length=657, Percent_Identity=43.8356164383562, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI183227703, Length=672, Percent_Identity=42.8571428571429, Blast_Score=474, Evalue=1e-133,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=73.6089030206677, Blast_Score=971, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=636, Percent_Identity=40.7232704402516, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321202, Length=627, Percent_Identity=45.933014354067, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24658174, Length=638, Percent_Identity=45.4545454545455, Blast_Score=508, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69385; Mature: 69385

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGAMAVATDFAGIQFRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQANLRI
HHHHHHCCHHEEHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQQAVDDLVVENGKVVGVVTQMGLAFEAPAVVLTAGTFLGGKIHIGLENYSGGRAGDPPA
HHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECEEECCEEEEEEECCCCCCCCCCHH
IALAHRLRELPIRVGRLKTGTPPRIDANTIDFSQMTEQKGDDPLPVMSFIGDVNDHPEQV
HHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCCCCCEE
SCHITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKVNRFADKTSHQIFIEPE
EEEEEECCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLNTTEIYPNGISTSLPFDVQLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETK
CCCCCEECCCCCCCCCCCCEEHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
TIAGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEAWCPRRDQAYLGVLVDDLSTL
HEEEEEEEEEECCCCCCHHCCCCCEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDNRWALFSEKMESIETELQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEECCEECCCHHHHHHHHHHHHHHHHHH
RGQWVHPNSPLVEALNPHLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPSLEDQRAAE
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCE
QVQIQVKYSGYIQRQQGEIDKAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQ
EEEEEEEECCEEEECCCHHHHHHHCCCEEEEECCCHHHCCCCCHHHHHHHHCCCCHHHCC
ASRISGMTPAAISILLVHLKKRGLLRKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQFHERFDVIVVGGGHAGTEAALASARMGSKTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGAMAVATDFAGIQFRTLNSSKGPAVRATRAQADRALYRHKIQEILQHQANLRI
HHHHHHCCHHEEHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQQAVDDLVVENGKVVGVVTQMGLAFEAPAVVLTAGTFLGGKIHIGLENYSGGRAGDPPA
HHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECEEECCEEEEEEECCCCCCCCCCHH
IALAHRLRELPIRVGRLKTGTPPRIDANTIDFSQMTEQKGDDPLPVMSFIGDVNDHPEQV
HHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCCCCCEE
SCHITHTNERTHDIIRGGLDRSPMYSGIIEGIGPRYCPSIEDKVNRFADKTSHQIFIEPE
EEEEEECCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
GLNTTEIYPNGISTSLPFDVQLNLVRSIKGMENAEIMRPGYAIEYDYFDPRDLKNSLETK
CCCCCEECCCCCCCCCCCCEEHHHHHHHCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
TIAGLFFAGQINGTTGYEEAGAQGLLAGMNASLQVQGKEAWCPRRDQAYLGVLVDDLSTL
HEEEEEEEEEECCCCCCHHCCCCCEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GTKEPYRMFTSRAEYRLLLREDNADLRLTEKGRELGLVDDNRWALFSEKMESIETELQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEECCEECCCHHHHHHHHHHHHHHHHHH
RGQWVHPNSPLVEALNPHLNTPITREATFEDLLRRPEMDYPKLMSIDGFGPSLEDQRAAE
CCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCE
QVQIQVKYSGYIQRQQGEIDKAIRHETTLLPLDLDYQEVPGLSNEVIAKMNEHKPETIGQ
EEEEEEEECCEEEECCCHHHHHHHCCCEEEEECCCHHHCCCCCHHHHHHHHCCCCHHHCC
ASRISGMTPAAISILLVHLKKRGLLRKSA
HHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA