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The map label for this gene is irlS [H]

Identifier: 157963920

GI number: 157963920

Start: 4995928

End: 4997265

Strand: Reverse

Name: irlS [H]

Synonym: Spea_4109

Alternate gene names: 157963920

Gene position: 4997265-4995928 (Counterclockwise)

Preceding gene: 157963921

Following gene: 157963919

Centisome position: 96.57

GC content: 47.98

Gene sequence:

>1338_bases
GTGCTGGGTATCGGTTACGCCAAGGGCATTAAGCCCTATCAGAGTAGTGCCTGGCGCATTACCATCATATTCTCGGCCCT
GGTAACTGTCATCATTGGCACATTGTTACTGGGAATGTATCGCCAGCTGATCAATGAGCAAGAGTATCAGCTGGACTTGC
ATCTAGACGCCGAAAAGACCCGCTATCAACAGATGGCATTGACCCTAGATAAGCGCAGCTTTGCGACTCAAGTGCGCAGT
GCCGACCCCAAAGTCACCTTGATTGTTTGGCGTGACTCCTCAGATTATATCGGCTCTTTGAGCATGATCCCCGACGATAT
GCCTATGCTACCAGAGAAACGCGAGTTTCCGGTGTTTACTGGTGGCCCTGAAAAACTGCATATCTTAACCGGTGGCATGG
TGATGACTCGCTATGGCCCCATGTTGATCGCGACCCGTGTCGACCAGCTCGGCGCGCTTATCGATAAATTTACCAATGCT
GCGATCACCGCCTTGATGCTCACCGTTGTGCTGACTTTAGCGCTTGGCTATCTATTCTCTAAAGCCATTTTACGTCGCTT
AGTACAGTATAACCGTTTAAGTAAGAAGATTGAGCGAGGACAATATTCGACACGTTTACCCGTGAGCTGGCGTCAAGACG
AATTTGATATGTTGGCTAAGCAATTTAATGGTGTACTCGATGCACTAGAGAAGAACTTAGCAGCAGTACGGGGGGTCACT
GACAATATCGCCCATGATCTGCGTACCCCTTTGTCTCATCTTAGAATCGGGCTTGAGCAATTACCGAGCAAGCCTAAAGA
GGAGCTTGAGGAAAGCTGCGCCATTCTTACCGAAGAACTCGATCATTGTTTAGCGACCTTCGATGCCATGTTGTCACTAA
CCCGTATCGAAGAGGGCCAGCAAACCTTAGAACTGCAAGATGTGAGTTTAATTGCGATTAGCCAAGACTTGTTTGAGATG
GCGGAGGCGATGGCAGAGTTTAATGATCAGTCGTTGAAGCTGACGACCGGTGAAGAGTTTCAGGTCAGCGGCGACAAATA
CTTGCTGTTCCAAGCCTTGTTTAACTTAGTTGATAACGCAATCAAATACTCCGGTGAAGGCGCAGAGATAAGTATTTCAC
AAAATCGCAATGTGATTTCGATTCAAGACAATGGTCCTGGGATCCCCGAGGGCTCTCGTGACAAGGTATTTGAGCGTCTA
GTGCGCCTAGACCCTAGCCGTCATCATAAGGGCACAGGGTTAGGTTTATCTATGGTGAAGGCGATTCTATCGCGACATAA
TGCTAAAATAGCGCTAACCGATAATCAGCCAGGCCTAAAGGTGATTATCACTTTTTAG

Upstream 100 bases:

>100_bases
CACTTCGATCCTCGTACCAATGTCATCGATGTGCATATCGCCAAACTGAGACGTAAGTTTGAAGAGCTAGGCCACGGTGA
ATTAATCGAAACTGTTCGAG

Downstream 100 bases:

>100_bases
TCTTGTGATAGCTAAGCTGCCTTAGAGTGGCTTAGTGGAAGCTTTATTTATGTATCGAGTCATTGCCGATGAGCGCGACT
TTATTGTAATTAATAAGTCC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MLGIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEYQLDLHLDAEKTRYQQMALTLDKRSFATQVRS
ADPKVTLIVWRDSSDYIGSLSMIPDDMPMLPEKREFPVFTGGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNA
AITALMLTVVLTLALGYLFSKAILRRLVQYNRLSKKIERGQYSTRLPVSWRQDEFDMLAKQFNGVLDALEKNLAAVRGVT
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEESCAILTEELDHCLATFDAMLSLTRIEEGQQTLELQDVSLIAISQDLFEM
AEAMAEFNDQSLKLTTGEEFQVSGDKYLLFQALFNLVDNAIKYSGEGAEISISQNRNVISIQDNGPGIPEGSRDKVFERL
VRLDPSRHHKGTGLGLSMVKAILSRHNAKIALTDNQPGLKVIITF

Sequences:

>Translated_445_residues
MLGIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEYQLDLHLDAEKTRYQQMALTLDKRSFATQVRS
ADPKVTLIVWRDSSDYIGSLSMIPDDMPMLPEKREFPVFTGGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNA
AITALMLTVVLTLALGYLFSKAILRRLVQYNRLSKKIERGQYSTRLPVSWRQDEFDMLAKQFNGVLDALEKNLAAVRGVT
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEESCAILTEELDHCLATFDAMLSLTRIEEGQQTLELQDVSLIAISQDLFEM
AEAMAEFNDQSLKLTTGEEFQVSGDKYLLFQALFNLVDNAIKYSGEGAEISISQNRNVISIQDNGPGIPEGSRDKVFERL
VRLDPSRHHKGTGLGLSMVKAILSRHNAKIALTDNQPGLKVIITF
>Mature_445_residues
MLGIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEYQLDLHLDAEKTRYQQMALTLDKRSFATQVRS
ADPKVTLIVWRDSSDYIGSLSMIPDDMPMLPEKREFPVFTGGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNA
AITALMLTVVLTLALGYLFSKAILRRLVQYNRLSKKIERGQYSTRLPVSWRQDEFDMLAKQFNGVLDALEKNLAAVRGVT
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEESCAILTEELDHCLATFDAMLSLTRIEEGQQTLELQDVSLIAISQDLFEM
AEAMAEFNDQSLKLTTGEEFQVSGDKYLLFQALFNLVDNAIKYSGEGAEISISQNRNVISIQDNGPGIPEGSRDKVFERL
VRLDPSRHHKGTGLGLSMVKAILSRHNAKIALTDNQPGLKVIITF

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=331, Percent_Identity=26.5861027190332, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788393, Length=260, Percent_Identity=31.5384615384615, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1788279, Length=280, Percent_Identity=27.5, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787894, Length=266, Percent_Identity=28.1954887218045, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790551, Length=278, Percent_Identity=29.136690647482, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1786600, Length=207, Percent_Identity=28.0193236714976, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790346, Length=237, Percent_Identity=26.5822784810127, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789808, Length=280, Percent_Identity=25.3571428571429, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790861, Length=202, Percent_Identity=29.2079207920792, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786912, Length=204, Percent_Identity=28.921568627451, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789403, Length=209, Percent_Identity=27.7511961722488, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790300, Length=221, Percent_Identity=30.316742081448, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788713, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787374, Length=228, Percent_Identity=25, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=24.8945147679325, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789149, Length=215, Percent_Identity=29.3023255813954, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49821; Mature: 49821

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEYQLDLHLDAEKT
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHH
RYQQMALTLDKRSFATQVRSADPKVTLIVWRDSSDYIGSLSMIPDDMPMLPEKREFPVFT
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCEEE
GGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNAAITALMLTVVLTLALGYLFS
CCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAILRRLVQYNRLSKKIERGQYSTRLPVSWRQDEFDMLAKQFNGVLDALEKNLAAVRGVT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEESCAILTEELDHCLATFDAMLSLTRIEEGQ
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QTLELQDVSLIAISQDLFEMAEAMAEFNDQSLKLTTGEEFQVSGDKYLLFQALFNLVDNA
HHEECCCCEEEEHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCHHHHHHHHHHHHHHH
IKYSGEGAEISISQNRNVISIQDNGPGIPEGSRDKVFERLVRLDPSRHHKGTGLGLSMVK
HHCCCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
AILSRHNAKIALTDNQPGLKVIITF
HHHHCCCCEEEEECCCCCCEEEEEC
>Mature Secondary Structure
MLGIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEYQLDLHLDAEKT
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHH
RYQQMALTLDKRSFATQVRSADPKVTLIVWRDSSDYIGSLSMIPDDMPMLPEKREFPVFT
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCEEE
GGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNAAITALMLTVVLTLALGYLFS
CCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAILRRLVQYNRLSKKIERGQYSTRLPVSWRQDEFDMLAKQFNGVLDALEKNLAAVRGVT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEESCAILTEELDHCLATFDAMLSLTRIEEGQ
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QTLELQDVSLIAISQDLFEMAEAMAEFNDQSLKLTTGEEFQVSGDKYLLFQALFNLVDNA
HHEECCCCEEEEHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCHHHHHHHHHHHHHHH
IKYSGEGAEISISQNRNVISIQDNGPGIPEGSRDKVFERLVRLDPSRHHKGTGLGLSMVK
HHCCCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
AILSRHNAKIALTDNQPGLKVIITF
HHHHCCCCEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]