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The map label for this gene is menD [H]

Identifier: 157963782

GI number: 157963782

Start: 4824773

End: 4826500

Strand: Direct

Name: menD [H]

Synonym: Spea_3971

Alternate gene names: 157963782

Gene position: 4824773-4826500 (Clockwise)

Preceding gene: 157963780

Following gene: 157963783

Centisome position: 93.24

GC content: 49.25

Gene sequence:

>1728_bases
ATGATACGGTACTCATCTATGCAAACTGAACACACCGCTGAACTTAATCTGCTTTGGGGAACTTTAATTTTAGAGGAGTT
GGCTCGATTAGGCGTTAAACATGTCTGTATGGCGCCAGGTTCTCGCTCCACCCCACTTACCCTTGCTGCGGCGAAGCAAA
CTAAGCTTAGCCAACACATTCATTTTGATGAGCGAGGACTCGGCTTTATGGCGCTTGGTTTGGCTAAGGCCAGTCAAGCA
CCTGTTGCGATAATTACCACTTCTGGTACCGCGGTTGCCAATCTTTACCCTGCAGTGATAGAAGCTTGGCTTACTCACGT
ACCGTTAATTGTATTATCTGGCGATAGACCTCCAGAGCTTATTGACTGCGGCGCCAATCAGGCGATCATTCAGCCTGCCA
TTTTTGCTCAGTATGCCAAACAGATAAATCTACCGACCCCAGATCTCAATATTGCTCCTCAGGCACTGCTAAGCCTGCTA
GATGAAGCGATTTGCAACCAATCACAGCCGGTTCATATCAACTGTATGTACCGTGAGCCTTTGTACCCTAATGAACCTAA
GGTAGATTTTGAGCATTACTTAGCGCCAGTTAAACAGTGGCAACAACACACAGCGCCCTATAGCCAATTTGCCAATCTAA
CTAGCGGCCTGATGCCAACAGCCGATGCACTCGCGCGCTTTGTACATGGTAAAGGTGTGATTGTAGCGGCAACCTTAGCC
CCTGAGCAGAAGCCAGAGCAGCTGATTGAACTCGCGCAAAAGCTAGGTTGGCCAATCGTCACCGATGCCCAGTCGCAGCT
CAGGCAGCATCCTGCAGCCATAGGTAATATCGATCAGCTACTGCATCAACCAAAATCTAAGGCTCTGCTCGCTCAGGCAG
AAAGAGTCATCGTCTTCGGTGGTCGTATTCTTTCTAAACGGCTTATCGGCTATCTAGCTGAGCAGAATTGGAAAGATTAT
TGGCAGGTGCTACCACAGCAACAGAGGCTAGACCCGAGCCACAAACCTAAAAAGGTTTGGATTGCAGCTGCGCACCAATT
TGCCAAGCTCGCTTGGCCAAGATCCTCACAGGCCAATTGGGCACTCAAGCTTGTACAGCACAACGATGATCTAGAAACGC
TGTATCAACAGCAAATTGATAACGCCGAATTTGGTGAGGCGCAGACGATTCGTGCTATTGCTAAGCGACAAACCGCTGAG
CAGCACCTGTTTATCGGCAATAGTCTACCAGTGCGCCTATATGACATGTTTGCGCCTATTACCACTTCAAGTGCCAATGT
ATTTACCAATCGCGGCGCATCGGGTATCGATGGCTTATTAGCAACGGCCTGCGGAGTCGCGGCAGCAGAGGGCAAGCCGA
CCAGCCTGATTATTGGTGATATATCGGCGCTGCACGATCTCAACTCATTAGCCATTGCACGCTCGGTCACCAGCCCGCTA
GTAATCGTCATACTCAATAATGACGGCGGTAATATCTTTAACCTGCTTCCGGTGCCTAACGAGCAGCTCAGAACCGACTA
CTATCGCCTGTCTCACGGGTTGGAGTTTGGTTTCGGTGCCGCCATGTTCGGCCTTGCTTATAATCAAGTCGATTCAATTA
GCGACTTTATTGAGTCTTACGAATACGCCATGACCTATTCAGGCCCCTCGGTTATTGAGGTTACTGTGGCACAAGACCAA
GCCAGTGAGCAGATAGCTCAAATAGCTAGCTGGGTAAAACAGAGCTAA

Upstream 100 bases:

>100_bases
AAAGATAAGGCACCTCAGTTTCAACAACTGCCCAGTGTTAAAGAAAACCTAAGTGGGATCATACTCCTTTACCACAAAGC
GAGTTAAACTATGGCTATCA

Downstream 100 bases:

>100_bases
TGCTAAGCTATCGCACCGCAGGGAAAGCTAGCTCTCCAGCCGTGGTATTTCTCCACGGCTTCTTAGGCAGTAAAGAAGAT
TGGCATGAGATCATCGAGCA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MIRYSSMQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQTKLSQHIHFDERGLGFMALGLAKASQA
PVAIITTSGTAVANLYPAVIEAWLTHVPLIVLSGDRPPELIDCGANQAIIQPAIFAQYAKQINLPTPDLNIAPQALLSLL
DEAICNQSQPVHINCMYREPLYPNEPKVDFEHYLAPVKQWQQHTAPYSQFANLTSGLMPTADALARFVHGKGVIVAATLA
PEQKPEQLIELAQKLGWPIVTDAQSQLRQHPAAIGNIDQLLHQPKSKALLAQAERVIVFGGRILSKRLIGYLAEQNWKDY
WQVLPQQQRLDPSHKPKKVWIAAAHQFAKLAWPRSSQANWALKLVQHNDDLETLYQQQIDNAEFGEAQTIRAIAKRQTAE
QHLFIGNSLPVRLYDMFAPITTSSANVFTNRGASGIDGLLATACGVAAAEGKPTSLIIGDISALHDLNSLAIARSVTSPL
VIVILNNDGGNIFNLLPVPNEQLRTDYYRLSHGLEFGFGAAMFGLAYNQVDSISDFIESYEYAMTYSGPSVIEVTVAQDQ
ASEQIAQIASWVKQS

Sequences:

>Translated_575_residues
MIRYSSMQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQTKLSQHIHFDERGLGFMALGLAKASQA
PVAIITTSGTAVANLYPAVIEAWLTHVPLIVLSGDRPPELIDCGANQAIIQPAIFAQYAKQINLPTPDLNIAPQALLSLL
DEAICNQSQPVHINCMYREPLYPNEPKVDFEHYLAPVKQWQQHTAPYSQFANLTSGLMPTADALARFVHGKGVIVAATLA
PEQKPEQLIELAQKLGWPIVTDAQSQLRQHPAAIGNIDQLLHQPKSKALLAQAERVIVFGGRILSKRLIGYLAEQNWKDY
WQVLPQQQRLDPSHKPKKVWIAAAHQFAKLAWPRSSQANWALKLVQHNDDLETLYQQQIDNAEFGEAQTIRAIAKRQTAE
QHLFIGNSLPVRLYDMFAPITTSSANVFTNRGASGIDGLLATACGVAAAEGKPTSLIIGDISALHDLNSLAIARSVTSPL
VIVILNNDGGNIFNLLPVPNEQLRTDYYRLSHGLEFGFGAAMFGLAYNQVDSISDFIESYEYAMTYSGPSVIEVTVAQDQ
ASEQIAQIASWVKQS
>Mature_575_residues
MIRYSSMQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQTKLSQHIHFDERGLGFMALGLAKASQA
PVAIITTSGTAVANLYPAVIEAWLTHVPLIVLSGDRPPELIDCGANQAIIQPAIFAQYAKQINLPTPDLNIAPQALLSLL
DEAICNQSQPVHINCMYREPLYPNEPKVDFEHYLAPVKQWQQHTAPYSQFANLTSGLMPTADALARFVHGKGVIVAATLA
PEQKPEQLIELAQKLGWPIVTDAQSQLRQHPAAIGNIDQLLHQPKSKALLAQAERVIVFGGRILSKRLIGYLAEQNWKDY
WQVLPQQQRLDPSHKPKKVWIAAAHQFAKLAWPRSSQANWALKLVQHNDDLETLYQQQIDNAEFGEAQTIRAIAKRQTAE
QHLFIGNSLPVRLYDMFAPITTSSANVFTNRGASGIDGLLATACGVAAAEGKPTSLIIGDISALHDLNSLAIARSVTSPL
VIVILNNDGGNIFNLLPVPNEQLRTDYYRLSHGLEFGFGAAMFGLAYNQVDSISDFIESYEYAMTYSGPSVIEVTVAQDQ
ASEQIAQIASWVKQS

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=560, Percent_Identity=41.25, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1790104, Length=294, Percent_Identity=25.8503401360544, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 63087; Mature: 63087

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRYSSMQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQTKLSQHI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEHHHHHHHHHC
HFDERGLGFMALGLAKASQAPVAIITTSGTAVANLYPAVIEAWLTHVPLIVLSGDRPPEL
CCCCCCCCEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHH
IDCGANQAIIQPAIFAQYAKQINLPTPDLNIAPQALLSLLDEAICNQSQPVHINCMYREP
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECC
LYPNEPKVDFEHYLAPVKQWQQHTAPYSQFANLTSGLMPTADALARFVHGKGVIVAATLA
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECC
PEQKPEQLIELAQKLGWPIVTDAQSQLRQHPAAIGNIDQLLHQPKSKALLAQAERVIVFG
CCCCHHHHHHHHHHCCCCEEECHHHHHHHCCHHCCCHHHHHHCCHHHHHHHHCCEEEEEC
GRILSKRLIGYLAEQNWKDYWQVLPQQQRLDPSHKPKKVWIAAAHQFAKLAWPRSSQANW
HHHHHHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCE
ALKLVQHNDDLETLYQQQIDNAEFGEAQTIRAIAKRQTAEQHLFIGNSLPVRLYDMFAPI
EEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCEEHHHHHCCC
TTSSANVFTNRGASGIDGLLATACGVAAAEGKPTSLIIGDISALHDLNSLAIARSVTSPL
CCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCE
VIVILNNDGGNIFNLLPVPNEQLRTDYYRLSHGLEFGFGAAMFGLAYNQVDSISDFIESY
EEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EYAMTYSGPSVIEVTVAQDQASEQIAQIASWVKQS
HHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRYSSMQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQTKLSQHI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEHHHHHHHHHC
HFDERGLGFMALGLAKASQAPVAIITTSGTAVANLYPAVIEAWLTHVPLIVLSGDRPPEL
CCCCCCCCEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHH
IDCGANQAIIQPAIFAQYAKQINLPTPDLNIAPQALLSLLDEAICNQSQPVHINCMYREP
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECC
LYPNEPKVDFEHYLAPVKQWQQHTAPYSQFANLTSGLMPTADALARFVHGKGVIVAATLA
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECC
PEQKPEQLIELAQKLGWPIVTDAQSQLRQHPAAIGNIDQLLHQPKSKALLAQAERVIVFG
CCCCHHHHHHHHHHCCCCEEECHHHHHHHCCHHCCCHHHHHHCCHHHHHHHHCCEEEEEC
GRILSKRLIGYLAEQNWKDYWQVLPQQQRLDPSHKPKKVWIAAAHQFAKLAWPRSSQANW
HHHHHHHHHHHHHHCCHHHHHHHCCHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCE
ALKLVQHNDDLETLYQQQIDNAEFGEAQTIRAIAKRQTAEQHLFIGNSLPVRLYDMFAPI
EEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCEEHHHHHCCC
TTSSANVFTNRGASGIDGLLATACGVAAAEGKPTSLIIGDISALHDLNSLAIARSVTSPL
CCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCE
VIVILNNDGGNIFNLLPVPNEQLRTDYYRLSHGLEFGFGAAMFGLAYNQVDSISDFIESY
EEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EYAMTYSGPSVIEVTVAQDQASEQIAQIASWVKQS
HHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA