Click here to switch to the map view.

The map label for this gene is mscM [H]

Identifier: 157963969

GI number: 157963969

Start: 5049203

End: 5050462

Strand: Reverse

Name: mscM [H]

Synonym: Spea_4158

Alternate gene names: 157963969

Gene position: 5050462-5049203 (Counterclockwise)

Preceding gene: 157963978

Following gene: 157963966

Centisome position: 97.6

GC content: 45.56

Gene sequence:

>1260_bases
ATGACTTTGGATCAGGAATTACGCGCTGCGATTGCTCAGTGGTTAACTAATATTGGCATCAACACTCAACCGTCTGATGG
CGTGTCGAGCGCGATTATGGTGCTGGCATGTTTTGTGGTGGCTTTTATCGGTTATTTCTTCGTAAAACGCGGAGTGGTGA
CCACCATGAATATGGTTATTCTGCGTTCTAAGGCGCATTGGGATGACATCTTTATGCGTCATCAAGTATTGGAGAAGATA
GCCATACTTGTACCCATTATTATCTTGAATATTTTGTTGCCGCTGGCCTTGAATGACCACAAGGTTCTTAGCAGTCTAAG
CGATCGCCTGCTCGATGTGACTATCGTTATCTTGTTTGTTCGCGCGGTTTATAGCGCCTTGGACGCAGCTAATGAGATAG
CCGATTATAATTTAGTCAGCCGTAGGTTGCCGATTAAGAGCTTTGTACAGCTGGCTAAACTCTTCATGTTTTTCGTCGCT
ATCATCATCTCAATCTCGGTTTTAGCAGACCAATCGCCAGTCTATTTCTTAAGTGGTTTAGGTGTTGCAACCGGTTTAGT
CATGTTGGTATTTAGGGATACTATCTTGGGCTTTGTGGCGGGGATTCAGCTGGCTGCTAACCGTATGGTGAGCCCCGGAG
ACTGGATCGAGATGGATAACTTTGGCGCTAACGGTACGGTTGAAGAGGTGTCGTTGACCACGGTTAAGGTACGTAACTTT
GACCAAACACTGACCATGCTACCAGCTTACGCTTTAACTTCTGGCTCCTTTAGAAACTGGCGTGGCATGTCTGAATCCGG
TGGCCGTAGAATTAAGCGCGCGCTACAGATAGATATTCAATCGGTGCGTTTTTTAACTGAAGAGGATATGCGCAGTTTAA
GCAAGATCAATCATCTTAAGGACTATTTCTCTGCTAAATCGGCGGAGATCATTACATATAACGCTCAAGTTGAAGATGCG
GATATGTACGTGAATAGTCGTCATCTAACCAATCTAGGTACCTTTAGAGCTTATGTTCAGGAGTACCTGCAACAGCACGA
TAAGATCCGTAAAGATATGACACTGATGGTACGCCAGCTCGCGCCGACCGAACTCGGTGTACCAATCGAGCTTTATATCT
TTACTAACGATACCCGCTGGGAATTTTACGAGGGGATCCAAGCCGATATTTTCGATCATATCTTTTCGATTCTGCCAGAG
TTTGGCTTGCGAGTATTCCAAGGGCCTACAGGAGCGGATGTGCGCAGCTTAAAGCATTAG

Upstream 100 bases:

>100_bases
TATAATAGATGTTTGTAAGGGTTATGTTTCAACCTATCGAGGTTAATGTTGATAGGTTTTGACTCGTTGAATGCTCATTT
GAGCGCGCAAGAATTGGAGA

Downstream 100 bases:

>100_bases
TGCGTTAAGCTCGCACTAAAAAGCCAAGTTAAAACTAATGAGTATTTTAGTATTAAACTTGGCTTTTTGTTTTGTGGCTT
ACAGATAAACTAGTTTTCGC

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MTLDQELRAAIAQWLTNIGINTQPSDGVSSAIMVLACFVVAFIGYFFVKRGVVTTMNMVILRSKAHWDDIFMRHQVLEKI
AILVPIIILNILLPLALNDHKVLSSLSDRLLDVTIVILFVRAVYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVA
IIISISVLADQSPVYFLSGLGVATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNF
DQTLTMLPAYALTSGSFRNWRGMSESGGRRIKRALQIDIQSVRFLTEEDMRSLSKINHLKDYFSAKSAEIITYNAQVEDA
DMYVNSRHLTNLGTFRAYVQEYLQQHDKIRKDMTLMVRQLAPTELGVPIELYIFTNDTRWEFYEGIQADIFDHIFSILPE
FGLRVFQGPTGADVRSLKH

Sequences:

>Translated_419_residues
MTLDQELRAAIAQWLTNIGINTQPSDGVSSAIMVLACFVVAFIGYFFVKRGVVTTMNMVILRSKAHWDDIFMRHQVLEKI
AILVPIIILNILLPLALNDHKVLSSLSDRLLDVTIVILFVRAVYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVA
IIISISVLADQSPVYFLSGLGVATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNF
DQTLTMLPAYALTSGSFRNWRGMSESGGRRIKRALQIDIQSVRFLTEEDMRSLSKINHLKDYFSAKSAEIITYNAQVEDA
DMYVNSRHLTNLGTFRAYVQEYLQQHDKIRKDMTLMVRQLAPTELGVPIELYIFTNDTRWEFYEGIQADIFDHIFSILPE
FGLRVFQGPTGADVRSLKH
>Mature_418_residues
TLDQELRAAIAQWLTNIGINTQPSDGVSSAIMVLACFVVAFIGYFFVKRGVVTTMNMVILRSKAHWDDIFMRHQVLEKIA
ILVPIIILNILLPLALNDHKVLSSLSDRLLDVTIVILFVRAVYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVAI
IISISVLADQSPVYFLSGLGVATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNFD
QTLTMLPAYALTSGSFRNWRGMSESGGRRIKRALQIDIQSVRFLTEEDMRSLSKINHLKDYFSAKSAEIITYNAQVEDAD
MYVNSRHLTNLGTFRAYVQEYLQQHDKIRKDMTLMVRQLAPTELGVPIELYIFTNDTRWEFYEGIQADIFDHIFSILPEF
GLRVFQGPTGADVRSLKH

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=393, Percent_Identity=44.0203562340967, Blast_Score=335, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 47251; Mature: 47119

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDQELRAAIAQWLTNIGINTQPSDGVSSAIMVLACFVVAFIGYFFVKRGVVTTMNMVI
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRSKAHWDDIFMRHQVLEKIAILVPIIILNILLPLALNDHKVLSSLSDRLLDVTIVILFV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RAVYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVAIIISISVLADQSPVYFLSGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GVATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHEEEEEEEECCH
DQTLTMLPAYALTSGSFRNWRGMSESGGRRIKRALQIDIQSVRFLTEEDMRSLSKINHLK
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYFSAKSAEIITYNAQVEDADMYVNSRHLTNLGTFRAYVQEYLQQHDKIRKDMTLMVRQL
HHHCCCCCEEEEEECEECHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APTELGVPIELYIFTNDTRWEFYEGIQADIFDHIFSILPEFGLRVFQGPTGADVRSLKH
CCCCCCCCEEEEEEECCCCHHHHHCHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHCCC
>Mature Secondary Structure 
TLDQELRAAIAQWLTNIGINTQPSDGVSSAIMVLACFVVAFIGYFFVKRGVVTTMNMVI
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRSKAHWDDIFMRHQVLEKIAILVPIIILNILLPLALNDHKVLSSLSDRLLDVTIVILFV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RAVYSALDAANEIADYNLVSRRLPIKSFVQLAKLFMFFVAIIISISVLADQSPVYFLSGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GVATGLVMLVFRDTILGFVAGIQLAANRMVSPGDWIEMDNFGANGTVEEVSLTTVKVRNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHEEEEEEEECCH
DQTLTMLPAYALTSGSFRNWRGMSESGGRRIKRALQIDIQSVRFLTEEDMRSLSKINHLK
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYFSAKSAEIITYNAQVEDADMYVNSRHLTNLGTFRAYVQEYLQQHDKIRKDMTLMVRQL
HHHCCCCCEEEEEECEECHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APTELGVPIELYIFTNDTRWEFYEGIQADIFDHIFSILPEFGLRVFQGPTGADVRSLKH
CCCCCCCCEEEEEEECCCCHHHHHCHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]