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The map label for this gene is gidB [H]

Identifier: 157964061

GI number: 157964061

Start: 5162498

End: 5163121

Strand: Reverse

Name: gidB [H]

Synonym: Spea_4250

Alternate gene names: 157964061

Gene position: 5163121-5162498 (Counterclockwise)

Preceding gene: 157964062

Following gene: 157964060

Centisome position: 99.78

GC content: 44.71

Gene sequence:

>624_bases
GTGTTATCTGCCCAATTAAATGATTATTTGGCCGAAGTTGGCCTGACTGCTACCGAGCAGCAAAAAAAGCAGCTTGTAGA
CTTTGTTGGCATGCTCAATAAGTGGAACAAGGCATTTAACTTAACCTCAGTGCGAGATCCTGAGCAGATGTTAATTCGCC
ATATTATGGACAGCTTAGTGGTGTCTCCGCACCTAAAAGGATCGCGCTTTATCGACGTAGGCACAGGACCTGGATTGCCA
GGGATCCCGCTAGCGATCTTAAATCCAGATAAAGAATTCGTACTGCTCGATAGCTTAGGTAAGCGGATTCGCTTTCAGAA
GCAGGTGCAGTTTGAGCTCGGTATCAACAATATCTCTTCGATTGAAAGTCGTGTAGAGGCTTATCAACCTAAAGACTTGT
TTGACGGTGTATTAAGCCGCGCATTTGCATCTATTCAAGATATGCTGCATTGGTGCCATCACTTACCAAAAGCAGATGGT
TGCTTCTATGCACTGAAAGGCCAACTAAGTGAAGAAGAGATGTCACAGATGCCTGCTGGCTTTGTGGTGACCGATACCAT
AGAACTAAAAGTACCTAAACTTGATGAGCAGCGCCATTTGCTGCGAGTTATCAAGCAAGACTAA

Upstream 100 bases:

>100_bases
GTTTTGCATGATAAAGGGAAGCTTTGAGGCTTCCCTTCATCTTTTCAGCACTTCATAATACCGATTGATTTTTGATTCGC
TTTATTTGAGGACACAAACT

Downstream 100 bases:

>100_bases
CGATCTTTATGCGAGTGGAGAACTATCGCACTCGCAAGCTTATTTTGTCTAAGTGGCGATAGATATATACCGGGTTGAAG
TGAGCTGCTGCTGATAAAGC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLVVSPHLKGSRFIDVGTGPGLP
GIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISSIESRVEAYQPKDLFDGVLSRAFASIQDMLHWCHHLPKADG
CFYALKGQLSEEEMSQMPAGFVVTDTIELKVPKLDEQRHLLRVIKQD

Sequences:

>Translated_207_residues
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLVVSPHLKGSRFIDVGTGPGLP
GIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISSIESRVEAYQPKDLFDGVLSRAFASIQDMLHWCHHLPKADG
CFYALKGQLSEEEMSQMPAGFVVTDTIELKVPKLDEQRHLLRVIKQD
>Mature_207_residues
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLVVSPHLKGSRFIDVGTGPGLP
GIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISSIESRVEAYQPKDLFDGVLSRAFASIQDMLHWCHHLPKADG
CFYALKGQLSEEEMSQMPAGFVVTDTIELKVPKLDEQRHLLRVIKQD

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=203, Percent_Identity=57.6354679802956, Blast_Score=255, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 23386; Mature: 23386

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLV
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VSPHLKGSRFIDVGTGPGLPGIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISS
CCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHEECCCHHHH
IESRVEAYQPKDLFDGVLSRAFASIQDMLHWCHHLPKADGCFYALKGQLSEEEMSQMPAG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCC
FVVTDTIELKVPKLDEQRHLLRVIKQD
EEEEEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLSAQLNDYLAEVGLTATEQQKKQLVDFVGMLNKWNKAFNLTSVRDPEQMLIRHIMDSLV
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VSPHLKGSRFIDVGTGPGLPGIPLAILNPDKEFVLLDSLGKRIRFQKQVQFELGINNISS
CCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHEECCCHHHH
IESRVEAYQPKDLFDGVLSRAFASIQDMLHWCHHLPKADGCFYALKGQLSEEEMSQMPAG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCC
FVVTDTIELKVPKLDEQRHLLRVIKQD
EEEEEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA