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The map label for this gene is plsB [H]

Identifier: 157963807

GI number: 157963807

Start: 4854636

End: 4857059

Strand: Reverse

Name: plsB [H]

Synonym: Spea_3996

Alternate gene names: 157963807

Gene position: 4857059-4854636 (Counterclockwise)

Preceding gene: 157963819

Following gene: 157963806

Centisome position: 93.86

GC content: 46.78

Gene sequence:

>2424_bases
ATGTCCAAACAAGACTCTCTTTGGTTCAAATCTTTACGCTGGTTACAGCGTCAATTAGTTCACACTGTCGTTGTGCCTCA
CGACCCATTCGACGATCTCAACTTAGATCCGAGTAAACCACTCGCTTATGTCATGAAGACTGAATCTGTCAGTGACATCG
CTGCGCTTAGCGAAATGACGGCGAAACTTGGTTTACCAAGCCCTTACGAGGAGCTGGAGGTCAATGGGGTTAAGGCACCA
CGAGTGGTCTGCCTAGAAGGGAGTAAGCCTCTCTTCGGCCAGCGAGAAGGTGGTGAGCAGTACATAGATTGCTTCAAGAG
ACTCCTTAGCGTACATAAACAAAATCGCGAATTAGACATTCAATTGGTGCCGGTGAGCCTTTATTGGGGTCGTACCCCTG
GTAAAGAAGACGACACTATGCGCGCAGCGGTACTCGAGCGCCAAAACCCAACTTGGTTACGCAAGTGCCTCATGATCTTG
TTCTTGGGTCGACATAATTTCGTGCAGTTTTCTAATGCCGTTTCACTGCGTTATATGGCTGATGAGCACGGCACTGATAA
GCGTATTGCACAGAAGCTTGCTCGTGTCGCAAGAGTGCACTTTCAGCGCCAACGTAAAGTGATGACTGGGCCTCAGCTGC
CTAAGCGTCAGGCACTGTTCCACGCTTTACTTAAATCAGATTCGATTACCAAGGCGATTAAAGAGGAAGCGGCAAGCAAG
AAGATCACCGAGGCCGAAGCTCGCGCTAAAGCGATGGAGTACCTTGATGAAGTGGCTGCAGATTACTCCGATAGCCTAGT
GCGTATCGCAGAGCGTTTTCTAACGTGGTTGTGGAACAAGCTTTATAAAGGCATCAACATTAAAGGTGCCGAGCAGGTAA
GACAGCTGCACCACGACGGACATGAGATTGTCTATGTGCCGTGTCATCGTAGTCATATGGACTACCTGCTACTGTCTTAT
ATTCTTTATTATCAAGGTATGGTACCGCCGCATATCGCAGCGGGTATTAACCTTAATTTCTGGCCTGCGGGTCCTGCGTT
CCGCCGTGGTGGTGCTTTCTTTATTCGTCGTAGTTTTGGTGGCAACAAGCTTTACACTGCCGTGTTCCGTGAATATTTAG
ATCAGCTATTTACCAAAGGCTATTCTGTAGAGTACTTCACAGAAGGTGGCCGTTCGCGTACAGGTCGTCTGCTGGCGCCG
AAAACGGGAATGCTCGCTATGACCCTAAACAGTGTGCTGCGTGGTGTTGAACGCCCAGTGACGCTAGTGCCAGTGTATTT
AGGCTATGACCATGTAATGGAAGTGGCGACTTACCATAAAGAGTTGAGCGGAAAGAAGAAAAAGAAAGAGTCAGTTTGGC
AGGTATTTGGCGCCATTCGTAAGCTAGGCAACTTTGGCCAAGGTTACGTTAACTTCGGTGAGCCGATCACCTTGCATAAC
TTCTTGAACGAGCAGGCTCCTAGCTGGCGTGAAGATATAGCTAAAGACCCAGACCAAAAGCCTAGCTGGTTAACGCCTGT
GGTGAACACATTGGCTAATCGTGTGATGACTAACATCAACGATGCGGCGGCGGTGAGCTCTGTGACCTTAACTAGTATGG
TGTTACTCGCTTCTGAGCAAAATGCGCTTGAGCGTTCTCAACTAGAGAAGCAGTTAGACTTGTATCTAACCATATTAAAA
GAGCAGCCATATACCGAGTATACGTCTGTGCCAGAAGGTACGGGGCATGACTTGGTTTCTCAAGGTTTAGAGCTTAAAAA
GTTGCAGATTGAAAGCGATCCCCTAGGCGATATCATTTCGATTGATCAAAGCATCGCGATCACCATGACTTACTATCGTA
ATAACATCATCCACCTTATGGTGTTGCCATCTTTGATTGCGGCATGTTTGCTGCGTAAAGAAAATTGCAGCCGTGAAGAT
GTTATCTGTGTTGTGAATGACTTCTATCCATTGCTTGAAGCCGAATTGTTTATGGGTATTGATGACCCTGCTCAATACGC
AAGCCAGATCCTCGACATCTTTGTTGCTCAAGGTCTTGTGGTTGAAGCAGAGCACTTCGAAGTTGTGGAGAGCAAGATTA
ATCAATTGCTACTGCTTTCTGGCACTATCAGTGAAACCATGCAGCGTTACGCTATCTTGTTTAACTTGCTTGAAGTTAAG
CCAAATATGGAGCGTTCAGAGCTTGAAAAAGACAGTCATCGCTTGGCACAAAGATTAGGCGCACTTCACGGTATTACTGC
ACCAGAGTTTTATGACAAGAAGCTATACGCTACGCTGAGTGTCAAATTAAAAGAGCTAGGTTACCTAGCTGATAATCAAG
GGTGCAGCGATATCAAACGTATTAAAGAGAGAGCGAATCTACTGTTACGCTCGTCCGTAAGGCAAACGATTGTAGATAGT
GTGCATGCGGAGCAGAATACTTAA

Upstream 100 bases:

>100_bases
ACAAACCGGATTCGGTACGGTAAGAAGAGAATTGCGCAAACTATTTCAATATCTGCTATTCTAGCGCCTTATTAGAAGCG
ACTAACATTGCAAATAAATC

Downstream 100 bases:

>100_bases
TGGCAAATCATATGAATGCAGCCAAGAACAAGGTTGCAGGTAAGTCGCTGTTAGGTGGGGCCATGATTATTGCGGGCACA
GCTGTCGGCGCAGGCATGTT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSKQDSLWFKSLRWLQRQLVHTVVVPHDPFDDLNLDPSKPLAYVMKTESVSDIAALSEMTAKLGLPSPYEELEVNGVKAP
RVVCLEGSKPLFGQREGGEQYIDCFKRLLSVHKQNRELDIQLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMIL
FLGRHNFVQFSNAVSLRYMADEHGTDKRIAQKLARVARVHFQRQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASK
KITEAEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDGHEIVYVPCHRSHMDYLLLSY
ILYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFGGNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAP
KTGMLAMTLNSVLRGVERPVTLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQVFGAIRKLGNFGQGYVNFGEPITLHN
FLNEQAPSWREDIAKDPDQKPSWLTPVVNTLANRVMTNINDAAAVSSVTLTSMVLLASEQNALERSQLEKQLDLYLTILK
EQPYTEYTSVPEGTGHDLVSQGLELKKLQIESDPLGDIISIDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCSRED
VICVVNDFYPLLEAELFMGIDDPAQYASQILDIFVAQGLVVEAEHFEVVESKINQLLLLSGTISETMQRYAILFNLLEVK
PNMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLADNQGCSDIKRIKERANLLLRSSVRQTIVDS
VHAEQNT

Sequences:

>Translated_807_residues
MSKQDSLWFKSLRWLQRQLVHTVVVPHDPFDDLNLDPSKPLAYVMKTESVSDIAALSEMTAKLGLPSPYEELEVNGVKAP
RVVCLEGSKPLFGQREGGEQYIDCFKRLLSVHKQNRELDIQLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMIL
FLGRHNFVQFSNAVSLRYMADEHGTDKRIAQKLARVARVHFQRQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASK
KITEAEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDGHEIVYVPCHRSHMDYLLLSY
ILYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFGGNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAP
KTGMLAMTLNSVLRGVERPVTLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQVFGAIRKLGNFGQGYVNFGEPITLHN
FLNEQAPSWREDIAKDPDQKPSWLTPVVNTLANRVMTNINDAAAVSSVTLTSMVLLASEQNALERSQLEKQLDLYLTILK
EQPYTEYTSVPEGTGHDLVSQGLELKKLQIESDPLGDIISIDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCSRED
VICVVNDFYPLLEAELFMGIDDPAQYASQILDIFVAQGLVVEAEHFEVVESKINQLLLLSGTISETMQRYAILFNLLEVK
PNMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLADNQGCSDIKRIKERANLLLRSSVRQTIVDS
VHAEQNT
>Mature_806_residues
SKQDSLWFKSLRWLQRQLVHTVVVPHDPFDDLNLDPSKPLAYVMKTESVSDIAALSEMTAKLGLPSPYEELEVNGVKAPR
VVCLEGSKPLFGQREGGEQYIDCFKRLLSVHKQNRELDIQLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMILF
LGRHNFVQFSNAVSLRYMADEHGTDKRIAQKLARVARVHFQRQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASKK
ITEAEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDGHEIVYVPCHRSHMDYLLLSYI
LYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFGGNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAPK
TGMLAMTLNSVLRGVERPVTLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQVFGAIRKLGNFGQGYVNFGEPITLHNF
LNEQAPSWREDIAKDPDQKPSWLTPVVNTLANRVMTNINDAAAVSSVTLTSMVLLASEQNALERSQLEKQLDLYLTILKE
QPYTEYTSVPEGTGHDLVSQGLELKKLQIESDPLGDIISIDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCSREDV
ICVVNDFYPLLEAELFMGIDDPAQYASQILDIFVAQGLVVEAEHFEVVESKINQLLLLSGTISETMQRYAILFNLLEVKP
NMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLADNQGCSDIKRIKERANLLLRSSVRQTIVDSV
HAEQNT

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=445, Percent_Identity=30.1123595505618, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI190358539, Length=421, Percent_Identity=26.3657957244656, Blast_Score=141, Evalue=3e-33,
Organism=Escherichia coli, GI87082362, Length=814, Percent_Identity=47.9115479115479, Blast_Score=771, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=352, Percent_Identity=32.1022727272727, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71988723, Length=448, Percent_Identity=27.0089285714286, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71988728, Length=389, Percent_Identity=28.7917737789203, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17864692, Length=375, Percent_Identity=29.3333333333333, Blast_Score=155, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24650754, Length=419, Percent_Identity=28.1622911694511, Blast_Score=130, Evalue=5e-30,
Organism=Drosophila melanogaster, GI21357731, Length=341, Percent_Identity=29.6187683284457, Blast_Score=130, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24650752, Length=419, Percent_Identity=28.1622911694511, Blast_Score=130, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 91657; Mature: 91526

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQDSLWFKSLRWLQRQLVHTVVVPHDPFDDLNLDPSKPLAYVMKTESVSDIAALSEMT
CCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
AKLGLPSPYEELEVNGVKAPRVVCLEGSKPLFGQREGGEQYIDCFKRLLSVHKQNRELDI
HHCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
QLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMILFLGRHNFVQFSNAVSLRYMA
EEEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEC
DEHGTDKRIAQKLARVARVHFQRQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
KITEAEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDG
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
HEIVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFG
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCHHHHCCCEEEEEECCC
GNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAPKTGMLAMTLNSVLRGVERPV
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCE
TLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQVFGAIRKLGNFGQGYVNFGEPITLHN
EEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
FLNEQAPSWREDIAKDPDQKPSWLTPVVNTLANRVMTNINDAAAVSSVTLTSMVLLASEQ
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCH
NALERSQLEKQLDLYLTILKEQPYTEYTSVPEGTGHDLVSQGLELKKLQIESDPLGDIIS
HHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCHHC
IDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCSREDVICVVNDFYPLLEAELFMGI
CCCCEEEEEEEHHCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCC
DDPAQYASQILDIFVAQGLVVEAEHFEVVESKINQLLLLSGTISETMQRYAILFNLLEVK
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCC
PNMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLADNQGCSDIKR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
IKERANLLLRSSVRQTIVDSVHAEQNT
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKQDSLWFKSLRWLQRQLVHTVVVPHDPFDDLNLDPSKPLAYVMKTESVSDIAALSEMT
CCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
AKLGLPSPYEELEVNGVKAPRVVCLEGSKPLFGQREGGEQYIDCFKRLLSVHKQNRELDI
HHCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
QLVPVSLYWGRTPGKEDDTMRAAVLERQNPTWLRKCLMILFLGRHNFVQFSNAVSLRYMA
EEEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEEEC
DEHGTDKRIAQKLARVARVHFQRQRKVMTGPQLPKRQALFHALLKSDSITKAIKEEAASK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
KITEAEARAKAMEYLDEVAADYSDSLVRIAERFLTWLWNKLYKGINIKGAEQVRQLHHDG
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
HEIVYVPCHRSHMDYLLLSYILYYQGMVPPHIAAGINLNFWPAGPAFRRGGAFFIRRSFG
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCCHHHHCCCEEEEEECCC
GNKLYTAVFREYLDQLFTKGYSVEYFTEGGRSRTGRLLAPKTGMLAMTLNSVLRGVERPV
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCE
TLVPVYLGYDHVMEVATYHKELSGKKKKKESVWQVFGAIRKLGNFGQGYVNFGEPITLHN
EEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
FLNEQAPSWREDIAKDPDQKPSWLTPVVNTLANRVMTNINDAAAVSSVTLTSMVLLASEQ
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCH
NALERSQLEKQLDLYLTILKEQPYTEYTSVPEGTGHDLVSQGLELKKLQIESDPLGDIIS
HHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCHHC
IDQSIAITMTYYRNNIIHLMVLPSLIAACLLRKENCSREDVICVVNDFYPLLEAELFMGI
CCCCEEEEEEEHHCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCC
DDPAQYASQILDIFVAQGLVVEAEHFEVVESKINQLLLLSGTISETMQRYAILFNLLEVK
CCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHCC
PNMERSELEKDSHRLAQRLGALHGITAPEFYDKKLYATLSVKLKELGYLADNQGCSDIKR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
IKERANLLLRSSVRQTIVDSVHAEQNT
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA