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The map label for this gene is mleN [H]

Identifier: 157963976

GI number: 157963976

Start: 5056193

End: 5057599

Strand: Direct

Name: mleN [H]

Synonym: Spea_4165

Alternate gene names: 157963976

Gene position: 5056193-5057599 (Clockwise)

Preceding gene: 157963975

Following gene: 157963977

Centisome position: 97.71

GC content: 49.54

Gene sequence:

>1407_bases
ATGTCTCAGTCAACTAAGCTTGAGTTTCATATTGGAAAAATTGGTGGAATGGTTCCACTATTGACCATGGTCGGTCTAAT
GTTATTGCTTACCTTTAATGGTGTGGGTGGCCCTAAAGCCGCTTGGGCACCTGGCTTTATCGCCATTATTTTAGGTATGT
TCCTAGTTAAGAGTAAGCGCGATTATTTTCAATCTATTTTAGACGGCATCGCCGATAAAACCGGTGTTGTGGTGATCACA
GCCTGGATCTTCGCAGCCGTACTCGGCTCCTTAATGAAGGCCGGCGGCCTAGTAGACGGTATCTTGTGGTTTGGCATGTC
GACCGGTATTCAAGGCAGCATGTTCCTTATCATCGTCTTTATGTCTACCGCTATCTTCGCCATGGGCACAGGTTCTTCAA
ACGGTGCTAACTTAGCACTCGCGCCAATTTTGTTCCCAGCGGGTGTTTACTTAGGCGCAGATCCTGCCTTCGTTGCCCTA
GCCCTACTATCGGGTGCGGTATTTGGCGATAACGTTGCCCCAATTTCAGATACTACCATCGTAGGCTGTACCACACAGGG
AGCCGATTTCGGCGCCACGGTTCGCCAACGTATGCCGCTTTCACTAACAGCCGGCGCTATCGCACTGGTTGCCTTGGTAG
TCTTTGGTCAAGGTGATGGCTTAGTCGAAGCTGCCACTCAAAATGGTGACAGTGCACGCCCAGAAAGCTTACTTATGCTG
CTCTCAGTTGCTGCCGTTGTGTTCTTTGCACTGAAGGGGCGTCACCTGTTAGAAGCCCTAAGCTACGGTATCCTTGTTGC
GACAGTTGTTGGACTTGGCATTGGTGCTTTCGCGGTATCGGATCTGTTCACCGTTCCATCTGAGCGCGGCGTATCGAATG
GCATCATCGAAGATGCGATTGCTGGCGTGACGGGTGCAGTGGTCTTCGTAATCCTAATCTTAGGTGTTATCAAGATTTTC
ATGAATAGCGGCATTATGGATGACATTCTTGCCTTCATCACCGAGCGCGTTTCAAGCAGCACTCGCAGCACCGAGCTATT
AATGTTCTTCTCGACTTCAGTGTCATCACTATTAGTTAGTTCGAACGCACCATCTCAACTGCTAGTTGGCCCAACTCTGG
TTAAGCCATTAGGAGAGAAGAACAACATTACTCCTGAGCGCCGCGCTAACCTAATGAGCGCCTCAGTATGTTCTATCTTC
TACATGATGCCTTGGTGTCTTGCGGTGATGGTTTGGTACTCAGCGGTAGAAACATCAGCCATCTCTTCTGGCTTAGCGGT
TCCGAGTGCCTCGATCAGCTTTATGGCACCTTACCCTTGGGCGCTACTGGGTGTGATGATGTTCTCTATCATCACAGGTT
GGAAACGCTACGGTAATCAGGCTCAAGCTCAAGTACAGCCAGCCTAA

Upstream 100 bases:

>100_bases
AAAAAACATGCCTGAGGGTCTTCTCAGGCATTTTGGCCAAATTTTCATTCCTCGTCGCCACTGTCCCTACAGCCATGACG
AAGGAATAGGTAGAATCATT

Downstream 100 bases:

>100_bases
TTAATTGCATTAAACCAATAACAATAACTGTGGCGGCGCAGTCCGCCACAAGTAAGAAAATAGGAAATAGAAATGACTAA
AGCACTTAACACTGAATCAG

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKRDYFQSILDGIADKTGVVVIT
AWIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVFMSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVAL
ALLSGAVFGDNVAPISDTTIVGCTTQGADFGATVRQRMPLSLTAGAIALVALVVFGQGDGLVEAATQNGDSARPESLLML
LSVAAVVFFALKGRHLLEALSYGILVATVVGLGIGAFAVSDLFTVPSERGVSNGIIEDAIAGVTGAVVFVILILGVIKIF
MNSGIMDDILAFITERVSSSTRSTELLMFFSTSVSSLLVSSNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIF
YMMPWCLAVMVWYSAVETSAISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGNQAQAQVQPA

Sequences:

>Translated_468_residues
MSQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKRDYFQSILDGIADKTGVVVIT
AWIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVFMSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVAL
ALLSGAVFGDNVAPISDTTIVGCTTQGADFGATVRQRMPLSLTAGAIALVALVVFGQGDGLVEAATQNGDSARPESLLML
LSVAAVVFFALKGRHLLEALSYGILVATVVGLGIGAFAVSDLFTVPSERGVSNGIIEDAIAGVTGAVVFVILILGVIKIF
MNSGIMDDILAFITERVSSSTRSTELLMFFSTSVSSLLVSSNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIF
YMMPWCLAVMVWYSAVETSAISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGNQAQAQVQPA
>Mature_467_residues
SQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKRDYFQSILDGIADKTGVVVITA
WIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVFMSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVALA
LLSGAVFGDNVAPISDTTIVGCTTQGADFGATVRQRMPLSLTAGAIALVALVVFGQGDGLVEAATQNGDSARPESLLMLL
SVAAVVFFALKGRHLLEALSYGILVATVVGLGIGAFAVSDLFTVPSERGVSNGIIEDAIAGVTGAVVFVILILGVIKIFM
NSGIMDDILAFITERVSSSTRSTELLMFFSTSVSSLLVSSNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIFY
MMPWCLAVMVWYSAVETSAISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGNQAQAQVQPA

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 48756; Mature: 48625

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKR
CCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DYFQSILDGIADKTGVVVITAWIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
MSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVALALLSGAVFGDNVAPISDTTI
HHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCCCHHHHHHHHHHCHHCCCCCCCCCCCEE
VGCTTQGADFGATVRQRMPLSLTAGAIALVALVVFGQGDGLVEAATQNGDSARPESLLML
EEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCCCHHHHHHH
LSVAAVVFFALKGRHLLEALSYGILVATVVGLGIGAFAVSDLFTVPSERGVSNGIIEDAI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AGVTGAVVFVILILGVIKIFMNSGIMDDILAFITERVSSSTRSTELLMFFSTSVSSLLVS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHC
SNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIFYMMPWCLAVMVWYSAVETSA
CCCCCCEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGNQAQAQVQPA
HHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SQSTKLEFHIGKIGGMVPLLTMVGLMLLLTFNGVGGPKAAWAPGFIAIILGMFLVKSKR
CCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DYFQSILDGIADKTGVVVITAWIFAAVLGSLMKAGGLVDGILWFGMSTGIQGSMFLIIVF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
MSTAIFAMGTGSSNGANLALAPILFPAGVYLGADPAFVALALLSGAVFGDNVAPISDTTI
HHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCCCHHHHHHHHHHCHHCCCCCCCCCCCEE
VGCTTQGADFGATVRQRMPLSLTAGAIALVALVVFGQGDGLVEAATQNGDSARPESLLML
EEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCCCHHHHHHH
LSVAAVVFFALKGRHLLEALSYGILVATVVGLGIGAFAVSDLFTVPSERGVSNGIIEDAI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AGVTGAVVFVILILGVIKIFMNSGIMDDILAFITERVSSSTRSTELLMFFSTSVSSLLVS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHC
SNAPSQLLVGPTLVKPLGEKNNITPERRANLMSASVCSIFYMMPWCLAVMVWYSAVETSA
CCCCCCEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSGLAVPSASISFMAPYPWALLGVMMFSIITGWKRYGNQAQAQVQPA
HHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]