Click here to switch to the map view.

The map label for this gene is fdhB1 [H]

Identifier: 157963943

GI number: 157963943

Start: 5020109

End: 5020678

Strand: Reverse

Name: fdhB1 [H]

Synonym: Spea_4132

Alternate gene names: 157963943

Gene position: 5020678-5020109 (Counterclockwise)

Preceding gene: 157963944

Following gene: 157963942

Centisome position: 97.03

GC content: 47.02

Gene sequence:

>570_bases
ATGGCTACAATGAAATTTTTATGTGATACCAAGCGCTGCATCGAGTGTAACGGTTGTGTCACAGCATGTAAGAACGAAAA
CGACTCTGCTCTAGAGTGGGGAATTCAACGTCGTCGCGTAGTGACTATCAATGACGGTGTTAAAGGCGAAGCATCAATCT
CAGTAGCATGTATGCACTGTAGTGATGCACCTTGTATGGCAGTTTGCCCAGCAAACTGTTTCTACAAGACAGAAGATGGC
ATCGTATTGCACGACAAAGATACCTGTATCGGTTGTGGTTACTGCCTATACGCTTGCCCATTTGGCGCGCCACAGTTCCC
TAAATCAGGTGCTTTTGGTGCTCGCGGTAAGATGGATAAGTGTACTTTCTGTGCCGGCGGCCCAGAAGAGAACTTCTCTG
AAGCAGAGCGTCAGAAGTACGGTGCAAACCGTATCGCTGAAGGTAAGCTACCTATGTGTGCTGAGTTGTGTGCAACTAAG
TCATTACTTGCGGGTGACGCAGAAGTTGTTTCTGCTATCTTCCGTGAGCGTGTTGCATACCGTGGTTCTAACAAAAATGT
TTGGGGTTAA

Upstream 100 bases:

>100_bases
CTTATGGTTATGATCCTGTGACTCAGATGCAGGAAACCAAGGCATCGCTTTGTCAGATCACTAAAGCCTAAGTGCAGAAT
TAGCTAGGAGAATTGCCAGA

Downstream 100 bases:

>100_bases
GCCTCAAATATTGCGTTAGTCCATAATCGCTGCAAGCTGCCTCATTGAGGTTGCTTGCAGCAATCAGGAGGTGATATGAA
CAAATGGTTCAAACACATAG

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGVKGEASISVACMHCSDAPCMAVCPANCFYKTEDG
IVLHDKDTCIGCGYCLYACPFGAPQFPKSGAFGARGKMDKCTFCAGGPEENFSEAERQKYGANRIAEGKLPMCAELCATK
SLLAGDAEVVSAIFRERVAYRGSNKNVWG

Sequences:

>Translated_189_residues
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGVKGEASISVACMHCSDAPCMAVCPANCFYKTEDG
IVLHDKDTCIGCGYCLYACPFGAPQFPKSGAFGARGKMDKCTFCAGGPEENFSEAERQKYGANRIAEGKLPMCAELCATK
SLLAGDAEVVSAIFRERVAYRGSNKNVWG
>Mature_188_residues
ATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGVKGEASISVACMHCSDAPCMAVCPANCFYKTEDGI
VLHDKDTCIGCGYCLYACPFGAPQFPKSGAFGARGKMDKCTFCAGGPEENFSEAERQKYGANRIAEGKLPMCAELCATKS
LLAGDAEVVSAIFRERVAYRGSNKNVWG

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=171, Percent_Identity=35.0877192982456, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1787872, Length=174, Percent_Identity=35.0574712643678, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1789370, Length=202, Percent_Identity=30.1980198019802, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI226510944, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790326, Length=192, Percent_Identity=29.6875, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI2367345, Length=184, Percent_Identity=30.9782608695652, Blast_Score=73, Evalue=9e-15,
Organism=Escherichia coli, GI1787749, Length=197, Percent_Identity=27.4111675126904, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87082180, Length=102, Percent_Identity=38.2352941176471, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1788811, Length=126, Percent_Identity=30.952380952381, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI2367245, Length=171, Percent_Identity=30.9941520467836, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87082114, Length=91, Percent_Identity=35.1648351648352, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789079, Length=103, Percent_Identity=34.9514563106796, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 20420; Mature: 20288

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
12.7 %Cys+Met (Translated Protein)
9.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGVKGEASISVACMHC
CCCHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEC
SDAPCMAVCPANCFYKTEDGIVLHDKDTCIGCGYCLYACPFGAPQFPKSGAFGARGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEENFSEAERQKYGANRIAEGKLPMCAELCATKSLLAGDAEVVSAIFRERVAY
EEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGSNKNVWG
CCCCCCCCC
>Mature Secondary Structure 
ATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGVKGEASISVACMHC
CCHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEC
SDAPCMAVCPANCFYKTEDGIVLHDKDTCIGCGYCLYACPFGAPQFPKSGAFGARGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEENFSEAERQKYGANRIAEGKLPMCAELCATKSLLAGDAEVVSAIFRERVAY
EEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGSNKNVWG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]