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The map label for this gene is pgaB [H]

Identifier: 157963819

GI number: 157963819

Start: 4868294

End: 4869340

Strand: Reverse

Name: pgaB [H]

Synonym: Spea_4008

Alternate gene names: 157963819

Gene position: 4869340-4868294 (Counterclockwise)

Preceding gene: 157963821

Following gene: 157963807

Centisome position: 94.1

GC content: 47.85

Gene sequence:

>1047_bases
ATGGTTAAAAACGTGTTGCTAGCGCTACTTGCGCTGACAGGTTTTTCGGCTCACGCGACGGTGATCCTACAGTATCATCA
TGTTTCGGATAGTACGCCAGCGATCACCAGTGTTACTCCTGCGCAATTTAAAGAGCAGATGCAGTATCTGGCGGAAAACA
ACTTCAACGTCGTGCCACTTTCAACTGTGGTGGAGTCAATTAAGGCTAAGAAAGCACTGCCAGCAAAAACGATTGCGATC
ACCTTCGATGATGGTTACCGCAGCATTGCTAATACAGCGCACCCCATTTTAAAGCAATATAAATTCCCCTACACCCTTTT
TGTCTCGGTTGAACCGATCCTAAAAGAGTATGGCGAAATGATGAGCTGGCAGCAGCTGATCACGCTATCTAACGAGGGAG
CGGAAATTGCCAACCATAGTTGGGGGCATGAACACCTTATTCGTAAAGAGGCGGGCGAAACGGATAAGCAATGGCTAGCA
CGTATCGAGAGCAATATTTTGCGCACTGAAGCCGAGATTGCTAAGGCGACAGGGCAAAACTTGAAAATGCTGGCTTACCC
ATACGGTGAATACAATCGGCAGATTGAAGATATGCTCGACAAGCACGGCTTTGTGGCGTTTGGTCAGCAATCTGGAGCGG
CAGGTCCATACTCGCCATTAACCGCGTTACCTCGCTTTCCTGTAGCAGGTGTTTATGCCGACCTCAACAGCCTAAAGGTA
AAGCTGCATAGTTTGAATATGCCTGTGCTGGCTCAAACGAATAGCAACCCTGAGTTGGCTCAAGGCCAATGGCGACCAGA
GATTAAGGTGACCCTAGATATGAGTGATATCAATGCGTCACAGCTGATGTGCTTTATTCAGGGACAAGGTGCTAAGAAGC
CAAGTTGGATCTCTGATAATGAGTTTAGTATTCAGGCCGAGCTTGATTTGCCTGCGGGGCGCTCACGTTATAACTGTACC
GCGCCAAGTAAGAGTAAAGGTAGTTACTACTGGTTCTCACAGCCTTGGATTAGGCCTAAGTCAAACGGTCAGTGGATTGC
TGAATAG

Upstream 100 bases:

>100_bases
CCGTGATATTTGATAAAATCATCGGTAGTGCGAGGGCAAACAGTTGTCGGTTTTTTTGTGGGTTTAACAGTAAGGCAATC
ATTTTAGAGGTCTAATATTT

Downstream 100 bases:

>100_bases
CTTTTAAAGCAAAATAAAACGTCATCATTGGCGTTTTTTTTTGCTCGAATTTTACGATGTGTATTAACCGGAATACTTAT
CAATAATTGCATTGACGTCA

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MVKNVLLALLALTGFSAHATVILQYHHVSDSTPAITSVTPAQFKEQMQYLAENNFNVVPLSTVVESIKAKKALPAKTIAI
TFDDGYRSIANTAHPILKQYKFPYTLFVSVEPILKEYGEMMSWQQLITLSNEGAEIANHSWGHEHLIRKEAGETDKQWLA
RIESNILRTEAEIAKATGQNLKMLAYPYGEYNRQIEDMLDKHGFVAFGQQSGAAGPYSPLTALPRFPVAGVYADLNSLKV
KLHSLNMPVLAQTNSNPELAQGQWRPEIKVTLDMSDINASQLMCFIQGQGAKKPSWISDNEFSIQAELDLPAGRSRYNCT
APSKSKGSYYWFSQPWIRPKSNGQWIAE

Sequences:

>Translated_348_residues
MVKNVLLALLALTGFSAHATVILQYHHVSDSTPAITSVTPAQFKEQMQYLAENNFNVVPLSTVVESIKAKKALPAKTIAI
TFDDGYRSIANTAHPILKQYKFPYTLFVSVEPILKEYGEMMSWQQLITLSNEGAEIANHSWGHEHLIRKEAGETDKQWLA
RIESNILRTEAEIAKATGQNLKMLAYPYGEYNRQIEDMLDKHGFVAFGQQSGAAGPYSPLTALPRFPVAGVYADLNSLKV
KLHSLNMPVLAQTNSNPELAQGQWRPEIKVTLDMSDINASQLMCFIQGQGAKKPSWISDNEFSIQAELDLPAGRSRYNCT
APSKSKGSYYWFSQPWIRPKSNGQWIAE
>Mature_348_residues
MVKNVLLALLALTGFSAHATVILQYHHVSDSTPAITSVTPAQFKEQMQYLAENNFNVVPLSTVVESIKAKKALPAKTIAI
TFDDGYRSIANTAHPILKQYKFPYTLFVSVEPILKEYGEMMSWQQLITLSNEGAEIANHSWGHEHLIRKEAGETDKQWLA
RIESNILRTEAEIAKATGQNLKMLAYPYGEYNRQIEDMLDKHGFVAFGQQSGAAGPYSPLTALPRFPVAGVYADLNSLKV
KLHSLNMPVLAQTNSNPELAQGQWRPEIKVTLDMSDINASQLMCFIQGQGAKKPSWISDNEFSIQAELDLPAGRSRYNCT
APSKSKGSYYWFSQPWIRPKSNGQWIAE

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=260, Percent_Identity=23.8461538461538, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786322, Length=211, Percent_Identity=28.436018957346, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 38852; Mature: 38852

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKNVLLALLALTGFSAHATVILQYHHVSDSTPAITSVTPAQFKEQMQYLAENNFNVVPL
CHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEH
STVVESIKAKKALPAKTIAITFDDGYRSIANTAHPILKQYKFPYTLFVSVEPILKEYGEM
HHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHH
MSWQQLITLSNEGAEIANHSWGHEHLIRKEAGETDKQWLARIESNILRTEAEIAKATGQN
HHHHHHHEECCCCCHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
LKMLAYPYGEYNRQIEDMLDKHGFVAFGQQSGAAGPYSPLTALPRFPVAGVYADLNSLKV
CEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHCHHHEEE
KLHSLNMPVLAQTNSNPELAQGQWRPEIKVTLDMSDINASQLMCFIQGQGAKKPSWISDN
EEEECCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHEEEEEECCCCCCCCCCCCC
EFSIQAELDLPAGRSRYNCTAPSKSKGSYYWFSQPWIRPKSNGQWIAE
CEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECC
>Mature Secondary Structure
MVKNVLLALLALTGFSAHATVILQYHHVSDSTPAITSVTPAQFKEQMQYLAENNFNVVPL
CHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEH
STVVESIKAKKALPAKTIAITFDDGYRSIANTAHPILKQYKFPYTLFVSVEPILKEYGEM
HHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHH
MSWQQLITLSNEGAEIANHSWGHEHLIRKEAGETDKQWLARIESNILRTEAEIAKATGQN
HHHHHHHEECCCCCHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
LKMLAYPYGEYNRQIEDMLDKHGFVAFGQQSGAAGPYSPLTALPRFPVAGVYADLNSLKV
CEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHCHHHEEE
KLHSLNMPVLAQTNSNPELAQGQWRPEIKVTLDMSDINASQLMCFIQGQGAKKPSWISDN
EEEECCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHEEEEEECCCCCCCCCCCCC
EFSIQAELDLPAGRSRYNCTAPSKSKGSYYWFSQPWIRPKSNGQWIAE
CEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]