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The map label for this gene is menF [H]

Identifier: 157963900

GI number: 157963900

Start: 4971514

End: 4972872

Strand: Direct

Name: menF [H]

Synonym: Spea_4089

Alternate gene names: 157963900

Gene position: 4971514-4972872 (Clockwise)

Preceding gene: 157963892

Following gene: 157963901

Centisome position: 96.08

GC content: 49.37

Gene sequence:

>1359_bases
TTGACCATCCCCTTGCTGCCTCAAGCTATTAAGTCTTTAACCGACAAAATCAAATCCTTTACTCTCCAACCAAACAGCGA
CCCAATTGCACAGCTGTCTGTGGTGATTTCGCCGATCCCGGTTATTGCCTGGCTATCGGCGCAAACAACTTACCCGAGAG
TGTATTGGAAGGGACGTGATACCGAAGAAGAGGTAGCCGCCATCGGTTGCTGTAAGGACTTTTTCTTTAATGATGATGTC
GATGATATAGAGCTAGCCGCCGAGTACCAAAAACAGCGAGCACTCACCAGCAATCAGGAGATCCGCTACTATGGTGGTGT
CGCCTTCGATCGTCGCAATGAAAGCTGGCCGGAGTTTGGTAAGGCGCACTTCGTGCTGCCTCGCATCGAACTACGCCGCA
GTGGTAACGAATATCGACTCTTGGTTAATCTTAACTTTAGCGAGAATAGCTTCGCTGAAGAGATTGAACTGGCTGTGCAG
TCACTGCAGAAATTAGACTCACCTAAGCCACTCTCCCCGCCTAATAAAGTGGCACTTATGGGACGCAGTGATAGACCCGA
CAGATACCGCTGGAACGAGCTGGTTAATCGTGTCACTCATCCTAAATTTATCCAAGATACGCCTAAAGTTGTTCTGTCTC
GAGTCACCCAACTTGAGATAAATGAAGAGGTGGATCCTTGGATGCTACTCGCCTGCTGGCAGGGCCGTAATCCAAATAGC
TTTCAATTTGGCTTTCAATTTAGTCCAGAAAGCGCCTTTATCTCTTGTACACCAGAGCGCTTATATCGTCGTCGTCAACG
AGAGCTGTTTACCGAAGCCCTCGCAGGCACCACGGTTCGAGGCCTGAACCAAGAACAAGATAAAGCACTGGCACAAGCAC
TGCTAGATGACAGCAAAAACAGCCATGAGAATCAGCTGGTTCGGCAGCATATCGTCGATGTGCTCAATCCACTGAGTAAC
TACGTGGGCGCAGAAGAGACACCGACTATATTTAAGTTAAGCCATATTCAGCATCTGCATCGCTCTATTCGGGCCGAGCT
TAAAGCCGGTGTCAGTGATTTTCAGGTGATTCAGGCACTGCACCCTACTCCTGCGGTCGGTGGACTCCCGAAAGAGTCTG
CGATGAGCTTTATTCGTCAGCAAGAGGGCTACGTTAGAGGTTGGTACGCAGGTGCTTGCGGCTATTTCAACAAGTACGAA
AGCGAGTTTGCCGTCGCCATCCGCAGCGCGCTTATCGAGCCTGGCCGTATTAATCTATTTGCTGGCGCAGGTATTGTCGC
GGGTTCAGAGGCCGATAAAGAGTGGGTCGAGCTAGAGAATAAACTCACTACCATCTTGTCTATCTTGACAGAATTGTAA

Upstream 100 bases:

>100_bases
CTTTGTGGTAAAGATTCTTATTACCAATGTGCGATGGGTGTATCCCAATTAGAGGCAGCTCCTCTAAGCAAGCCCTTATG
ATATTTGTTTGGAGTACTTA

Downstream 100 bases:

>100_bases
TTCCTCAAGGTAAAATCAATTACTTAATCAAGTTCGACGGCCGATCTTTATCGGTCGTCAAGATTTCCTCTTGCTCAAAT
AAAAATCTTCTCTATAATTT

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTIPLLPQAIKSLTDKIKSFTLQPNSDPIAQLSVVISPIPVIAWLSAQTTYPRVYWKGRDTEEEVAAIGCCKDFFFNDDV
DDIELAAEYQKQRALTSNQEIRYYGGVAFDRRNESWPEFGKAHFVLPRIELRRSGNEYRLLVNLNFSENSFAEEIELAVQ
SLQKLDSPKPLSPPNKVALMGRSDRPDRYRWNELVNRVTHPKFIQDTPKVVLSRVTQLEINEEVDPWMLLACWQGRNPNS
FQFGFQFSPESAFISCTPERLYRRRQRELFTEALAGTTVRGLNQEQDKALAQALLDDSKNSHENQLVRQHIVDVLNPLSN
YVGAEETPTIFKLSHIQHLHRSIRAELKAGVSDFQVIQALHPTPAVGGLPKESAMSFIRQQEGYVRGWYAGACGYFNKYE
SEFAVAIRSALIEPGRINLFAGAGIVAGSEADKEWVELENKLTTILSILTEL

Sequences:

>Translated_452_residues
MTIPLLPQAIKSLTDKIKSFTLQPNSDPIAQLSVVISPIPVIAWLSAQTTYPRVYWKGRDTEEEVAAIGCCKDFFFNDDV
DDIELAAEYQKQRALTSNQEIRYYGGVAFDRRNESWPEFGKAHFVLPRIELRRSGNEYRLLVNLNFSENSFAEEIELAVQ
SLQKLDSPKPLSPPNKVALMGRSDRPDRYRWNELVNRVTHPKFIQDTPKVVLSRVTQLEINEEVDPWMLLACWQGRNPNS
FQFGFQFSPESAFISCTPERLYRRRQRELFTEALAGTTVRGLNQEQDKALAQALLDDSKNSHENQLVRQHIVDVLNPLSN
YVGAEETPTIFKLSHIQHLHRSIRAELKAGVSDFQVIQALHPTPAVGGLPKESAMSFIRQQEGYVRGWYAGACGYFNKYE
SEFAVAIRSALIEPGRINLFAGAGIVAGSEADKEWVELENKLTTILSILTEL
>Mature_451_residues
TIPLLPQAIKSLTDKIKSFTLQPNSDPIAQLSVVISPIPVIAWLSAQTTYPRVYWKGRDTEEEVAAIGCCKDFFFNDDVD
DIELAAEYQKQRALTSNQEIRYYGGVAFDRRNESWPEFGKAHFVLPRIELRRSGNEYRLLVNLNFSENSFAEEIELAVQS
LQKLDSPKPLSPPNKVALMGRSDRPDRYRWNELVNRVTHPKFIQDTPKVVLSRVTQLEINEEVDPWMLLACWQGRNPNSF
QFGFQFSPESAFISCTPERLYRRRQRELFTEALAGTTVRGLNQEQDKALAQALLDDSKNSHENQLVRQHIVDVLNPLSNY
VGAEETPTIFKLSHIQHLHRSIRAELKAGVSDFQVIQALHPTPAVGGLPKESAMSFIRQQEGYVRGWYAGACGYFNKYES
EFAVAIRSALIEPGRINLFAGAGIVAGSEADKEWVELENKLTTILSILTEL

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=427, Percent_Identity=34.6604215456674, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786809, Length=265, Percent_Identity=27.9245283018868, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787518, Length=223, Percent_Identity=29.1479820627803, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788114, Length=368, Percent_Identity=24.4565217391304, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320935, Length=324, Percent_Identity=28.7037037037037, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324361, Length=292, Percent_Identity=22.9452054794521, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51274; Mature: 51142

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPLLPQAIKSLTDKIKSFTLQPNSDPIAQLSVVISPIPVIAWLSAQTTYPRVYWKGRD
CCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCEEEECCCC
TEEEVAAIGCCKDFFFNDDVDDIELAAEYQKQRALTSNQEIRYYGGVAFDRRNESWPEFG
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCCCCCCCC
KAHFVLPRIELRRSGNEYRLLVNLNFSENSFAEEIELAVQSLQKLDSPKPLSPPNKVALM
CCEEEECHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
GRSDRPDRYRWNELVNRVTHPKFIQDTPKVVLSRVTQLEINEEVDPWMLLACWQGRNPNS
CCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
FQFGFQFSPESAFISCTPERLYRRRQRELFTEALAGTTVRGLNQEQDKALAQALLDDSKN
EEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCC
SHENQLVRQHIVDVLNPLSNYVGAEETPTIFKLSHIQHLHRSIRAELKAGVSDFQVIQAL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
HPTPAVGGLPKESAMSFIRQQEGYVRGWYAGACGYFNKYESEFAVAIRSALIEPGRINLF
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGAGIVAGSEADKEWVELENKLTTILSILTEL
ECCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIPLLPQAIKSLTDKIKSFTLQPNSDPIAQLSVVISPIPVIAWLSAQTTYPRVYWKGRD
CCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCEEEECCCC
TEEEVAAIGCCKDFFFNDDVDDIELAAEYQKQRALTSNQEIRYYGGVAFDRRNESWPEFG
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCCCCCCCC
KAHFVLPRIELRRSGNEYRLLVNLNFSENSFAEEIELAVQSLQKLDSPKPLSPPNKVALM
CCEEEECHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
GRSDRPDRYRWNELVNRVTHPKFIQDTPKVVLSRVTQLEINEEVDPWMLLACWQGRNPNS
CCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
FQFGFQFSPESAFISCTPERLYRRRQRELFTEALAGTTVRGLNQEQDKALAQALLDDSKN
EEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCC
SHENQLVRQHIVDVLNPLSNYVGAEETPTIFKLSHIQHLHRSIRAELKAGVSDFQVIQAL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
HPTPAVGGLPKESAMSFIRQQEGYVRGWYAGACGYFNKYESEFAVAIRSALIEPGRINLF
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGAGIVAGSEADKEWVELENKLTTILSILTEL
ECCCEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]