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The map label for this gene is hycF [C]

Identifier: 157963951

GI number: 157963951

Start: 5030254

End: 5031933

Strand: Reverse

Name: hycF [C]

Synonym: Spea_4140

Alternate gene names: 157963951

Gene position: 5031933-5030254 (Counterclockwise)

Preceding gene: 157963952

Following gene: 157963950

Centisome position: 97.24

GC content: 46.9

Gene sequence:

>1680_bases
GTGAGCAATAGTGAAGCACAAGTTGGACTCAAAGAGATTCGACAGAAGGTCCTTGCTGGGACTCAGGTCATTCAAAACCT
GATCCCACCGACCGTAAGTTACACCACCGAGGGTAACGTCCTCGTTATCGGGCCAGAAGATTTGGCTCGCTTAGCGGCTG
ATAAGTTATCGACTATGGGTAAGTGTGCCATTCTGGCAAATGAGCCTATTACCAGCCAAGATGAAGCCCACCTTGAAAAA
GTAATGAACGCTGCTGAAAACGTAGAAAGTTATTACAACAAGCTAGTCGAAATCAAAGGTTTCTTAGGCCAATTCCAAGT
CAAGGTTGAGCACGACAACGGTAACGCTGATTTAAGTACGGTGGCAATCCGTAAAGCACATTTCGACTTGATCCTTGATT
TGAGTATGAGCCCTTGTATCAACCTAGAAATGTTGCCTCCAGGCTATTTCTACGTGGGTCAAGACCAAGCTAAGCTTGAA
GATGCGCTTGAACAGTTACCAGATCTTATAGGCTCATTTGATAAGCCTCGTTACGTTAAAATTAACAGCGATATCTGTGC
CCATAACCGTAATGGTAATGAAGGCTGTACGCGTTGCTTAAACTTCTGCCCAGCTGATGCGATTTCTAGCGTCGCGAAGA
AGATCGAAATTGACCCATACCTTTGTCATGGCGCAGGTAGCTGTACTAATGCGTGTCCAACTGGCGCGATTAGCTATGAC
TTGCCAACGCCGCAAGCTATGCATACTTATCTGCAAAAGCTAGTGACTAAGTTCCGTGCTCAAGCGCAGGTCGCACCGGT
AATCCTTTTCCATGATATGGGCAAAGGCGCACAGTTGGTGACCGAAACGTTAGCGGGTGACATCATTCCAATCGAAATGG
AAGAGATCACCGTTGCTAGTATGGATCATTGGTTCTCGGCTCTAGCATGGGGCGCGCGCCAAATTTTGGTTTTGCAAACT
GATGTGACTGCACCAACATTAACCCAGATGCTTAACGGTGAGCTTTCATTAGCCAACGATATTCTTGATGAAATGGGCCA
GCCACAACGCATTAGCCTCATCACCGAAGCGCATTTGAATGAAGCGGGTGCTAGCGAGCTATCTGAGCTACTTAATACAA
GCGCTAGCTGGCCTGTGATTGTGCCTGCTGAATTTGCAGCCACAACCAAGCGTGAAACGATTTACAACGCAATCGATCAT
CTTAATGAGCAAGCTGCATCGGTTGATACCTGTTTATCTCAAAGCAATATCCCATACGGTCAAGTCAGCGTTAACACAGA
GAACTGTACCTTGTGTATGTCTTGTGTGGCGACCTGTCCAACAATGGCATTAACAGACGGTGGTGACCGTCCTGCATTGC
ACTTTGTTGAGCAAGATTGTGTGCAGTGTGGATTATGTGAAACAGCTTGTCCTGAGAAAGTCATCAGCTTAACGCCTCAG
GTTAATTTCGACAAAGCTGCGCGTCAGGAGCGTCAAACCCTGCATGAGGAAGCCCCGTTCGAATGCATTACTTGCGGCAC
TCCGTTCGCAACGCAATCCATGGTGAAACGGATGTTAGATGTGGTTGGAACCCACAGTGCCTTCAGTGCCAACACAGAGC
GTCTAAAGATGTGTGGTGACTGTCGAGTTAAAGACATGTTTGAAGACATTCTTCAAGATCCTGAAAAGCAACTGCGATAA

Upstream 100 bases:

>100_bases
CTGTAATAGTGAGTAATAAAAGCTGGTGCCAATTATAGGCATATAAAAAACGGCAACACTCATATACAGAAACAGCCAAA
GAACTGTTCAGGAACGCAAT

Downstream 100 bases:

>100_bases
GAGTTTAACCATGACCGAATTAGTAAGAGAAGTTTCAGAAAACGATCAGTTAAGAGCCGATATTTATCAGTTGTTAGCTG
CGTTATTACGTCGCCAACCA

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: 4Fe-4S Ferredoxin; 4fe-4S Ferredoxin; Methyl-Viologen-Reducing Hydrogenase Delta Subunit; NAD(P)H-Quinone Oxidoreductase Chain I; Polyferredoxins; Ferridoxin Protein

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSNSEAQVGLKEIRQKVLAGTQVIQNLIPPTVSYTTEGNVLVIGPEDLARLAADKLSTMGKCAILANEPITSQDEAHLEK
VMNAAENVESYYNKLVEIKGFLGQFQVKVEHDNGNADLSTVAIRKAHFDLILDLSMSPCINLEMLPPGYFYVGQDQAKLE
DALEQLPDLIGSFDKPRYVKINSDICAHNRNGNEGCTRCLNFCPADAISSVAKKIEIDPYLCHGAGSCTNACPTGAISYD
LPTPQAMHTYLQKLVTKFRAQAQVAPVILFHDMGKGAQLVTETLAGDIIPIEMEEITVASMDHWFSALAWGARQILVLQT
DVTAPTLTQMLNGELSLANDILDEMGQPQRISLITEAHLNEAGASELSELLNTSASWPVIVPAEFAATTKRETIYNAIDH
LNEQAASVDTCLSQSNIPYGQVSVNTENCTLCMSCVATCPTMALTDGGDRPALHFVEQDCVQCGLCETACPEKVISLTPQ
VNFDKAARQERQTLHEEAPFECITCGTPFATQSMVKRMLDVVGTHSAFSANTERLKMCGDCRVKDMFEDILQDPEKQLR

Sequences:

>Translated_559_residues
MSNSEAQVGLKEIRQKVLAGTQVIQNLIPPTVSYTTEGNVLVIGPEDLARLAADKLSTMGKCAILANEPITSQDEAHLEK
VMNAAENVESYYNKLVEIKGFLGQFQVKVEHDNGNADLSTVAIRKAHFDLILDLSMSPCINLEMLPPGYFYVGQDQAKLE
DALEQLPDLIGSFDKPRYVKINSDICAHNRNGNEGCTRCLNFCPADAISSVAKKIEIDPYLCHGAGSCTNACPTGAISYD
LPTPQAMHTYLQKLVTKFRAQAQVAPVILFHDMGKGAQLVTETLAGDIIPIEMEEITVASMDHWFSALAWGARQILVLQT
DVTAPTLTQMLNGELSLANDILDEMGQPQRISLITEAHLNEAGASELSELLNTSASWPVIVPAEFAATTKRETIYNAIDH
LNEQAASVDTCLSQSNIPYGQVSVNTENCTLCMSCVATCPTMALTDGGDRPALHFVEQDCVQCGLCETACPEKVISLTPQ
VNFDKAARQERQTLHEEAPFECITCGTPFATQSMVKRMLDVVGTHSAFSANTERLKMCGDCRVKDMFEDILQDPEKQLR
>Mature_558_residues
SNSEAQVGLKEIRQKVLAGTQVIQNLIPPTVSYTTEGNVLVIGPEDLARLAADKLSTMGKCAILANEPITSQDEAHLEKV
MNAAENVESYYNKLVEIKGFLGQFQVKVEHDNGNADLSTVAIRKAHFDLILDLSMSPCINLEMLPPGYFYVGQDQAKLED
ALEQLPDLIGSFDKPRYVKINSDICAHNRNGNEGCTRCLNFCPADAISSVAKKIEIDPYLCHGAGSCTNACPTGAISYDL
PTPQAMHTYLQKLVTKFRAQAQVAPVILFHDMGKGAQLVTETLAGDIIPIEMEEITVASMDHWFSALAWGARQILVLQTD
VTAPTLTQMLNGELSLANDILDEMGQPQRISLITEAHLNEAGASELSELLNTSASWPVIVPAEFAATTKRETIYNAIDHL
NEQAASVDTCLSQSNIPYGQVSVNTENCTLCMSCVATCPTMALTDGGDRPALHFVEQDCVQCGLCETACPEKVISLTPQV
NFDKAARQERQTLHEEAPFECITCGTPFATQSMVKRMLDVVGTHSAFSANTERLKMCGDCRVKDMFEDILQDPEKQLR

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 61053; Mature: 60922

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSEAQVGLKEIRQKVLAGTQVIQNLIPPTVSYTTEGNVLVIGPEDLARLAADKLSTMG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECHHHHHHHHHHHHHHCC
KCAILANEPITSQDEAHLEKVMNAAENVESYYNKLVEIKGFLGQFQVKVEHDNGNADLST
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHH
VAIRKAHFDLILDLSMSPCINLEMLPPGYFYVGQDQAKLEDALEQLPDLIGSFDKPRYVK
HHHHHHHEEEEEECCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEE
INSDICAHNRNGNEGCTRCLNFCPADAISSVAKKIEIDPYLCHGAGSCTNACPTGAISYD
ECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEECC
LPTPQAMHTYLQKLVTKFRAQAQVAPVILFHDMGKGAQLVTETLAGDIIPIEMEEITVAS
CCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHCCCEEEEEHHHHHHHH
MDHWFSALAWGARQILVLQTDVTAPTLTQMLNGELSLANDILDEMGQPQRISLITEAHLN
HHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHCC
EAGASELSELLNTSASWPVIVPAEFAATTKRETIYNAIDHLNEQAASVDTCLSQSNIPYG
CCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QVSVNTENCTLCMSCVATCPTMALTDGGDRPALHFVEQDCVQCGLCETACPEKVISLTPQ
EEEECCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
VNFDKAARQERQTLHEEAPFECITCGTPFATQSMVKRMLDVVGTHSAFSANTERLKMCGD
CCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCHHHHHHCCC
CRVKDMFEDILQDPEKQLR
CCHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
SNSEAQVGLKEIRQKVLAGTQVIQNLIPPTVSYTTEGNVLVIGPEDLARLAADKLSTMG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECHHHHHHHHHHHHHHCC
KCAILANEPITSQDEAHLEKVMNAAENVESYYNKLVEIKGFLGQFQVKVEHDNGNADLST
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHH
VAIRKAHFDLILDLSMSPCINLEMLPPGYFYVGQDQAKLEDALEQLPDLIGSFDKPRYVK
HHHHHHHEEEEEECCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEE
INSDICAHNRNGNEGCTRCLNFCPADAISSVAKKIEIDPYLCHGAGSCTNACPTGAISYD
ECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEECC
LPTPQAMHTYLQKLVTKFRAQAQVAPVILFHDMGKGAQLVTETLAGDIIPIEMEEITVAS
CCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHCCCEEEEEHHHHHHHH
MDHWFSALAWGARQILVLQTDVTAPTLTQMLNGELSLANDILDEMGQPQRISLITEAHLN
HHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHCC
EAGASELSELLNTSASWPVIVPAEFAATTKRETIYNAIDHLNEQAASVDTCLSQSNIPYG
CCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QVSVNTENCTLCMSCVATCPTMALTDGGDRPALHFVEQDCVQCGLCETACPEKVISLTPQ
EEEECCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
VNFDKAARQERQTLHEEAPFECITCGTPFATQSMVKRMLDVVGTHSAFSANTERLKMCGD
CCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCHHHHHHCCC
CRVKDMFEDILQDPEKQLR
CCHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA