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The map label for this gene is fdhB1 [H]

Identifier: 157963947

GI number: 157963947

Start: 5025715

End: 5026302

Strand: Reverse

Name: fdhB1 [H]

Synonym: Spea_4136

Alternate gene names: 157963947

Gene position: 5026302-5025715 (Counterclockwise)

Preceding gene: 157963948

Following gene: 157963946

Centisome position: 97.13

GC content: 50.51

Gene sequence:

>588_bases
ATGGCAGTCATGAAATTCTTATGTGACACCAAGCGCTGCATCGAGTGTAACGGTTGCGTCACAGCATGTAAAAATGAAAA
CGCCTCAGCCATTGGCTGGGGGATCCAGCGTCGTCGCGTGGTTACACTTAATGATGGTGAGCCAGGTGAAGCGTCAATCT
CGGTAGCGTGTATGCACTGTACCGATGCACCTTGTATGGCCGTTTGTCCGGCAGATTGTTTCTACCGTACCGAAGACGGC
ATCGTCTTACACAACAAAGAAACTTGTATCGGTTGTGGTTACTGCTTCTATGCTTGTCCTTTCGGAGCGCCTCAGTTCCC
TCAGAAGAATGCTTTTGGTAGTCGCGGTAAGATGGATAAGTGTACTTTCTGTGCCGGTGGCCCAGAGCCTGATTTCTCAG
AAGCTGAGCGTAAGCTTTACGGTGCAAACCGTATTGCCGAAGGTAAGCTACCTATGTGTGCAGAGCTTTGTGCGACTAAA
GCACTGTTAGCAGGCGATGCGGAGGTTGTTTCTAATATCTTCCGTGAACGCTCTGCTGCACGTGGTGCACCTGGTGCTAT
CTGGGGTTACACCCCTACAACTGGCTAG

Upstream 100 bases:

>100_bases
TGACCTATGGTTATGATGTTGTGACTCAGATGCAAGAGACGAAAGTGAGCTTGTGTCAGGTCAGCAAAGCCTAATTAACT
TGATGAGGAGATAAGCCATT

Downstream 100 bases:

>100_bases
TCTGGCTGAATAGGGCTACGGACTTTGGTCTGTAGCCCGATAAAGGAGTGAGTATGTTTATGAAATCACTGCGCAGCCTG
TTTGCATTGCTAGTTATTCC

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MAVMKFLCDTKRCIECNGCVTACKNENASAIGWGIQRRRVVTLNDGEPGEASISVACMHCTDAPCMAVCPADCFYRTEDG
IVLHNKETCIGCGYCFYACPFGAPQFPQKNAFGSRGKMDKCTFCAGGPEPDFSEAERKLYGANRIAEGKLPMCAELCATK
ALLAGDAEVVSNIFRERSAARGAPGAIWGYTPTTG

Sequences:

>Translated_195_residues
MAVMKFLCDTKRCIECNGCVTACKNENASAIGWGIQRRRVVTLNDGEPGEASISVACMHCTDAPCMAVCPADCFYRTEDG
IVLHNKETCIGCGYCFYACPFGAPQFPQKNAFGSRGKMDKCTFCAGGPEPDFSEAERKLYGANRIAEGKLPMCAELCATK
ALLAGDAEVVSNIFRERSAARGAPGAIWGYTPTTG
>Mature_194_residues
AVMKFLCDTKRCIECNGCVTACKNENASAIGWGIQRRRVVTLNDGEPGEASISVACMHCTDAPCMAVCPADCFYRTEDGI
VLHNKETCIGCGYCFYACPFGAPQFPQKNAFGSRGKMDKCTFCAGGPEPDFSEAERKLYGANRIAEGKLPMCAELCATKA
LLAGDAEVVSNIFRERSAARGAPGAIWGYTPTTG

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=192, Percent_Identity=32.8125, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787122, Length=192, Percent_Identity=30.7291666666667, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1789370, Length=213, Percent_Identity=28.169014084507, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI226510944, Length=187, Percent_Identity=33.1550802139037, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790326, Length=193, Percent_Identity=30.0518134715026, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=185, Percent_Identity=31.3513513513514, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787749, Length=198, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI1789079, Length=101, Percent_Identity=39.6039603960396, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI2367245, Length=186, Percent_Identity=30.6451612903226, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788811, Length=126, Percent_Identity=30.1587301587302, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI87082114, Length=91, Percent_Identity=36.2637362637363, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI87082180, Length=184, Percent_Identity=28.804347826087, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 20869; Mature: 20738

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
12.3 %Cys+Met (Translated Protein)
9.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
11.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVMKFLCDTKRCIECNGCVTACKNENASAIGWGIQRRRVVTLNDGEPGEASISVACMHC
CHHHHHHHCCCCEEECCCCEEECCCCCCCEEECCCCCCEEEEECCCCCCCCEEEEEEEEC
TDAPCMAVCPADCFYRTEDGIVLHNKETCIGCGYCFYACPFGAPQFPQKNAFGSRGKMDK
CCCCEEEECCCHHEEECCCCEEEECCCCEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEPDFSEAERKLYGANRIAEGKLPMCAELCATKALLAGDAEVVSNIFRERSAA
EEEECCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
RGAPGAIWGYTPTTG
CCCCCCEECCCCCCC
>Mature Secondary Structure 
AVMKFLCDTKRCIECNGCVTACKNENASAIGWGIQRRRVVTLNDGEPGEASISVACMHC
HHHHHHHCCCCEEECCCCEEECCCCCCCEEECCCCCCEEEEECCCCCCCCEEEEEEEEC
TDAPCMAVCPADCFYRTEDGIVLHNKETCIGCGYCFYACPFGAPQFPQKNAFGSRGKMDK
CCCCEEEECCCHHEEECCCCEEEECCCCEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEPDFSEAERKLYGANRIAEGKLPMCAELCATKALLAGDAEVVSNIFRERSAA
EEEECCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
RGAPGAIWGYTPTTG
CCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]